Restriction map for
pF1KB7240
(Circular) (Six-base) MAPSORT of: pF1KB7240.seq Check: 5736 from: 1 to: 4046
pF1KB7240 4046 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 278 278
Size: 4046
AccI GT'mk_AC
Cuts at: 1084 1084
Size: 4046
Acc65I G'GTAC_C
Cuts at: 1062 1062
Size: 4046
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 1030 1871 2303 3050 3318 804
Size: 226 841 432 747 268 1532
Fragments arranged by size:
1532 841 747 432 268 226
AflIII A'CryG_T
Cuts at: 446 839 1398 2502 3479 446
Size: 393 559 1104 977 1013
Fragments arranged by size:
1104 1013 977 559 393
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AleI CACnn'nnGTG
Cuts at: 223 223
Size: 4046
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 367 399 367
Size: 32 4014
AlwNI CAG_nnn'CTG
Cuts at: 136 1351 2918 136
Size: 1215 1567 1264
Fragments arranged by size:
1567 1264 1215
ApaLI G'TGCA_C
Cuts at: 2816 2816
Size: 4046
ApoI r'AATT_y
Cuts at: 616 1050 3399 3655 616
Size: 434 2349 256 1007
Fragments arranged by size:
2349 1007 434 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 1066 1066
Size: 4046
BamHI G'GATC_C
Cuts at: 1071 1071
Size: 4046
BanI G'GyrC_C
Cuts at: 1062 1476 1724 1759 1062
Size: 414 248 35 3349
Fragments arranged by size:
3349 414 248 35
BanII G_rGCy'C
Cuts at: 158 176 1060 2090 158
Size: 18 884 1030 2114
Fragments arranged by size:
2114 1030 884 18
BbeI G_GCGC'C
Cuts at: 1480 1728 1480
Size: 248 3798
BbvCI CC'TCA_GC
Cuts at: 295 295
Size: 4046
BciVI GTATCCnnnnn_n'
Cuts at: 936 1944 2705 4046 936
Size: 1008 761 1341 936
Fragments arranged by size:
1341 1008 936 761
BclI T'GATC_A
Cuts at: 1566 1566
Size: 4046
BfrBI ATG'CAT
Cuts at: 398 398
Size: 4046
BglII A'GATC_T
Cuts at: 1561 3598 1561
Size: 2037 2009
BlpI GC'TnA_GC
Cuts at: 1158 1158
Size: 4046
Bme1580I G_kGCm'C
Cuts at: 956 1671 1764 2820 956
Size: 715 93 1056 2182
Fragments arranged by size:
2182 1056 715 93
BmrI ACTGGGnnnn_n'
Cuts at: 1258 1339 1674 3818 1258
Size: 81 335 2144 1486
Fragments arranged by size:
2144 1486 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 160
Size: 4046
Bpu10I CC'TnA_GC
Cuts at: 295 295
Size: 4046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 2101 2593 2891 1222
Size: 879 492 298 2377
Fragments arranged by size:
2377 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3750 3750
Size: 4046
BsaAI yAC'GTr
Cuts at: 1399 1399
Size: 4046
BsaBI GATnn'nnATC
Cuts at: 6 1560 3597 6
Size: 1554 2037 455
Fragments arranged by size:
2037 1554 455
BsaHI Gr'CG_yC
Cuts at: 1477 1725 2427 3923 1477
Size: 248 702 1496 1600
Fragments arranged by size:
1600 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1235 1756 2434 2708 2855 3266 1235
Size: 521 678 274 147 411 2015
Fragments arranged by size:
2015 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 367 397 778 808 367
Size: 30 381 30 3605
Fragments arranged by size:
3605 381 30 30
BseYI C'CCAG_C
Cuts at: 151 736 1471 2229 2806 3302 151
Size: 585 735 758 577 496 895
Fragments arranged by size:
895 758 735 585 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4046
BsiEI CG_ry'CG
Cuts at: 104 1588 1634 2842 104
Size: 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 176 1060 1838 2028 2820 176
Size: 884 778 190 792 1402
Fragments arranged by size:
1402 884 792 778 190
BsmI GAATG_Cn'
Cuts at: 400 874 400
Size: 474 3572
Bsp1286I G_dGCh'C
Cuts at: 158 176 956 1060 1671 1764 1838 2028 2090
Size: 18 780 104 611 93 74 190 62
Cuts at: 2090 2820 158
Size: 730 1384
Fragments arranged by size:
1384 780 730 611 190 104 93 74
62 18
BspEI T'CCGG_A
Cuts at: 3266 3266
Size: 4046
BspHI T'CATG_A
Cuts at: 3222 3222
Size: 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 797 1096 1310 1612 1993 278
Size: 519 299 214 302 381 2331
Fragments arranged by size:
2331 519 381 302 299 214
BsrBI CCG'CTC
Cuts at: 50 2338 2392 3876 4041 50
Size: 2288 54 1484 165 55
Fragments arranged by size:
2288 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 464 1958 464
Size: 1494 2552
BsrFI r'CCGG_y
Cuts at: 2044 2225 2434 2044
Size: 181 209 3656
Fragments arranged by size:
3656 209 181
BsrGI T'GTAC_A
Cuts at: 500 500
Size: 4046
BssSI C'ACGA_G
Cuts at: 2317 2675 2317
Size: 358 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 1423 1423
Size: 4046
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstYI r'GATC_y
Cuts at: 1 865 1071 1538 1561 1896 2142 3154 3240
Size: 864 206 467 23 335 246 1012 86
Cuts at: 3240 3252 3598 1
Size: 12 346 449
Fragments arranged by size:
1012 864 467 449 346 335 246 206
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 982 982
Size: 4046
BtgI C'CryG_G
Cuts at: 908 908
Size: 4046
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 1289 1289
Size: 4046
DraI TTT'AAA
Cuts at: 1045 1045
Size: 4046
DrdI GACnn_nn'nnGTC
Cuts at: 1752 2610 1752
Size: 858 3188
EaeI y'GGCC_r
Cuts at: 479 1631 1805 2196 2223 3568 479
Size: 1152 174 391 27 1345 957
Fragments arranged by size:
1345 1152 957 391 174 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 193 2069 2279 193
Size: 1876 210 1960
Fragments arranged by size:
1960 1876 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2254 2564 2710 3856 2254
Size: 310 146 1146 2444
Fragments arranged by size:
2444 1146 310 146
EcoICRI GAG'CTC
Cuts at: 174 1058 174
Size: 884 3162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 804 1030 1871 2303 3050 3318 160
Size: 644 226 841 432 747 268 888
Fragments arranged by size:
888 841 747 644 432 268 226
EcoO109I rG'GnC_Cy
Cuts at: 1185 1185
Size: 4046
EcoRI G'AATT_C
Cuts at: 1050 1050
Size: 4046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 546 2065 546
Size: 1519 2527
FspI TGC'GCA
Cuts at: 1827 1827
Size: 4046
HaeII r_GCGC'y
Cuts at: 1480 1728 2750 3729 1480
Size: 248 1022 979 1797
Fragments arranged by size:
1797 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 793 825 1888 1918 1920 1950 793
Size: 32 1063 30 2 30 2889
Fragments arranged by size:
2889 1063 32 30 30 2
HincII GTy'rAC
Cuts at: 690 1085 690
Size: 395 3651
KasI G'GCGC_C
Cuts at: 1476 1724 1476
Size: 248 3798
KpnI G_GTAC'C
Cuts at: 1066 1066
Size: 4046
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2717 2901 2717
Size: 184 3862
MscI TGG'CCA
Cuts at: 481 1807 481
Size: 1326 2720
MslI CAynn'nnrTG
Cuts at: 223 280 2035 2162 223
Size: 57 1755 127 2107
Fragments arranged by size:
2107 1755 127 57
MspA1I CmG'CkG
Cuts at: 180 1157 1471 1831 2844 3089 180
Size: 977 314 360 1013 245 1137
Fragments arranged by size:
1137 1013 977 360 314 245
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 4046
NarI GG'CG_CC
Cuts at: 1477 1725 1477
Size: 248 3798
NcoI C'CATG_G
Cuts at: 908 908
Size: 4046
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 4046
NsiI A_TGCA'T
Cuts at: 400 400
Size: 4046
NspI r_CATG'y
Cuts at: 450 843 1099 1599 2506 450
Size: 393 256 500 907 1990
Fragments arranged by size:
1990 907 500 393 256
PciI A'CATG_T
Cuts at: 446 839 2502 446
Size: 393 1663 1990
Fragments arranged by size:
1990 1663 393
PflMI CCAn_nnn'nTGG
Cuts at: 843 843
Size: 4046
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 367 399 3185 3217 367
Size: 32 2786 32 1196
Fragments arranged by size:
2786 1196 32 32
PstI C_TGCA'G
Cuts at: 873 1093 1778 873
Size: 220 685 3141
Fragments arranged by size:
3141 685 220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 180 1471 1831 180
Size: 1291 360 2395
Fragments arranged by size:
2395 1291 360
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 176 1060 176
Size: 884 3162
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 1093 1093
Size: 4046
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SfcI C'TryA_G
Cuts at: 33 486 869 1021 1089 1774 2767 2958 33
Size: 453 383 152 68 685 993 191 1121
Fragments arranged by size:
1121 993 685 453 383 191 152 68
SfoI GGC'GCC
Cuts at: 1478 1726 1478
Size: 248 3798
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 1201 2116 2608 2870 1201
Size: 915 492 262 2377
Fragments arranged by size:
2377 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
StuI AGG'CCT
Cuts at: 563 563
Size: 4046
StyI C'CwwG_G
Cuts at: 908 1180 1534 908
Size: 272 354 3420
Fragments arranged by size:
3420 354 272
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1629 1762 2221 2430 1629
Size: 133 459 209 3245
Fragments arranged by size:
3245 459 209 133
TatI w'GTAC_w
Cuts at: 284 500 881 3561 284
Size: 216 381 2680 769
Fragments arranged by size:
2680 769 381 216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 197 229 778 810 3563 3595 197
Size: 32 549 32 2753 32 648
Fragments arranged by size:
2753 648 549 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1843 1843
Size: 4046
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1541 1541
Size: 4046
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BbsI BcgI BglI BmgBI BmtI BseRI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI