Restriction map for
pF1KB7216
(Circular) (Six-base) MAPSORT of: pF1KB7216.seq Check: 5535 from: 1 to: 3560
pF1KB7216 3560 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 598 598
Size: 3560
Acc65I G'GTAC_C
Cuts at: 576 576
Size: 3560
AclI AA'CG_TT
Cuts at: 3400 3400
Size: 3560
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1385 1817 2564 2832 1385
Size: 432 747 268 2113
Fragments arranged by size:
2113 747 432 268
AfeI AGC'GCT
Cuts at: 119 119
Size: 3560
AflIII A'CryG_T
Cuts at: 912 2016 2993 912
Size: 1104 977 1479
Fragments arranged by size:
1479 1104 977
AgeI A'CCGG_T
Cuts at: 1948 1948
Size: 3560
AhdI GACnn_n'nnGTC
Cuts at: 181 181
Size: 3560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 184 184
Size: 3560
AlwNI CAG_nnn'CTG
Cuts at: 865 2432 865
Size: 1567 1993
ApaI G_GGCC'C
Cuts at: 314 314
Size: 3560
ApaLI G'TGCA_C
Cuts at: 2330 2330
Size: 3560
ApoI r'AATT_y
Cuts at: 564 2913 3169 564
Size: 2349 256 955
Fragments arranged by size:
2349 955 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3560
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3560
AvaI C'yCGr_G
Cuts at: 286 580 286
Size: 294 3266
AvrII C'CTAG_G
Cuts at: 219 219
Size: 3560
BamHI G'GATC_C
Cuts at: 585 585
Size: 3560
BanI G'GyrC_C
Cuts at: 223 273 576 990 1238 1273 223
Size: 50 303 414 248 35 2510
Fragments arranged by size:
2510 414 303 248 50 35
BanII G_rGCy'C
Cuts at: 314 371 502 574 1604 314
Size: 57 131 72 1030 2270
Fragments arranged by size:
2270 1030 131 72 57
BbeI G_GCGC'C
Cuts at: 994 1242 994
Size: 248 3312
BciVI GTATCCnnnnn_n'
Cuts at: 1458 2219 3560 1458
Size: 761 1341 1458
Fragments arranged by size:
1458 1341 761
BclI T'GATC_A
Cuts at: 1080 1080
Size: 3560
BglII A'GATC_T
Cuts at: 1075 3112 1075
Size: 2037 1523
BlpI GC'TnA_GC
Cuts at: 672 672
Size: 3560
Bme1580I G_kGCm'C
Cuts at: 314 397 1185 1278 2334 314
Size: 83 788 93 1056 1540
Fragments arranged by size:
1540 1056 788 93 83
BmrI ACTGGGnnnn_n'
Cuts at: 772 853 1188 3332 772
Size: 81 335 2144 1000
Fragments arranged by size:
2144 1000 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1927 1927
Size: 3560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 373 178
Size: 195 3365
Bpu10I CC'TnA_GC
Cuts at: 370 370
Size: 3560
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1615 2107 2405 736
Size: 879 492 298 1891
Fragments arranged by size:
1891 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 272 3264 272
Size: 2992 568
BsaAI yAC'GTr
Cuts at: 913 913
Size: 3560
BsaBI GATnn'nnATC
Cuts at: 6 1074 3111 6
Size: 1068 2037 455
Fragments arranged by size:
2037 1068 455
BsaHI Gr'CG_yC
Cuts at: 991 1239 1941 3437 991
Size: 248 702 1496 1114
Fragments arranged by size:
1496 1114 702 248
BsaWI w'CCGG_w
Cuts at: 466 749 1270 1948 2222 2369 2780 466
Size: 283 521 678 274 147 411 1246
Fragments arranged by size:
1246 678 521 411 283 274 147
BseYI C'CCAG_C
Cuts at: 985 1743 2320 2816 985
Size: 758 577 496 1729
Fragments arranged by size:
1729 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1102 1148 2356 104
Size: 998 46 1208 1308
Fragments arranged by size:
1308 1208 998 46
BsiHKAI G_wGCw'C
Cuts at: 371 574 1352 1542 2334 371
Size: 203 778 190 792 1597
Fragments arranged by size:
1597 792 778 203 190
BsmBI CGTCTCn'nnnn_
Cuts at: 189 189
Size: 3560
Bsp1286I G_dGCh'C
Cuts at: 314 371 397 502 574 1185 1278 1352 1542
Size: 57 26 105 72 611 93 74 190
Cuts at: 1542 1604 2334 314
Size: 62 730 1540
Fragments arranged by size:
1540 730 611 190 105 93 74 72
62 57 26
BspEI T'CCGG_A
Cuts at: 2780 2780
Size: 3560
BspHI T'CATG_A
Cuts at: 2736 2736
Size: 3560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 610 824 1126 1507 610
Size: 214 302 381 2663
Fragments arranged by size:
2663 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1852 1906 3390 3555 50
Size: 1802 54 1484 165 55
Fragments arranged by size:
1802 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1472 1472
Size: 3560
BsrFI r'CCGG_y
Cuts at: 1558 1739 1948 1558
Size: 181 209 3170
Fragments arranged by size:
3170 209 181
BssSI C'ACGA_G
Cuts at: 162 1831 2189 162
Size: 1669 358 1533
Fragments arranged by size:
1669 1533 358
BstAPI GCAn_nnn'nTGC
Cuts at: 937 937
Size: 3560
BstBI TT'CG_AA
Cuts at: 1921 1921
Size: 3560
BstYI r'GATC_y
Cuts at: 1 585 1052 1075 1410 1656 2668 2754 2766
Size: 584 467 23 335 246 1012 86 12
Cuts at: 2766 3112 1
Size: 346 449
Fragments arranged by size:
1012 584 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1510 1691 1854 1510
Size: 181 163 3216
Fragments arranged by size:
3216 181 163
BtsI GCAGTG_nn'
Cuts at: 150 803 150
Size: 653 2907
DraI TTT'AAA
Cuts at: 559 559
Size: 3560
DraIII CAC_nnn'GTG
Cuts at: 403 403
Size: 3560
DrdI GACnn_nn'nnGTC
Cuts at: 1266 2124 1266
Size: 858 2702
EaeI y'GGCC_r
Cuts at: 1145 1319 1710 1737 3082 1145
Size: 174 391 27 1345 1623
Fragments arranged by size:
1623 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1145 1145
Size: 3560
EarI CTCTTCn'nnn_
Cuts at: 1583 1793 1583
Size: 210 3350
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1768 2078 2224 3370 1768
Size: 310 146 1146 1958
Fragments arranged by size:
1958 1146 310 146
EcoICRI GAG'CTC
Cuts at: 369 572 369
Size: 203 3357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 373 1385 1817 2564 2832 178
Size: 195 1012 432 747 268 906
Fragments arranged by size:
1012 906 747 432 268 195
EcoNI CCTnn'n_nnAGG
Cuts at: 232 232
Size: 3560
EcoO109I rG'GnC_Cy
Cuts at: 199 311 699 199
Size: 112 388 3060
Fragments arranged by size:
3060 388 112
EcoRI G'AATT_C
Cuts at: 564 564
Size: 3560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1579 1579
Size: 3560
FspI TGC'GCA
Cuts at: 1341 1341
Size: 3560
HaeII r_GCGC'y
Cuts at: 121 994 1242 2264 3243 121
Size: 873 248 1022 979 438
Fragments arranged by size:
1022 979 873 438 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 167 199 371 403 1402 1432 1434 1464 167
Size: 32 172 32 999 30 2 30 2263
Fragments arranged by size:
2263 999 172 32 32 30 30 2
HincII GTy'rAC
Cuts at: 599 599
Size: 3560
HindIII A'AGCT_T
Cuts at: 247 247
Size: 3560
KasI G'GCGC_C
Cuts at: 990 1238 990
Size: 248 3312
KpnI G_GTAC'C
Cuts at: 580 580
Size: 3560
MluI A'CGCG_T
Cuts at: 2993 2993
Size: 3560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 223 2231 2415 223
Size: 2008 184 1368
Fragments arranged by size:
2008 1368 184
MscI TGG'CCA
Cuts at: 1321 1321
Size: 3560
MslI CAynn'nnrTG
Cuts at: 1549 1676 1549
Size: 127 3433
MspA1I CmG'CkG
Cuts at: 140 477 671 985 1345 2358 2603 140
Size: 337 194 314 360 1013 245 1097
Fragments arranged by size:
1097 1013 360 337 314 245 194
NaeI GCC'GGC
Cuts at: 1741 1741
Size: 3560
NarI GG'CG_CC
Cuts at: 991 1239 991
Size: 248 3312
NgoMIV G'CCGG_C
Cuts at: 1739 1739
Size: 3560
NspI r_CATG'y
Cuts at: 613 1113 2020 613
Size: 500 907 2153
Fragments arranged by size:
2153 907 500
PasI CC'CwG_GG
Cuts at: 228 228
Size: 3560
PciI A'CATG_T
Cuts at: 2016 2016
Size: 3560
PmeI GTTT'AAAC
Cuts at: 559 559
Size: 3560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2699 2731 2699
Size: 32 3528
PpuMI rG'GwC_Cy
Cuts at: 199 199
Size: 3560
PspOMI G'GGCC_C
Cuts at: 310 310
Size: 3560
PstI C_TGCA'G
Cuts at: 145 607 1292 145
Size: 462 685 2413
Fragments arranged by size:
2413 685 462
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3560
PvuII CAG'CTG
Cuts at: 477 985 1345 477
Size: 508 360 2692
Fragments arranged by size:
2692 508 360
RsrII CG'GwC_CG
Cuts at: 1755 1755
Size: 3560
SacI G_AGCT'C
Cuts at: 371 574 371
Size: 203 3357
SalI G'TCGA_C
Cuts at: 597 597
Size: 3560
SanDI GG'GwC_CC
Cuts at: 199 199
Size: 3560
SapI GCTCTTCn'nnn_
Cuts at: 1583 1793 1583
Size: 210 3350
SbfI CC_TGCA'GG
Cuts at: 607 607
Size: 3560
ScaI AGT'ACT
Cuts at: 3077 3077
Size: 3560
SfcI C'TryA_G
Cuts at: 33 141 603 1288 2281 2472 33
Size: 108 462 685 993 191 1121
Fragments arranged by size:
1121 993 685 462 191 108
SfoI GGC'GCC
Cuts at: 992 1240 992
Size: 248 3312
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3560
SmaI CCC'GGG
Cuts at: 582 582
Size: 3560
SmlI C'TyrA_G
Cuts at: 715 1630 2122 2384 715
Size: 915 492 262 1891
Fragments arranged by size:
1891 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3560
SphI G_CATG'C
Cuts at: 613 1113 613
Size: 500 3060
StyI C'CwwG_G
Cuts at: 219 694 1048 219
Size: 475 354 2731
Fragments arranged by size:
2731 475 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 316 338 1143 1276 1735 1944 316
Size: 22 805 133 459 209 1932
Fragments arranged by size:
1932 805 459 209 133 22
TatI w'GTAC_w
Cuts at: 3075 3075
Size: 3560
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3077 3109 3077
Size: 32 3528
Tth111I GACn'n_nGTC
Cuts at: 1357 1357
Size: 3560
XbaI T'CTAG_A
Cuts at: 591 591
Size: 3560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1055 1055
Size: 3560
XmaI C'CCGG_G
Cuts at: 580 580
Size: 3560
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AloI AscI BaeI BbsI
BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI BseRI
BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI