Restriction map for
pF1KB7237
(Circular) (Six-base) MAPSORT of: pF1KB7237.seq Check: 6117 from: 1 to: 4556
pF1KB7237 4556 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 313 503 1594 313
Size: 190 1091 3275
Fragments arranged by size:
3275 1091 190
Acc65I G'GTAC_C
Cuts at: 1572 1572
Size: 4556
AclI AA'CG_TT
Cuts at: 4396 4396
Size: 4556
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 433 2381 2813 3560 3828 252
Size: 181 1948 432 747 268 980
Fragments arranged by size:
1948 980 747 432 268 181
AflIII A'CryG_T
Cuts at: 1531 1908 3012 3989 1531
Size: 377 1104 977 2098
Fragments arranged by size:
2098 1104 977 377
AgeI A'CCGG_T
Cuts at: 2944 2944
Size: 4556
AlwNI CAG_nnn'CTG
Cuts at: 1861 3428 1861
Size: 1567 2989
ApaLI G'TGCA_C
Cuts at: 583 3326 583
Size: 2743 1813
ApoI r'AATT_y
Cuts at: 238 905 1560 3909 4165 238
Size: 667 655 2349 256 629
Fragments arranged by size:
2349 667 655 629 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4556
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
AvaI C'yCGr_G
Cuts at: 1576 1576
Size: 4556
BamHI G'GATC_C
Cuts at: 1581 1581
Size: 4556
BanI G'GyrC_C
Cuts at: 1572 1986 2234 2269 1572
Size: 414 248 35 3859
Fragments arranged by size:
3859 414 248 35
BanII G_rGCy'C
Cuts at: 657 833 1393 1570 2600 657
Size: 176 560 177 1030 2613
Fragments arranged by size:
2613 1030 560 177 176
BbeI G_GCGC'C
Cuts at: 1990 2238 1990
Size: 248 4308
BbsI GAAGACnn'nnnn_
Cuts at: 1128 1128
Size: 4556
BciVI GTATCCnnnnn_n'
Cuts at: 409 1143 2454 3215 4556 409
Size: 734 1311 761 1341 409
Fragments arranged by size:
1341 1311 761 734 409
BclI T'GATC_A
Cuts at: 2076 2076
Size: 4556
BglII A'GATC_T
Cuts at: 2071 4108 2071
Size: 2037 2519
BlpI GC'TnA_GC
Cuts at: 338 1668 338
Size: 1330 3226
Bme1580I G_kGCm'C
Cuts at: 587 2181 2274 3330 587
Size: 1594 93 1056 1813
Fragments arranged by size:
1813 1594 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1768 1849 2184 4328 1768
Size: 81 335 2144 1996
Fragments arranged by size:
2144 1996 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2923 2923
Size: 4556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 723 528
Size: 195 4361
Bpu10I CC'TnA_GC
Cuts at: 648 648
Size: 4556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1732 2611 3103 3401 1732
Size: 879 492 298 2887
Fragments arranged by size:
2887 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 289 4260 289
Size: 3971 585
BsaAI yAC'GTr
Cuts at: 1909 1909
Size: 4556
BsaBI GATnn'nnATC
Cuts at: 6 851 2070 4107 6
Size: 845 1219 2037 455
Fragments arranged by size:
2037 1219 845 455
BsaHI Gr'CG_yC
Cuts at: 1987 2235 2937 4433 1987
Size: 248 702 1496 2110
Fragments arranged by size:
2110 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1745 2266 2944 3218 3365 3776 1745
Size: 521 678 274 147 411 2525
Fragments arranged by size:
2525 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 501 531 501
Size: 30 4526
BseYI C'CCAG_C
Cuts at: 724 1068 1981 2739 3316 3812 724
Size: 344 913 758 577 496 1468
Fragments arranged by size:
1468 913 758 577 496 344
BsiEI CG_ry'CG
Cuts at: 104 2098 2144 3352 104
Size: 1994 46 1208 1308
Fragments arranged by size:
1994 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 156 587 1393 1570 2348 2538 3330 156
Size: 431 806 177 778 190 792 1382
Fragments arranged by size:
1382 806 792 778 431 190 177
BsmI GAATG_Cn'
Cuts at: 821 821
Size: 4556
Bsp1286I G_dGCh'C
Cuts at: 156 587 657 833 1393 1570 2181 2274 2348
Size: 431 70 176 560 177 611 93 74
Cuts at: 2348 2538 2600 3330 156
Size: 190 62 730 1382
Fragments arranged by size:
1382 730 611 560 431 190 177 176
93 74 70 62
BspEI T'CCGG_A
Cuts at: 3776 3776
Size: 4556
BspHI T'CATG_A
Cuts at: 3732 3732
Size: 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1469 1606 1820 2122 2503 1469
Size: 137 214 302 381 3522
Fragments arranged by size:
3522 381 302 214 137
BsrBI CCG'CTC
Cuts at: 50 2848 2902 4386 4551 50
Size: 2798 54 1484 165 55
Fragments arranged by size:
2798 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2468 2468
Size: 4556
BsrFI r'CCGG_y
Cuts at: 2554 2735 2944 2554
Size: 181 209 4166
Fragments arranged by size:
4166 209 181
BsrGI T'GTAC_A
Cuts at: 1534 1534
Size: 4556
BssSI C'ACGA_G
Cuts at: 2827 3185 2827
Size: 358 4198
BstAPI GCAn_nnn'nTGC
Cuts at: 1933 1933
Size: 4556
BstBI TT'CG_AA
Cuts at: 2917 2917
Size: 4556
BstYI r'GATC_y
Cuts at: 1 1424 1581 2048 2071 2406 2652 3664 3750
Size: 1423 157 467 23 335 246 1012 86
Cuts at: 3750 3762 4108 1
Size: 12 346 449
Fragments arranged by size:
1423 1012 467 449 346 335 246 157
86 23 12
BstZ17I GTA'TAC
Cuts at: 314 314
Size: 4556
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2506 2687 2850 2506
Size: 181 163 4212
Fragments arranged by size:
4212 181 163
BtsI GCAGTG_nn'
Cuts at: 1408 1799 1408
Size: 391 4165
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 767 802 767
Size: 35 4521
DraI TTT'AAA
Cuts at: 1092 1103 1242 1555 1092
Size: 11 139 313 4093
Fragments arranged by size:
4093 313 139 11
DrdI GACnn_nn'nnGTC
Cuts at: 2262 3120 2262
Size: 858 3698
EaeI y'GGCC_r
Cuts at: 2141 2315 2706 2733 4078 2141
Size: 174 391 27 1345 2619
Fragments arranged by size:
2619 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2141 2141
Size: 4556
EarI CTCTTCn'nnn_
Cuts at: 1265 2579 2789 1265
Size: 1314 210 3032
Fragments arranged by size:
3032 1314 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2764 3074 3220 4366 2764
Size: 310 146 1146 2954
Fragments arranged by size:
2954 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1391 1568 1391
Size: 177 4379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 433 528 723 2381 2813 3560 3828 252
Size: 181 95 195 1658 432 747 268 980
Fragments arranged by size:
1658 980 747 432 268 195 181 95
EcoO109I rG'GnC_Cy
Cuts at: 1695 1695
Size: 4556
EcoRI G'AATT_C
Cuts at: 238 905 1560 238
Size: 667 655 3234
Fragments arranged by size:
3234 667 655
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 282 1038 1534 2575 282
Size: 756 496 1041 2263
Fragments arranged by size:
2263 1041 756 496
FspI TGC'GCA
Cuts at: 2337 2337
Size: 4556
HaeII r_GCGC'y
Cuts at: 1990 2238 3260 4239 1990
Size: 248 1022 979 2307
Fragments arranged by size:
2307 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1317 1349 1380 1412 2398 2428 2430 2460 1317
Size: 32 31 32 986 30 2 30 3413
Fragments arranged by size:
3413 986 32 32 31 30 30 2
HincII GTy'rAC
Cuts at: 694 1595 694
Size: 901 3655
KasI G'GCGC_C
Cuts at: 1986 2234 1986
Size: 248 4308
KpnI G_GTAC'C
Cuts at: 1576 1576
Size: 4556
MluI A'CGCG_T
Cuts at: 3989 3989
Size: 4556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 654 3227 3411 654
Size: 2573 184 1799
Fragments arranged by size:
2573 1799 184
MscI TGG'CCA
Cuts at: 2317 2317
Size: 4556
MslI CAynn'nnrTG
Cuts at: 304 640 662 2545 2672 304
Size: 336 22 1883 127 2188
Fragments arranged by size:
2188 1883 336 127 22
MspA1I CmG'CkG
Cuts at: 1667 1981 2341 3354 3599 1667
Size: 314 360 1013 245 2624
Fragments arranged by size:
2624 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2737 2737
Size: 4556
NarI GG'CG_CC
Cuts at: 1987 2235 1987
Size: 248 4308
NgoMIV G'CCGG_C
Cuts at: 2735 2735
Size: 4556
NspI r_CATG'y
Cuts at: 1415 1535 1609 2109 3016 1415
Size: 120 74 500 907 2955
Fragments arranged by size:
2955 907 500 120 74
PciI A'CATG_T
Cuts at: 1531 3012 1531
Size: 1481 3075
PmeI GTTT'AAAC
Cuts at: 1555 1555
Size: 4556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4524
PsiI TTA'TAA
Cuts at: 536 1307 1343 536
Size: 771 36 3749
Fragments arranged by size:
3749 771 36
PstI C_TGCA'G
Cuts at: 1403 1603 2288 1403
Size: 200 685 3671
Fragments arranged by size:
3671 685 200
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4556
PvuII CAG'CTG
Cuts at: 1981 2341 1981
Size: 360 4196
RsrII CG'GwC_CG
Cuts at: 2751 2751
Size: 4556
SacI G_AGCT'C
Cuts at: 1393 1570 1393
Size: 177 4379
SalI G'TCGA_C
Cuts at: 1593 1593
Size: 4556
SapI GCTCTTCn'nnn_
Cuts at: 1265 2579 2789 1265
Size: 1314 210 3032
Fragments arranged by size:
3032 1314 210
SbfI CC_TGCA'GG
Cuts at: 1603 1603
Size: 4556
ScaI AGT'ACT
Cuts at: 4073 4073
Size: 4556
SfcI C'TryA_G
Cuts at: 33 1399 1599 2284 3277 3468 33
Size: 1366 200 685 993 191 1121
Fragments arranged by size:
1366 1121 993 685 200 191
SfoI GGC'GCC
Cuts at: 1988 2236 1988
Size: 248 4308
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
SmaI CCC'GGG
Cuts at: 1578 1578
Size: 4556
SmlI C'TyrA_G
Cuts at: 1711 2626 3118 3380 1711
Size: 915 492 262 2887
Fragments arranged by size:
2887 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4556
SphI G_CATG'C
Cuts at: 1609 2109 1609
Size: 500 4056
SspI AAT'ATT
Cuts at: 1359 1488 1359
Size: 129 4427
StyI C'CwwG_G
Cuts at: 730 833 1276 1690 2044 730
Size: 103 443 414 354 3242
Fragments arranged by size:
3242 443 414 354 103
SwaI ATTT'AAAT
Cuts at: 1242 1242
Size: 4556
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 189 2139 2272 2731 2940 189
Size: 1950 133 459 209 1805
Fragments arranged by size:
1950 1805 459 209 133
TatI w'GTAC_w
Cuts at: 1491 1534 4071 1491
Size: 43 2537 1976
Fragments arranged by size:
2537 1976 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 501 533 4073 4105 501
Size: 32 3540 32 952
Fragments arranged by size:
3540 952 32 32
Tth111I GACn'n_nGTC
Cuts at: 2353 2353
Size: 4556
XbaI T'CTAG_A
Cuts at: 1587 1587
Size: 4556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1143 2051 1143
Size: 908 3648
XmaI C'CCGG_G
Cuts at: 1576 1576
Size: 4556
XmnI GAAnn'nnTTC
Cuts at: 1514 1514
Size: 4556
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BstZ17I BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BseRI BsgI BsiWI BsmBI BssHII BstEII
BstXI Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI XhoI ZraI