Restriction map for
pF1KB7224
(Circular) (Six-base) MAPSORT of: pF1KB7224.seq Check: 6263 from: 1 to: 4181
pF1KB7224 4181 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 145 282 145
Size: 137 4044
AccI GT'mk_AC
Cuts at: 1219 1219
Size: 4181
Acc65I G'GTAC_C
Cuts at: 1197 1197
Size: 4181
AclI AA'CG_TT
Cuts at: 4021 4021
Size: 4181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 1001 2006 2438 3185 3453 461
Size: 540 1005 432 747 268 1189
Fragments arranged by size:
1189 1005 747 540 432 268
AflIII A'CryG_T
Cuts at: 1533 2637 3614 1533
Size: 1104 977 2100
Fragments arranged by size:
2100 1104 977
AgeI A'CCGG_T
Cuts at: 2569 2569
Size: 4181
AleI CACnn'nnGTG
Cuts at: 559 559
Size: 4181
AlwNI CAG_nnn'CTG
Cuts at: 1486 3053 1486
Size: 1567 2614
ApaI G_GGCC'C
Cuts at: 459 687 459
Size: 228 3953
ApaLI G'TGCA_C
Cuts at: 416 2951 416
Size: 2535 1646
ApoI r'AATT_y
Cuts at: 1185 3534 3790 1185
Size: 2349 256 1576
Fragments arranged by size:
2349 1576 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4181
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
AvaI C'yCGr_G
Cuts at: 674 1201 674
Size: 527 3654
BamHI G'GATC_C
Cuts at: 1206 1206
Size: 4181
BanI G'GyrC_C
Cuts at: 1197 1611 1859 1894 1197
Size: 414 248 35 3484
Fragments arranged by size:
3484 414 248 35
BanII G_rGCy'C
Cuts at: 459 545 687 1195 2225 459
Size: 86 142 508 1030 2415
Fragments arranged by size:
2415 1030 508 142 86
BbeI G_GCGC'C
Cuts at: 1615 1863 1615
Size: 248 3933
BbvCI CC'TCA_GC
Cuts at: 301 625 823 973 301
Size: 324 198 150 3509
Fragments arranged by size:
3509 324 198 150
BciVI GTATCCnnnnn_n'
Cuts at: 2079 2840 4181 2079
Size: 761 1341 2079
Fragments arranged by size:
2079 1341 761
BclI T'GATC_A
Cuts at: 845 1701 845
Size: 856 3325
BglI GCCn_nnn'nGGC
Cuts at: 619 619
Size: 4181
BglII A'GATC_T
Cuts at: 1696 3733 1696
Size: 2037 2144
BlpI GC'TnA_GC
Cuts at: 1293 1293
Size: 4181
Bme1580I G_kGCm'C
Cuts at: 420 459 687 1007 1806 1899 2955 420
Size: 39 228 320 799 93 1056 1646
Fragments arranged by size:
1646 1056 799 320 228 93 39
BmrI ACTGGGnnnn_n'
Cuts at: 1393 1474 1809 3953 1393
Size: 81 335 2144 1621
Fragments arranged by size:
2144 1621 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 753 2548 753
Size: 1795 2386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 723
Size: 4181
Bpu10I CC'TnA_GC
Cuts at: 301 625 823 973 301
Size: 324 198 150 3509
Fragments arranged by size:
3509 324 198 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1357 2236 2728 3026 1357
Size: 879 492 298 2512
Fragments arranged by size:
2512 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 118 3885 118
Size: 3767 414
BsaAI yAC'GTr
Cuts at: 1534 1534
Size: 4181
BsaBI GATnn'nnATC
Cuts at: 6 1695 3732 6
Size: 1689 2037 455
Fragments arranged by size:
2037 1689 455
BsaHI Gr'CG_yC
Cuts at: 245 338 1612 1860 2562 4058 245
Size: 93 1274 248 702 1496 368
Fragments arranged by size:
1496 1274 702 368 248 93
BsaWI w'CCGG_w
Cuts at: 1370 1891 2569 2843 2990 3401 1370
Size: 521 678 274 147 411 2150
Fragments arranged by size:
2150 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 192 222 507 537 192
Size: 30 285 30 3836
Fragments arranged by size:
3836 285 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 539 883 1055 539
Size: 344 172 3665
Fragments arranged by size:
3665 344 172
BseYI C'CCAG_C
Cuts at: 159 638 1606 2364 2941 3437 159
Size: 479 968 758 577 496 903
Fragments arranged by size:
968 903 758 577 496 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 481
Size: 4181
BsiEI CG_ry'CG
Cuts at: 104 1723 1769 2977 104
Size: 1619 46 1208 1308
Fragments arranged by size:
1619 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 420 570 1195 1973 2163 2955 420
Size: 150 625 778 190 792 1646
Fragments arranged by size:
1646 792 778 625 190 150
Bsp1286I G_dGCh'C
Cuts at: 420 459 545 570 687 1007 1195 1806 1899
Size: 39 86 25 117 320 188 611 93
Cuts at: 1899 1973 2163 2225 2955 420
Size: 74 190 62 730 1646
Fragments arranged by size:
1646 730 611 320 190 188 117 93
86 74 62 39 25
BspEI T'CCGG_A
Cuts at: 3401 3401
Size: 4181
BspHI T'CATG_A
Cuts at: 3357 3357
Size: 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 131 145 282 1231 1445 1747 2128 131
Size: 14 137 949 214 302 381 2184
Fragments arranged by size:
2184 949 381 302 214 137 14
BsrBI CCG'CTC
Cuts at: 50 2473 2527 4011 4176 50
Size: 2423 54 1484 165 55
Fragments arranged by size:
2423 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2093 2093
Size: 4181
BsrFI r'CCGG_y
Cuts at: 2179 2360 2569 2179
Size: 181 209 3791
Fragments arranged by size:
3791 209 181
BssSI C'ACGA_G
Cuts at: 835 2452 2810 835
Size: 1617 358 2206
Fragments arranged by size:
2206 1617 358
BstAPI GCAn_nnn'nTGC
Cuts at: 380 1143 1558 380
Size: 763 415 3003
Fragments arranged by size:
3003 763 415
BstBI TT'CG_AA
Cuts at: 2542 2542
Size: 4181
BstEII G'GTnAC_C
Cuts at: 132 132
Size: 4181
BstXI CCAn_nnnn'nTGG
Cuts at: 561 561
Size: 4181
BstYI r'GATC_y
Cuts at: 1 1113 1206 1673 1696 2031 2277 3289 3375
Size: 1112 93 467 23 335 246 1012 86
Cuts at: 3375 3387 3733 1
Size: 12 346 449
Fragments arranged by size:
1112 1012 467 449 346 335 246 93
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 862 862
Size: 4181
BtgI C'CryG_G
Cuts at: 107 440 779 107
Size: 333 339 3509
Fragments arranged by size:
3509 339 333
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2131 2312 2475 2131
Size: 181 163 3837
Fragments arranged by size:
3837 181 163
BtsI GCAGTG_nn'
Cuts at: 606 1424 606
Size: 818 3363
DraI TTT'AAA
Cuts at: 1180 1180
Size: 4181
DrdI GACnn_nn'nnGTC
Cuts at: 363 1887 2745 363
Size: 1524 858 1799
Fragments arranged by size:
1799 1524 858
EaeI y'GGCC_r
Cuts at: 270 1766 1940 2331 2358 3703 270
Size: 1496 174 391 27 1345 748
Fragments arranged by size:
1496 1345 748 391 174 27
EagI C'GGCC_G
Cuts at: 1766 1766
Size: 4181
EarI CTCTTCn'nnn_
Cuts at: 698 2204 2414 698
Size: 1506 210 2465
Fragments arranged by size:
2465 1506 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 895 1048 2389 2699 2845 3991 895
Size: 153 1341 310 146 1146 1085
Fragments arranged by size:
1341 1146 1085 310 153 146
EcoICRI GAG'CTC
Cuts at: 1193 1193
Size: 4181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 723 1001 2006 2438 3185 3453 461
Size: 262 278 1005 432 747 268 1189
Fragments arranged by size:
1189 1005 747 432 278 268 262
EcoNI CCTnn'n_nnAGG
Cuts at: 476 865 476
Size: 389 3792
EcoO109I rG'GnC_Cy
Cuts at: 143 455 456 575 1320 143
Size: 312 1 119 745 3004
Fragments arranged by size:
3004 745 312 119 1
EcoRI G'AATT_C
Cuts at: 1185 1185
Size: 4181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 4181
FspI TGC'GCA
Cuts at: 384 1962 384
Size: 1578 2603
HaeII r_GCGC'y
Cuts at: 625 844 1615 1863 2885 3864 625
Size: 219 771 248 1022 979 942
Fragments arranged by size:
1022 979 942 771 248 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 507 539 917 949 2023 2053 2055
Size: 32 186 32 378 32 1074 30 2
Cuts at: 2055 2085 289
Size: 30 2385
Fragments arranged by size:
2385 1074 378 186 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1220 1220
Size: 4181
KasI G'GCGC_C
Cuts at: 1611 1859 1611
Size: 248 3933
KpnI G_GTAC'C
Cuts at: 1201 1201
Size: 4181
MluI A'CGCG_T
Cuts at: 3614 3614
Size: 4181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1130 2852 3036 1130
Size: 1722 184 2275
Fragments arranged by size:
2275 1722 184
MscI TGG'CCA
Cuts at: 272 1942 272
Size: 1670 2511
MslI CAynn'nnrTG
Cuts at: 505 559 2170 2297 505
Size: 54 1611 127 2389
Fragments arranged by size:
2389 1611 127 54
MspA1I CmG'CkG
Cuts at: 761 798 827 1292 1606 1966 2979 3224 761
Size: 37 29 465 314 360 1013 245 1718
Fragments arranged by size:
1718 1013 465 360 314 245 37 29
NaeI GCC'GGC
Cuts at: 2362 2362
Size: 4181
NarI GG'CG_CC
Cuts at: 1612 1860 1612
Size: 248 3933
NcoI C'CATG_G
Cuts at: 107 440 779 107
Size: 333 339 3509
Fragments arranged by size:
3509 339 333
NgoMIV G'CCGG_C
Cuts at: 2360 2360
Size: 4181
NspI r_CATG'y
Cuts at: 1234 1734 2641 1234
Size: 500 907 2774
Fragments arranged by size:
2774 907 500
PasI CC'CwG_GG
Cuts at: 490 490
Size: 4181
PciI A'CATG_T
Cuts at: 2637 2637
Size: 4181
PflMI CCAn_nnn'nTGG
Cuts at: 780 780
Size: 4181
PfoI T'CCnGG_A
Cuts at: 332 332
Size: 4181
PmeI GTTT'AAAC
Cuts at: 1180 1180
Size: 4181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 3320
Size: 32 4149
PpuMI rG'GwC_Cy
Cuts at: 143 575 143
Size: 432 3749
PspOMI G'GGCC_C
Cuts at: 455 683 455
Size: 228 3953
PstI C_TGCA'G
Cuts at: 142 1228 1913 142
Size: 1086 685 2410
Fragments arranged by size:
2410 1086 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4181
PvuII CAG'CTG
Cuts at: 827 1606 1966 827
Size: 779 360 3042
Fragments arranged by size:
3042 779 360
RsrII CG'GwC_CG
Cuts at: 2376 2376
Size: 4181
SacI G_AGCT'C
Cuts at: 1195 1195
Size: 4181
SalI G'TCGA_C
Cuts at: 1218 1218
Size: 4181
SapI GCTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
SbfI CC_TGCA'GG
Cuts at: 142 1228 142
Size: 1086 3095
ScaI AGT'ACT
Cuts at: 3698 3698
Size: 4181
SfcI C'TryA_G
Cuts at: 33 138 580 1224 1909 2902 3093 33
Size: 105 442 644 685 993 191 1121
Fragments arranged by size:
1121 993 685 644 442 191 105
SfoI GGC'GCC
Cuts at: 1613 1861 1613
Size: 248 3933
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
SmaI CCC'GGG
Cuts at: 676 1203 676
Size: 527 3654
SmlI C'TyrA_G
Cuts at: 1336 2251 2743 3005 1336
Size: 915 492 262 2512
Fragments arranged by size:
2512 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4181
SphI G_CATG'C
Cuts at: 1234 1734 1234
Size: 500 3681
StyI C'CwwG_G
Cuts at: 107 341 440 779 1086 1117 1315 1669 107
Size: 234 99 339 307 31 198 354 2619
Fragments arranged by size:
2619 354 339 307 234 198 99 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 553 1764 1897 2356 2565 553
Size: 1211 133 459 209 2169
Fragments arranged by size:
2169 1211 459 209 133
TatI w'GTAC_w
Cuts at: 3696 3696
Size: 4181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 4149
Tth111I GACn'n_nGTC
Cuts at: 1978 1978
Size: 4181
XbaI T'CTAG_A
Cuts at: 1212 1212
Size: 4181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 438 1676 114
Size: 324 1238 2619
Fragments arranged by size:
2619 1238 324
XmaI C'CCGG_G
Cuts at: 674 1201 674
Size: 527 3654
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI