Restriction map for
pF1KB7206
(Circular) (Six-base) MAPSORT of: pF1KB7206.seq Check: 4975 from: 1 to: 4838
pF1KB7206 4838 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AatII G_ACGT'C
Cuts at: 968 968
Size: 4838
AccI GT'mk_AC
Cuts at: 731 1876 731
Size: 1145 3693
Acc65I G'GTAC_C
Cuts at: 1854 1854
Size: 4838
AclI AA'CG_TT
Cuts at: 4678 4678
Size: 4838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2663 3095 3842 4110 2663
Size: 432 747 268 3391
Fragments arranged by size:
3391 747 432 268
AfeI AGC'GCT
Cuts at: 1478 1478
Size: 4838
AflIII A'CryG_T
Cuts at: 1802 2190 3294 4271 1802
Size: 388 1104 977 2369
Fragments arranged by size:
2369 1104 977 388
AgeI A'CCGG_T
Cuts at: 3226 3226
Size: 4838
AleI CACnn'nnGTG
Cuts at: 751 751
Size: 4838
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 140 140
Size: 4838
AlwNI CAG_nnn'CTG
Cuts at: 418 1440 2143 3710 418
Size: 1022 703 1567 1546
Fragments arranged by size:
1567 1546 1022 703
ApaI G_GGCC'C
Cuts at: 493 493
Size: 4838
ApaLI G'TGCA_C
Cuts at: 3608 3608
Size: 4838
ApoI r'AATT_y
Cuts at: 1111 1567 1842 4191 4447 1111
Size: 456 275 2349 256 1502
Fragments arranged by size:
2349 1502 456 275 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4838
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
AvaI C'yCGr_G
Cuts at: 397 695 1405 1858 397
Size: 298 710 453 3377
Fragments arranged by size:
3377 710 453 298
AvrII C'CTAG_G
Cuts at: 885 885
Size: 4838
BamHI G'GATC_C
Cuts at: 1430 1863 1430
Size: 433 4405
BanI G'GyrC_C
Cuts at: 150 1854 2268 2516 2551 150
Size: 1704 414 248 35 2437
Fragments arranged by size:
2437 1704 414 248 35
BanII G_rGCy'C
Cuts at: 493 1276 1377 1852 2882 493
Size: 783 101 475 1030 2449
Fragments arranged by size:
2449 1030 783 475 101
BbeI G_GCGC'C
Cuts at: 2272 2520 2272
Size: 248 4590
BbsI GAAGACnn'nnnn_
Cuts at: 1633 1633
Size: 4838
BbvCI CC'TCA_GC
Cuts at: 643 643
Size: 4838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 259 293 90
Size: 34 135 34 4635
Fragments arranged by size:
4635 135 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2736 3497 4838 2736
Size: 761 1341 2736
Fragments arranged by size:
2736 1341 761
BclI T'GATC_A
Cuts at: 2358 2358
Size: 4838
BfrBI ATG'CAT
Cuts at: 376 376
Size: 4838
BglI GCCn_nnn'nGGC
Cuts at: 392 481 554 563 392
Size: 89 73 9 4667
Fragments arranged by size:
4667 89 73 9
BglII A'GATC_T
Cuts at: 2353 4390 2353
Size: 2037 2801
BlpI GC'TnA_GC
Cuts at: 281 1825 1950 281
Size: 1544 125 3169
Fragments arranged by size:
3169 1544 125
Bme1580I G_kGCm'C
Cuts at: 155 493 511 2463 2556 3612 155
Size: 338 18 1952 93 1056 1381
Fragments arranged by size:
1952 1381 1056 338 93 18
BmgBI CAC'GTC
Cuts at: 1557 1803 1557
Size: 246 4592
BmrI ACTGGGnnnn_n'
Cuts at: 202 488 1378 2050 2131 2466 4610 202
Size: 286 890 672 81 335 2144 430
Fragments arranged by size:
2144 890 672 430 335 286 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 592 937 1177 1618 3205 592
Size: 345 240 441 1587 2225
Fragments arranged by size:
2225 1587 441 345 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 488 533 1071 1138 1172 1552 1642 473
Size: 15 45 538 67 34 380 90 3669
Fragments arranged by size:
3669 538 380 90 67 45 34 15
Bpu10I CC'TnA_GC
Cuts at: 643 736 643
Size: 93 4745
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 2014 2893 3385 3683 337
Size: 1677 879 492 298 1492
Fragments arranged by size:
1677 1492 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 256 580 1518 4542 256
Size: 324 938 3024 552
Fragments arranged by size:
3024 938 552 324
BsaAI yAC'GTr
Cuts at: 2191 2191
Size: 4838
BsaBI GATnn'nnATC
Cuts at: 6 2352 4389 6
Size: 2346 2037 455
Fragments arranged by size:
2346 2037 455
BsaHI Gr'CG_yC
Cuts at: 965 1751 2269 2517 3219 4715 965
Size: 786 518 248 702 1496 1088
Fragments arranged by size:
1496 1088 786 702 518 248
BsaWI w'CCGG_w
Cuts at: 2027 2548 3226 3500 3647 4058 2027
Size: 521 678 274 147 411 2807
Fragments arranged by size:
2807 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 596 626 1069 1099 596
Size: 30 443 30 4335
Fragments arranged by size:
4335 443 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 617 1169 617
Size: 552 4286
BseYI C'CCAG_C
Cuts at: 285 504 1005 1026 1244 1517 1740 2263 3021
Size: 219 501 21 218 273 223 523 758
Cuts at: 3021 3598 4094 285
Size: 577 496 1029
Fragments arranged by size:
1029 758 577 523 501 496 273 223
219 218 21
BsiEI CG_ry'CG
Cuts at: 104 2380 2426 3634 104
Size: 2276 46 1208 1308
Fragments arranged by size:
2276 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 626 1852 2630 2820 3612 626
Size: 1226 778 190 792 1852
Fragments arranged by size:
1852 1226 792 778 190
BsmI GAATG_Cn'
Cuts at: 378 1714 378
Size: 1336 3502
Bsp1286I G_dGCh'C
Cuts at: 155 493 511 626 1276 1377 1852 2463 2556
Size: 338 18 115 650 101 475 611 93
Cuts at: 2556 2630 2820 2882 3612 155
Size: 74 190 62 730 1381
Fragments arranged by size:
1381 730 650 611 475 338 190 115
101 93 74 62 18
BspEI T'CCGG_A
Cuts at: 4058 4058
Size: 4838
BspHI T'CATG_A
Cuts at: 1604 4014 1604
Size: 2410 2428
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1888 2102 2404 2785 1888
Size: 214 302 381 3941
Fragments arranged by size:
3941 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3130 3184 4668 4833 50
Size: 3080 54 1484 165 55
Fragments arranged by size:
3080 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1733 1794 2750 1733
Size: 61 956 3821
Fragments arranged by size:
3821 956 61
BsrFI r'CCGG_y
Cuts at: 434 2836 3017 3226 434
Size: 2402 181 209 2046
Fragments arranged by size:
2402 2046 209 181
BsrGI T'GTAC_A
Cuts at: 743 743
Size: 4838
BssSI C'ACGA_G
Cuts at: 931 1033 3109 3467 931
Size: 102 2076 358 2302
Fragments arranged by size:
2302 2076 358 102
BstAPI GCAn_nnn'nTGC
Cuts at: 2215 2215
Size: 4838
BstBI TT'CG_AA
Cuts at: 3199 3199
Size: 4838
BstXI CCAn_nnnn'nTGG
Cuts at: 933 1181 1269 1530 933
Size: 248 88 261 4241
Fragments arranged by size:
4241 261 248 88
BstYI r'GATC_y
Cuts at: 1 311 1430 1863 2330 2353 2688 2934 3946
Size: 310 1119 433 467 23 335 246 1012
Cuts at: 3946 4032 4044 4390 1
Size: 86 12 346 449
Fragments arranged by size:
1119 1012 467 449 433 346 335 310
246 86 23 12
BtgI C'CryG_G
Cuts at: 833 1223 833
Size: 390 4448
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 777 885 2788 2969 3132 777
Size: 108 1903 181 163 2483
Fragments arranged by size:
2483 1903 181 163 108
BtsI GCAGTG_nn'
Cuts at: 275 369 1496 2081 275
Size: 94 1127 585 3032
Fragments arranged by size:
3032 1127 585 94
DraI TTT'AAA
Cuts at: 1837 1837
Size: 4838
DrdI GACnn_nn'nnGTC
Cuts at: 190 2544 3402 190
Size: 2354 858 1626
Fragments arranged by size:
2354 1626 858
EaeI y'GGCC_r
Cuts at: 755 959 1183 1250 1493 2423 2597 2988 3015
Size: 204 224 67 243 930 174 391 27
Cuts at: 3015 4360 755
Size: 1345 1233
Fragments arranged by size:
1345 1233 930 391 243 224 204 174
67 27
EagI C'GGCC_G
Cuts at: 2423 2423
Size: 4838
EarI CTCTTCn'nnn_
Cuts at: 763 1334 2861 3071 763
Size: 571 1527 210 2530
Fragments arranged by size:
2530 1527 571 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 581 3046 3356 3502 4648 581
Size: 2465 310 146 1146 771
Fragments arranged by size:
2465 1146 771 310 146
EcoICRI GAG'CTC
Cuts at: 1850 1850
Size: 4838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 488 533 1071 1138 1172 1552 1642 2663
Size: 15 45 538 67 34 380 90 1021
Cuts at: 2663 3095 3842 4110 473
Size: 432 747 268 1201
Fragments arranged by size:
1201 1021 747 538 432 380 268 90
67 45 34 15
EcoNI CCTnn'n_nnAGG
Cuts at: 145 1681 145
Size: 1536 3302
EcoO109I rG'GnC_Cy
Cuts at: 199 339 393 1812 1977 199
Size: 140 54 1419 165 3060
Fragments arranged by size:
3060 1419 165 140 54
EcoRI G'AATT_C
Cuts at: 1111 1567 1842 1111
Size: 456 275 4107
Fragments arranged by size:
4107 456 275
EcoRV GAT'ATC
Cuts at: 1320 1320
Size: 4838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 4838
FspI TGC'GCA
Cuts at: 2619 2619
Size: 4838
HaeII r_GCGC'y
Cuts at: 1480 2272 2520 3542 4521 1480
Size: 792 248 1022 979 1797
Fragments arranged by size:
1797 1022 979 792 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 596 628 678 710 1148 1180 1364 1396 2680
Size: 32 50 32 438 32 184 32 1284
Cuts at: 2680 2710 2712 2742 596
Size: 30 2 30 2692
Fragments arranged by size:
2692 1284 438 184 50 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1877 1877
Size: 4838
KasI G'GCGC_C
Cuts at: 2268 2516 2268
Size: 248 4590
KpnI G_GTAC'C
Cuts at: 1858 1858
Size: 4838
MluI A'CGCG_T
Cuts at: 4271 4271
Size: 4838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1787 3509 3693 1787
Size: 1722 184 2932
Fragments arranged by size:
2932 1722 184
MscI TGG'CCA
Cuts at: 757 1185 1252 1495 2599 757
Size: 428 67 243 1104 2996
Fragments arranged by size:
2996 1104 428 243 67
MslI CAynn'nnrTG
Cuts at: 751 1179 1555 1684 1801 2827 2954 751
Size: 428 376 129 117 1026 127 2635
Fragments arranged by size:
2635 1026 428 376 129 127 117
MspA1I CmG'CkG
Cuts at: 285 529 1026 1189 1248 1949 2263 2623 3636
Size: 244 497 163 59 701 314 360 1013
Cuts at: 3636 3881 285
Size: 245 1242
Fragments arranged by size:
1242 1013 701 497 360 314 245 244
163 59
NaeI GCC'GGC
Cuts at: 3019 3019
Size: 4838
NarI GG'CG_CC
Cuts at: 2269 2517 2269
Size: 248 4590
NcoI C'CATG_G
Cuts at: 833 1223 833
Size: 390 4448
NdeI CA'TA_TG
Cuts at: 378 378
Size: 4838
NgoMIV G'CCGG_C
Cuts at: 3017 3017
Size: 4838
NsiI A_TGCA'T
Cuts at: 378 378
Size: 4838
NspI r_CATG'y
Cuts at: 522 1306 1335 1891 2391 3298 522
Size: 784 29 556 500 907 2062
Fragments arranged by size:
2062 907 784 556 500 29
PciI A'CATG_T
Cuts at: 3294 3294
Size: 4838
PfoI T'CCnGG_A
Cuts at: 785 1115 785
Size: 330 4508
PmeI GTTT'AAAC
Cuts at: 1837 1837
Size: 4838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 3977
Size: 32 4806
PpuMI rG'GwC_Cy
Cuts at: 199 339 1812 199
Size: 140 1473 3225
Fragments arranged by size:
3225 1473 140
PspOMI G'GGCC_C
Cuts at: 489 489
Size: 4838
PstI C_TGCA'G
Cuts at: 116 449 1295 1466 1885 2570 116
Size: 333 846 171 419 685 2384
Fragments arranged by size:
2384 846 685 419 333 171
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4838
PvuII CAG'CTG
Cuts at: 285 529 1026 1189 1248 2263 2623 285
Size: 244 497 163 59 1015 360 2500
Fragments arranged by size:
2500 1015 497 360 244 163 59
RsrII CG'GwC_CG
Cuts at: 3033 3033
Size: 4838
SacI G_AGCT'C
Cuts at: 1852 1852
Size: 4838
SalI G'TCGA_C
Cuts at: 1875 1875
Size: 4838
SapI GCTCTTCn'nnn_
Cuts at: 1334 2861 3071 1334
Size: 1527 210 3101
Fragments arranged by size:
3101 1527 210
SbfI CC_TGCA'GG
Cuts at: 1885 1885
Size: 4838
ScaI AGT'ACT
Cuts at: 4355 4355
Size: 4838
SfcI C'TryA_G
Cuts at: 33 112 445 1291 1462 1881 2566 3559 3750
Size: 79 333 846 171 419 685 993 191
Cuts at: 3750 33
Size: 1121
Fragments arranged by size:
1121 993 846 685 419 333 191 171
79
SfiI GGCCn_nnn'nGGCC
Cuts at: 392 554 392
Size: 162 4676
SfoI GGC'GCC
Cuts at: 2270 2518 2270
Size: 248 4590
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
SgrAI Cr'CCGG_yG
Cuts at: 434 434
Size: 4838
SmaI CCC'GGG
Cuts at: 1860 1860
Size: 4838
SmlI C'TyrA_G
Cuts at: 316 1993 2908 3400 3662 316
Size: 1677 915 492 262 1492
Fragments arranged by size:
1677 1492 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4838
SphI G_CATG'C
Cuts at: 522 1306 1891 2391 522
Size: 784 585 500 2969
Fragments arranged by size:
2969 784 585 500
StyI C'CwwG_G
Cuts at: 155 549 579 818 833 885 1223 1385 1972
Size: 394 30 239 15 52 338 162 587
Cuts at: 1972 2326 155
Size: 354 2667
Fragments arranged by size:
2667 587 394 354 338 239 162 52
30 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 148 715 1216 2421 2554 3013 3222 148
Size: 567 501 1205 133 459 209 1764
Fragments arranged by size:
1764 1205 567 501 459 209 133
TatI w'GTAC_w
Cuts at: 743 1076 4353 743
Size: 333 3277 1228
Fragments arranged by size:
3277 1228 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 834 866 4355 4387 834
Size: 32 3489 32 1285
Fragments arranged by size:
3489 1285 32 32
Tth111I GACn'n_nGTC
Cuts at: 2635 2635
Size: 4838
XbaI T'CTAG_A
Cuts at: 1308 1869 1308
Size: 561 4277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 483 996 1245 1365 2333 483
Size: 513 249 120 968 2988
Fragments arranged by size:
2988 968 513 249 120
XmaI C'CCGG_G
Cuts at: 1858 1858
Size: 4838
XmnI GAAnn'nnTTC
Cuts at: 1343 1343
Size: 4838
ZraI GAC'GTC
Cuts at: 966 966
Size: 4838
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AloI AscI BaeI BmtI BsgI
BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII FseI FspAI HindIII HpaI MfeI NheI NotI
NruI PacI PasI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SnaBI SrfI SspI StuI
SwaI XhoI