Restriction map for
pF1KB7236
(Circular) (Six-base) MAPSORT of: pF1KB7236.seq Check: 7179 from: 1 to: 4046
pF1KB7236 4046 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1084 1084
Size: 4046
Acc65I G'GTAC_C
Cuts at: 1062 1062
Size: 4046
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1871 2303 3050 3318 1871
Size: 432 747 268 2599
Fragments arranged by size:
2599 747 432 268
AfeI AGC'GCT
Cuts at: 202 202
Size: 4046
AflIII A'CryG_T
Cuts at: 305 1398 2502 3479 305
Size: 1093 1104 977 872
Fragments arranged by size:
1104 1093 977 872
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AlwNI CAG_nnn'CTG
Cuts at: 262 627 730 1351 2918 262
Size: 365 103 621 1567 1390
Fragments arranged by size:
1567 1390 621 365 103
ApaI G_GGCC'C
Cuts at: 401 401
Size: 4046
ApaLI G'TGCA_C
Cuts at: 2816 2816
Size: 4046
ApoI r'AATT_y
Cuts at: 1050 3399 3655 1050
Size: 2349 256 1441
Fragments arranged by size:
2349 1441 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 1066 1066
Size: 4046
BamHI G'GATC_C
Cuts at: 1071 1071
Size: 4046
BanI G'GyrC_C
Cuts at: 1062 1476 1724 1759 1062
Size: 414 248 35 3349
Fragments arranged by size:
3349 414 248 35
BanII G_rGCy'C
Cuts at: 401 413 1060 2090 401
Size: 12 647 1030 2357
Fragments arranged by size:
2357 1030 647 12
BbeI G_GCGC'C
Cuts at: 1480 1728 1480
Size: 248 3798
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 553 587 553
Size: 34 4012
BciVI GTATCCnnnnn_n'
Cuts at: 355 1944 2705 4046 355
Size: 1589 761 1341 355
Fragments arranged by size:
1589 1341 761 355
BclI T'GATC_A
Cuts at: 524 1566 524
Size: 1042 3004
BglII A'GATC_T
Cuts at: 1561 3598 1561
Size: 2037 2009
BlpI GC'TnA_GC
Cuts at: 412 1158 412
Size: 746 3300
Bme1580I G_kGCm'C
Cuts at: 401 1671 1764 2820 401
Size: 1270 93 1056 1627
Fragments arranged by size:
1627 1270 1056 93
BmgBI CAC'GTC
Cuts at: 306 363 819 306
Size: 57 456 3533
Fragments arranged by size:
3533 456 57
BmrI ACTGGGnnnn_n'
Cuts at: 324 1258 1339 1674 3818 324
Size: 934 81 335 2144 552
Fragments arranged by size:
2144 934 552 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 424 613 762 271
Size: 153 189 149 3555
Fragments arranged by size:
3555 189 153 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 2101 2593 2891 1222
Size: 879 492 298 2377
Fragments arranged by size:
2377 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 733 3750 733
Size: 3017 1029
BsaAI yAC'GTr
Cuts at: 1399 1399
Size: 4046
BsaBI GATnn'nnATC
Cuts at: 6 1560 3597 6
Size: 1554 2037 455
Fragments arranged by size:
2037 1554 455
BsaHI Gr'CG_yC
Cuts at: 1477 1725 2427 3923 1477
Size: 248 702 1496 1600
Fragments arranged by size:
1600 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1235 1756 2434 2708 2855 3266 1235
Size: 521 678 274 147 411 2015
Fragments arranged by size:
2015 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 513 949 970 513
Size: 436 21 3589
Fragments arranged by size:
3589 436 21
BseYI C'CCAG_C
Cuts at: 262 296 771 1471 2229 2806 3302 262
Size: 34 475 700 758 577 496 1006
Fragments arranged by size:
1006 758 700 577 496 475 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 330 730 101
Size: 229 400 3417
Fragments arranged by size:
3417 400 229
BsiEI CG_ry'CG
Cuts at: 104 1588 1634 2842 104
Size: 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1060 1838 2028 2820 1060
Size: 778 190 792 2286
Fragments arranged by size:
2286 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 472 472
Size: 4046
Bsp1286I G_dGCh'C
Cuts at: 401 413 1060 1671 1764 1838 2028 2090 2820
Size: 12 647 611 93 74 190 62 730
Cuts at: 2820 401
Size: 1627
Fragments arranged by size:
1627 730 647 611 190 93 74 62
12
BspEI T'CCGG_A
Cuts at: 3266 3266
Size: 4046
BspHI T'CATG_A
Cuts at: 3222 3222
Size: 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 693 1096 1310 1612 1993 693
Size: 403 214 302 381 2746
Fragments arranged by size:
2746 403 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2338 2392 3876 4041 50
Size: 2288 54 1484 165 55
Fragments arranged by size:
2288 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1958 1958
Size: 4046
BsrFI r'CCGG_y
Cuts at: 2044 2225 2434 2044
Size: 181 209 3656
Fragments arranged by size:
3656 209 181
BssSI C'ACGA_G
Cuts at: 2317 2675 2317
Size: 358 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 1423 1423
Size: 4046
BstBI TT'CG_AA
Cuts at: 605 2407 605
Size: 1802 2244
BstEII G'GTnAC_C
Cuts at: 672 672
Size: 4046
BstYI r'GATC_y
Cuts at: 1 1071 1538 1561 1896 2142 3154 3240 3252
Size: 1070 467 23 335 246 1012 86 12
Cuts at: 3252 3598 1
Size: 346 449
Fragments arranged by size:
1070 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 290 668 797 914 107
Size: 183 378 129 117 3239
Fragments arranged by size:
3239 378 183 129 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 179 318 537 1289 179
Size: 139 219 752 2936
Fragments arranged by size:
2936 752 219 139
DraI TTT'AAA
Cuts at: 1045 1045
Size: 4046
DraIII CAC_nnn'GTG
Cuts at: 519 519
Size: 4046
DrdI GACnn_nn'nnGTC
Cuts at: 1752 2610 1752
Size: 858 3188
EaeI y'GGCC_r
Cuts at: 110 215 1033 1631 1805 2196 2223 3568 110
Size: 105 818 598 174 391 27 1345 588
Fragments arranged by size:
1345 818 598 588 391 174 105 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 490 967 2069 2279 490
Size: 477 1102 210 2257
Fragments arranged by size:
2257 1102 477 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2254 2564 2710 3856 2254
Size: 310 146 1146 2444
Fragments arranged by size:
2444 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1058 1058
Size: 4046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 424 613 762 1871 2303 3050 3318 271
Size: 153 189 149 1109 432 747 268 999
Fragments arranged by size:
1109 999 747 432 268 189 153 149
EcoO109I rG'GnC_Cy
Cuts at: 228 397 398 664 759 1185 228
Size: 169 1 266 95 426 3089
Fragments arranged by size:
3089 426 266 169 95 1
EcoRI G'AATT_C
Cuts at: 1050 1050
Size: 4046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 499 2065 499
Size: 1566 2480
FspI TGC'GCA
Cuts at: 1827 1827
Size: 4046
HaeII r_GCGC'y
Cuts at: 204 1480 1728 2750 3729 204
Size: 1276 248 1022 979 521
Fragments arranged by size:
1276 1022 979 521 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1888 1918 1920 1950 1888
Size: 30 2 30 3984
Fragments arranged by size:
3984 30 30 2
HincII GTy'rAC
Cuts at: 837 1085 837
Size: 248 3798
HindIII A'AGCT_T
Cuts at: 691 691
Size: 4046
KasI G'GCGC_C
Cuts at: 1476 1724 1476
Size: 248 3798
KpnI G_GTAC'C
Cuts at: 1066 1066
Size: 4046
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2717 2901 2717
Size: 184 3862
MscI TGG'CCA
Cuts at: 217 1807 217
Size: 1590 2456
MslI CAynn'nnrTG
Cuts at: 520 2035 2162 520
Size: 1515 127 2404
Fragments arranged by size:
2404 1515 127
MspA1I CmG'CkG
Cuts at: 115 259 300 341 624 897 968 1157 1471
Size: 144 41 41 283 273 71 189 314
Cuts at: 1471 1831 2844 3089 115
Size: 360 1013 245 1072
Fragments arranged by size:
1072 1013 360 314 283 273 245 189
144 71 41 41
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 4046
NarI GG'CG_CC
Cuts at: 1477 1725 1477
Size: 248 3798
NcoI C'CATG_G
Cuts at: 107 107
Size: 4046
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 4046
NspI r_CATG'y
Cuts at: 1099 1599 2506 1099
Size: 500 907 2639
Fragments arranged by size:
2639 907 500
PciI A'CATG_T
Cuts at: 2502 2502
Size: 4046
PflMI CCAn_nnn'nTGG
Cuts at: 213 519 213
Size: 306 3740
PfoI T'CCnGG_A
Cuts at: 755 778 755
Size: 23 4023
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PpuMI rG'GwC_Cy
Cuts at: 228 759 228
Size: 531 3515
PspOMI G'GGCC_C
Cuts at: 397 397
Size: 4046
PstI C_TGCA'G
Cuts at: 548 632 1093 1778 548
Size: 84 461 685 2816
Fragments arranged by size:
2816 685 461 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 259 300 341 624 1471 1831 259
Size: 41 41 283 847 360 2474
Fragments arranged by size:
2474 847 360 283 41 41
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 1060 1060
Size: 4046
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 548 1093 548
Size: 545 3501
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SexAI A'CCwGG_T
Cuts at: 206 206
Size: 4046
SfcI C'TryA_G
Cuts at: 33 544 628 1089 1774 2767 2958 33
Size: 511 84 461 685 993 191 1121
Fragments arranged by size:
1121 993 685 511 461 191 84
SfoI GGC'GCC
Cuts at: 1478 1726 1478
Size: 248 3798
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 1201 2116 2608 2870 1201
Size: 915 492 262 2377
Fragments arranged by size:
2377 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
StyI C'CwwG_G
Cuts at: 107 987 1180 1534 107
Size: 880 193 354 2619
Fragments arranged by size:
2619 880 354 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1629 1762 2221 2430 1629
Size: 133 459 209 3245
Fragments arranged by size:
3245 459 209 133
TatI w'GTAC_w
Cuts at: 3561 3561
Size: 4046
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4014
Tth111I GACn'n_nGTC
Cuts at: 1843 1843
Size: 4046
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 228 588 1541 228
Size: 360 953 2733
Fragments arranged by size:
2733 953 360
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I
BsaXI BsiWI BsmI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI