Restriction map for
pF1KB7229
(Circular) (Six-base) MAPSORT of: pF1KB7229.seq Check: 2668 from: 1 to: 4232
pF1KB7229 4232 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 805 805
Size: 4232
AccI GT'mk_AC
Cuts at: 677 1270 677
Size: 593 3639
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 417 826 843 2057 2489 3236 3504 417
Size: 409 17 1214 432 747 268 1145
Fragments arranged by size:
1214 1145 747 432 409 268 17
AflIII A'CryG_T
Cuts at: 1584 2688 3665 1584
Size: 1104 977 2151
Fragments arranged by size:
2151 1104 977
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AleI CACnn'nnGTG
Cuts at: 248 652 1111 248
Size: 404 459 3369
Fragments arranged by size:
3369 459 404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 277 316 592 277
Size: 39 276 3917
Fragments arranged by size:
3917 276 39
AlwNI CAG_nnn'CTG
Cuts at: 1537 3104 1537
Size: 1567 2665
ApaI G_GGCC'C
Cuts at: 178 178
Size: 4232
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 1236 3585 3841 1236
Size: 2349 256 1627
Fragments arranged by size:
2349 1627 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 721 1252 721
Size: 531 3701
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 136 1248 1662 1910 1945 136
Size: 1112 414 248 35 2423
Fragments arranged by size:
2423 1112 414 248 35
BanII G_rGCy'C
Cuts at: 178 1246 2276 178
Size: 1068 1030 2134
Fragments arranged by size:
2134 1068 1030
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BbsI GAAGACnn'nnnn_
Cuts at: 747 747
Size: 4232
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 821 1344 821
Size: 523 3709
Bme1580I G_kGCm'C
Cuts at: 178 1857 1950 3006 178
Size: 1679 93 1056 1404
Fragments arranged by size:
1679 1404 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 880 1444 1525 1860 4004 880
Size: 564 81 335 2144 1108
Fragments arranged by size:
2144 1108 564 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 980 2599 980
Size: 1619 2613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 721 759 532
Size: 189 38 4005
Fragments arranged by size:
4005 189 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 2287 2779 3077 1408
Size: 879 492 298 2563
Fragments arranged by size:
2563 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 204 688 3936 204
Size: 484 3248 500
Fragments arranged by size:
3248 500 484
BsaAI yAC'GTr
Cuts at: 576 1585 576
Size: 1009 3223
BsaBI GATnn'nnATC
Cuts at: 6 364 790 1746 3783 6
Size: 358 426 956 2037 455
Fragments arranged by size:
2037 956 455 426 358
BsaHI Gr'CG_yC
Cuts at: 1663 1911 2613 4109 1663
Size: 248 702 1496 1786
Fragments arranged by size:
1786 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1421 1942 2620 2894 3041 3452 1421
Size: 521 678 274 147 411 2201
Fragments arranged by size:
2201 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 469 502 469
Size: 33 4199
BseYI C'CCAG_C
Cuts at: 1122 1657 2415 2992 3488 1122
Size: 535 758 577 496 1866
Fragments arranged by size:
1866 758 577 535 496
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3028 104
Size: 1670 46 1208 1308
Fragments arranged by size:
1670 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1246 2024 2214 3006 1246
Size: 778 190 792 2472
Fragments arranged by size:
2472 792 778 190
BsmI GAATG_Cn'
Cuts at: 636 703 636
Size: 67 4165
Bsp1286I G_dGCh'C
Cuts at: 178 1246 1857 1950 2024 2214 2276 3006 178
Size: 1068 611 93 74 190 62 730 1404
Fragments arranged by size:
1404 1068 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 971 3408 971
Size: 2437 1795
BspMI ACCTGCnnnn'nnnn_
Cuts at: 255 805 1282 1496 1798 2179 255
Size: 550 477 214 302 381 2308
Fragments arranged by size:
2308 550 477 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2524 2578 4062 4227 50
Size: 2474 54 1484 165 55
Fragments arranged by size:
2474 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 399 2144 399
Size: 1745 2487
BsrFI r'CCGG_y
Cuts at: 2230 2411 2620 2230
Size: 181 209 3842
Fragments arranged by size:
3842 209 181
BsrGI T'GTAC_A
Cuts at: 287 287
Size: 4232
BssSI C'ACGA_G
Cuts at: 244 2503 2861 244
Size: 2259 358 1615
Fragments arranged by size:
2259 1615 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1609 1609
Size: 4232
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstEII G'GTnAC_C
Cuts at: 810 1069 810
Size: 259 3973
BstXI CCAn_nnnn'nTGG
Cuts at: 114 250 114
Size: 136 4096
BstYI r'GATC_y
Cuts at: 1 1257 1724 1747 2082 2328 3340 3426 3438
Size: 1256 467 23 335 246 1012 86 12
Cuts at: 3438 3784 1
Size: 346 449
Fragments arranged by size:
1256 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 203 320 440 663 1037 107
Size: 96 117 120 223 374 3302
Fragments arranged by size:
3302 374 223 120 117 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2182 2363 2526 2182
Size: 181 163 3888
Fragments arranged by size:
3888 181 163
BtsI GCAGTG_nn'
Cuts at: 580 600 1093 1196 1475 580
Size: 20 493 103 279 3337
Fragments arranged by size:
3337 493 279 103 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 569 604 569
Size: 35 4197
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DraIII CAC_nnn'GTG
Cuts at: 606 1212 606
Size: 606 3626
DrdI GACnn_nn'nnGTC
Cuts at: 1938 2796 1938
Size: 858 3374
EaeI y'GGCC_r
Cuts at: 608 1817 1991 2382 2409 3754 608
Size: 1209 174 391 27 1345 1086
Fragments arranged by size:
1345 1209 1086 391 174 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 502 702 2255 2465 502
Size: 200 1553 210 2269
Fragments arranged by size:
2269 1553 210 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2440 2750 2896 4042 2440
Size: 310 146 1146 2630
Fragments arranged by size:
2630 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1244 1244
Size: 4232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 417 532 721 759 826 843 2057 2489 3236
Size: 115 189 38 67 17 1214 432 747
Cuts at: 3236 3504 417
Size: 268 1145
Fragments arranged by size:
1214 1145 747 432 268 189 115 67
38 17
EcoO109I rG'GnC_Cy
Cuts at: 512 828 1371 512
Size: 316 543 3373
Fragments arranged by size:
3373 543 316
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 4232
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 1666 1914 2936 3915 1666
Size: 248 1022 979 1983
Fragments arranged by size:
1983 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 456 488 496 528 2074 2104 2106 2136 456
Size: 32 8 32 1546 30 2 30 2552
Fragments arranged by size:
2552 1546 32 32 30 30 8 2
HincII GTy'rAC
Cuts at: 678 1271 678
Size: 593 3639
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 216 896 2903 3087 216
Size: 680 2007 184 1361
Fragments arranged by size:
2007 1361 680 184
MscI TGG'CCA
Cuts at: 610 1993 610
Size: 1383 2849
MslI CAynn'nnrTG
Cuts at: 112 248 652 1111 2221 2348 112
Size: 136 404 459 1110 127 1996
Fragments arranged by size:
1996 1110 459 404 136 127
MspA1I CmG'CkG
Cuts at: 878 1343 1657 2017 3030 3275 878
Size: 465 314 360 1013 245 1835
Fragments arranged by size:
1835 1013 465 360 314 245
NaeI GCC'GGC
Cuts at: 2413 2413
Size: 4232
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NcoI C'CATG_G
Cuts at: 107 203 663 107
Size: 96 460 3676
Fragments arranged by size:
3676 460 96
NgoMIV G'CCGG_C
Cuts at: 2411 2411
Size: 4232
NspI r_CATG'y
Cuts at: 264 1015 1285 1785 2692 264
Size: 751 270 500 907 1804
Fragments arranged by size:
1804 907 751 500 270
PciI A'CATG_T
Cuts at: 2688 2688
Size: 4232
PflMI CCAn_nnn'nTGG
Cuts at: 606 878 606
Size: 272 3960
PfoI T'CCnGG_A
Cuts at: 952 1055 952
Size: 103 4129
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PmlI CAC'GTG
Cuts at: 576 576
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PpuMI rG'GwC_Cy
Cuts at: 512 828 512
Size: 316 3916
PshAI GACnn'nnGTC
Cuts at: 908 908
Size: 4232
PspOMI G'GGCC_C
Cuts at: 174 174
Size: 4232
PstI C_TGCA'G
Cuts at: 1279 1964 1279
Size: 685 3547
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 878 1657 2017 878
Size: 779 360 3093
Fragments arranged by size:
3093 779 360
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 1246 1246
Size: 4232
SalI G'TCGA_C
Cuts at: 676 1269 676
Size: 593 3639
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SexAI A'CCwGG_T
Cuts at: 421 421
Size: 4232
SfcI C'TryA_G
Cuts at: 33 1275 1960 2953 3144 33
Size: 1242 685 993 191 1121
Fragments arranged by size:
1242 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 723 1254 723
Size: 531 3701
SmlI C'TyrA_G
Cuts at: 1387 2302 2794 3056 1387
Size: 915 492 262 2563
Fragments arranged by size:
2563 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 264 1285 1785 264
Size: 1021 500 2711
Fragments arranged by size:
2711 1021 500
StuI AGG'CCT
Cuts at: 184 850 1133 184
Size: 666 283 3283
Fragments arranged by size:
3283 666 283
StyI C'CwwG_G
Cuts at: 107 170 203 663 1366 1720 107
Size: 63 33 460 703 354 2619
Fragments arranged by size:
2619 703 460 354 63 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1815 1948 2407 2616 1815
Size: 133 459 209 3431
Fragments arranged by size:
3431 459 209 133
TatI w'GTAC_w
Cuts at: 287 3747 287
Size: 3460 772
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1037 1069 3749 3781 1037
Size: 32 2680 32 1488
Fragments arranged by size:
2680 1488 32 32
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 525 1727 525
Size: 1202 3030
XmaI C'CCGG_G
Cuts at: 721 1252 721
Size: 531 3701
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmBI BssHII BstZ17I Bsu36I ClaI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI