Restriction map for
pF1KB7230
(Circular) (Six-base) MAPSORT of: pF1KB7230.seq Check: 119 from: 1 to: 4166
pF1KB7230 4166 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1204 1204
Size: 4166
Acc65I G'GTAC_C
Cuts at: 1182 1182
Size: 4166
AclI AA'CG_TT
Cuts at: 4006 4006
Size: 4166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1991 2423 3170 3438 1105
Size: 886 432 747 268 1833
Fragments arranged by size:
1833 886 747 432 268
AflIII A'CryG_T
Cuts at: 1033 1518 2622 3599 1033
Size: 485 1104 977 1600
Fragments arranged by size:
1600 1104 977 485
AgeI A'CCGG_T
Cuts at: 2554 2554
Size: 4166
AlwNI CAG_nnn'CTG
Cuts at: 707 1471 3038 707
Size: 764 1567 1835
Fragments arranged by size:
1835 1567 764
ApaLI G'TGCA_C
Cuts at: 2936 2936
Size: 4166
ApoI r'AATT_y
Cuts at: 172 800 1106 1170 3519 3775 172
Size: 628 306 64 2349 256 563
Fragments arranged by size:
2349 628 563 306 256 64
AseI AT'TA_AT
Cuts at: 20 20
Size: 4166
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
AvaI C'yCGr_G
Cuts at: 1186 1186
Size: 4166
BamHI G'GATC_C
Cuts at: 262 960 1191 262
Size: 698 231 3237
Fragments arranged by size:
3237 698 231
BanI G'GyrC_C
Cuts at: 1182 1596 1844 1879 1182
Size: 414 248 35 3469
Fragments arranged by size:
3469 414 248 35
BanII G_rGCy'C
Cuts at: 1180 2210 1180
Size: 1030 3136
BbeI G_GCGC'C
Cuts at: 1600 1848 1600
Size: 248 3918
BbsI GAAGACnn'nnnn_
Cuts at: 636 636
Size: 4166
BciVI GTATCCnnnnn_n'
Cuts at: 822 2064 2825 4166 822
Size: 1242 761 1341 822
Fragments arranged by size:
1341 1242 822 761
BclI T'GATC_A
Cuts at: 410 1686 410
Size: 1276 2890
BglII A'GATC_T
Cuts at: 199 220 1681 3718 199
Size: 21 1461 2037 647
Fragments arranged by size:
2037 1461 647 21
BlpI GC'TnA_GC
Cuts at: 484 1278 484
Size: 794 3372
Bme1580I G_kGCm'C
Cuts at: 1791 1884 2940 1791
Size: 93 1056 3017
Fragments arranged by size:
3017 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 706 1378 1459 1794 3938 706
Size: 672 81 335 2144 934
Fragments arranged by size:
2144 934 672 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 641
Size: 4166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 2221 2713 3011 1342
Size: 879 492 298 2497
Fragments arranged by size:
2497 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3870 3870
Size: 4166
BsaAI yAC'GTr
Cuts at: 1519 1519
Size: 4166
BsaBI GATnn'nnATC
Cuts at: 6 1680 3717 6
Size: 1674 2037 455
Fragments arranged by size:
2037 1674 455
BsaHI Gr'CG_yC
Cuts at: 1597 1845 2547 4043 1597
Size: 248 702 1496 1720
Fragments arranged by size:
1720 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1355 1876 2554 2828 2975 3386 1355
Size: 521 678 274 147 411 2135
Fragments arranged by size:
2135 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1591 2349 2926 3422 1591
Size: 758 577 496 2335
Fragments arranged by size:
2335 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1708 1754 2962 104
Size: 1604 46 1208 1308
Fragments arranged by size:
1604 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1083 1180 1958 2148 2940 1083
Size: 97 778 190 792 2309
Fragments arranged by size:
2309 792 778 190 97
Bsp1286I G_dGCh'C
Cuts at: 1083 1180 1791 1884 1958 2148 2210 2940 1083
Size: 97 611 93 74 190 62 730 2309
Fragments arranged by size:
2309 730 611 190 97 93 74 62
BspEI T'CCGG_A
Cuts at: 3386 3386
Size: 4166
BspHI T'CATG_A
Cuts at: 3342 3342
Size: 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 745 1216 1430 1732 2113 745
Size: 471 214 302 381 2798
Fragments arranged by size:
2798 471 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2458 2512 3996 4161 50
Size: 2408 54 1484 165 55
Fragments arranged by size:
2408 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2078 2078
Size: 4166
BsrFI r'CCGG_y
Cuts at: 2164 2345 2554 2164
Size: 181 209 3776
Fragments arranged by size:
3776 209 181
BssSI C'ACGA_G
Cuts at: 2437 2795 2437
Size: 358 3808
BstAPI GCAn_nnn'nTGC
Cuts at: 1543 1543
Size: 4166
BstBI TT'CG_AA
Cuts at: 2527 2527
Size: 4166
BstYI r'GATC_y
Cuts at: 1 199 220 262 960 1191 1658 1681 2016
Size: 198 21 42 698 231 467 23 335
Cuts at: 2016 2262 3274 3360 3372 3718 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 698 467 449 346 335 246 231
198 86 42 23 21 12
BtgI C'CryG_G
Cuts at: 107 359 107
Size: 252 3914
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2116 2297 2460 2116
Size: 181 163 3822
Fragments arranged by size:
3822 181 163
BtsI GCAGTG_nn'
Cuts at: 1409 1409
Size: 4166
DraI TTT'AAA
Cuts at: 1165 1165
Size: 4166
DrdI GACnn_nn'nnGTC
Cuts at: 1872 2730 1872
Size: 858 3308
EaeI y'GGCC_r
Cuts at: 362 518 1751 1925 2316 2343 3688 362
Size: 156 1233 174 391 27 1345 840
Fragments arranged by size:
1345 1233 840 391 174 156 27
EagI C'GGCC_G
Cuts at: 1751 1751
Size: 4166
EarI CTCTTCn'nnn_
Cuts at: 2189 2399 2189
Size: 210 3956
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2374 2684 2830 3976 2374
Size: 310 146 1146 2564
Fragments arranged by size:
2564 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1178 1178
Size: 4166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 1105 1991 2423 3170 3438 641
Size: 464 886 432 747 268 1369
Fragments arranged by size:
1369 886 747 464 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1305 1305
Size: 4166
EcoRI G'AATT_C
Cuts at: 1170 1170
Size: 4166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 4166
FspI TGC'GCA
Cuts at: 1947 1947
Size: 4166
HaeII r_GCGC'y
Cuts at: 1600 1848 2870 3849 1600
Size: 248 1022 979 1917
Fragments arranged by size:
1917 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 377 409 2008 2038 2040 2070 377
Size: 32 1599 30 2 30 2473
Fragments arranged by size:
2473 1599 32 30 30 2
HincII GTy'rAC
Cuts at: 1205 1205
Size: 4166
KasI G'GCGC_C
Cuts at: 1596 1844 1596
Size: 248 3918
KpnI G_GTAC'C
Cuts at: 1186 1186
Size: 4166
MfeI C'AATT_G
Cuts at: 177 177
Size: 4166
MluI A'CGCG_T
Cuts at: 3599 3599
Size: 4166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2837 3021 2837
Size: 184 3982
MscI TGG'CCA
Cuts at: 364 520 1927 364
Size: 156 1407 2603
Fragments arranged by size:
2603 1407 156
MslI CAynn'nnrTG
Cuts at: 394 827 2155 2282 394
Size: 433 1328 127 2278
Fragments arranged by size:
2278 1328 433 127
MspA1I CmG'CkG
Cuts at: 1277 1591 1951 2964 3209 1277
Size: 314 360 1013 245 2234
Fragments arranged by size:
2234 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2347 2347
Size: 4166
NarI GG'CG_CC
Cuts at: 1597 1845 1597
Size: 248 3918
NcoI C'CATG_G
Cuts at: 107 359 107
Size: 252 3914
NgoMIV G'CCGG_C
Cuts at: 2345 2345
Size: 4166
NspI r_CATG'y
Cuts at: 826 1037 1219 1719 2626 826
Size: 211 182 500 907 2366
Fragments arranged by size:
2366 907 500 211 182
PciI A'CATG_T
Cuts at: 1033 2622 1033
Size: 1589 2577
PmeI GTTT'AAAC
Cuts at: 1165 1165
Size: 4166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 4134
PstI C_TGCA'G
Cuts at: 1213 1898 1213
Size: 685 3481
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4166
PvuII CAG'CTG
Cuts at: 1591 1951 1591
Size: 360 3806
RsrII CG'GwC_CG
Cuts at: 2361 2361
Size: 4166
SacI G_AGCT'C
Cuts at: 1180 1180
Size: 4166
SalI G'TCGA_C
Cuts at: 1203 1203
Size: 4166
SapI GCTCTTCn'nnn_
Cuts at: 2189 2399 2189
Size: 210 3956
SbfI CC_TGCA'GG
Cuts at: 1213 1213
Size: 4166
ScaI AGT'ACT
Cuts at: 3683 3683
Size: 4166
SfcI C'TryA_G
Cuts at: 33 986 1209 1894 2887 3078 33
Size: 953 223 685 993 191 1121
Fragments arranged by size:
1121 993 953 685 223 191
SfoI GGC'GCC
Cuts at: 1598 1846 1598
Size: 248 3918
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
SmaI CCC'GGG
Cuts at: 1188 1188
Size: 4166
SmlI C'TyrA_G
Cuts at: 1321 2236 2728 2990 1321
Size: 915 492 262 2497
Fragments arranged by size:
2497 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4166
SphI G_CATG'C
Cuts at: 1219 1719 1219
Size: 500 3666
SspI AAT'ATT
Cuts at: 281 281
Size: 4166
StuI AGG'CCT
Cuts at: 655 655
Size: 4166
StyI C'CwwG_G
Cuts at: 107 359 1300 1654 107
Size: 252 941 354 2619
Fragments arranged by size:
2619 941 354 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 217 603 1749 1882 2341 2550 217
Size: 386 1146 133 459 209 1833
Fragments arranged by size:
1833 1146 459 386 209 133
TatI w'GTAC_w
Cuts at: 1088 3681 1088
Size: 2593 1573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1023 1055 3683 3715 1023
Size: 32 2628 32 1474
Fragments arranged by size:
2628 1474 32 32
Tth111I GACn'n_nGTC
Cuts at: 1963 1963
Size: 4166
XbaI T'CTAG_A
Cuts at: 1197 1197
Size: 4166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 747 1661 747
Size: 914 3252
XmaI C'CCGG_G
Cuts at: 1186 1186
Size: 4166
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI ZraI