Restriction map for
pF1KB7205
(Circular) (Six-base) MAPSORT of: pF1KB7205.seq Check: 8286 from: 1 to: 4391
pF1KB7205 4391 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1145 1145
Size: 4391
AccI GT'mk_AC
Cuts at: 1429 1429
Size: 4391
Acc65I G'GTAC_C
Cuts at: 1407 1407
Size: 4391
AclI AA'CG_TT
Cuts at: 4231 4231
Size: 4391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2216 2648 3395 3663 2216
Size: 432 747 268 2944
Fragments arranged by size:
2944 747 432 268
AflIII A'CryG_T
Cuts at: 1743 2847 3824 1743
Size: 1104 977 2310
Fragments arranged by size:
2310 1104 977
AgeI A'CCGG_T
Cuts at: 2779 2779
Size: 4391
AlwNI CAG_nnn'CTG
Cuts at: 342 933 1696 3263 342
Size: 591 763 1567 1470
Fragments arranged by size:
1567 1470 763 591
ApaI G_GGCC'C
Cuts at: 186 243 850 1002 1381 186
Size: 57 607 152 379 3196
Fragments arranged by size:
3196 607 379 152 57
ApaLI G'TGCA_C
Cuts at: 529 3161 529
Size: 2632 1759
ApoI r'AATT_y
Cuts at: 1395 3744 4000 1395
Size: 2349 256 1786
Fragments arranged by size:
2349 1786 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4391
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
AvaI C'yCGr_G
Cuts at: 179 481 502 854 920 1159 1411 179
Size: 302 21 352 66 239 252 3159
Fragments arranged by size:
3159 352 302 252 239 66 21
BamHI G'GATC_C
Cuts at: 887 1416 887
Size: 529 3862
BanI G'GyrC_C
Cuts at: 537 1163 1281 1358 1407 1821 2069 2104 537
Size: 626 118 77 49 414 248 35 2824
Fragments arranged by size:
2824 626 414 248 118 77 49 35
BanII G_rGCy'C
Cuts at: 186 243 502 840 850 1002 1381 1405 2435
Size: 57 259 338 10 152 379 24 1030
Cuts at: 2435 186
Size: 2142
Fragments arranged by size:
2142 1030 379 338 259 152 57 24
10
BbeI G_GCGC'C
Cuts at: 1825 2073 1825
Size: 248 4143
BbsI GAAGACnn'nnnn_
Cuts at: 757 757
Size: 4391
BbvCI CC'TCA_GC
Cuts at: 548 562 548
Size: 14 4377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 510 544 510
Size: 34 4357
BciVI GTATCCnnnnn_n'
Cuts at: 2289 3050 4391 2289
Size: 761 1341 2289
Fragments arranged by size:
2289 1341 761
BclI T'GATC_A
Cuts at: 1911 1911
Size: 4391
BglI GCCn_nnn'nGGC
Cuts at: 247 619 247
Size: 372 4019
BglII A'GATC_T
Cuts at: 815 1906 3943 815
Size: 1091 2037 1263
Fragments arranged by size:
2037 1263 1091
BlpI GC'TnA_GC
Cuts at: 1503 1503
Size: 4391
Bme1580I G_kGCm'C
Cuts at: 186 243 456 533 542 850 1002 1168 1286
Size: 57 213 77 9 308 152 166 118
Cuts at: 1286 1381 2016 2109 3165 186
Size: 95 635 93 1056 1412
Fragments arranged by size:
1412 1056 635 308 213 166 152 118
95 93 77 57 9
BmrI ACTGGGnnnn_n'
Cuts at: 1603 1684 2019 4163 1603
Size: 81 335 2144 1831
Fragments arranged by size:
2144 1831 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 300 2758 300
Size: 2458 1933
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 466 476 534 664 694 976 1259 1368
Size: 102 10 58 130 30 282 283 109
Cuts at: 1368 364
Size: 3387
Fragments arranged by size:
3387 283 282 130 109 102 58 30
10
Bpu10I CC'TnA_GC
Cuts at: 548 562 548
Size: 14 4377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 1567 2446 2938 3236 931
Size: 636 879 492 298 2086
Fragments arranged by size:
2086 879 636 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1239 1344 4095 1239
Size: 105 2751 1535
Fragments arranged by size:
2751 1535 105
BsaAI yAC'GTr
Cuts at: 843 1744 843
Size: 901 3490
BsaBI GATnn'nnATC
Cuts at: 6 1905 3942 6
Size: 1899 2037 455
Fragments arranged by size:
2037 1899 455
BsaHI Gr'CG_yC
Cuts at: 1822 2070 2772 4268 1822
Size: 248 702 1496 1945
Fragments arranged by size:
1945 1496 702 248
BsaWI w'CCGG_w
Cuts at: 390 1058 1580 2101 2779 3053 3200 3611 390
Size: 668 522 521 678 274 147 411 1170
Fragments arranged by size:
1170 678 668 522 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 397 427 827 857 1082 1112 397
Size: 30 400 30 225 30 3676
Fragments arranged by size:
3676 400 225 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 378 421 378
Size: 43 4348
BseYI C'CCAG_C
Cuts at: 348 872 1269 1332 1816 2574 3151 3647 348
Size: 524 397 63 484 758 577 496 1092
Fragments arranged by size:
1092 758 577 524 496 484 397 63
BsiEI CG_ry'CG
Cuts at: 104 237 1933 1979 3187 104
Size: 133 1696 46 1208 1308
Fragments arranged by size:
1696 1308 1208 133 46
BsiHKAI G_wGCw'C
Cuts at: 533 1405 2183 2373 3165 533
Size: 872 778 190 792 1759
Fragments arranged by size:
1759 872 792 778 190
BsmI GAATG_Cn'
Cuts at: 903 903
Size: 4391
BsmBI CGTCTCn'nnnn_
Cuts at: 1110 1110
Size: 4391
Bsp1286I G_dGCh'C
Cuts at: 186 243 456 502 533 542 840 850 1002
Size: 57 213 46 31 9 298 10 152
Cuts at: 1002 1168 1286 1381 1405 2016 2109 2183 2373
Size: 166 118 95 24 611 93 74 190
Cuts at: 2373 2435 3165 186
Size: 62 730 1412
Fragments arranged by size:
1412 730 611 298 213 190 166 152
118 95 93 74 62 57 46 31
24 10 9
BspEI T'CCGG_A
Cuts at: 390 1058 3611 390
Size: 668 2553 1170
Fragments arranged by size:
2553 1170 668
BspHI T'CATG_A
Cuts at: 969 3567 969
Size: 2598 1793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1145 1441 1655 1957 2338 1145
Size: 296 214 302 381 3198
Fragments arranged by size:
3198 381 302 296 214
BsrBI CCG'CTC
Cuts at: 50 2683 2737 4221 4386 50
Size: 2633 54 1484 165 55
Fragments arranged by size:
2633 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1128 2303 1128
Size: 1175 3216
BsrFI r'CCGG_y
Cuts at: 112 398 2389 2570 2779 112
Size: 286 1991 181 209 1724
Fragments arranged by size:
1991 1724 286 209 181
BsrGI T'GTAC_A
Cuts at: 413 413
Size: 4391
BssSI C'ACGA_G
Cuts at: 299 595 2662 3020 299
Size: 296 2067 358 1670
Fragments arranged by size:
2067 1670 358 296
BstAPI GCAn_nnn'nTGC
Cuts at: 537 1768 537
Size: 1231 3160
BstBI TT'CG_AA
Cuts at: 2752 2752
Size: 4391
BstXI CCAn_nnnn'nTGG
Cuts at: 139 139
Size: 4391
BstYI r'GATC_y
Cuts at: 1 815 887 1416 1883 1906 2241 2487 3499
Size: 814 72 529 467 23 335 246 1012
Cuts at: 3499 3585 3597 3943 1
Size: 86 12 346 449
Fragments arranged by size:
1012 814 529 467 449 346 335 246
86 72 23 12
Bsu36I CC'TnA_GG
Cuts at: 361 361
Size: 4391
BtgI C'CryG_G
Cuts at: 107 228 260 320 542 759 1227 1242 1382
Size: 121 32 60 222 217 468 15 140
Cuts at: 1382 107
Size: 3116
Fragments arranged by size:
3116 468 222 217 140 121 60 32
15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2341 2522 2685 2341
Size: 181 163 4047
Fragments arranged by size:
4047 181 163
BtsI GCAGTG_nn'
Cuts at: 709 1634 709
Size: 925 3466
DraI TTT'AAA
Cuts at: 1390 1390
Size: 4391
DraIII CAC_nnn'GTG
Cuts at: 293 537 293
Size: 244 4147
DrdI GACnn_nn'nnGTC
Cuts at: 2097 2955 2097
Size: 858 3533
EaeI y'GGCC_r
Cuts at: 110 295 1292 1976 2150 2541 2568 3913 110
Size: 185 997 684 174 391 27 1345 588
Fragments arranged by size:
1345 997 684 588 391 185 174 27
EagI C'GGCC_G
Cuts at: 1976 1976
Size: 4391
EarI CTCTTCn'nnn_
Cuts at: 1117 2414 2624 1117
Size: 1297 210 2884
Fragments arranged by size:
2884 1297 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 214 322 1119 2599 2909 3055 4201 214
Size: 108 797 1480 310 146 1146 404
Fragments arranged by size:
1480 1146 797 404 310 146 108
EcoICRI GAG'CTC
Cuts at: 1403 1403
Size: 4391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 466 476 534 664 694 976 1259 1368
Size: 102 10 58 130 30 282 283 109
Cuts at: 1368 2216 2648 3395 3663 364
Size: 848 432 747 268 1092
Fragments arranged by size:
1092 848 747 432 283 282 268 130
109 102 58 30 10
EcoO109I rG'GnC_Cy
Cuts at: 183 196 240 639 998 999 1377 1378 1530
Size: 13 44 399 359 1 378 1 152
Cuts at: 1530 183
Size: 3044
Fragments arranged by size:
3044 399 378 359 152 44 13 1
1
EcoRI G'AATT_C
Cuts at: 1395 1395
Size: 4391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4391
FseI GG_CCGG'CC
Cuts at: 116 116
Size: 4391
FspI TGC'GCA
Cuts at: 577 715 2172 577
Size: 138 1457 2796
Fragments arranged by size:
2796 1457 138
HaeII r_GCGC'y
Cuts at: 933 1825 2073 3095 4074 933
Size: 892 248 1022 979 1250
Fragments arranged by size:
1250 1022 979 892 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1082 1114 2233 2263 2265 2295 1082
Size: 32 1119 30 2 30 3178
Fragments arranged by size:
3178 1119 32 30 30 2
HincII GTy'rAC
Cuts at: 1430 1430
Size: 4391
KasI G'GCGC_C
Cuts at: 1821 2069 1821
Size: 248 4143
KpnI G_GTAC'C
Cuts at: 1411 1411
Size: 4391
MluI A'CGCG_T
Cuts at: 3824 3824
Size: 4391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 681 3062 3246 681
Size: 2381 184 1826
Fragments arranged by size:
2381 1826 184
MscI TGG'CCA
Cuts at: 297 2152 297
Size: 1855 2536
MslI CAynn'nnrTG
Cuts at: 742 2380 2507 742
Size: 1638 127 2626
Fragments arranged by size:
2626 1638 127
MspA1I CmG'CkG
Cuts at: 246 315 342 512 581 876 1200 1502 1816
Size: 69 27 170 69 295 324 302 314
Cuts at: 1816 2176 3189 3434 246
Size: 360 1013 245 1203
Fragments arranged by size:
1203 1013 360 324 314 302 295 245
170 69 69 27
NaeI GCC'GGC
Cuts at: 114 2572 114
Size: 2458 1933
NarI GG'CG_CC
Cuts at: 1822 2070 1822
Size: 248 4143
NcoI C'CATG_G
Cuts at: 107 228 260 1227 1242 107
Size: 121 32 967 15 3256
Fragments arranged by size:
3256 967 121 32 15
NdeI CA'TA_TG
Cuts at: 1032 1032
Size: 4391
NgoMIV G'CCGG_C
Cuts at: 112 2570 112
Size: 2458 1933
NspI r_CATG'y
Cuts at: 1444 1944 2851 1444
Size: 500 907 2984
Fragments arranged by size:
2984 907 500
PasI CC'CwG_GG
Cuts at: 1002 1002
Size: 4391
PciI A'CATG_T
Cuts at: 2847 2847
Size: 4391
PflMI CCAn_nnn'nTGG
Cuts at: 293 342 933 293
Size: 49 591 3751
Fragments arranged by size:
3751 591 49
PmeI GTTT'AAAC
Cuts at: 1390 1390
Size: 4391
PmlI CAC'GTG
Cuts at: 843 843
Size: 4391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 4359
PpuMI rG'GwC_Cy
Cuts at: 196 196
Size: 4391
PspOMI G'GGCC_C
Cuts at: 182 239 846 998 1377 182
Size: 57 607 152 379 3196
Fragments arranged by size:
3196 607 379 152 57
PstI C_TGCA'G
Cuts at: 1438 2123 1438
Size: 685 3706
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4391
PvuII CAG'CTG
Cuts at: 315 342 581 876 1200 1816 2176 315
Size: 27 239 295 324 616 360 2530
Fragments arranged by size:
2530 616 360 324 295 239 27
RsrII CG'GwC_CG
Cuts at: 2586 2586
Size: 4391
SacI G_AGCT'C
Cuts at: 1405 1405
Size: 4391
SalI G'TCGA_C
Cuts at: 1428 1428
Size: 4391
SapI GCTCTTCn'nnn_
Cuts at: 2414 2624 2414
Size: 210 4181
SbfI CC_TGCA'GG
Cuts at: 1438 1438
Size: 4391
ScaI AGT'ACT
Cuts at: 3908 3908
Size: 4391
SfcI C'TryA_G
Cuts at: 33 1301 1434 2119 3112 3303 33
Size: 1268 133 685 993 191 1121
Fragments arranged by size:
1268 1121 993 685 191 133
SfiI GGCCn_nnn'nGGCC
Cuts at: 247 619 247
Size: 372 4019
SfoI GGC'GCC
Cuts at: 1823 2071 1823
Size: 248 4143
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
SmaI CCC'GGG
Cuts at: 181 856 922 1161 1413 181
Size: 675 66 239 252 3159
Fragments arranged by size:
3159 675 252 239 66
SmlI C'TyrA_G
Cuts at: 946 1546 2461 2953 3215 946
Size: 600 915 492 262 2122
Fragments arranged by size:
2122 915 600 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4391
SphI G_CATG'C
Cuts at: 1444 1944 1444
Size: 500 3891
StuI AGG'CCT
Cuts at: 305 379 305
Size: 74 4317
StyI C'CwwG_G
Cuts at: 107 228 260 1227 1242 1370 1525 1879 107
Size: 121 32 967 15 128 155 354 2619
Fragments arranged by size:
2619 967 354 155 128 121 32 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1974 2107 2566 2775 1974
Size: 133 459 209 3590
Fragments arranged by size:
3590 459 209 133
TatI w'GTAC_w
Cuts at: 413 959 3906 413
Size: 546 2947 898
Fragments arranged by size:
2947 898 546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 188 220 827 859 3908 3940 188
Size: 32 607 32 3049 32 639
Fragments arranged by size:
3049 639 607 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2188 2188
Size: 4391
XbaI T'CTAG_A
Cuts at: 1422 1422
Size: 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 776 1886 114
Size: 662 1110 2619
Fragments arranged by size:
2619 1110 662
XmaI C'CCGG_G
Cuts at: 179 854 920 1159 1411 179
Size: 675 66 239 252 3159
Fragments arranged by size:
3159 675 252 239 66
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BsgI BsiWI BssHII
BstEII BstZ17I ClaI CspCI EcoNI EcoRV FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PfoI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI