Restriction map for
pF1KB7246
(Circular) (Six-base) MAPSORT of: pF1KB7246.seq Check: 4675 from: 1 to: 4673
pF1KB7246 4673 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1711 1711
Size: 4673
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 180 198 415 924 1024 1645 1669 2498
Size: 54 18 217 509 100 621 24 829
Cuts at: 2498 2930 3677 3945 126
Size: 432 747 268 854
Fragments arranged by size:
854 829 747 621 509 432 268 217
100 54 24 18
AfeI AGC'GCT
Cuts at: 147 1428 1599 147
Size: 1281 171 3221
Fragments arranged by size:
3221 1281 171
AflIII A'CryG_T
Cuts at: 896 2025 3129 4106 896
Size: 1129 1104 977 1463
Fragments arranged by size:
1463 1129 1104 977
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AleI CACnn'nnGTG
Cuts at: 728 901 728
Size: 173 4500
AlwNI CAG_nnn'CTG
Cuts at: 381 579 1122 1272 1302 1978 3545 381
Size: 198 543 150 30 676 1567 1509
Fragments arranged by size:
1567 1509 676 543 198 150 30
ApaI G_GGCC'C
Cuts at: 132 132
Size: 4673
ApaLI G'TGCA_C
Cuts at: 1389 3443 1389
Size: 2054 2619
ApoI r'AATT_y
Cuts at: 1677 4026 4282 1677
Size: 2349 256 2068
Fragments arranged by size:
2349 2068 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 1693 1693
Size: 4673
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4673
BanI G'GyrC_C
Cuts at: 245 1689 2103 2351 2386 245
Size: 1444 414 248 35 2532
Fragments arranged by size:
2532 1444 414 248 35
BanII G_rGCy'C
Cuts at: 132 332 654 1552 1687 2717 132
Size: 200 322 898 135 1030 2088
Fragments arranged by size:
2088 1030 898 322 200 135
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BbsI GAAGACnn'nnnn_
Cuts at: 1636 1657 1636
Size: 21 4652
BbvCI CC'TCA_GC
Cuts at: 1001 1007 1001
Size: 6 4667
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3332 4673 2571
Size: 761 1341 2571
Fragments arranged by size:
2571 1341 761
BclI T'GATC_A
Cuts at: 2193 2193
Size: 4673
BglI GCCn_nnn'nGGC
Cuts at: 254 575 254
Size: 321 4352
BglII A'GATC_T
Cuts at: 1091 2188 4225 1091
Size: 1097 2037 1539
Fragments arranged by size:
2037 1539 1097
BlpI GC'TnA_GC
Cuts at: 153 1187 1785 153
Size: 1034 598 3041
Fragments arranged by size:
3041 1034 598
Bme1580I G_kGCm'C
Cuts at: 132 343 1393 2298 2391 3447 132
Size: 211 1050 905 93 1056 1358
Fragments arranged by size:
1358 1056 1050 905 211 93
BmgBI CAC'GTC
Cuts at: 899 899
Size: 4673
BmrI ACTGGGnnnn_n'
Cuts at: 408 1885 1966 2301 4445 408
Size: 1477 81 335 2144 636
Fragments arranged by size:
2144 1477 636 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 850 3040 850
Size: 2190 2483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 544 601 619 646 835 862 1261 1378
Size: 21 57 18 27 189 27 399 117
Cuts at: 1378 1460 1547 1578 1590 523
Size: 82 87 31 12 3606
Fragments arranged by size:
3606 399 189 117 87 82 57 31
27 27 21 18 12
Bpu10I CC'TnA_GC
Cuts at: 613 787 1001 1007 1220 613
Size: 174 214 6 213 4066
Fragments arranged by size:
4066 214 213 174 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 745 760 1129 1195 1849 2728 3220 3518
Size: 589 15 369 66 654 879 492 298
Cuts at: 3518 156
Size: 1311
Fragments arranged by size:
1311 879 654 589 492 369 298 66
15
BsaI GGTCTCn'nnnn_
Cuts at: 433 4377 433
Size: 3944 729
BsaAI yAC'GTr
Cuts at: 2026 2026
Size: 4673
BsaBI GATnn'nnATC
Cuts at: 6 403 2187 4224 6
Size: 397 1784 2037 455
Fragments arranged by size:
2037 1784 455 397
BsaHI Gr'CG_yC
Cuts at: 2104 2352 3054 4550 2104
Size: 248 702 1496 2227
Fragments arranged by size:
2227 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1862 2383 3061 3335 3482 3893 1862
Size: 521 678 274 147 411 2642
Fragments arranged by size:
2642 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 517 547 679 685 709 715 1455 1485 517
Size: 30 132 6 24 6 740 30 3705
Fragments arranged by size:
3705 740 132 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 703 706 727 730 991 1381 1401 1457 703
Size: 3 21 3 261 390 20 56 3919
Fragments arranged by size:
3919 390 261 56 21 20 3 3
BseYI C'CCAG_C
Cuts at: 291 867 1052 2098 2856 3433 3929 291
Size: 576 185 1046 758 577 496 1035
Fragments arranged by size:
1046 1035 758 577 576 496 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1392 1494 1392
Size: 102 4571
BsiEI CG_ry'CG
Cuts at: 104 2215 2261 3469 104
Size: 2111 46 1208 1308
Fragments arranged by size:
2111 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1312 1393 1468 1687 2465 2655 3447 1312
Size: 81 75 219 778 190 792 2538
Fragments arranged by size:
2538 792 778 219 190 81 75
BsmBI CGTCTCn'nnnn_
Cuts at: 520 520
Size: 4673
Bsp1286I G_dGCh'C
Cuts at: 132 332 343 654 1312 1393 1468 1552 1687
Size: 200 11 311 658 81 75 84 135
Cuts at: 1687 2298 2391 2465 2655 2717 3447 132
Size: 611 93 74 190 62 730 1358
Fragments arranged by size:
1358 730 658 611 311 200 190 135
93 84 81 75 74 62 11
BspEI T'CCGG_A
Cuts at: 3893 3893
Size: 4673
BspHI T'CATG_A
Cuts at: 3849 3849
Size: 4673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1723 1937 2239 2620 1723
Size: 214 302 381 3776
Fragments arranged by size:
3776 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2965 3019 4503 4668 50
Size: 2915 54 1484 165 55
Fragments arranged by size:
2915 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2585 2585
Size: 4673
BsrFI r'CCGG_y
Cuts at: 2671 2852 3061 2671
Size: 181 209 4283
Fragments arranged by size:
4283 209 181
BssSI C'ACGA_G
Cuts at: 260 2944 3302 260
Size: 2684 358 1631
Fragments arranged by size:
2684 1631 358
BstAPI GCAn_nnn'nTGC
Cuts at: 543 2050 543
Size: 1507 3166
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstXI CCAn_nnnn'nTGG
Cuts at: 1060 1060
Size: 4673
BstYI r'GATC_y
Cuts at: 1 1091 1698 2165 2188 2523 2769 3781 3867
Size: 1090 607 467 23 335 246 1012 86
Cuts at: 3867 3879 4225 1
Size: 12 346 449
Fragments arranged by size:
1090 1012 607 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 914 914
Size: 4673
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2623 2804 2967 2623
Size: 181 163 4329
Fragments arranged by size:
4329 181 163
BtsI GCAGTG_nn'
Cuts at: 1916 1916
Size: 4673
ClaI AT'CG_AT
Cuts at: 407 407
Size: 4673
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 754 1638 2379 3237 754
Size: 884 741 858 2190
Fragments arranged by size:
2190 884 858 741
EaeI y'GGCC_r
Cuts at: 1259 2258 2432 2823 2850 4195 1259
Size: 999 174 391 27 1345 1737
Fragments arranged by size:
1737 1345 999 391 174 27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4673
EarI CTCTTCn'nnn_
Cuts at: 857 2696 2906 857
Size: 1839 210 2624
Fragments arranged by size:
2624 1839 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2881 3191 3337 4483 2881
Size: 310 146 1146 3071
Fragments arranged by size:
3071 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1685 1685
Size: 4673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 180 198 415 523 544 601 619 646
Size: 54 18 217 108 21 57 18 27
Cuts at: 646 835 862 924 1024 1261 1378 1460 1547
Size: 189 27 62 100 237 117 82 87
Cuts at: 1547 1578 1590 1645 1669 2498 2930 3677 3945
Size: 31 12 55 24 829 432 747 268
Cuts at: 3945 126
Size: 854
Fragments arranged by size:
854 829 747 432 268 237 217 189
117 108 100 87 82 62 57 55
54 31 27 27 24 21 18 18
12
EcoNI CCTnn'n_nnAGG
Cuts at: 763 763
Size: 4673
EcoO109I rG'GnC_Cy
Cuts at: 519 576 1502 1560 1812 519
Size: 57 926 58 252 3380
Fragments arranged by size:
3380 926 252 58 57
EcoRI G'AATT_C
Cuts at: 1677 1677
Size: 4673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4673
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 149 1430 1601 2107 2355 3377 4356 149
Size: 1281 171 506 248 1022 979 466
Fragments arranged by size:
1281 1022 979 506 466 248 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 685 717 1564 1596 2515 2545 2547 2577 685
Size: 32 847 32 919 30 2 30 2781
Fragments arranged by size:
2781 919 847 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1712 1712
Size: 4673
HindIII A'AGCT_T
Cuts at: 1315 1315
Size: 4673
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 782 1559 3344 3528 782
Size: 777 1785 184 1927
Fragments arranged by size:
1927 1785 777 184
MscI TGG'CCA
Cuts at: 1261 2434 1261
Size: 1173 3500
MslI CAynn'nnrTG
Cuts at: 728 854 901 2662 2789 728
Size: 126 47 1761 127 2612
Fragments arranged by size:
2612 1761 127 126 47
MspA1I CmG'CkG
Cuts at: 624 1610 1784 2098 2458 3471 3716 624
Size: 986 174 314 360 1013 245 1581
Fragments arranged by size:
1581 1013 986 360 314 245 174
NaeI GCC'GGC
Cuts at: 2854 2854
Size: 4673
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NcoI C'CATG_G
Cuts at: 914 914
Size: 4673
NgoMIV G'CCGG_C
Cuts at: 2852 2852
Size: 4673
NspI r_CATG'y
Cuts at: 1726 2226 3133 1726
Size: 500 907 3266
Fragments arranged by size:
3266 907 500
PasI CC'CwG_GG
Cuts at: 1498 1498
Size: 4673
PciI A'CATG_T
Cuts at: 3129 3129
Size: 4673
PfoI T'CCnGG_A
Cuts at: 500 521 500
Size: 21 4652
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4641
PpuMI rG'GwC_Cy
Cuts at: 519 1502 1560 519
Size: 983 58 3632
Fragments arranged by size:
3632 983 58
PspOMI G'GGCC_C
Cuts at: 128 128
Size: 4673
PstI C_TGCA'G
Cuts at: 548 1127 1169 1720 2405 548
Size: 579 42 551 685 2816
Fragments arranged by size:
2816 685 579 551 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 624 1610 2098 2458 624
Size: 986 488 360 2839
Fragments arranged by size:
2839 986 488 360
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 1687 1687
Size: 4673
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 857 2696 2906 857
Size: 1839 210 2624
Fragments arranged by size:
2624 1839 210
SbfI CC_TGCA'GG
Cuts at: 1169 1720 1169
Size: 551 4122
ScaI AGT'ACT
Cuts at: 4190 4190
Size: 4673
SfcI C'TryA_G
Cuts at: 33 544 1123 1165 1716 2401 3394 3585 33
Size: 511 579 42 551 685 993 191 1121
Fragments arranged by size:
1121 993 685 579 551 511 191 42
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 171 739 760 1144 1210 1828 2743 3235 3497
Size: 568 21 384 66 618 915 492 262
Cuts at: 3497 171
Size: 1347
Fragments arranged by size:
1347 915 618 568 492 384 262 66
21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4173
StuI AGG'CCT
Cuts at: 598 673 1012 598
Size: 75 339 4259
Fragments arranged by size:
4259 339 75
StyI C'CwwG_G
Cuts at: 914 1226 1807 2161 914
Size: 312 581 354 3426
Fragments arranged by size:
3426 581 354 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2256 2389 2848 3057 2256
Size: 133 459 209 3872
Fragments arranged by size:
3872 459 209 133
TatI w'GTAC_w
Cuts at: 4188 4188
Size: 4673
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 517 549 1455 1487 4190 4222 517
Size: 32 906 32 2703 32 968
Fragments arranged by size:
2703 968 906 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4673
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1269 1542 2168 1269
Size: 273 626 3774
Fragments arranged by size:
3774 626 273
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI