Restriction map for
pF1KB7242
(Circular) (Six-base) MAPSORT of: pF1KB7242.seq Check: 4698 from: 1 to: 3665
pF1KB7242 3665 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 703 703
Size: 3665
Acc65I G'GTAC_C
Cuts at: 681 681
Size: 3665
AclI AA'CG_TT
Cuts at: 3505 3505
Size: 3665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 1490 1922 2669 2937 379
Size: 1111 432 747 268 1107
Fragments arranged by size:
1111 1107 747 432 268
AflIII A'CryG_T
Cuts at: 434 1017 2121 3098 434
Size: 583 1104 977 1001
Fragments arranged by size:
1104 1001 977 583
AgeI A'CCGG_T
Cuts at: 2053 2053
Size: 3665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 294 294
Size: 3665
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 421 453 421
Size: 32 3633
AlwNI CAG_nnn'CTG
Cuts at: 426 970 2537 426
Size: 544 1567 1554
Fragments arranged by size:
1567 1554 544
ApaLI G'TGCA_C
Cuts at: 2435 2435
Size: 3665
ApoI r'AATT_y
Cuts at: 632 669 3018 3274 632
Size: 37 2349 256 1023
Fragments arranged by size:
2349 1023 256 37
AseI AT'TA_AT
Cuts at: 20 20
Size: 3665
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
AvaI C'yCGr_G
Cuts at: 685 685
Size: 3665
BamHI G'GATC_C
Cuts at: 690 690
Size: 3665
BanI G'GyrC_C
Cuts at: 681 1095 1343 1378 681
Size: 414 248 35 2968
Fragments arranged by size:
2968 414 248 35
BanII G_rGCy'C
Cuts at: 617 679 1709 617
Size: 62 1030 2573
Fragments arranged by size:
2573 1030 62
BbeI G_GCGC'C
Cuts at: 1099 1347 1099
Size: 248 3417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 329 363 329
Size: 34 3631
BciVI GTATCCnnnnn_n'
Cuts at: 146 160 1563 2324 3665 146
Size: 14 1403 761 1341 146
Fragments arranged by size:
1403 1341 761 146 14
BclI T'GATC_A
Cuts at: 485 1185 485
Size: 700 2965
BglII A'GATC_T
Cuts at: 1180 3217 1180
Size: 2037 1628
BlpI GC'TnA_GC
Cuts at: 777 777
Size: 3665
Bme1580I G_kGCm'C
Cuts at: 1290 1383 2439 1290
Size: 93 1056 2516
Fragments arranged by size:
2516 1056 93
BmgBI CAC'GTC
Cuts at: 495 495
Size: 3665
BmrI ACTGGGnnnn_n'
Cuts at: 877 958 1293 3437 877
Size: 81 335 2144 1105
Fragments arranged by size:
2144 1105 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 3665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 496 614 426
Size: 70 118 3477
Fragments arranged by size:
3477 118 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1720 2212 2510 841
Size: 879 492 298 1996
Fragments arranged by size:
1996 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3369 3369
Size: 3665
BsaAI yAC'GTr
Cuts at: 1018 1018
Size: 3665
BsaBI GATnn'nnATC
Cuts at: 6 1179 3216 6
Size: 1173 2037 455
Fragments arranged by size:
2037 1173 455
BsaHI Gr'CG_yC
Cuts at: 1096 1344 2046 3542 1096
Size: 248 702 1496 1219
Fragments arranged by size:
1496 1219 702 248
BsaWI w'CCGG_w
Cuts at: 854 1375 2053 2327 2474 2885 854
Size: 521 678 274 147 411 1634
Fragments arranged by size:
1634 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 424 454 424
Size: 30 3635
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 374 374
Size: 3665
BseYI C'CCAG_C
Cuts at: 610 1090 1848 2425 2921 610
Size: 480 758 577 496 1354
Fragments arranged by size:
1354 758 577 496 480
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 665
Size: 3665
BsiEI CG_ry'CG
Cuts at: 104 1207 1253 2461 104
Size: 1103 46 1208 1308
Fragments arranged by size:
1308 1208 1103 46
BsiHKAI G_wGCw'C
Cuts at: 335 679 1457 1647 2439 335
Size: 344 778 190 792 1561
Fragments arranged by size:
1561 792 778 344 190
Bsp1286I G_dGCh'C
Cuts at: 335 617 679 1290 1383 1457 1647 1709 2439
Size: 282 62 611 93 74 190 62 730
Cuts at: 2439 335
Size: 1561
Fragments arranged by size:
1561 730 611 282 190 93 74 62
62
BspEI T'CCGG_A
Cuts at: 2885 2885
Size: 3665
BspHI T'CATG_A
Cuts at: 2841 2841
Size: 3665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 715 929 1231 1612 715
Size: 214 302 381 2768
Fragments arranged by size:
2768 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1957 2011 3495 3660 50
Size: 1907 54 1484 165 55
Fragments arranged by size:
1907 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 216 317 1577 216
Size: 101 1260 2304
Fragments arranged by size:
2304 1260 101
BsrFI r'CCGG_y
Cuts at: 1663 1844 2053 1663
Size: 181 209 3275
Fragments arranged by size:
3275 209 181
BssSI C'ACGA_G
Cuts at: 1936 2294 1936
Size: 358 3307
BstAPI GCAn_nnn'nTGC
Cuts at: 1042 1042
Size: 3665
BstBI TT'CG_AA
Cuts at: 2026 2026
Size: 3665
BstEII G'GTnAC_C
Cuts at: 515 515
Size: 3665
BstYI r'GATC_y
Cuts at: 1 690 1157 1180 1515 1761 2773 2859 2871
Size: 689 467 23 335 246 1012 86 12
Cuts at: 2871 3217 1
Size: 346 449
Fragments arranged by size:
1012 689 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1615 1796 1959 1615
Size: 181 163 3321
Fragments arranged by size:
3321 181 163
BtsI GCAGTG_nn'
Cuts at: 908 908
Size: 3665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 186 221 279 314 186
Size: 35 58 35 3537
Fragments arranged by size:
3537 58 35 35
DraI TTT'AAA
Cuts at: 664 664
Size: 3665
DrdI GACnn_nn'nnGTC
Cuts at: 1371 2229 1371
Size: 858 2807
EaeI y'GGCC_r
Cuts at: 1250 1424 1815 1842 3187 1250
Size: 174 391 27 1345 1728
Fragments arranged by size:
1728 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1250 1250
Size: 3665
EarI CTCTTCn'nnn_
Cuts at: 288 336 1688 1898 288
Size: 48 1352 210 2055
Fragments arranged by size:
2055 1352 210 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1873 2183 2329 3475 1873
Size: 310 146 1146 2063
Fragments arranged by size:
2063 1146 310 146
EcoICRI GAG'CTC
Cuts at: 677 677
Size: 3665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 426 496 614 1490 1922 2669 2937 379
Size: 47 70 118 876 432 747 268 1107
Fragments arranged by size:
1107 876 747 432 268 118 70 47
EcoO109I rG'GnC_Cy
Cuts at: 804 804
Size: 3665
EcoRI G'AATT_C
Cuts at: 669 669
Size: 3665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1684 1684
Size: 3665
FspI TGC'GCA
Cuts at: 1446 1446
Size: 3665
HaeII r_GCGC'y
Cuts at: 1099 1347 2369 3348 1099
Size: 248 1022 979 1416
Fragments arranged by size:
1416 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 144 176 354 386 1507 1537 1539 1569 144
Size: 32 178 32 1121 30 2 30 2240
Fragments arranged by size:
2240 1121 178 32 32 30 30 2
HincII GTy'rAC
Cuts at: 704 704
Size: 3665
KasI G'GCGC_C
Cuts at: 1095 1343 1095
Size: 248 3417
KpnI G_GTAC'C
Cuts at: 685 685
Size: 3665
MfeI C'AATT_G
Cuts at: 230 230
Size: 3665
MluI A'CGCG_T
Cuts at: 3098 3098
Size: 3665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2520 2336
Size: 184 3481
MscI TGG'CCA
Cuts at: 1426 1426
Size: 3665
MslI CAynn'nnrTG
Cuts at: 1654 1781 1654
Size: 127 3538
MspA1I CmG'CkG
Cuts at: 776 1090 1450 2463 2708 776
Size: 314 360 1013 245 1733
Fragments arranged by size:
1733 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1846 1846
Size: 3665
NarI GG'CG_CC
Cuts at: 1096 1344 1096
Size: 248 3417
NgoMIV G'CCGG_C
Cuts at: 1844 1844
Size: 3665
NspI r_CATG'y
Cuts at: 438 718 1218 2125 438
Size: 280 500 907 1978
Fragments arranged by size:
1978 907 500 280
PciI A'CATG_T
Cuts at: 434 2121 434
Size: 1687 1978
PmeI GTTT'AAAC
Cuts at: 664 664
Size: 3665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 3633
PstI C_TGCA'G
Cuts at: 712 1397 712
Size: 685 2980
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3665
PvuII CAG'CTG
Cuts at: 1090 1450 1090
Size: 360 3305
RsrII CG'GwC_CG
Cuts at: 1860 1860
Size: 3665
SacI G_AGCT'C
Cuts at: 679 679
Size: 3665
SalI G'TCGA_C
Cuts at: 702 702
Size: 3665
SapI GCTCTTCn'nnn_
Cuts at: 288 336 1688 1898 288
Size: 48 1352 210 2055
Fragments arranged by size:
2055 1352 210 48
SbfI CC_TGCA'GG
Cuts at: 712 712
Size: 3665
ScaI AGT'ACT
Cuts at: 3182 3182
Size: 3665
SfcI C'TryA_G
Cuts at: 33 708 1393 2386 2577 33
Size: 675 685 993 191 1121
Fragments arranged by size:
1121 993 685 675 191
SfoI GGC'GCC
Cuts at: 1097 1345 1097
Size: 248 3417
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
SmaI CCC'GGG
Cuts at: 687 687
Size: 3665
SmlI C'TyrA_G
Cuts at: 820 1735 2227 2489 820
Size: 915 492 262 1996
Fragments arranged by size:
1996 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3665
SphI G_CATG'C
Cuts at: 718 1218 718
Size: 500 3165
StyI C'CwwG_G
Cuts at: 799 1153 799
Size: 354 3311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 534 1248 1381 1840 2049 534
Size: 714 133 459 209 2150
Fragments arranged by size:
2150 714 459 209 133
TatI w'GTAC_w
Cuts at: 464 3180 464
Size: 2716 949
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 3633
Tth111I GACn'n_nGTC
Cuts at: 1462 1462
Size: 3665
XbaI T'CTAG_A
Cuts at: 696 696
Size: 3665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1160 1160
Size: 3665
XmaI C'CCGG_G
Cuts at: 685 685
Size: 3665
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
BtgI ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI