Restriction map for
pF1KB7225
(Circular) (Six-base) MAPSORT of: pF1KB7225.seq Check: 1536 from: 1 to: 3899
pF1KB7225 3899 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 937 937
Size: 3899
Acc65I G'GTAC_C
Cuts at: 915 915
Size: 3899
AclI AA'CG_TT
Cuts at: 3739 3739
Size: 3899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 299 674 1724 2156 2903 3171 142
Size: 157 375 1050 432 747 268 870
Fragments arranged by size:
1050 870 747 432 375 268 157
AflIII A'CryG_T
Cuts at: 1251 2355 3332 1251
Size: 1104 977 1818
Fragments arranged by size:
1818 1104 977
AgeI A'CCGG_T
Cuts at: 2287 2287
Size: 3899
AlwNI CAG_nnn'CTG
Cuts at: 1204 2771 1204
Size: 1567 2332
ApaI G_GGCC'C
Cuts at: 582 582
Size: 3899
ApaLI G'TGCA_C
Cuts at: 2669 2669
Size: 3899
ApoI r'AATT_y
Cuts at: 850 903 3252 3508 850
Size: 53 2349 256 1241
Fragments arranged by size:
2349 1241 256 53
AseI AT'TA_AT
Cuts at: 20 221 20
Size: 201 3698
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3899
AvaI C'yCGr_G
Cuts at: 551 919 551
Size: 368 3531
BamHI G'GATC_C
Cuts at: 924 924
Size: 3899
BanI G'GyrC_C
Cuts at: 915 1329 1577 1612 915
Size: 414 248 35 3202
Fragments arranged by size:
3202 414 248 35
BanII G_rGCy'C
Cuts at: 582 913 1943 582
Size: 331 1030 2538
Fragments arranged by size:
2538 1030 331
BbeI G_GCGC'C
Cuts at: 1333 1581 1333
Size: 248 3651
BciVI GTATCCnnnnn_n'
Cuts at: 1797 2558 3899 1797
Size: 761 1341 1797
Fragments arranged by size:
1797 1341 761
BclI T'GATC_A
Cuts at: 1419 1419
Size: 3899
BglII A'GATC_T
Cuts at: 1414 3451 1414
Size: 2037 1862
BlpI GC'TnA_GC
Cuts at: 1011 1011
Size: 3899
Bme1580I G_kGCm'C
Cuts at: 582 1524 1617 2673 582
Size: 942 93 1056 1808
Fragments arranged by size:
1808 1056 942 93
BmgBI CAC'GTC
Cuts at: 742 742
Size: 3899
BmrI ACTGGGnnnn_n'
Cuts at: 221 1111 1192 1527 3671 221
Size: 890 81 335 2144 449
Fragments arranged by size:
2144 890 449 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 3899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1954 2446 2744 1075
Size: 879 492 298 2230
Fragments arranged by size:
2230 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3603 3603
Size: 3899
BsaAI yAC'GTr
Cuts at: 1252 1252
Size: 3899
BsaBI GATnn'nnATC
Cuts at: 6 1413 3450 6
Size: 1407 2037 455
Fragments arranged by size:
2037 1407 455
BsaHI Gr'CG_yC
Cuts at: 1330 1578 2280 3776 1330
Size: 248 702 1496 1453
Fragments arranged by size:
1496 1453 702 248
BsaWI w'CCGG_w
Cuts at: 587 1088 1609 2287 2561 2708 3119 587
Size: 501 521 678 274 147 411 1367
Fragments arranged by size:
1367 678 521 501 411 274 147
BseYI C'CCAG_C
Cuts at: 1324 2082 2659 3155 1324
Size: 758 577 496 2068
Fragments arranged by size:
2068 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1441 1487 2695 104
Size: 1337 46 1208 1308
Fragments arranged by size:
1337 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 913 1691 1881 2673 913
Size: 778 190 792 2139
Fragments arranged by size:
2139 792 778 190
BsmI GAATG_Cn'
Cuts at: 709 709
Size: 3899
Bsp1286I G_dGCh'C
Cuts at: 582 913 1524 1617 1691 1881 1943 2673 582
Size: 331 611 93 74 190 62 730 1808
Fragments arranged by size:
1808 730 611 331 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3119 3119
Size: 3899
BspHI T'CATG_A
Cuts at: 3075 3075
Size: 3899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 949 1163 1465 1846 949
Size: 214 302 381 3002
Fragments arranged by size:
3002 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2191 2245 3729 3894 50
Size: 2141 54 1484 165 55
Fragments arranged by size:
2141 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 212 1811 212
Size: 1599 2300
BsrFI r'CCGG_y
Cuts at: 1897 2078 2287 1897
Size: 181 209 3509
Fragments arranged by size:
3509 209 181
BsrGI T'GTAC_A
Cuts at: 775 775
Size: 3899
BssSI C'ACGA_G
Cuts at: 2170 2528 2170
Size: 358 3541
BstAPI GCAn_nnn'nTGC
Cuts at: 1276 1276
Size: 3899
BstBI TT'CG_AA
Cuts at: 2260 2260
Size: 3899
BstYI r'GATC_y
Cuts at: 1 793 924 1391 1414 1749 1995 3007 3093
Size: 792 131 467 23 335 246 1012 86
Cuts at: 3093 3105 3451 1
Size: 12 346 449
Fragments arranged by size:
1012 792 467 449 346 335 246 131
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3899
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1849 2030 2193 1849
Size: 181 163 3555
Fragments arranged by size:
3555 181 163
BtsI GCAGTG_nn'
Cuts at: 1142 1142
Size: 3899
DraI TTT'AAA
Cuts at: 898 898
Size: 3899
DrdI GACnn_nn'nnGTC
Cuts at: 1605 2463 1605
Size: 858 3041
EaeI y'GGCC_r
Cuts at: 1484 1658 2049 2076 3421 1484
Size: 174 391 27 1345 1962
Fragments arranged by size:
1962 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1484 1484
Size: 3899
EarI CTCTTCn'nnn_
Cuts at: 139 1922 2132 139
Size: 1783 210 1906
Fragments arranged by size:
1906 1783 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2107 2417 2563 3709 2107
Size: 310 146 1146 2297
Fragments arranged by size:
2297 1146 310 146
EcoICRI GAG'CTC
Cuts at: 911 911
Size: 3899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 299 674 1724 2156 2903 3171 142
Size: 157 375 1050 432 747 268 870
Fragments arranged by size:
1050 870 747 432 375 268 157
EcoO109I rG'GnC_Cy
Cuts at: 1038 1038
Size: 3899
EcoRI G'AATT_C
Cuts at: 903 903
Size: 3899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1918 1918
Size: 3899
FspI TGC'GCA
Cuts at: 1680 1680
Size: 3899
HaeII r_GCGC'y
Cuts at: 1333 1581 2603 3582 1333
Size: 248 1022 979 1650
Fragments arranged by size:
1650 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 551 583 1741 1771 1773 1803 551
Size: 32 1158 30 2 30 2647
Fragments arranged by size:
2647 1158 32 30 30 2
HincII GTy'rAC
Cuts at: 524 938 524
Size: 414 3485
KasI G'GCGC_C
Cuts at: 1329 1577 1329
Size: 248 3651
KpnI G_GTAC'C
Cuts at: 919 919
Size: 3899
MluI A'CGCG_T
Cuts at: 3332 3332
Size: 3899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2570 2754 2570
Size: 184 3715
MscI TGG'CCA
Cuts at: 1660 1660
Size: 3899
MslI CAynn'nnrTG
Cuts at: 1888 2015 1888
Size: 127 3772
MspA1I CmG'CkG
Cuts at: 719 1010 1324 1684 2697 2942 719
Size: 291 314 360 1013 245 1676
Fragments arranged by size:
1676 1013 360 314 291 245
NaeI GCC'GGC
Cuts at: 2080 2080
Size: 3899
NarI GG'CG_CC
Cuts at: 1330 1578 1330
Size: 248 3651
NcoI C'CATG_G
Cuts at: 107 107
Size: 3899
NdeI CA'TA_TG
Cuts at: 399 399
Size: 3899
NgoMIV G'CCGG_C
Cuts at: 2078 2078
Size: 3899
NspI r_CATG'y
Cuts at: 952 1452 2359 952
Size: 500 907 2492
Fragments arranged by size:
2492 907 500
PciI A'CATG_T
Cuts at: 2355 2355
Size: 3899
PmeI GTTT'AAAC
Cuts at: 898 898
Size: 3899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3038 3070 3038
Size: 32 3867
PspOMI G'GGCC_C
Cuts at: 578 578
Size: 3899
PspXI vC'TCGA_Gb
Cuts at: 551 551
Size: 3899
PstI C_TGCA'G
Cuts at: 946 1631 946
Size: 685 3214
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3899
PvuII CAG'CTG
Cuts at: 719 1324 1684 719
Size: 605 360 2934
Fragments arranged by size:
2934 605 360
RsrII CG'GwC_CG
Cuts at: 2094 2094
Size: 3899
SacI G_AGCT'C
Cuts at: 913 913
Size: 3899
SalI G'TCGA_C
Cuts at: 936 936
Size: 3899
SapI GCTCTTCn'nnn_
Cuts at: 1922 2132 1922
Size: 210 3689
SbfI CC_TGCA'GG
Cuts at: 946 946
Size: 3899
ScaI AGT'ACT
Cuts at: 376 3416 376
Size: 3040 859
SfcI C'TryA_G
Cuts at: 33 942 1627 2620 2811 33
Size: 909 685 993 191 1121
Fragments arranged by size:
1121 993 909 685 191
SfoI GGC'GCC
Cuts at: 1331 1579 1331
Size: 248 3651
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3899
SmaI CCC'GGG
Cuts at: 921 921
Size: 3899
SmlI C'TyrA_G
Cuts at: 551 1054 1969 2461 2723 551
Size: 503 915 492 262 1727
Fragments arranged by size:
1727 915 503 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3899
SphI G_CATG'C
Cuts at: 952 1452 952
Size: 500 3399
StyI C'CwwG_G
Cuts at: 107 806 1033 1387 107
Size: 699 227 354 2619
Fragments arranged by size:
2619 699 354 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1482 1615 2074 2283 1482
Size: 133 459 209 3098
Fragments arranged by size:
3098 459 209 133
TatI w'GTAC_w
Cuts at: 374 775 856 3414 374
Size: 401 81 2558 859
Fragments arranged by size:
2558 859 401 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 3867
Tth111I GACn'n_nGTC
Cuts at: 1696 1696
Size: 3899
XbaI T'CTAG_A
Cuts at: 930 930
Size: 3899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1394 1394
Size: 3899
XhoI C'TCGA_G
Cuts at: 551 551
Size: 3899
XmaI C'CCGG_G
Cuts at: 919 919
Size: 3899
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI
PpiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI