Restriction map for
pF1KB7218
(Circular) (Six-base) MAPSORT of: pF1KB7218.seq Check: 386 from: 1 to: 3863
pF1KB7218 3863 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 806 901 806
Size: 95 3768
Acc65I G'GTAC_C
Cuts at: 879 879
Size: 3863
AclI AA'CG_TT
Cuts at: 857 3703 857
Size: 2846 1017
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1688 2120 2867 3135 1688
Size: 432 747 268 2416
Fragments arranged by size:
2416 747 432 268
AflIII A'CryG_T
Cuts at: 1215 2319 3296 1215
Size: 1104 977 1782
Fragments arranged by size:
1782 1104 977
AgeI A'CCGG_T
Cuts at: 2251 2251
Size: 3863
AleI CACnn'nnGTG
Cuts at: 594 594
Size: 3863
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 352 384 352
Size: 32 3831
AlwNI CAG_nnn'CTG
Cuts at: 1168 2735 1168
Size: 1567 2296
ApaI G_GGCC'C
Cuts at: 727 727
Size: 3863
ApaLI G'TGCA_C
Cuts at: 2633 2633
Size: 3863
ApoI r'AATT_y
Cuts at: 867 3216 3472 867
Size: 2349 256 1258
Fragments arranged by size:
2349 1258 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3863
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3863
AvaI C'yCGr_G
Cuts at: 883 883
Size: 3863
BamHI G'GATC_C
Cuts at: 836 888 836
Size: 52 3811
BanI G'GyrC_C
Cuts at: 794 879 1293 1541 1576 794
Size: 85 414 248 35 3081
Fragments arranged by size:
3081 414 248 85 35
BanII G_rGCy'C
Cuts at: 727 779 877 1907 727
Size: 52 98 1030 2683
Fragments arranged by size:
2683 1030 98 52
BbeI G_GCGC'C
Cuts at: 798 1297 1545 798
Size: 499 248 3116
Fragments arranged by size:
3116 499 248
BbvCI CC'TCA_GC
Cuts at: 415 502 415
Size: 87 3776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 686 720 686
Size: 34 3829
BciVI GTATCCnnnnn_n'
Cuts at: 1761 2522 3863 1761
Size: 761 1341 1761
Fragments arranged by size:
1761 1341 761
BclI T'GATC_A
Cuts at: 1383 1383
Size: 3863
BglI GCCn_nnn'nGGC
Cuts at: 181 181
Size: 3863
BglII A'GATC_T
Cuts at: 1378 3415 1378
Size: 2037 1826
BlpI GC'TnA_GC
Cuts at: 773 975 773
Size: 202 3661
Bme1580I G_kGCm'C
Cuts at: 727 1488 1581 2637 727
Size: 761 93 1056 1953
Fragments arranged by size:
1953 1056 761 93
BmgBI CAC'GTC
Cuts at: 472 472
Size: 3863
BmrI ACTGGGnnnn_n'
Cuts at: 645 1075 1156 1491 3635 645
Size: 430 81 335 2144 873
Fragments arranged by size:
2144 873 430 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 798 2230 798
Size: 1432 2431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 768 637
Size: 131 3732
Bpu10I CC'TnA_GC
Cuts at: 415 502 415
Size: 87 3776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1918 2410 2708 1039
Size: 879 492 298 2194
Fragments arranged by size:
2194 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3567 3567
Size: 3863
BsaAI yAC'GTr
Cuts at: 1216 1216
Size: 3863
BsaBI GATnn'nnATC
Cuts at: 6 1377 3414 6
Size: 1371 2037 455
Fragments arranged by size:
2037 1371 455
BsaHI Gr'CG_yC
Cuts at: 795 1294 1542 2244 3740 795
Size: 499 248 702 1496 918
Fragments arranged by size:
1496 918 702 499 248
BsaWI w'CCGG_w
Cuts at: 357 1052 1573 2251 2525 2672 3083 357
Size: 695 521 678 274 147 411 1137
Fragments arranged by size:
1137 695 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 352 382 352
Size: 30 3833
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 112 112
Size: 3863
BseYI C'CCAG_C
Cuts at: 114 158 206 548 1288 2046 2623 3119 114
Size: 44 48 342 740 758 577 496 858
Fragments arranged by size:
858 758 740 577 496 342 48 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 508
Size: 3863
BsiEI CG_ry'CG
Cuts at: 104 1405 1451 2659 104
Size: 1301 46 1208 1308
Fragments arranged by size:
1308 1301 1208 46
BsiHKAI G_wGCw'C
Cuts at: 132 335 877 1655 1845 2637 132
Size: 203 542 778 190 792 1358
Fragments arranged by size:
1358 792 778 542 203 190
Bsp1286I G_dGCh'C
Cuts at: 132 335 727 779 877 1488 1581 1655 1845
Size: 203 392 52 98 611 93 74 190
Cuts at: 1845 1907 2637 132
Size: 62 730 1358
Fragments arranged by size:
1358 730 611 392 203 190 98 93
74 62 52
BspEI T'CCGG_A
Cuts at: 357 3083 357
Size: 2726 1137
BspHI T'CATG_A
Cuts at: 3039 3039
Size: 3863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 913 1127 1429 1810 913
Size: 214 302 381 2966
Fragments arranged by size:
2966 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2155 2209 3693 3858 50
Size: 2105 54 1484 165 55
Fragments arranged by size:
2105 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 744 1775 744
Size: 1031 2832
BsrFI r'CCGG_y
Cuts at: 220 1861 2042 2251 220
Size: 1641 181 209 1832
Fragments arranged by size:
1832 1641 209 181
BssSI C'ACGA_G
Cuts at: 2134 2492 2134
Size: 358 3505
BstAPI GCAn_nnn'nTGC
Cuts at: 1240 1240
Size: 3863
BstBI TT'CG_AA
Cuts at: 2224 2224
Size: 3863
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3863
BstYI r'GATC_y
Cuts at: 1 836 888 1355 1378 1713 1959 2971 3057
Size: 835 52 467 23 335 246 1012 86
Cuts at: 3057 3069 3415 1
Size: 12 346 449
Fragments arranged by size:
1012 835 467 449 346 335 246 86
52 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1813 1994 2157 1813
Size: 181 163 3519
Fragments arranged by size:
3519 181 163
BtsI GCAGTG_nn'
Cuts at: 294 538 589 601 1106 294
Size: 244 51 12 505 3051
Fragments arranged by size:
3051 505 244 51 12
DraI TTT'AAA
Cuts at: 862 862
Size: 3863
DrdI GACnn_nn'nnGTC
Cuts at: 1569 2427 1569
Size: 858 3005
EaeI y'GGCC_r
Cuts at: 1448 1622 2013 2040 3385 1448
Size: 174 391 27 1345 1926
Fragments arranged by size:
1926 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1448 1448
Size: 3863
EarI CTCTTCn'nnn_
Cuts at: 232 250 1886 2096 232
Size: 18 1636 210 1999
Fragments arranged by size:
1999 1636 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 310 442 2071 2381 2527 3673 310
Size: 132 1629 310 146 1146 500
Fragments arranged by size:
1629 1146 500 310 146 132
EcoICRI GAG'CTC
Cuts at: 875 875
Size: 3863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 768 1688 2120 2867 3135 637
Size: 131 920 432 747 268 1365
Fragments arranged by size:
1365 920 747 432 268 131
EcoNI CCTnn'n_nnAGG
Cuts at: 685 685
Size: 3863
EcoO109I rG'GnC_Cy
Cuts at: 411 723 724 1002 411
Size: 312 1 278 3272
Fragments arranged by size:
3272 312 278 1
EcoRI G'AATT_C
Cuts at: 867 867
Size: 3863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3863
FseI GG_CCGG'CC
Cuts at: 224 224
Size: 3863
FspI TGC'GCA
Cuts at: 1644 1644
Size: 3863
HaeII r_GCGC'y
Cuts at: 422 577 798 1297 1545 2567 3546 422
Size: 155 221 499 248 1022 979 739
Fragments arranged by size:
1022 979 739 499 248 221 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1705 1735 1737 1767 1705
Size: 30 2 30 3801
Fragments arranged by size:
3801 30 30 2
HincII GTy'rAC
Cuts at: 902 902
Size: 3863
KasI G'GCGC_C
Cuts at: 794 1293 1541 794
Size: 499 248 3116
Fragments arranged by size:
3116 499 248
KpnI G_GTAC'C
Cuts at: 883 883
Size: 3863
MluI A'CGCG_T
Cuts at: 3296 3296
Size: 3863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2534 2718 2534
Size: 184 3679
MscI TGG'CCA
Cuts at: 1624 1624
Size: 3863
MslI CAynn'nnrTG
Cuts at: 434 594 1852 1979 434
Size: 160 1258 127 2318
Fragments arranged by size:
2318 1258 160 127
MspA1I CmG'CkG
Cuts at: 275 289 366 974 1288 1648 2661 2906 275
Size: 14 77 608 314 360 1013 245 1232
Fragments arranged by size:
1232 1013 608 360 314 245 77 14
NaeI GCC'GGC
Cuts at: 222 2044 222
Size: 1822 2041
NarI GG'CG_CC
Cuts at: 795 1294 1542 795
Size: 499 248 3116
Fragments arranged by size:
3116 499 248
NgoMIV G'CCGG_C
Cuts at: 220 2042 220
Size: 1822 2041
NspI r_CATG'y
Cuts at: 531 669 916 1416 2323 531
Size: 138 247 500 907 2071
Fragments arranged by size:
2071 907 500 247 138
PciI A'CATG_T
Cuts at: 2319 2319
Size: 3863
PfoI T'CCnGG_A
Cuts at: 614 614
Size: 3863
PmeI GTTT'AAAC
Cuts at: 862 862
Size: 3863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 352 384 3002 3034 352
Size: 32 2618 32 1181
Fragments arranged by size:
2618 1181 32 32
PpuMI rG'GwC_Cy
Cuts at: 411 411
Size: 3863
PshAI GACnn'nnGTC
Cuts at: 514 514
Size: 3863
PspOMI G'GGCC_C
Cuts at: 723 723
Size: 3863
PstI C_TGCA'G
Cuts at: 596 910 1595 596
Size: 314 685 2864
Fragments arranged by size:
2864 685 314
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3863
PvuII CAG'CTG
Cuts at: 289 366 1288 1648 289
Size: 77 922 360 2504
Fragments arranged by size:
2504 922 360 77
RsrII CG'GwC_CG
Cuts at: 2058 2058
Size: 3863
SacI G_AGCT'C
Cuts at: 877 877
Size: 3863
SalI G'TCGA_C
Cuts at: 900 900
Size: 3863
SapI GCTCTTCn'nnn_
Cuts at: 250 1886 2096 250
Size: 1636 210 2017
Fragments arranged by size:
2017 1636 210
SbfI CC_TGCA'GG
Cuts at: 910 910
Size: 3863
ScaI AGT'ACT
Cuts at: 3380 3380
Size: 3863
SfcI C'TryA_G
Cuts at: 33 592 906 1591 2584 2775 33
Size: 559 314 685 993 191 1121
Fragments arranged by size:
1121 993 685 559 314 191
SfoI GGC'GCC
Cuts at: 796 1295 1543 796
Size: 499 248 3116
Fragments arranged by size:
3116 499 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3863
SmaI CCC'GGG
Cuts at: 885 885
Size: 3863
SmlI C'TyrA_G
Cuts at: 1018 1933 2425 2687 1018
Size: 915 492 262 2194
Fragments arranged by size:
2194 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3863
SphI G_CATG'C
Cuts at: 531 669 916 1416 531
Size: 138 247 500 2978
Fragments arranged by size:
2978 500 247 138
StyI C'CwwG_G
Cuts at: 211 324 569 746 997 1351 211
Size: 113 245 177 251 354 2723
Fragments arranged by size:
2723 354 251 245 177 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1446 1579 2038 2247 1446
Size: 133 459 209 3062
Fragments arranged by size:
3062 459 209 133
TatI w'GTAC_w
Cuts at: 3378 3378
Size: 3863
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 3831
Tth111I GACn'n_nGTC
Cuts at: 1660 1660
Size: 3863
XbaI T'CTAG_A
Cuts at: 894 894
Size: 3863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 715 1358 715
Size: 643 3220
XmaI C'CCGG_G
Cuts at: 883 883
Size: 3863
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AscI AvrII
BaeI BbsI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoRV FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI