Restriction map for
pF1KB7243
(Circular) (Six-base) MAPSORT of: pF1KB7243.seq Check: 2348 from: 1 to: 4487
pF1KB7243 4487 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 824 1127 1525 824
Size: 303 398 3786
Fragments arranged by size:
3786 398 303
Acc65I G'GTAC_C
Cuts at: 1503 1503
Size: 4487
AclI AA'CG_TT
Cuts at: 4327 4327
Size: 4487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2312 2744 3491 3759 2312
Size: 432 747 268 3040
Fragments arranged by size:
3040 747 432 268
AflIII A'CryG_T
Cuts at: 266 1839 2943 3920 266
Size: 1573 1104 977 833
Fragments arranged by size:
1573 1104 977 833
AgeI A'CCGG_T
Cuts at: 2875 2875
Size: 4487
AleI CACnn'nnGTG
Cuts at: 208 316 751 208
Size: 108 435 3944
Fragments arranged by size:
3944 435 108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 511 511
Size: 4487
AlwNI CAG_nnn'CTG
Cuts at: 291 333 555 864 1792 3359 291
Size: 42 222 309 928 1567 1419
Fragments arranged by size:
1567 1419 928 309 222 42
ApaI G_GGCC'C
Cuts at: 766 964 766
Size: 198 4289
ApaLI G'TGCA_C
Cuts at: 3257 3257
Size: 4487
ApoI r'AATT_y
Cuts at: 1491 3840 4096 1491
Size: 2349 256 1882
Fragments arranged by size:
2349 1882 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4487
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
AvaI C'yCGr_G
Cuts at: 156 1276 1507 156
Size: 1120 231 3136
Fragments arranged by size:
3136 1120 231
BamHI G'GATC_C
Cuts at: 683 1512 683
Size: 829 3658
BanI G'GyrC_C
Cuts at: 583 613 712 1503 1917 2165 2200 583
Size: 30 99 791 414 248 35 2870
Fragments arranged by size:
2870 791 414 248 99 35 30
BanII G_rGCy'C
Cuts at: 259 593 766 964 1501 2531 259
Size: 334 173 198 537 1030 2215
Fragments arranged by size:
2215 1030 537 334 198 173
BbeI G_GCGC'C
Cuts at: 1921 2169 1921
Size: 248 4239
BbsI GAAGACnn'nnnn_
Cuts at: 126 126
Size: 4487
BbvCI CC'TCA_GC
Cuts at: 931 931
Size: 4487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 858 892 858
Size: 34 4453
BciVI GTATCCnnnnn_n'
Cuts at: 2385 3146 4487 2385
Size: 761 1341 2385
Fragments arranged by size:
2385 1341 761
BclI T'GATC_A
Cuts at: 2007 2007
Size: 4487
BglI GCCn_nnn'nGGC
Cuts at: 407 604 806 1435 407
Size: 197 202 629 3459
Fragments arranged by size:
3459 629 202 197
BglII A'GATC_T
Cuts at: 2002 4039 2002
Size: 2037 2450
BlpI GC'TnA_GC
Cuts at: 499 865 1003 1599 499
Size: 366 138 596 3387
Fragments arranged by size:
3387 596 366 138
Bme1580I G_kGCm'C
Cuts at: 717 766 964 2112 2205 3261 717
Size: 49 198 1148 93 1056 1943
Fragments arranged by size:
1943 1148 1056 198 93 49
BmgBI CAC'GTC
Cuts at: 269 269
Size: 4487
BmrI ACTGGGnnnn_n'
Cuts at: 1330 1699 1780 2115 4259 1330
Size: 369 81 335 2144 1558
Fragments arranged by size:
2144 1558 369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 4487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 1067 1071 695
Size: 372 4 4111
Fragments arranged by size:
4111 372 4
Bpu10I CC'TnA_GC
Cuts at: 931 931
Size: 4487
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1663 2542 3034 3332 1663
Size: 879 492 298 2818
Fragments arranged by size:
2818 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 168 569 1255 4191 168
Size: 401 686 2936 464
Fragments arranged by size:
2936 686 464 401
BsaAI yAC'GTr
Cuts at: 1840 1840
Size: 4487
BsaBI GATnn'nnATC
Cuts at: 6 2001 4038 6
Size: 1995 2037 455
Fragments arranged by size:
2037 1995 455
BsaHI Gr'CG_yC
Cuts at: 1394 1918 2166 2868 4364 1394
Size: 524 248 702 1496 1517
Fragments arranged by size:
1517 1496 702 524 248
BsaWI w'CCGG_w
Cuts at: 693 1676 2197 2875 3149 3296 3707 693
Size: 983 521 678 274 147 411 1473
Fragments arranged by size:
1473 983 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 170 200 1040 1070 170
Size: 30 840 30 3587
Fragments arranged by size:
3587 840 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 249 354 433 712 865 1273 178
Size: 71 105 79 279 153 408 3392
Fragments arranged by size:
3392 408 279 153 105 79 71
BseYI C'CCAG_C
Cuts at: 147 446 1270 1912 2670 3247 3743 147
Size: 299 824 642 758 577 496 891
Fragments arranged by size:
891 824 758 642 577 496 299
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 1219 368
Size: 851 3636
BsiEI CG_ry'CG
Cuts at: 104 1358 2029 2075 3283 104
Size: 1254 671 46 1208 1308
Fragments arranged by size:
1308 1254 1208 671 46
BsiHKAI G_wGCw'C
Cuts at: 215 593 784 1501 2279 2469 3261 215
Size: 378 191 717 778 190 792 1441
Fragments arranged by size:
1441 792 778 717 378 191 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1062 1062
Size: 4487
Bsp1286I G_dGCh'C
Cuts at: 215 259 593 717 766 784 964 1501 2112
Size: 44 334 124 49 18 180 537 611
Cuts at: 2112 2205 2279 2469 2531 3261 215
Size: 93 74 190 62 730 1441
Fragments arranged by size:
1441 730 611 537 334 190 180 124
93 74 62 49 44 18
BspEI T'CCGG_A
Cuts at: 693 3707 693
Size: 3014 1473
BspHI T'CATG_A
Cuts at: 3663 3663
Size: 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 120 1537 1751 2053 2434 120
Size: 1417 214 302 381 2173
Fragments arranged by size:
2173 1417 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2779 2833 4317 4482 50
Size: 2729 54 1484 165 55
Fragments arranged by size:
2729 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2399 2399
Size: 4487
BsrFI r'CCGG_y
Cuts at: 560 1365 2485 2666 2875 560
Size: 805 1120 181 209 2172
Fragments arranged by size:
2172 1120 805 209 181
BsrGI T'GTAC_A
Cuts at: 1217 1217
Size: 4487
BssSI C'ACGA_G
Cuts at: 877 1237 2758 3116 877
Size: 360 1521 358 2248
Fragments arranged by size:
2248 1521 360 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1864 1864
Size: 4487
BstBI TT'CG_AA
Cuts at: 2848 2848
Size: 4487
BstXI CCAn_nnnn'nTGG
Cuts at: 471 471
Size: 4487
BstYI r'GATC_y
Cuts at: 1 683 1512 1979 2002 2337 2583 3595 3681
Size: 682 829 467 23 335 246 1012 86
Cuts at: 3681 3693 4039 1
Size: 12 346 449
Fragments arranged by size:
1012 829 682 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1209 1209
Size: 4487
BtgI C'CryG_G
Cuts at: 107 479 569 107
Size: 372 90 4025
Fragments arranged by size:
4025 372 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2437 2618 2781 2437
Size: 181 163 4143
Fragments arranged by size:
4143 181 163
BtsI GCAGTG_nn'
Cuts at: 1730 1730
Size: 4487
DraI TTT'AAA
Cuts at: 1486 1486
Size: 4487
DraIII CAC_nnn'GTG
Cuts at: 982 982
Size: 4487
DrdI GACnn_nn'nnGTC
Cuts at: 820 2193 3051 820
Size: 1373 858 2256
Fragments arranged by size:
2256 1373 858
EaeI y'GGCC_r
Cuts at: 596 1355 1403 2072 2246 2637 2664 4009 596
Size: 759 48 669 174 391 27 1345 1074
Fragments arranged by size:
1345 1074 759 669 391 174 48 27
EagI C'GGCC_G
Cuts at: 1355 2072 1355
Size: 717 3770
EarI CTCTTCn'nnn_
Cuts at: 280 1471 2510 2720 280
Size: 1191 1039 210 2047
Fragments arranged by size:
2047 1191 1039 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2695 3005 3151 4297 2695
Size: 310 146 1146 2885
Fragments arranged by size:
2885 1146 310 146
EcoICRI GAG'CTC
Cuts at: 591 1499 591
Size: 908 3579
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 1067 1071 2312 2744 3491 3759 695
Size: 372 4 1241 432 747 268 1423
Fragments arranged by size:
1423 1241 747 432 372 268 4
EcoNI CCTnn'n_nnAGG
Cuts at: 196 1237 196
Size: 1041 3446
EcoO109I rG'GnC_Cy
Cuts at: 152 330 352 552 731 762 763 1626 152
Size: 178 22 200 179 31 1 863 3013
Fragments arranged by size:
3013 863 200 179 178 31 22 1
EcoRI G'AATT_C
Cuts at: 1491 1491
Size: 4487
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 684 2506 684
Size: 1822 2665
FspI TGC'GCA
Cuts at: 2268 2268
Size: 4487
HaeII r_GCGC'y
Cuts at: 427 1921 2169 3191 4170 427
Size: 1494 248 1022 979 744
Fragments arranged by size:
1494 1022 979 744 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 668 675 700 707 1040 1072 2329
Size: 32 395 7 25 7 333 32 1257
Cuts at: 2329 2359 2361 2391 241
Size: 30 2 30 2337
Fragments arranged by size:
2337 1257 395 333 32 32 30 30
25 7 7 2
HincII GTy'rAC
Cuts at: 636 1526 636
Size: 890 3597
KasI G'GCGC_C
Cuts at: 1917 2165 1917
Size: 248 4239
KpnI G_GTAC'C
Cuts at: 1507 1507
Size: 4487
MluI A'CGCG_T
Cuts at: 3920 3920
Size: 4487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 604 836 3158 3342 604
Size: 232 2322 184 1749
Fragments arranged by size:
2322 1749 232 184
MscI TGG'CCA
Cuts at: 1405 2248 1405
Size: 843 3644
MslI CAynn'nnrTG
Cuts at: 208 316 751 2476 2603 208
Size: 108 435 1725 127 2092
Fragments arranged by size:
2092 1725 435 127 108
MspA1I CmG'CkG
Cuts at: 935 966 1002 1091 1140 1187 1598 1912 2272
Size: 31 36 89 49 47 411 314 360
Cuts at: 2272 3285 3530 935
Size: 1013 245 1892
Fragments arranged by size:
1892 1013 411 360 314 245 89 49
47 36 31
NaeI GCC'GGC
Cuts at: 1367 2668 1367
Size: 1301 3186
NarI GG'CG_CC
Cuts at: 1918 2166 1918
Size: 248 4239
NcoI C'CATG_G
Cuts at: 107 107
Size: 4487
NgoMIV G'CCGG_C
Cuts at: 1365 2666 1365
Size: 1301 3186
NspI r_CATG'y
Cuts at: 1332 1540 2040 2947 1332
Size: 208 500 907 2872
Fragments arranged by size:
2872 907 500 208
PasI CC'CwG_GG
Cuts at: 957 1174 957
Size: 217 4270
PciI A'CATG_T
Cuts at: 2943 2943
Size: 4487
PflMI CCAn_nnn'nTGG
Cuts at: 333 555 333
Size: 222 4265
PfoI T'CCnGG_A
Cuts at: 118 1067 118
Size: 949 3538
PmeI GTTT'AAAC
Cuts at: 1486 1486
Size: 4487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 4455
PpuMI rG'GwC_Cy
Cuts at: 552 552
Size: 4487
PshAI GACnn'nnGTC
Cuts at: 655 655
Size: 4487
PspOMI G'GGCC_C
Cuts at: 762 960 762
Size: 198 4289
PspXI vC'TCGA_Gb
Cuts at: 1276 1276
Size: 4487
PstI C_TGCA'G
Cuts at: 407 1534 2219 407
Size: 1127 685 2675
Fragments arranged by size:
2675 1127 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4487
PvuII CAG'CTG
Cuts at: 1091 1912 2272 1091
Size: 821 360 3306
Fragments arranged by size:
3306 821 360
RsrII CG'GwC_CG
Cuts at: 2682 2682
Size: 4487
SacI G_AGCT'C
Cuts at: 593 1501 593
Size: 908 3579
SalI G'TCGA_C
Cuts at: 1524 1524
Size: 4487
SapI GCTCTTCn'nnn_
Cuts at: 1471 2510 2720 1471
Size: 1039 210 3238
Fragments arranged by size:
3238 1039 210
SbfI CC_TGCA'GG
Cuts at: 407 1534 407
Size: 1127 3360
ScaI AGT'ACT
Cuts at: 664 4004 664
Size: 3340 1147
SfcI C'TryA_G
Cuts at: 33 403 816 1530 2215 3208 3399 33
Size: 370 413 714 685 993 191 1121
Fragments arranged by size:
1121 993 714 685 413 370 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 604 604
Size: 4487
SfoI GGC'GCC
Cuts at: 1919 2167 1919
Size: 248 4239
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
SmaI CCC'GGG
Cuts at: 1509 1509
Size: 4487
SmlI C'TyrA_G
Cuts at: 1276 1642 2557 3049 3311 1276
Size: 366 915 492 262 2452
Fragments arranged by size:
2452 915 492 366 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4487
SphI G_CATG'C
Cuts at: 1540 2040 1540
Size: 500 3987
StuI AGG'CCT
Cuts at: 170 1214 170
Size: 1044 3443
StyI C'CwwG_G
Cuts at: 107 1454 1621 1975 107
Size: 1347 167 354 2619
Fragments arranged by size:
2619 1347 354 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 477 562 700 754 799 1389 2070 2203 2662
Size: 85 138 54 45 590 681 133 459
Cuts at: 2662 2871 477
Size: 209 2093
Fragments arranged by size:
2093 681 590 459 209 138 133 85
54 45
TatI w'GTAC_w
Cuts at: 662 1217 4002 662
Size: 555 2785 1147
Fragments arranged by size:
2785 1147 555
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 170 202 452 484 968 1000 4004 4036 170
Size: 32 250 32 484 32 3004 32 621
Fragments arranged by size:
3004 621 484 250 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1123 2284 1123
Size: 1161 3326
XbaI T'CTAG_A
Cuts at: 1518 1518
Size: 4487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 954 1982 954
Size: 1028 3459
XhoI C'TCGA_G
Cuts at: 1276 1276
Size: 4487
XmaI C'CCGG_G
Cuts at: 1507 1507
Size: 4487
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BfrBI BmtI BsiWI BsmI BssHII BstEII BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PsiI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI