Restriction map for
pF1KB7222
(Circular) (Six-base) MAPSORT of: pF1KB7222.seq Check: 2740 from: 1 to: 4478
pF1KB7222 4478 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 990 990
Size: 4478
AatII G_ACGT'C
Cuts at: 1265 1265
Size: 4478
AccI GT'mk_AC
Cuts at: 1516 1516
Size: 4478
Acc65I G'GTAC_C
Cuts at: 1494 1494
Size: 4478
AclI AA'CG_TT
Cuts at: 4318 4318
Size: 4478
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 636 799 820 980 2303 2735 3482 3750 636
Size: 163 21 160 1323 432 747 268 1364
Fragments arranged by size:
1364 1323 747 432 268 163 160 21
AflIII A'CryG_T
Cuts at: 524 1292 1830 2934 3911 524
Size: 768 538 1104 977 1091
Fragments arranged by size:
1104 1091 977 768 538
AgeI A'CCGG_T
Cuts at: 2866 2866
Size: 4478
AhdI GACnn_n'nnGTC
Cuts at: 540 1063 540
Size: 523 3955
AleI CACnn'nnGTG
Cuts at: 187 1189 187
Size: 1002 3476
AlwNI CAG_nnn'CTG
Cuts at: 1254 1783 3350 1254
Size: 529 1567 2382
Fragments arranged by size:
2382 1567 529
ApaI G_GGCC'C
Cuts at: 1272 1272
Size: 4478
ApaLI G'TGCA_C
Cuts at: 1346 3248 1346
Size: 1902 2576
ApoI r'AATT_y
Cuts at: 1482 3831 4087 1482
Size: 2349 256 1873
Fragments arranged by size:
2349 1873 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4478
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4478
AvaI C'yCGr_G
Cuts at: 1498 1498
Size: 4478
BamHI G'GATC_C
Cuts at: 722 1503 722
Size: 781 3697
BanI G'GyrC_C
Cuts at: 917 1494 1908 2156 2191 917
Size: 577 414 248 35 3204
Fragments arranged by size:
3204 577 414 248 35
BanII G_rGCy'C
Cuts at: 160 181 569 1272 1492 2522 160
Size: 21 388 703 220 1030 2116
Fragments arranged by size:
2116 1030 703 388 220 21
BbeI G_GCGC'C
Cuts at: 1912 2160 1912
Size: 248 4230
BbsI GAAGACnn'nnnn_
Cuts at: 583 968 1378 583
Size: 385 410 3683
Fragments arranged by size:
3683 410 385
BciVI GTATCCnnnnn_n'
Cuts at: 2376 3137 4478 2376
Size: 761 1341 2376
Fragments arranged by size:
2376 1341 761
BclI T'GATC_A
Cuts at: 1181 1998 1181
Size: 817 3661
BglI GCCn_nnn'nGGC
Cuts at: 434 637 1204 434
Size: 203 567 3708
Fragments arranged by size:
3708 567 203
BglII A'GATC_T
Cuts at: 1993 4030 1993
Size: 2037 2441
BlpI GC'TnA_GC
Cuts at: 1094 1590 1094
Size: 496 3982
Bme1580I G_kGCm'C
Cuts at: 532 1272 1350 1454 2103 2196 3252 532
Size: 740 78 104 649 93 1056 1758
Fragments arranged by size:
1758 1056 740 649 104 93 78
BmrI ACTGGGnnnn_n'
Cuts at: 1690 1771 2106 4250 1690
Size: 81 335 2144 1918
Fragments arranged by size:
2144 1918 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2845 2845
Size: 4478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 350 463 481 502 553 778 925 1096
Size: 161 113 18 21 51 225 147 171
Cuts at: 1096 1246 1297 1330 189
Size: 150 51 33 3337
Fragments arranged by size:
3337 225 171 161 150 147 113 51
51 33 21 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 136 1654 2533 3025 3323 109
Size: 27 1518 879 492 298 1264
Fragments arranged by size:
1518 1264 879 492 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 152 397 4182 152
Size: 245 3785 448
Fragments arranged by size:
3785 448 245
BsaAI yAC'GTr
Cuts at: 1295 1831 1295
Size: 536 3942
BsaBI GATnn'nnATC
Cuts at: 6 1992 4029 6
Size: 1986 2037 455
Fragments arranged by size:
2037 1986 455
BsaHI Gr'CG_yC
Cuts at: 1262 1909 2157 2859 4355 1262
Size: 647 248 702 1496 1385
Fragments arranged by size:
1496 1385 702 647 248
BsaWI w'CCGG_w
Cuts at: 551 1667 2188 2866 3140 3287 3698 551
Size: 1116 521 678 274 147 411 1331
Fragments arranged by size:
1331 1116 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 901 931 1348 1378 901
Size: 30 417 30 4001
Fragments arranged by size:
4001 417 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 577 799 829 922 1000 577
Size: 222 30 93 78 4055
Fragments arranged by size:
4055 222 93 78 30
BseYI C'CCAG_C
Cuts at: 1157 1199 1903 2661 3238 3734 1157
Size: 42 704 758 577 496 1901
Fragments arranged by size:
1901 758 704 577 496 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1433 1433
Size: 4478
BsiEI CG_ry'CG
Cuts at: 104 426 2020 2066 3274 104
Size: 322 1594 46 1208 1308
Fragments arranged by size:
1594 1308 1208 322 46
BsiHKAI G_wGCw'C
Cuts at: 569 1350 1492 2270 2460 3252 569
Size: 781 142 778 190 792 1795
Fragments arranged by size:
1795 792 781 778 190 142
BsmI GAATG_Cn'
Cuts at: 606 606
Size: 4478
BsmBI CGTCTCn'nnnn_
Cuts at: 661 1050 1129 661
Size: 389 79 4010
Fragments arranged by size:
4010 389 79
Bsp1286I G_dGCh'C
Cuts at: 160 181 532 569 1272 1350 1454 1492 2103
Size: 21 351 37 703 78 104 38 611
Cuts at: 2103 2196 2270 2460 2522 3252 160
Size: 93 74 190 62 730 1386
Fragments arranged by size:
1386 730 703 611 351 190 104 93
78 74 62 38 37 21
BspEI T'CCGG_A
Cuts at: 551 3698 551
Size: 3147 1331
BspHI T'CATG_A
Cuts at: 3654 3654
Size: 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 504 990 1528 1742 2044 2425 504
Size: 486 538 214 302 381 2557
Fragments arranged by size:
2557 538 486 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1230 2770 2824 4308 4473 50
Size: 1180 1540 54 1484 165 55
Fragments arranged by size:
1540 1484 1180 165 55 54
BsrDI GCAATG_nn'
Cuts at: 203 399 2390 203
Size: 196 1991 2291
Fragments arranged by size:
2291 1991 196
BsrFI r'CCGG_y
Cuts at: 130 433 2476 2657 2866 130
Size: 303 2043 181 209 1742
Fragments arranged by size:
2043 1742 303 209 181
BssSI C'ACGA_G
Cuts at: 185 2749 3107 185
Size: 2564 358 1556
Fragments arranged by size:
2564 1556 358
BstAPI GCAn_nnn'nTGC
Cuts at: 215 1427 1855 215
Size: 1212 428 2838
Fragments arranged by size:
2838 1212 428
BstBI TT'CG_AA
Cuts at: 2839 2839
Size: 4478
BstEII G'GTnAC_C
Cuts at: 847 847
Size: 4478
BstXI CCAn_nnnn'nTGG
Cuts at: 612 612
Size: 4478
BstYI r'GATC_y
Cuts at: 1 722 1211 1503 1970 1993 2328 2574 3586
Size: 721 489 292 467 23 335 246 1012
Cuts at: 3586 3672 3684 4030 1
Size: 86 12 346 449
Fragments arranged by size:
1012 721 489 467 449 346 335 292
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4478
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2428 2609 2772 2428
Size: 181 163 4134
Fragments arranged by size:
4134 181 163
BtsI GCAGTG_nn'
Cuts at: 829 1180 1721 829
Size: 351 541 3586
Fragments arranged by size:
3586 541 351
DraI TTT'AAA
Cuts at: 1477 1477
Size: 4478
DrdI GACnn_nn'nnGTC
Cuts at: 675 2184 3042 675
Size: 1509 858 2111
Fragments arranged by size:
2111 1509 858
EaeI y'GGCC_r
Cuts at: 242 431 1205 2063 2237 2628 2655 4000 242
Size: 189 774 858 174 391 27 1345 720
Fragments arranged by size:
1345 858 774 720 391 189 174 27
EagI C'GGCC_G
Cuts at: 2063 2063
Size: 4478
EarI CTCTTCn'nnn_
Cuts at: 144 2501 2711 144
Size: 2357 210 1911
Fragments arranged by size:
2357 1911 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2686 2996 3142 4288 2686
Size: 310 146 1146 2876
Fragments arranged by size:
2876 1146 310 146
EcoICRI GAG'CTC
Cuts at: 567 1490 567
Size: 923 3555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 350 463 481 502 553 636 778 799
Size: 161 113 18 21 51 83 142 21
Cuts at: 799 820 925 980 1096 1246 1297 1330 2303
Size: 21 105 55 116 150 51 33 973
Cuts at: 2303 2735 3482 3750 189
Size: 432 747 268 917
Fragments arranged by size:
973 917 747 432 268 161 150 142
116 113 105 83 55 51 51 33
21 21 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 329 445 781 329
Size: 116 336 4026
Fragments arranged by size:
4026 336 116
EcoO109I rG'GnC_Cy
Cuts at: 516 1302 1617 516
Size: 786 315 3377
Fragments arranged by size:
3377 786 315
EcoRI G'AATT_C
Cuts at: 1482 1482
Size: 4478
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 150 2497 150
Size: 2347 2131
FspI TGC'GCA
Cuts at: 718 2259 718
Size: 1541 2937
HaeII r_GCGC'y
Cuts at: 1912 2160 3182 4161 1912
Size: 248 1022 979 2229
Fragments arranged by size:
2229 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 526 558 1049 1081 2320 2350 2352 2382 526
Size: 32 491 32 1239 30 2 30 2622
Fragments arranged by size:
2622 1239 491 32 32 30 30 2
HincII GTy'rAC
Cuts at: 888 1068 1517 888
Size: 180 449 3849
Fragments arranged by size:
3849 449 180
HindIII A'AGCT_T
Cuts at: 220 220
Size: 4478
KasI G'GCGC_C
Cuts at: 1908 2156 1908
Size: 248 4230
KpnI G_GTAC'C
Cuts at: 1498 1498
Size: 4478
MluI A'CGCG_T
Cuts at: 3911 3911
Size: 4478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 950 3149 3333 950
Size: 2199 184 2095
Fragments arranged by size:
2199 2095 184
MscI TGG'CCA
Cuts at: 244 2239 244
Size: 1995 2483
MslI CAynn'nnrTG
Cuts at: 187 1189 1447 2467 2594 187
Size: 1002 258 1020 127 2071
Fragments arranged by size:
2071 1020 1002 258 127
MspA1I CmG'CkG
Cuts at: 302 459 690 1161 1203 1589 1903 2263 3276
Size: 157 231 471 42 386 314 360 1013
Cuts at: 3276 3521 302
Size: 245 1259
Fragments arranged by size:
1259 1013 471 386 360 314 245 231
157 42
NaeI GCC'GGC
Cuts at: 132 435 2659 132
Size: 303 2224 1951
Fragments arranged by size:
2224 1951 303
NarI GG'CG_CC
Cuts at: 1909 2157 1909
Size: 248 4230
NcoI C'CATG_G
Cuts at: 107 107
Size: 4478
NgoMIV G'CCGG_C
Cuts at: 130 433 2657 130
Size: 303 2224 1951
Fragments arranged by size:
2224 1951 303
NspI r_CATG'y
Cuts at: 528 1531 2031 2938 528
Size: 1003 500 907 2068
Fragments arranged by size:
2068 1003 907 500
PasI CC'CwG_GG
Cuts at: 1459 1459
Size: 4478
PciI A'CATG_T
Cuts at: 524 2934 524
Size: 2410 2068
PflMI CCAn_nnn'nTGG
Cuts at: 1002 1002
Size: 4478
PfoI T'CCnGG_A
Cuts at: 725 725
Size: 4478
PmeI GTTT'AAAC
Cuts at: 1477 1477
Size: 4478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 3617
Size: 32 4446
PpuMI rG'GwC_Cy
Cuts at: 516 516
Size: 4478
PspOMI G'GGCC_C
Cuts at: 1268 1268
Size: 4478
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1228 1260 1228
Size: 32 4446
PstI C_TGCA'G
Cuts at: 645 1091 1432 1525 2210 645
Size: 446 341 93 685 2913
Fragments arranged by size:
2913 685 446 341 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4478
PvuII CAG'CTG
Cuts at: 302 459 690 1161 1203 1903 2263 302
Size: 157 231 471 42 700 360 2517
Fragments arranged by size:
2517 700 471 360 231 157 42
RsrII CG'GwC_CG
Cuts at: 2673 2673
Size: 4478
SacI G_AGCT'C
Cuts at: 569 1492 569
Size: 923 3555
SalI G'TCGA_C
Cuts at: 1515 1515
Size: 4478
SapI GCTCTTCn'nnn_
Cuts at: 2501 2711 2501
Size: 210 4268
SbfI CC_TGCA'GG
Cuts at: 1525 1525
Size: 4478
ScaI AGT'ACT
Cuts at: 3995 3995
Size: 4478
SexAI A'CCwGG_T
Cuts at: 1247 1247
Size: 4478
SfcI C'TryA_G
Cuts at: 33 288 348 641 1087 1149 1428 1521 2206
Size: 255 60 293 446 62 279 93 685
Cuts at: 2206 3199 3390 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 446 293 279 255 191
93 62 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 1204 1204
Size: 4478
SfoI GGC'GCC
Cuts at: 1910 2158 1910
Size: 248 4230
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4478
SmaI CCC'GGG
Cuts at: 1500 1500
Size: 4478
SmlI C'TyrA_G
Cuts at: 124 151 1633 2548 3040 3302 124
Size: 27 1482 915 492 262 1300
Fragments arranged by size:
1482 1300 915 492 262 27
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4478
SphI G_CATG'C
Cuts at: 1531 2031 1531
Size: 500 3978
StyI C'CwwG_G
Cuts at: 107 1612 1966 107
Size: 1505 354 2619
Fragments arranged by size:
2619 1505 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 991 2061 2194 2653 2862 991
Size: 1070 133 459 209 2607
Fragments arranged by size:
2607 1070 459 209 133
TatI w'GTAC_w
Cuts at: 200 3993 200
Size: 3793 685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 901 933 3995 4027 901
Size: 32 3062 32 1352
Fragments arranged by size:
3062 1352 32 32
Tth111I GACn'n_nGTC
Cuts at: 2275 2275
Size: 4478
XbaI T'CTAG_A
Cuts at: 1509 1509
Size: 4478
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 612 1973 612
Size: 1361 3117
XmaI C'CCGG_G
Cuts at: 1498 1498
Size: 4478
ZraI GAC'GTC
Cuts at: 1263 1263
Size: 4478
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI SacII SanDI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI