Restriction map for
pF1KB7219
(Circular) (Six-base) MAPSORT of: pF1KB7219.seq Check: 3242 from: 1 to: 4073
pF1KB7219 4073 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 278 835 278
Size: 557 3516
AccI GT'mk_AC
Cuts at: 133 1111 133
Size: 978 3095
Acc65I G'GTAC_C
Cuts at: 1089 1089
Size: 4073
AclI AA'CG_TT
Cuts at: 3913 3913
Size: 4073
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 821 1898 2330 3077 3345 821
Size: 1077 432 747 268 1549
Fragments arranged by size:
1549 1077 747 432 268
AflIII A'CryG_T
Cuts at: 1425 2529 3506 1425
Size: 1104 977 1992
Fragments arranged by size:
1992 1104 977
AgeI A'CCGG_T
Cuts at: 2461 2461
Size: 4073
AlwNI CAG_nnn'CTG
Cuts at: 1378 2945 1378
Size: 1567 2506
ApaLI G'TGCA_C
Cuts at: 2843 2843
Size: 4073
ApoI r'AATT_y
Cuts at: 139 647 1077 3426 3682 139
Size: 508 430 2349 256 530
Fragments arranged by size:
2349 530 508 430 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4073
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
AvaI C'yCGr_G
Cuts at: 1093 1093
Size: 4073
BamHI G'GATC_C
Cuts at: 1098 1098
Size: 4073
BanI G'GyrC_C
Cuts at: 523 1089 1503 1751 1786 523
Size: 566 414 248 35 2810
Fragments arranged by size:
2810 566 414 248 35
BanII G_rGCy'C
Cuts at: 1087 2117 1087
Size: 1030 3043
BbeI G_GCGC'C
Cuts at: 1507 1755 1507
Size: 248 3825
BbsI GAAGACnn'nnnn_
Cuts at: 932 932
Size: 4073
BciVI GTATCCnnnnn_n'
Cuts at: 1971 2732 4073 1971
Size: 761 1341 1971
Fragments arranged by size:
1971 1341 761
BclI T'GATC_A
Cuts at: 1593 1593
Size: 4073
BglI GCCn_nnn'nGGC
Cuts at: 430 532 430
Size: 102 3971
BglII A'GATC_T
Cuts at: 1588 3625 1588
Size: 2037 2036
BlpI GC'TnA_GC
Cuts at: 831 1185 831
Size: 354 3719
Bme1580I G_kGCm'C
Cuts at: 1698 1791 2847 1698
Size: 93 1056 2924
Fragments arranged by size:
2924 1056 93
BmgBI CAC'GTC
Cuts at: 131 131
Size: 4073
BmrI ACTGGGnnnn_n'
Cuts at: 569 1285 1366 1701 3845 569
Size: 716 81 335 2144 797
Fragments arranged by size:
2144 797 716 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 160
Size: 4073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 2128 2620 2918 1249
Size: 879 492 298 2404
Fragments arranged by size:
2404 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3777 3777
Size: 4073
BsaAI yAC'GTr
Cuts at: 1426 1426
Size: 4073
BsaBI GATnn'nnATC
Cuts at: 6 1587 3624 6
Size: 1581 2037 455
Fragments arranged by size:
2037 1581 455
BsaHI Gr'CG_yC
Cuts at: 1504 1752 2454 3950 1504
Size: 248 702 1496 1627
Fragments arranged by size:
1627 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1262 1783 2461 2735 2882 3293 1262
Size: 521 678 274 147 411 2042
Fragments arranged by size:
2042 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 544 1498 2256 2833 3329 544
Size: 954 758 577 496 1288
Fragments arranged by size:
1288 954 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 601 256
Size: 345 3728
BsiEI CG_ry'CG
Cuts at: 104 1615 1661 2869 104
Size: 1511 46 1208 1308
Fragments arranged by size:
1511 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1087 1865 2055 2847 1087
Size: 778 190 792 2313
Fragments arranged by size:
2313 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1087 1698 1791 1865 2055 2117 2847 1087
Size: 611 93 74 190 62 730 2313
Fragments arranged by size:
2313 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3293 3293
Size: 4073
BspHI T'CATG_A
Cuts at: 512 3249 512
Size: 2737 1336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 835 1123 1337 1639 2020 278
Size: 557 288 214 302 381 2331
Fragments arranged by size:
2331 557 381 302 288 214
BsrBI CCG'CTC
Cuts at: 50 2365 2419 3903 4068 50
Size: 2315 54 1484 165 55
Fragments arranged by size:
2315 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 602 1985 602
Size: 1383 2690
BsrFI r'CCGG_y
Cuts at: 2071 2252 2461 2071
Size: 181 209 3683
Fragments arranged by size:
3683 209 181
BssSI C'ACGA_G
Cuts at: 2344 2702 2344
Size: 358 3715
BstAPI GCAn_nnn'nTGC
Cuts at: 1450 1450
Size: 4073
BstBI TT'CG_AA
Cuts at: 2434 2434
Size: 4073
BstXI CCAn_nnnn'nTGG
Cuts at: 363 363
Size: 4073
BstYI r'GATC_y
Cuts at: 1 1098 1565 1588 1923 2169 3181 3267 3279
Size: 1097 467 23 335 246 1012 86 12
Cuts at: 3279 3625 1
Size: 346 449
Fragments arranged by size:
1097 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 425 434 536 107
Size: 318 9 102 3644
Fragments arranged by size:
3644 318 102 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2023 2204 2367 2023
Size: 181 163 3729
Fragments arranged by size:
3729 181 163
BtsI GCAGTG_nn'
Cuts at: 1316 1316
Size: 4073
DraI TTT'AAA
Cuts at: 1072 1072
Size: 4073
DraIII CAC_nnn'GTG
Cuts at: 440 440
Size: 4073
DrdI GACnn_nn'nnGTC
Cuts at: 1779 2637 1779
Size: 858 3215
EaeI y'GGCC_r
Cuts at: 479 533 1658 1832 2223 2250 3595 479
Size: 54 1125 174 391 27 1345 957
Fragments arranged by size:
1345 1125 957 391 174 54 27
EagI C'GGCC_G
Cuts at: 1658 1658
Size: 4073
EarI CTCTTCn'nnn_
Cuts at: 2096 2306 2096
Size: 210 3863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2281 2591 2737 3883 2281
Size: 310 146 1146 2471
Fragments arranged by size:
2471 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1085 1085
Size: 4073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 821 1898 2330 3077 3345 160
Size: 661 1077 432 747 268 888
Fragments arranged by size:
1077 888 747 661 432 268
EcoO109I rG'GnC_Cy
Cuts at: 548 939 1212 548
Size: 391 273 3409
Fragments arranged by size:
3409 391 273
EcoRI G'AATT_C
Cuts at: 1077 1077
Size: 4073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 402 2092 402
Size: 1690 2383
FspI TGC'GCA
Cuts at: 1854 1854
Size: 4073
HaeII r_GCGC'y
Cuts at: 1507 1755 2777 3756 1507
Size: 248 1022 979 1824
Fragments arranged by size:
1824 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 115 147 227 259 491 523 1915 1945 1947
Size: 32 80 32 232 32 1392 30 2
Cuts at: 1947 1977 115
Size: 30 2211
Fragments arranged by size:
2211 1392 232 80 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 134 1112 134
Size: 978 3095
KasI G'GCGC_C
Cuts at: 1503 1751 1503
Size: 248 3825
KpnI G_GTAC'C
Cuts at: 1093 1093
Size: 4073
MluI A'CGCG_T
Cuts at: 3506 3506
Size: 4073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2744 2928 2744
Size: 184 3889
MscI TGG'CCA
Cuts at: 481 535 1834 481
Size: 54 1299 2720
Fragments arranged by size:
2720 1299 54
MslI CAynn'nnrTG
Cuts at: 280 2062 2189 280
Size: 1782 127 2164
Fragments arranged by size:
2164 1782 127
MspA1I CmG'CkG
Cuts at: 544 1184 1498 1858 2871 3116 544
Size: 640 314 360 1013 245 1501
Fragments arranged by size:
1501 1013 640 360 314 245
NaeI GCC'GGC
Cuts at: 2254 2254
Size: 4073
NarI GG'CG_CC
Cuts at: 1504 1752 1504
Size: 248 3825
NcoI C'CATG_G
Cuts at: 107 425 536 107
Size: 318 111 3644
Fragments arranged by size:
3644 318 111
NgoMIV G'CCGG_C
Cuts at: 2252 2252
Size: 4073
NspI r_CATG'y
Cuts at: 1126 1626 2533 1126
Size: 500 907 2666
Fragments arranged by size:
2666 907 500
PciI A'CATG_T
Cuts at: 2529 2529
Size: 4073
PmeI GTTT'AAAC
Cuts at: 1072 1072
Size: 4073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 4041
PpuMI rG'GwC_Cy
Cuts at: 548 548
Size: 4073
PstI C_TGCA'G
Cuts at: 1120 1805 1120
Size: 685 3388
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4073
PvuII CAG'CTG
Cuts at: 544 1498 1858 544
Size: 954 360 2759
Fragments arranged by size:
2759 954 360
RsrII CG'GwC_CG
Cuts at: 2268 2268
Size: 4073
SacI G_AGCT'C
Cuts at: 1087 1087
Size: 4073
SalI G'TCGA_C
Cuts at: 132 1110 132
Size: 978 3095
SapI GCTCTTCn'nnn_
Cuts at: 2096 2306 2096
Size: 210 3863
SbfI CC_TGCA'GG
Cuts at: 1120 1120
Size: 4073
ScaI AGT'ACT
Cuts at: 3590 3590
Size: 4073
SfcI C'TryA_G
Cuts at: 33 993 1116 1801 2794 2985 33
Size: 960 123 685 993 191 1121
Fragments arranged by size:
1121 993 960 685 191 123
SfoI GGC'GCC
Cuts at: 1505 1753 1505
Size: 248 3825
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
SmaI CCC'GGG
Cuts at: 1095 1095
Size: 4073
SmlI C'TyrA_G
Cuts at: 1228 2143 2635 2897 1228
Size: 915 492 262 2404
Fragments arranged by size:
2404 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4073
SphI G_CATG'C
Cuts at: 1126 1626 1126
Size: 500 3573
StuI AGG'CCT
Cuts at: 817 817
Size: 4073
StyI C'CwwG_G
Cuts at: 107 425 536 1207 1561 107
Size: 318 111 671 354 2619
Fragments arranged by size:
2619 671 354 318 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 182 383 1656 1789 2248 2457 182
Size: 201 1273 133 459 209 1798
Fragments arranged by size:
1798 1273 459 209 201 133
TatI w'GTAC_w
Cuts at: 3588 3588
Size: 4073
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3590 3622 3590
Size: 32 4041
Tth111I GACn'n_nGTC
Cuts at: 708 1870 708
Size: 1162 2911
XbaI T'CTAG_A
Cuts at: 1104 1104
Size: 4073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1568 1568
Size: 4073
XmaI C'CCGG_G
Cuts at: 1093 1093
Size: 4073
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI