Restriction map for
pF1KB7220
(Circular) (Six-base) MAPSORT of: pF1KB7220.seq Check: 3552 from: 1 to: 4070
pF1KB7220 4070 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1108 1108
Size: 4070
Acc65I G'GTAC_C
Cuts at: 1086 1086
Size: 4070
AclI AA'CG_TT
Cuts at: 3910 3910
Size: 4070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1895 2327 3074 3342 682
Size: 1213 432 747 268 1410
Fragments arranged by size:
1410 1213 747 432 268
AflIII A'CryG_T
Cuts at: 1422 2526 3503 1422
Size: 1104 977 1989
Fragments arranged by size:
1989 1104 977
AgeI A'CCGG_T
Cuts at: 2458 2458
Size: 4070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 447 447
Size: 4070
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 524 556 524
Size: 32 4038
AlwNI CAG_nnn'CTG
Cuts at: 1375 2942 1375
Size: 1567 2503
ApaLI G'TGCA_C
Cuts at: 2840 2840
Size: 4070
ApoI r'AATT_y
Cuts at: 626 1074 3423 3679 626
Size: 448 2349 256 1017
Fragments arranged by size:
2349 1017 448 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4070
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
AvaI C'yCGr_G
Cuts at: 1090 1090
Size: 4070
BamHI G'GATC_C
Cuts at: 1095 1095
Size: 4070
BanI G'GyrC_C
Cuts at: 1086 1500 1748 1783 1086
Size: 414 248 35 3373
Fragments arranged by size:
3373 414 248 35
BanII G_rGCy'C
Cuts at: 1084 2114 1084
Size: 1030 3040
BbeI G_GCGC'C
Cuts at: 1504 1752 1504
Size: 248 3822
BciVI GTATCCnnnnn_n'
Cuts at: 1968 2729 4070 1968
Size: 761 1341 1968
Fragments arranged by size:
1968 1341 761
BclI T'GATC_A
Cuts at: 380 1590 380
Size: 1210 2860
BglII A'GATC_T
Cuts at: 1585 3622 1585
Size: 2037 2033
BlpI GC'TnA_GC
Cuts at: 1055 1182 1055
Size: 127 3943
Bme1580I G_kGCm'C
Cuts at: 1695 1788 2844 1695
Size: 93 1056 2921
Fragments arranged by size:
2921 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1015 1282 1363 1698 3842 1015
Size: 267 81 335 2144 1243
Fragments arranged by size:
2144 1243 335 267 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 4070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1246 2125 2617 2915 601
Size: 645 879 492 298 1756
Fragments arranged by size:
1756 879 645 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 577 3774 577
Size: 3197 873
BsaAI yAC'GTr
Cuts at: 1423 1423
Size: 4070
BsaBI GATnn'nnATC
Cuts at: 6 1584 3621 6
Size: 1578 2037 455
Fragments arranged by size:
2037 1578 455
BsaHI Gr'CG_yC
Cuts at: 1501 1749 2451 3947 1501
Size: 248 702 1496 1624
Fragments arranged by size:
1624 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1259 1780 2458 2732 2879 3290 1259
Size: 521 678 274 147 411 2039
Fragments arranged by size:
2039 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 175 765 1006 1009 136
Size: 39 590 241 3 3197
Fragments arranged by size:
3197 590 241 39 3
BseYI C'CCAG_C
Cuts at: 1495 2253 2830 3326 1495
Size: 758 577 496 2239
Fragments arranged by size:
2239 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 597 481
Size: 116 3954
BsiEI CG_ry'CG
Cuts at: 104 1612 1658 2866 104
Size: 1508 46 1208 1308
Fragments arranged by size:
1508 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1084 1862 2052 2844 1084
Size: 778 190 792 2310
Fragments arranged by size:
2310 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1037 1037
Size: 4070
Bsp1286I G_dGCh'C
Cuts at: 1084 1695 1788 1862 2052 2114 2844 1084
Size: 611 93 74 190 62 730 2310
Fragments arranged by size:
2310 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3290 3290
Size: 4070
BspHI T'CATG_A
Cuts at: 512 3246 512
Size: 2734 1336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 1120 1334 1636 2017 242
Size: 878 214 302 381 2295
Fragments arranged by size:
2295 878 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2362 2416 3900 4065 50
Size: 2312 54 1484 165 55
Fragments arranged by size:
2312 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 419 1049 1982 419
Size: 630 933 2507
Fragments arranged by size:
2507 933 630
BsrFI r'CCGG_y
Cuts at: 2068 2249 2458 2068
Size: 181 209 3680
Fragments arranged by size:
3680 209 181
BsrGI T'GTAC_A
Cuts at: 935 935
Size: 4070
BssSI C'ACGA_G
Cuts at: 2341 2699 2341
Size: 358 3712
BstAPI GCAn_nnn'nTGC
Cuts at: 1447 1447
Size: 4070
BstBI TT'CG_AA
Cuts at: 2431 2431
Size: 4070
BstEII G'GTnAC_C
Cuts at: 216 945 216
Size: 729 3341
BstXI CCAn_nnnn'nTGG
Cuts at: 213 723 213
Size: 510 3560
BstYI r'GATC_y
Cuts at: 1 1095 1562 1585 1920 2166 3178 3264 3276
Size: 1094 467 23 335 246 1012 86 12
Cuts at: 3276 3622 1
Size: 346 449
Fragments arranged by size:
1094 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 979 1021 979
Size: 42 4028
BtgI C'CryG_G
Cuts at: 107 189 941 107
Size: 82 752 3236
Fragments arranged by size:
3236 752 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2020 2201 2364 2020
Size: 181 163 3726
Fragments arranged by size:
3726 181 163
BtsI GCAGTG_nn'
Cuts at: 1313 1313
Size: 4070
DraI TTT'AAA
Cuts at: 1069 1069
Size: 4070
DrdI GACnn_nn'nnGTC
Cuts at: 1776 2634 1776
Size: 858 3212
EaeI y'GGCC_r
Cuts at: 479 1655 1829 2220 2247 3592 479
Size: 1176 174 391 27 1345 957
Fragments arranged by size:
1345 1176 957 391 174 27
EagI C'GGCC_G
Cuts at: 1655 1655
Size: 4070
EarI CTCTTCn'nnn_
Cuts at: 2093 2303 2093
Size: 210 3860
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2278 2588 2734 3880 2278
Size: 310 146 1146 2468
Fragments arranged by size:
2468 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1082 1082
Size: 4070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1895 2327 3074 3342 682
Size: 1213 432 747 268 1410
Fragments arranged by size:
1410 1213 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 548 1209 548
Size: 661 3409
EcoRI G'AATT_C
Cuts at: 1074 1074
Size: 4070
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 4070
FspI TGC'GCA
Cuts at: 1851 1851
Size: 4070
HaeII r_GCGC'y
Cuts at: 1504 1752 2774 3753 1504
Size: 248 1022 979 1821
Fragments arranged by size:
1821 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1004 1036 1912 1942 1944 1974 1004
Size: 32 876 30 2 30 3100
Fragments arranged by size:
3100 876 32 30 30 2
HincII GTy'rAC
Cuts at: 1109 1109
Size: 4070
KasI G'GCGC_C
Cuts at: 1500 1748 1500
Size: 248 3822
KpnI G_GTAC'C
Cuts at: 1090 1090
Size: 4070
MluI A'CGCG_T
Cuts at: 3503 3503
Size: 4070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 114 323 2741 2925 114
Size: 209 2418 184 1259
Fragments arranged by size:
2418 1259 209 184
MscI TGG'CCA
Cuts at: 481 1831 481
Size: 1350 2720
MslI CAynn'nnrTG
Cuts at: 280 429 619 700 952 2059 2186 280
Size: 149 190 81 252 1107 127 2164
Fragments arranged by size:
2164 1107 252 190 149 127 81
MspA1I CmG'CkG
Cuts at: 1181 1495 1855 2868 3113 1181
Size: 314 360 1013 245 2138
Fragments arranged by size:
2138 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2251 2251
Size: 4070
NarI GG'CG_CC
Cuts at: 1501 1749 1501
Size: 248 3822
NcoI C'CATG_G
Cuts at: 107 189 941 107
Size: 82 752 3236
Fragments arranged by size:
3236 752 82
NgoMIV G'CCGG_C
Cuts at: 2249 2249
Size: 4070
NspI r_CATG'y
Cuts at: 177 1123 1623 2530 177
Size: 946 500 907 1717
Fragments arranged by size:
1717 946 907 500
PciI A'CATG_T
Cuts at: 2526 2526
Size: 4070
PmeI GTTT'AAAC
Cuts at: 1069 1069
Size: 4070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 4038
PpuMI rG'GwC_Cy
Cuts at: 548 548
Size: 4070
PshAI GACnn'nnGTC
Cuts at: 718 718
Size: 4070
PstI C_TGCA'G
Cuts at: 894 1117 1802 894
Size: 223 685 3162
Fragments arranged by size:
3162 685 223
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4070
PvuII CAG'CTG
Cuts at: 1495 1855 1495
Size: 360 3710
RsrII CG'GwC_CG
Cuts at: 2265 2265
Size: 4070
SacI G_AGCT'C
Cuts at: 1084 1084
Size: 4070
SalI G'TCGA_C
Cuts at: 1107 1107
Size: 4070
SapI GCTCTTCn'nnn_
Cuts at: 2093 2303 2093
Size: 210 3860
SbfI CC_TGCA'GG
Cuts at: 1117 1117
Size: 4070
ScaI AGT'ACT
Cuts at: 3587 3587
Size: 4070
SexAI A'CCwGG_T
Cuts at: 212 212
Size: 4070
SfcI C'TryA_G
Cuts at: 33 890 972 1113 1798 2791 2982 33
Size: 857 82 141 685 993 191 1121
Fragments arranged by size:
1121 993 857 685 191 141 82
SfoI GGC'GCC
Cuts at: 1502 1750 1502
Size: 248 3822
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
SmaI CCC'GGG
Cuts at: 1092 1092
Size: 4070
SmlI C'TyrA_G
Cuts at: 580 1225 2140 2632 2894 580
Size: 645 915 492 262 1756
Fragments arranged by size:
1756 915 645 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4070
SphI G_CATG'C
Cuts at: 1123 1623 1123
Size: 500 3570
StyI C'CwwG_G
Cuts at: 107 189 870 941 1204 1558 107
Size: 82 681 71 263 354 2619
Fragments arranged by size:
2619 681 354 263 82 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 715 1653 1786 2245 2454 715
Size: 938 133 459 209 2331
Fragments arranged by size:
2331 938 459 209 133
TatI w'GTAC_w
Cuts at: 284 935 3585 284
Size: 651 2650 769
Fragments arranged by size:
2650 769 651
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 4038
Tth111I GACn'n_nGTC
Cuts at: 1867 1867
Size: 4070
XbaI T'CTAG_A
Cuts at: 1101 1101
Size: 4070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 130 1565 130
Size: 1435 2635
XmaI C'CCGG_G
Cuts at: 1090 1090
Size: 4070
XmnI GAAnn'nnTTC
Cuts at: 964 964
Size: 4070
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BssHII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI