Restriction map for
pF1KB7213
(Circular) (Six-base) MAPSORT of: pF1KB7213.seq Check: 8400 from: 1 to: 3539
pF1KB7213 3539 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 415 415
Size: 3539
AccI GT'mk_AC
Cuts at: 577 577
Size: 3539
Acc65I G'GTAC_C
Cuts at: 555 555
Size: 3539
AclI AA'CG_TT
Cuts at: 3379 3379
Size: 3539
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1364 1796 2543 2811 1364
Size: 432 747 268 2092
Fragments arranged by size:
2092 747 432 268
AflIII A'CryG_T
Cuts at: 891 1995 2972 891
Size: 1104 977 1458
Fragments arranged by size:
1458 1104 977
AgeI A'CCGG_T
Cuts at: 1927 1927
Size: 3539
AlwNI CAG_nnn'CTG
Cuts at: 417 844 2411 417
Size: 427 1567 1545
Fragments arranged by size:
1567 1545 427
ApaI G_GGCC'C
Cuts at: 521 521
Size: 3539
ApaLI G'TGCA_C
Cuts at: 510 2309 510
Size: 1799 1740
ApoI r'AATT_y
Cuts at: 543 2892 3148 543
Size: 2349 256 934
Fragments arranged by size:
2349 934 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3539
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3539
AvaI C'yCGr_G
Cuts at: 559 559
Size: 3539
BamHI G'GATC_C
Cuts at: 564 564
Size: 3539
BanI G'GyrC_C
Cuts at: 469 555 969 1217 1252 469
Size: 86 414 248 35 2756
Fragments arranged by size:
2756 414 248 86 35
BanII G_rGCy'C
Cuts at: 344 521 553 1583 344
Size: 177 32 1030 2300
Fragments arranged by size:
2300 1030 177 32
BbeI G_GCGC'C
Cuts at: 973 1221 973
Size: 248 3291
BciVI GTATCCnnnnn_n'
Cuts at: 1437 2198 3539 1437
Size: 761 1341 1437
Fragments arranged by size:
1437 1341 761
BclI T'GATC_A
Cuts at: 1059 1059
Size: 3539
BglII A'GATC_T
Cuts at: 1054 3091 1054
Size: 2037 1502
BlpI GC'TnA_GC
Cuts at: 651 651
Size: 3539
Bme1580I G_kGCm'C
Cuts at: 472 514 521 1164 1257 2313 472
Size: 42 7 643 93 1056 1698
Fragments arranged by size:
1698 1056 643 93 42 7
BmrI ACTGGGnnnn_n'
Cuts at: 751 832 1167 3311 751
Size: 81 335 2144 979
Fragments arranged by size:
2144 979 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 343
Size: 3539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1594 2086 2384 715
Size: 879 492 298 1870
Fragments arranged by size:
1870 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3243 3243
Size: 3539
BsaAI yAC'GTr
Cuts at: 123 892 123
Size: 769 2770
BsaBI GATnn'nnATC
Cuts at: 6 1053 3090 6
Size: 1047 2037 455
Fragments arranged by size:
2037 1047 455
BsaHI Gr'CG_yC
Cuts at: 318 970 1218 1920 3416 318
Size: 652 248 702 1496 441
Fragments arranged by size:
1496 702 652 441 248
BsaWI w'CCGG_w
Cuts at: 728 1249 1927 2201 2348 2759 728
Size: 521 678 274 147 411 1508
Fragments arranged by size:
1508 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 330 964 1722 2299 2795 330
Size: 634 758 577 496 1074
Fragments arranged by size:
1074 758 634 577 496
BsiEI CG_ry'CG
Cuts at: 104 1081 1127 2335 104
Size: 977 46 1208 1308
Fragments arranged by size:
1308 1208 977 46
BsiHKAI G_wGCw'C
Cuts at: 514 553 1331 1521 2313 514
Size: 39 778 190 792 1740
Fragments arranged by size:
1740 792 778 190 39
Bsp1286I G_dGCh'C
Cuts at: 344 472 514 521 553 1164 1257 1331 1521
Size: 128 42 7 32 611 93 74 190
Cuts at: 1521 1583 2313 344
Size: 62 730 1570
Fragments arranged by size:
1570 730 611 190 128 93 74 62
42 32 7
BspEI T'CCGG_A
Cuts at: 2759 2759
Size: 3539
BspHI T'CATG_A
Cuts at: 2715 2715
Size: 3539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 415 430 589 803 1105 1486 415
Size: 15 159 214 302 381 2468
Fragments arranged by size:
2468 381 302 214 159 15
BsrBI CCG'CTC
Cuts at: 50 1831 1885 3369 3534 50
Size: 1781 54 1484 165 55
Fragments arranged by size:
1781 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1451 1451
Size: 3539
BsrFI r'CCGG_y
Cuts at: 267 394 1537 1718 1927 267
Size: 127 1143 181 209 1879
Fragments arranged by size:
1879 1143 209 181 127
BssSI C'ACGA_G
Cuts at: 1810 2168 1810
Size: 358 3181
BstAPI GCAn_nnn'nTGC
Cuts at: 389 916 389
Size: 527 3012
BstBI TT'CG_AA
Cuts at: 1900 1900
Size: 3539
BstEII G'GTnAC_C
Cuts at: 156 156
Size: 3539
BstYI r'GATC_y
Cuts at: 1 564 1031 1054 1389 1635 2647 2733 2745
Size: 563 467 23 335 246 1012 86 12
Cuts at: 2745 3091 1
Size: 346 449
Fragments arranged by size:
1012 563 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1489 1670 1833 1489
Size: 181 163 3195
Fragments arranged by size:
3195 181 163
BtsI GCAGTG_nn'
Cuts at: 782 782
Size: 3539
DraI TTT'AAA
Cuts at: 538 538
Size: 3539
DrdI GACnn_nn'nnGTC
Cuts at: 207 1245 2103 207
Size: 1038 858 1643
Fragments arranged by size:
1643 1038 858
EaeI y'GGCC_r
Cuts at: 265 1124 1298 1689 1716 3061 265
Size: 859 174 391 27 1345 743
Fragments arranged by size:
1345 859 743 391 174 27
EagI C'GGCC_G
Cuts at: 1124 1124
Size: 3539
EarI CTCTTCn'nnn_
Cuts at: 1562 1772 1562
Size: 210 3329
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 201 1747 2057 2203 3349 201
Size: 1546 310 146 1146 391
Fragments arranged by size:
1546 1146 391 310 146
EcoICRI GAG'CTC
Cuts at: 551 551
Size: 3539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1364 1796 2543 2811 343
Size: 1021 432 747 268 1071
Fragments arranged by size:
1071 1021 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 678 678
Size: 3539
EcoRI G'AATT_C
Cuts at: 543 543
Size: 3539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1558 1558
Size: 3539
FspI TGC'GCA
Cuts at: 1320 1320
Size: 3539
HaeII r_GCGC'y
Cuts at: 973 1221 2243 3222 973
Size: 248 1022 979 1290
Fragments arranged by size:
1290 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1381 1411 1413 1443 1381
Size: 30 2 30 3477
Fragments arranged by size:
3477 30 30 2
HincII GTy'rAC
Cuts at: 578 578
Size: 3539
KasI G'GCGC_C
Cuts at: 969 1217 969
Size: 248 3291
KpnI G_GTAC'C
Cuts at: 559 559
Size: 3539
MluI A'CGCG_T
Cuts at: 2972 2972
Size: 3539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2394 2210
Size: 184 3355
MscI TGG'CCA
Cuts at: 1300 1300
Size: 3539
MslI CAynn'nnrTG
Cuts at: 1528 1655 1528
Size: 127 3412
MspA1I CmG'CkG
Cuts at: 414 650 964 1324 2337 2582 414
Size: 236 314 360 1013 245 1371
Fragments arranged by size:
1371 1013 360 314 245 236
NaeI GCC'GGC
Cuts at: 396 1720 396
Size: 1324 2215
NarI GG'CG_CC
Cuts at: 970 1218 970
Size: 248 3291
NgoMIV G'CCGG_C
Cuts at: 394 1718 394
Size: 1324 2215
NspI r_CATG'y
Cuts at: 592 1092 1999 592
Size: 500 907 2132
Fragments arranged by size:
2132 907 500
PciI A'CATG_T
Cuts at: 1995 1995
Size: 3539
PflMI CCAn_nnn'nTGG
Cuts at: 126 126
Size: 3539
PmeI GTTT'AAAC
Cuts at: 538 538
Size: 3539
PmlI CAC'GTG
Cuts at: 123 123
Size: 3539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2678 2710 2678
Size: 32 3507
PspOMI G'GGCC_C
Cuts at: 517 517
Size: 3539
PstI C_TGCA'G
Cuts at: 221 586 1271 221
Size: 365 685 2489
Fragments arranged by size:
2489 685 365
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3539
PvuII CAG'CTG
Cuts at: 964 1324 964
Size: 360 3179
RsrII CG'GwC_CG
Cuts at: 1734 1734
Size: 3539
SacI G_AGCT'C
Cuts at: 553 553
Size: 3539
SalI G'TCGA_C
Cuts at: 576 576
Size: 3539
SapI GCTCTTCn'nnn_
Cuts at: 1562 1772 1562
Size: 210 3329
SbfI CC_TGCA'GG
Cuts at: 586 586
Size: 3539
ScaI AGT'ACT
Cuts at: 3056 3056
Size: 3539
SexAI A'CCwGG_T
Cuts at: 167 167
Size: 3539
SfcI C'TryA_G
Cuts at: 33 217 582 1267 2260 2451 33
Size: 184 365 685 993 191 1121
Fragments arranged by size:
1121 993 685 365 191 184
SfoI GGC'GCC
Cuts at: 971 1219 971
Size: 248 3291
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3539
SmaI CCC'GGG
Cuts at: 561 561
Size: 3539
SmlI C'TyrA_G
Cuts at: 694 1609 2101 2363 694
Size: 915 492 262 1870
Fragments arranged by size:
1870 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3539
SphI G_CATG'C
Cuts at: 592 1092 592
Size: 500 3039
StyI C'CwwG_G
Cuts at: 673 1027 673
Size: 354 3185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1122 1255 1714 1923 1122
Size: 133 459 209 2738
Fragments arranged by size:
2738 459 209 133
TatI w'GTAC_w
Cuts at: 221 3054 221
Size: 2833 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 3507
Tth111I GACn'n_nGTC
Cuts at: 1336 1336
Size: 3539
XbaI T'CTAG_A
Cuts at: 570 570
Size: 3539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1034 1034
Size: 3539
XmaI C'CCGG_G
Cuts at: 559 559
Size: 3539
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PmlI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI