Restriction map for
pF1KB7203
(Circular) (Six-base) MAPSORT of: pF1KB7203.seq Check: 8565 from: 1 to: 4982
pF1KB7203 4982 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 2020 2020
Size: 4982
Acc65I G'GTAC_C
Cuts at: 1998 1998
Size: 4982
AclI AA'CG_TT
Cuts at: 4822 4822
Size: 4982
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 819 1467 1747 2807 3239 3986 4254 327
Size: 492 648 280 1060 432 747 268 1055
Fragments arranged by size:
1060 1055 747 648 492 432 280 268
AfeI AGC'GCT
Cuts at: 1771 1771
Size: 4982
AflIII A'CryG_T
Cuts at: 2334 3438 4415 2334
Size: 1104 977 2901
Fragments arranged by size:
2901 1104 977
AgeI A'CCGG_T
Cuts at: 655 3370 655
Size: 2715 2267
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 741 937 741
Size: 196 4786
AlwNI CAG_nnn'CTG
Cuts at: 1614 2287 3854 1614
Size: 673 1567 2742
Fragments arranged by size:
2742 1567 673
ApaI G_GGCC'C
Cuts at: 768 868 956 768
Size: 100 88 4794
Fragments arranged by size:
4794 100 88
ApaLI G'TGCA_C
Cuts at: 1003 3752 1003
Size: 2749 2233
ApoI r'AATT_y
Cuts at: 1986 4335 4591 1986
Size: 2349 256 2377
Fragments arranged by size:
2377 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4982
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
AvaI C'yCGr_G
Cuts at: 164 451 955 2002 164
Size: 287 504 1047 3144
Fragments arranged by size:
3144 1047 504 287
AvrII C'CTAG_G
Cuts at: 693 693
Size: 4982
BamHI G'GATC_C
Cuts at: 2007 2007
Size: 4982
BanI G'GyrC_C
Cuts at: 1455 1617 1998 2412 2660 2695 1455
Size: 162 381 414 248 35 3742
Fragments arranged by size:
3742 414 381 248 162 35
BanII G_rGCy'C
Cuts at: 401 629 645 768 868 875 956 1180 1361
Size: 228 16 123 100 7 81 224 181
Cuts at: 1361 1996 3026 401
Size: 635 1030 2357
Fragments arranged by size:
2357 1030 635 228 224 181 123 100
81 16 7
BbeI G_GCGC'C
Cuts at: 2416 2664 2416
Size: 248 4734
BbsI GAAGACnn'nnnn_
Cuts at: 1552 1552
Size: 4982
BbvCI CC'TCA_GC
Cuts at: 391 551 391
Size: 160 4822
BciVI GTATCCnnnnn_n'
Cuts at: 2880 3641 4982 2880
Size: 761 1341 2880
Fragments arranged by size:
2880 1341 761
BclI T'GATC_A
Cuts at: 1703 2502 1703
Size: 799 4183
BglI GCCn_nnn'nGGC
Cuts at: 829 829
Size: 4982
BglII A'GATC_T
Cuts at: 2497 4534 2497
Size: 2037 2945
BlpI GC'TnA_GC
Cuts at: 2094 2094
Size: 4982
Bme1580I G_kGCm'C
Cuts at: 548 768 868 956 1007 1304 1622 2607 2700
Size: 220 100 88 51 297 318 985 93
Cuts at: 2700 3756 548
Size: 1056 1774
Fragments arranged by size:
1774 1056 985 318 297 220 100 93
88 51
BmrI ACTGGGnnnn_n'
Cuts at: 1574 1846 2194 2275 2610 4754 1574
Size: 272 348 81 335 2144 1802
Fragments arranged by size:
2144 1802 348 335 272 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1055 3349 1055
Size: 2294 2688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 325 382 579 589 670 962 987 1002
Size: 49 57 197 10 81 292 25 15
Cuts at: 1002 276
Size: 4256
Fragments arranged by size:
4256 292 197 81 57 49 25 15
10
Bpu10I CC'TnA_GC
Cuts at: 391 487 551 391
Size: 96 64 4822
Fragments arranged by size:
4822 96 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1348 2158 3037 3529 3827 1348
Size: 810 879 492 298 2503
Fragments arranged by size:
2503 879 810 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 646 4686 646
Size: 4040 942
BsaAI yAC'GTr
Cuts at: 814 2335 814
Size: 1521 3461
BsaBI GATnn'nnATC
Cuts at: 6 2496 4533 6
Size: 2490 2037 455
Fragments arranged by size:
2490 2037 455
BsaHI Gr'CG_yC
Cuts at: 271 429 494 2413 2661 3363 4859 271
Size: 158 65 1919 248 702 1496 394
Fragments arranged by size:
1919 1496 702 394 248 158 65
BsaWI w'CCGG_w
Cuts at: 655 1075 2171 2692 3370 3644 3791 4202 655
Size: 420 1096 521 678 274 147 411 1435
Fragments arranged by size:
1435 1096 678 521 420 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 355 385 1057 1087 1780 1810 1852 1882 355
Size: 30 672 30 693 30 42 30 3455
Fragments arranged by size:
3455 693 672 42 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 984 1330 1876 984
Size: 346 546 4090
Fragments arranged by size:
4090 546 346
BseYI C'CCAG_C
Cuts at: 761 1044 1303 2407 3165 3742 4238 761
Size: 283 259 1104 758 577 496 1505
Fragments arranged by size:
1505 1104 758 577 496 283 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 1627 1555
Size: 72 4910
BsiEI CG_ry'CG
Cuts at: 104 2524 2570 3778 104
Size: 2420 46 1208 1308
Fragments arranged by size:
2420 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 401 629 875 1007 1188 1334 1361 1761 1996
Size: 228 246 132 181 146 27 400 235
Cuts at: 1996 2774 2964 3756 401
Size: 778 190 792 1627
Fragments arranged by size:
1627 792 778 400 246 235 228 190
181 146 132 27
BsmBI CGTCTCn'nnnn_
Cuts at: 639 639
Size: 4982
Bsp1286I G_dGCh'C
Cuts at: 401 548 629 645 768 868 875 956 1007
Size: 147 81 16 123 100 7 81 51
Cuts at: 1007 1180 1188 1304 1334 1361 1622 1761 1996
Size: 173 8 116 30 27 261 139 235
Cuts at: 1996 2607 2700 2774 2964 3026 3756 401
Size: 611 93 74 190 62 730 1627
Fragments arranged by size:
1627 730 611 261 235 190 173 147
139 123 116 100 93 81 81 74
62 51 30 27 16 8 7
BspEI T'CCGG_A
Cuts at: 1075 4202 1075
Size: 3127 1855
BspHI T'CATG_A
Cuts at: 4158 4158
Size: 4982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 527 2032 2246 2548 2929 527
Size: 1505 214 302 381 2580
Fragments arranged by size:
2580 1505 381 302 214
BsrBI CCG'CTC
Cuts at: 50 309 3274 3328 4812 4977 50
Size: 259 2965 54 1484 165 55
Fragments arranged by size:
2965 1484 259 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1721 2894 1721
Size: 1173 3809
BsrFI r'CCGG_y
Cuts at: 655 2980 3161 3370 655
Size: 2325 181 209 2267
Fragments arranged by size:
2325 2267 209 181
BssHII G'CGCG_C
Cuts at: 559 559
Size: 4982
BssSI C'ACGA_G
Cuts at: 1029 3253 3611 1029
Size: 2224 358 2400
Fragments arranged by size:
2400 2224 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1518 2359 1518
Size: 841 4141
BstBI TT'CG_AA
Cuts at: 3343 3343
Size: 4982
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1590 1659 114
Size: 1476 69 3437
Fragments arranged by size:
3437 1476 69
BstYI r'GATC_y
Cuts at: 1 2007 2474 2497 2832 3078 4090 4176 4188
Size: 2006 467 23 335 246 1012 86 12
Cuts at: 4188 4534 1
Size: 346 449
Fragments arranged by size:
2006 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2932 3113 3276 2932
Size: 181 163 4638
Fragments arranged by size:
4638 181 163
BtsI GCAGTG_nn'
Cuts at: 919 1064 1639 1761 2225 919
Size: 145 575 122 464 3676
Fragments arranged by size:
3676 575 464 145 122
DraI TTT'AAA
Cuts at: 1981 1981
Size: 4982
DraIII CAC_nnn'GTG
Cuts at: 1437 1437
Size: 4982
DrdI GACnn_nn'nnGTC
Cuts at: 2688 3546 2688
Size: 858 4124
EaeI y'GGCC_r
Cuts at: 314 464 672 821 887 1244 1283 1712 2567
Size: 150 208 149 66 357 39 429 855
Cuts at: 2567 2741 3132 3159 4504 314
Size: 174 391 27 1345 792
Fragments arranged by size:
1345 855 792 429 391 357 208 174
150 149 66 39 27
EagI C'GGCC_G
Cuts at: 2567 2567
Size: 4982
EarI CTCTTCn'nnn_
Cuts at: 1766 3005 3215 1766
Size: 1239 210 3533
Fragments arranged by size:
3533 1239 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1223 3190 3500 3646 4792 1223
Size: 1967 310 146 1146 1413
Fragments arranged by size:
1967 1413 1146 310 146
EcoICRI GAG'CTC
Cuts at: 399 627 873 1359 1994 399
Size: 228 246 486 635 3387
Fragments arranged by size:
3387 635 486 246 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 325 327 382 579 589 670 819 962
Size: 49 2 55 197 10 81 149 143
Cuts at: 962 987 1002 1467 1747 2807 3239 3986 4254
Size: 25 15 465 280 1060 432 747 268
Cuts at: 4254 276
Size: 1004
Fragments arranged by size:
1060 1004 747 465 432 280 268 197
149 143 81 55 49 25 15 10
2
EcoO109I rG'GnC_Cy
Cuts at: 347 420 690 765 864 899 986 1378 1611
Size: 73 270 75 99 35 87 392 233
Cuts at: 1611 2121 347
Size: 510 3208
Fragments arranged by size:
3208 510 392 270 233 99 87 75
73 35
EcoRI G'AATT_C
Cuts at: 1986 1986
Size: 4982
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3001 3001
Size: 4982
FspI TGC'GCA
Cuts at: 2763 2763
Size: 4982
HaeII r_GCGC'y
Cuts at: 487 1773 2416 2664 3686 4665 487
Size: 1286 643 248 1022 979 804
Fragments arranged by size:
1286 1022 979 804 643 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1070 1102 1544 1576 1852 1884 2824 2854 2856
Size: 32 442 32 276 32 940 30 2
Cuts at: 2856 2886 1070
Size: 30 3166
Fragments arranged by size:
3166 940 442 276 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1824 2021 1824
Size: 197 4785
KasI G'GCGC_C
Cuts at: 2412 2660 2412
Size: 248 4734
KpnI G_GTAC'C
Cuts at: 2002 2002
Size: 4982
MluI A'CGCG_T
Cuts at: 4415 4415
Size: 4982
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3653 3837 3653
Size: 184 4798
MscI TGG'CCA
Cuts at: 889 1246 1285 1714 2743 889
Size: 357 39 429 1029 3128
Fragments arranged by size:
3128 1029 429 357 39
MslI CAynn'nnrTG
Cuts at: 112 2971 3098 112
Size: 2859 127 1996
Fragments arranged by size:
2859 1996 127
MspA1I CmG'CkG
Cuts at: 191 369 550 606 1276 2093 2407 2767 3780
Size: 178 181 56 670 817 314 360 1013
Cuts at: 3780 4025 191
Size: 245 1148
Fragments arranged by size:
1148 1013 817 670 360 314 245 181
178 56
NaeI GCC'GGC
Cuts at: 3163 3163
Size: 4982
NarI GG'CG_CC
Cuts at: 2413 2661 2413
Size: 248 4734
NgoMIV G'CCGG_C
Cuts at: 3161 3161
Size: 4982
NspI r_CATG'y
Cuts at: 129 722 1255 1521 2035 2535 3442 129
Size: 593 533 266 514 500 907 1669
Fragments arranged by size:
1669 907 593 533 514 500 266
PasI CC'CwG_GG
Cuts at: 351 476 713 949 1350 351
Size: 125 237 236 401 3983
Fragments arranged by size:
3983 401 237 236 125
PciI A'CATG_T
Cuts at: 3438 3438
Size: 4982
PflMI CCAn_nnn'nTGG
Cuts at: 207 207
Size: 4982
PfoI T'CCnGG_A
Cuts at: 566 879 566
Size: 313 4669
PmeI GTTT'AAAC
Cuts at: 1981 1981
Size: 4982
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 4121
Size: 32 4950
PpuMI rG'GwC_Cy
Cuts at: 690 899 1378 690
Size: 209 479 4294
Fragments arranged by size:
4294 479 209
PspOMI G'GGCC_C
Cuts at: 764 864 952 764
Size: 100 88 4794
Fragments arranged by size:
4794 100 88
PstI C_TGCA'G
Cuts at: 196 524 1059 1756 2029 2714 196
Size: 328 535 697 273 685 2464
Fragments arranged by size:
2464 697 685 535 328 273
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4982
PvuII CAG'CTG
Cuts at: 191 369 606 2407 2767 191
Size: 178 237 1801 360 2406
Fragments arranged by size:
2406 1801 360 237 178
RsrII CG'GwC_CG
Cuts at: 3177 3177
Size: 4982
SacI G_AGCT'C
Cuts at: 401 629 875 1361 1996 401
Size: 228 246 486 635 3387
Fragments arranged by size:
3387 635 486 246 228
SalI G'TCGA_C
Cuts at: 2019 2019
Size: 4982
SapI GCTCTTCn'nnn_
Cuts at: 1766 3005 3215 1766
Size: 1239 210 3533
Fragments arranged by size:
3533 1239 210
SbfI CC_TGCA'GG
Cuts at: 524 2029 524
Size: 1505 3477
ScaI AGT'ACT
Cuts at: 1969 4499 1969
Size: 2530 2452
SexAI A'CCwGG_T
Cuts at: 740 740
Size: 4982
SfcI C'TryA_G
Cuts at: 33 192 520 700 1055 1752 1842 2025 2710
Size: 159 328 180 355 697 90 183 685
Cuts at: 2710 3703 3894 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 697 685 355 328 191 183
180 159 90
SfiI GGCCn_nnn'nGGCC
Cuts at: 829 829
Size: 4982
SfoI GGC'GCC
Cuts at: 2414 2662 2414
Size: 248 4734
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
SmaI CCC'GGG
Cuts at: 166 453 2004 166
Size: 287 1551 3144
Fragments arranged by size:
3144 1551 287
SmlI C'TyrA_G
Cuts at: 1327 2137 3052 3544 3806 1327
Size: 810 915 492 262 2503
Fragments arranged by size:
2503 915 810 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4982
SphI G_CATG'C
Cuts at: 129 1521 2035 2535 129
Size: 1392 514 500 2576
Fragments arranged by size:
2576 1392 514 500
StuI AGG'CCT
Cuts at: 340 1933 340
Size: 1593 3389
StyI C'CwwG_G
Cuts at: 693 1442 2116 2470 693
Size: 749 674 354 3205
Fragments arranged by size:
3205 749 674 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 199 898 2565 2698 3157 3366 199
Size: 699 1667 133 459 209 1815
Fragments arranged by size:
1815 1667 699 459 209 133
TatI w'GTAC_w
Cuts at: 1967 4497 1967
Size: 2530 2452
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 355 387 1057 1089 1283 1315 1423 1455 4499
Size: 32 670 32 194 32 108 32 3044
Cuts at: 4499 4531 355
Size: 32 806
Fragments arranged by size:
3044 806 670 194 108 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2779 2779
Size: 4982
XbaI T'CTAG_A
Cuts at: 2013 2013
Size: 4982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 300 1104 1187 1656 1659 2477 300
Size: 804 83 469 3 818 2805
Fragments arranged by size:
2805 818 804 469 83 3
XmaI C'CCGG_G
Cuts at: 164 451 2002 164
Size: 287 1551 3144
Fragments arranged by size:
3144 1551 287
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BstEII
BstZ17I Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI