Restriction map for
pF1KB7211
(Circular) (Six-base) MAPSORT of: pF1KB7211.seq Check: 2671 from: 1 to: 4052
pF1KB7211 4052 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 1090 1090
Size: 4052
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1005 1877 2309 3056 3324 1005
Size: 872 432 747 268 1733
Fragments arranged by size:
1733 872 747 432 268
AflIII A'CryG_T
Cuts at: 826 1404 2508 3485 826
Size: 578 1104 977 1393
Fragments arranged by size:
1393 1104 977 578
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 1357 2924 1357
Size: 1567 2485
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 1056 3405 3661 1056
Size: 2349 256 1447
Fragments arranged by size:
2349 1447 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 1072 1072
Size: 4052
BamHI G'GATC_C
Cuts at: 806 1077 806
Size: 271 3781
BanI G'GyrC_C
Cuts at: 1068 1482 1730 1765 1068
Size: 414 248 35 3355
Fragments arranged by size:
3355 414 248 35
BanII G_rGCy'C
Cuts at: 115 527 1066 2096 115
Size: 412 539 1030 2071
Fragments arranged by size:
2071 1030 539 412
BbeI G_GCGC'C
Cuts at: 1486 1734 1486
Size: 248 3804
BbsI GAAGACnn'nnnn_
Cuts at: 525 630 525
Size: 105 3947
BbvCI CC'TCA_GC
Cuts at: 295 295
Size: 4052
BciVI GTATCCnnnnn_n'
Cuts at: 757 1950 2711 4052 757
Size: 1193 761 1341 757
Fragments arranged by size:
1341 1193 761 757
BclI T'GATC_A
Cuts at: 1572 1572
Size: 4052
BfrBI ATG'CAT
Cuts at: 398 398
Size: 4052
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 1677 1770 2826 1677
Size: 93 1056 2903
Fragments arranged by size:
2903 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
Bpu10I CC'TnA_GC
Cuts at: 295 295
Size: 4052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1228 2107 2599 2897 646
Size: 582 879 492 298 1801
Fragments arranged by size:
1801 879 582 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3756 3756
Size: 4052
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 1483 1731 2433 3929 1483
Size: 248 702 1496 1606
Fragments arranged by size:
1606 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 225 1477 2235 2812 3308 225
Size: 1252 758 577 496 969
Fragments arranged by size:
1252 969 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 527 1066 1844 2034 2826 527
Size: 539 778 190 792 1753
Fragments arranged by size:
1753 792 778 539 190
BsmI GAATG_Cn'
Cuts at: 400 400
Size: 4052
Bsp1286I G_dGCh'C
Cuts at: 115 527 1066 1677 1770 1844 2034 2096 2826
Size: 412 539 611 93 74 190 62 730
Cuts at: 2826 115
Size: 1341
Fragments arranged by size:
1341 730 611 539 412 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 512 3228 512
Size: 2716 1336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 1102 1316 1618 1999 242
Size: 860 214 302 381 2295
Fragments arranged by size:
2295 860 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1964 1964
Size: 4052
BsrFI r'CCGG_y
Cuts at: 2050 2231 2440 2050
Size: 181 209 3662
Fragments arranged by size:
3662 209 181
BsrGI T'GTAC_A
Cuts at: 938 938
Size: 4052
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 225 225
Size: 4052
BstYI r'GATC_y
Cuts at: 1 806 1077 1544 1567 1902 2148 3160 3246
Size: 805 271 467 23 335 246 1012 86
Cuts at: 3246 3258 3604 1
Size: 12 346 449
Fragments arranged by size:
1012 805 467 449 346 335 271 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 784 982 784
Size: 198 3854
BtgI C'CryG_G
Cuts at: 218 218
Size: 4052
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 1295 1295
Size: 4052
ClaI AT'CG_AT
Cuts at: 379 379
Size: 4052
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 603 919 1758 2616 603
Size: 316 839 858 2039
Fragments arranged by size:
2039 858 839 316
EaeI y'GGCC_r
Cuts at: 166 876 1637 1811 2202 2229 3574 166
Size: 710 761 174 391 27 1345 644
Fragments arranged by size:
1345 761 710 644 391 174 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 515 1024 2075 2285 515
Size: 509 1051 210 2282
Fragments arranged by size:
2282 1051 509 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 525 1064 525
Size: 539 3513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1005 1877 2309 3056 3324 1005
Size: 872 432 747 268 1733
Fragments arranged by size:
1733 872 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 388 388
Size: 4052
EcoO109I rG'GnC_Cy
Cuts at: 789 1005 1191 789
Size: 216 186 3650
Fragments arranged by size:
3650 216 186
EcoRI G'AATT_C
Cuts at: 1056 1056
Size: 4052
EcoRV GAT'ATC
Cuts at: 681 681
Size: 4052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1024 2071 1024
Size: 1047 3005
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 1486 1734 2756 3735 1486
Size: 248 1022 979 1803
Fragments arranged by size:
1803 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1894 1924 1926 1956 1894
Size: 30 2 30 3990
Fragments arranged by size:
3990 30 30 2
HincII GTy'rAC
Cuts at: 1091 1091
Size: 4052
KasI G'GCGC_C
Cuts at: 1482 1730 1482
Size: 248 3804
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2723 2907 2723
Size: 184 3868
MscI TGG'CCA
Cuts at: 878 1813 878
Size: 935 3117
MslI CAynn'nnrTG
Cuts at: 280 741 955 2041 2168 280
Size: 461 214 1086 127 2164
Fragments arranged by size:
2164 1086 461 214 127
MspA1I CmG'CkG
Cuts at: 258 1163 1477 1837 2850 3095 258
Size: 905 314 360 1013 245 1215
Fragments arranged by size:
1215 1013 905 360 314 245
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 1483 1731 1483
Size: 248 3804
NdeI CA'TA_TG
Cuts at: 465 484 759 465
Size: 19 275 3758
Fragments arranged by size:
3758 275 19
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NsiI A_TGCA'T
Cuts at: 400 400
Size: 4052
NspI r_CATG'y
Cuts at: 210 363 830 1105 1605 2512 210
Size: 153 467 275 500 907 1750
Fragments arranged by size:
1750 907 500 467 275 153
PciI A'CATG_T
Cuts at: 826 2508 826
Size: 1682 2370
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PpuMI rG'GwC_Cy
Cuts at: 789 789
Size: 4052
PstI C_TGCA'G
Cuts at: 1099 1784 1099
Size: 685 3367
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 258 1477 1837 258
Size: 1219 360 2473
Fragments arranged by size:
2473 1219 360
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 527 1066 527
Size: 539 3513
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SanDI GG'GwC_CC
Cuts at: 789 789
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 515 2075 2285 515
Size: 1560 210 2282
Fragments arranged by size:
2282 1560 210
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SfcI C'TryA_G
Cuts at: 33 975 1095 1780 2773 2964 33
Size: 942 120 685 993 191 1121
Fragments arranged by size:
1121 993 942 685 191 120
SfoI GGC'GCC
Cuts at: 1484 1732 1484
Size: 248 3804
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 661 1207 2122 2614 2876 661
Size: 546 915 492 262 1837
Fragments arranged by size:
1837 915 546 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
SspI AAT'ATT
Cuts at: 872 872
Size: 4052
StyI C'CwwG_G
Cuts at: 1008 1186 1540 1008
Size: 178 354 3520
Fragments arranged by size:
3520 354 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 485 969 1635 1768 2227 2436 485
Size: 484 666 133 459 209 2101
Fragments arranged by size:
2101 666 484 459 209 133
TatI w'GTAC_w
Cuts at: 284 371 938 3567 284
Size: 87 567 2629 769
Fragments arranged by size:
2629 769 567 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1547 1547
Size: 4052
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
XmnI GAAnn'nnTTC
Cuts at: 1018 1018
Size: 4052
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BglI BmgBI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmBI BssHII BstEII
BstZ17I CspCI DraIII FseI FspAI HindIII HpaI MfeI
NcoI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI