Restriction map for
pF1KB7232
(Circular) (Six-base) MAPSORT of: pF1KB7232.seq Check: 1905 from: 1 to: 4301
pF1KB7232 4301 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 704 704
Size: 4301
AccI GT'mk_AC
Cuts at: 1339 1339
Size: 4301
Acc65I G'GTAC_C
Cuts at: 1317 1317
Size: 4301
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 1005 2126 2558 3305 3573 541
Size: 464 1121 432 747 268 1269
Fragments arranged by size:
1269 1121 747 464 432 268
AflII C'TTAA_G
Cuts at: 982 982
Size: 4301
AflIII A'CryG_T
Cuts at: 834 1653 2757 3734 834
Size: 819 1104 977 1401
Fragments arranged by size:
1401 1104 977 819
AgeI A'CCGG_T
Cuts at: 398 2689 398
Size: 2291 2010
AhdI GACnn_n'nnGTC
Cuts at: 125 125
Size: 4301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 871 871
Size: 4301
AlwNI CAG_nnn'CTG
Cuts at: 1606 3173 1606
Size: 1567 2734
ApaLI G'TGCA_C
Cuts at: 3071 3071
Size: 4301
ApoI r'AATT_y
Cuts at: 661 1305 3654 3910 661
Size: 644 2349 256 1052
Fragments arranged by size:
2349 1052 644 256
AseI AT'TA_AT
Cuts at: 20 620 20
Size: 600 3701
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 1059 1321 1059
Size: 262 4039
BamHI G'GATC_C
Cuts at: 1326 1326
Size: 4301
BanI G'GyrC_C
Cuts at: 330 1317 1731 1979 2014 330
Size: 987 414 248 35 2617
Fragments arranged by size:
2617 987 414 248 35
BanII G_rGCy'C
Cuts at: 382 1315 2345 382
Size: 933 1030 2338
Fragments arranged by size:
2338 1030 933
BbeI G_GCGC'C
Cuts at: 1735 1983 1735
Size: 248 4053
BbsI GAAGACnn'nnnn_
Cuts at: 121 121
Size: 4301
BbvCI CC'TCA_GC
Cuts at: 546 546
Size: 4301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 186 220 186
Size: 34 4267
BciVI GTATCCnnnnn_n'
Cuts at: 2199 2960 4301 2199
Size: 761 1341 2199
Fragments arranged by size:
2199 1341 761
BclI T'GATC_A
Cuts at: 1110 1821 1110
Size: 711 3590
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4301
BglII A'GATC_T
Cuts at: 1816 3853 1816
Size: 2037 2264
BlpI GC'TnA_GC
Cuts at: 280 1413 280
Size: 1133 3168
Bme1580I G_kGCm'C
Cuts at: 335 1926 2019 3075 335
Size: 1591 93 1056 1561
Fragments arranged by size:
1591 1561 1056 93
BmgBI CAC'GTC
Cuts at: 1029 1029
Size: 4301
BmrI ACTGGGnnnn_n'
Cuts at: 1513 1594 1929 4073 1513
Size: 81 335 2144 1741
Fragments arranged by size:
2144 1741 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4301
Bpu10I CC'TnA_GC
Cuts at: 256 546 256
Size: 290 4011
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1477 2356 2848 3146 1477
Size: 879 492 298 2632
Fragments arranged by size:
2632 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 297 4005 297
Size: 3708 593
BsaAI yAC'GTr
Cuts at: 1014 1654 1014
Size: 640 3661
BsaBI GATnn'nnATC
Cuts at: 6 1815 3852 6
Size: 1809 2037 455
Fragments arranged by size:
2037 1809 455
BsaHI Gr'CG_yC
Cuts at: 214 323 1732 1980 2682 4178 214
Size: 109 1409 248 702 1496 337
Fragments arranged by size:
1496 1409 702 337 248 109
BsaWI w'CCGG_w
Cuts at: 183 398 1490 2011 2689 2963 3110 3521 183
Size: 215 1092 521 678 274 147 411 963
Fragments arranged by size:
1092 963 678 521 411 274 215 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 823 547
Size: 276 4025
BseYI C'CCAG_C
Cuts at: 189 236 313 1726 2484 3061 3557 189
Size: 47 77 1413 758 577 496 933
Fragments arranged by size:
1413 933 758 577 496 77 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 743 743
Size: 4301
BsiEI CG_ry'CG
Cuts at: 104 1843 1889 3097 104
Size: 1739 46 1208 1308
Fragments arranged by size:
1739 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1315 2093 2283 3075 1315
Size: 778 190 792 2541
Fragments arranged by size:
2541 792 778 190
BsmI GAATG_Cn'
Cuts at: 711 1096 711
Size: 385 3916
Bsp1286I G_dGCh'C
Cuts at: 335 382 1315 1926 2019 2093 2283 2345 3075
Size: 47 933 611 93 74 190 62 730
Cuts at: 3075 335
Size: 1561
Fragments arranged by size:
1561 933 730 611 190 93 74 62
47
BspEI T'CCGG_A
Cuts at: 183 3521 183
Size: 3338 963
BspHI T'CATG_A
Cuts at: 3477 3477
Size: 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 651 704 839 1351 1565 1867 2248 651
Size: 53 135 512 214 302 381 2704
Fragments arranged by size:
2704 512 381 302 214 135 53
BsrBI CCG'CTC
Cuts at: 50 2593 2647 4131 4296 50
Size: 2543 54 1484 165 55
Fragments arranged by size:
2543 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 892 2213 892
Size: 1321 2980
BsrFI r'CCGG_y
Cuts at: 398 2299 2480 2689 398
Size: 1901 181 209 2010
Fragments arranged by size:
2010 1901 209 181
BssSI C'ACGA_G
Cuts at: 2572 2930 2572
Size: 358 3943
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 1678
Size: 4301
BstBI TT'CG_AA
Cuts at: 2662 2662
Size: 4301
BstXI CCAn_nnnn'nTGG
Cuts at: 681 681
Size: 4301
BstYI r'GATC_y
Cuts at: 1 1326 1793 1816 2151 2397 3409 3495 3507
Size: 1325 467 23 335 246 1012 86 12
Cuts at: 3507 3853 1
Size: 346 449
Fragments arranged by size:
1325 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1178 1178
Size: 4301
BtgI C'CryG_G
Cuts at: 107 326 527 777 1083 107
Size: 219 201 250 306 3325
Fragments arranged by size:
3325 306 250 219 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2251 2432 2595 2251
Size: 181 163 3957
Fragments arranged by size:
3957 181 163
BtsI GCAGTG_nn'
Cuts at: 1544 1544
Size: 4301
DraI TTT'AAA
Cuts at: 1300 1300
Size: 4301
DrdI GACnn_nn'nnGTC
Cuts at: 2007 2865 2007
Size: 858 3443
EaeI y'GGCC_r
Cuts at: 113 909 1886 2060 2451 2478 3823 113
Size: 796 977 174 391 27 1345 591
Fragments arranged by size:
1345 977 796 591 391 174 27
EagI C'GGCC_G
Cuts at: 1886 1886
Size: 4301
EarI CTCTTCn'nnn_
Cuts at: 2324 2534 2324
Size: 210 4091
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2509 2819 2965 4111 2509
Size: 310 146 1146 2699
Fragments arranged by size:
2699 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1313 1313
Size: 4301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 1005 2126 2558 3305 3573 541
Size: 464 1121 432 747 268 1269
Fragments arranged by size:
1269 1121 747 464 432 268
EcoO109I rG'GnC_Cy
Cuts at: 119 153 252 1440 119
Size: 34 99 1188 2980
Fragments arranged by size:
2980 1188 99 34
EcoRI G'AATT_C
Cuts at: 1305 1305
Size: 4301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 869 2320 869
Size: 1451 2850
FspI TGC'GCA
Cuts at: 436 2082 436
Size: 1646 2655
HaeII r_GCGC'y
Cuts at: 1735 1983 3005 3984 1735
Size: 248 1022 979 2052
Fragments arranged by size:
2052 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 111 143 1038 1070 2143 2173 2175 2205 111
Size: 32 895 32 1073 30 2 30 2207
Fragments arranged by size:
2207 1073 895 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1155 1164 1340 1155
Size: 9 176 4116
Fragments arranged by size:
4116 176 9
HpaI GTT'AAC
Cuts at: 1164 1164
Size: 4301
KasI G'GCGC_C
Cuts at: 1731 1979 1731
Size: 248 4053
KpnI G_GTAC'C
Cuts at: 1321 1321
Size: 4301
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 383 2972 3156 383
Size: 2589 184 1528
Fragments arranged by size:
2589 1528 184
MscI TGG'CCA
Cuts at: 911 2062 911
Size: 1151 3150
MslI CAynn'nnrTG
Cuts at: 863 1273 2290 2417 863
Size: 410 1017 127 2747
Fragments arranged by size:
2747 1017 410 127
MspA1I CmG'CkG
Cuts at: 125 742 875 972 992 1412 1726 2086 3099
Size: 617 133 97 20 420 314 360 1013
Cuts at: 3099 3344 125
Size: 245 1082
Fragments arranged by size:
1082 1013 617 420 360 314 245 133
97 20
NaeI GCC'GGC
Cuts at: 2482 2482
Size: 4301
NarI GG'CG_CC
Cuts at: 1732 1980 1732
Size: 248 4053
NcoI C'CATG_G
Cuts at: 107 777 107
Size: 670 3631
NgoMIV G'CCGG_C
Cuts at: 2480 2480
Size: 4301
NspI r_CATG'y
Cuts at: 288 345 838 1187 1354 1854 2761 288
Size: 57 493 349 167 500 907 1828
Fragments arranged by size:
1828 907 500 493 349 167 57
PciI A'CATG_T
Cuts at: 834 2757 834
Size: 1923 2378
PflMI CCAn_nnn'nTGG
Cuts at: 402 402
Size: 4301
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 4269
PpuMI rG'GwC_Cy
Cuts at: 119 153 252 119
Size: 34 99 4168
Fragments arranged by size:
4168 99 34
PshAI GACnn'nnGTC
Cuts at: 1150 1150
Size: 4301
PstI C_TGCA'G
Cuts at: 1348 2033 1348
Size: 685 3616
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4301
PvuII CAG'CTG
Cuts at: 875 992 1726 2086 875
Size: 117 734 360 3090
Fragments arranged by size:
3090 734 360 117
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 1315 1315
Size: 4301
SalI G'TCGA_C
Cuts at: 1338 1338
Size: 4301
SanDI GG'GwC_CC
Cuts at: 119 119
Size: 4301
SapI GCTCTTCn'nnn_
Cuts at: 2324 2534 2324
Size: 210 4091
SbfI CC_TGCA'GG
Cuts at: 1348 1348
Size: 4301
ScaI AGT'ACT
Cuts at: 3818 3818
Size: 4301
SexAI A'CCwGG_T
Cuts at: 688 688
Size: 4301
SfcI C'TryA_G
Cuts at: 33 1344 2029 3022 3213 33
Size: 1311 685 993 191 1121
Fragments arranged by size:
1311 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1733 1981 1733
Size: 248 4053
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SmaI CCC'GGG
Cuts at: 1061 1323 1061
Size: 262 4039
SmlI C'TyrA_G
Cuts at: 982 1456 2371 2863 3125 982
Size: 474 915 492 262 2158
Fragments arranged by size:
2158 915 492 474 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 1187 1354 1854 1187
Size: 167 500 3634
Fragments arranged by size:
3634 500 167
SspI AAT'ATT
Cuts at: 624 624
Size: 4301
StyI C'CwwG_G
Cuts at: 107 777 1435 1789 107
Size: 670 658 354 2619
Fragments arranged by size:
2619 670 658 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1277 1884 2017 2476 2685 1277
Size: 607 133 459 209 2893
Fragments arranged by size:
2893 607 459 209 133
TatI w'GTAC_w
Cuts at: 3816 3816
Size: 4301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 773 805 3818 3850 773
Size: 32 3013 32 1224
Fragments arranged by size:
3013 1224 32 32
Tth111I GACn'n_nGTC
Cuts at: 250 2098 250
Size: 1848 2453
XbaI T'CTAG_A
Cuts at: 1332 1332
Size: 4301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1796 1796
Size: 4301
XmaI C'CCGG_G
Cuts at: 1059 1321 1059
Size: 262 4039
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AleI AloI ApaI AscI AvrII BaeI
BfrBI BmtI BpmI BsaXI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI