Restriction map for
pF1KB7202
(Circular) (Six-base) MAPSORT of: pF1KB7202.seq Check: 3557 from: 1 to: 3836
pF1KB7202 3836 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 874 874
Size: 3836
Acc65I G'GTAC_C
Cuts at: 852 852
Size: 3836
AclI AA'CG_TT
Cuts at: 3676 3676
Size: 3836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1661 2093 2840 3108 1661
Size: 432 747 268 2389
Fragments arranged by size:
2389 747 432 268
AflIII A'CryG_T
Cuts at: 1188 2292 3269 1188
Size: 1104 977 1755
Fragments arranged by size:
1755 1104 977
AgeI A'CCGG_T
Cuts at: 2224 2224
Size: 3836
AlwNI CAG_nnn'CTG
Cuts at: 537 1141 2708 537
Size: 604 1567 1665
Fragments arranged by size:
1665 1567 604
ApaI G_GGCC'C
Cuts at: 153 153
Size: 3836
ApaLI G'TGCA_C
Cuts at: 2606 2606
Size: 3836
ApoI r'AATT_y
Cuts at: 840 3189 3445 840
Size: 2349 256 1231
Fragments arranged by size:
2349 1231 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
AvaI C'yCGr_G
Cuts at: 144 414 502 856 144
Size: 270 88 354 3124
Fragments arranged by size:
3124 354 270 88
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 360 393 360
Size: 33 3803
BamHI G'GATC_C
Cuts at: 861 861
Size: 3836
BanI G'GyrC_C
Cuts at: 246 608 852 1266 1514 1549 246
Size: 362 244 414 248 35 2533
Fragments arranged by size:
2533 414 362 248 244 35
BanII G_rGCy'C
Cuts at: 153 395 671 698 850 1880 153
Size: 242 276 27 152 1030 2109
Fragments arranged by size:
2109 1030 276 242 152 27
BbeI G_GCGC'C
Cuts at: 612 1270 1518 612
Size: 658 248 2930
Fragments arranged by size:
2930 658 248
BbvCI CC'TCA_GC
Cuts at: 763 763
Size: 3836
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 610 644 610
Size: 34 3802
BciVI GTATCCnnnnn_n'
Cuts at: 388 536 1734 2495 3836 388
Size: 148 1198 761 1341 388
Fragments arranged by size:
1341 1198 761 388 148
BclI T'GATC_A
Cuts at: 1356 1356
Size: 3836
BglI GCCn_nnn'nGGC
Cuts at: 675 777 675
Size: 102 3734
BglII A'GATC_T
Cuts at: 1351 3388 1351
Size: 2037 1799
BlpI GC'TnA_GC
Cuts at: 948 948
Size: 3836
Bme1580I G_kGCm'C
Cuts at: 153 249 421 1461 1554 2610 153
Size: 96 172 1040 93 1056 1379
Fragments arranged by size:
1379 1056 1040 172 96 93
BmrI ACTGGGnnnn_n'
Cuts at: 583 1048 1129 1464 3608 583
Size: 465 81 335 2144 811
Fragments arranged by size:
2144 811 465 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 3836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 409 322
Size: 87 3749
Bpu10I CC'TnA_GC
Cuts at: 763 763
Size: 3836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 1012 1891 2383 2681 420
Size: 592 879 492 298 1575
Fragments arranged by size:
1575 879 592 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3540 3540
Size: 3836
BsaAI yAC'GTr
Cuts at: 471 1189 471
Size: 718 3118
BsaBI GATnn'nnATC
Cuts at: 6 1350 3387 6
Size: 1344 2037 455
Fragments arranged by size:
2037 1344 455
BsaHI Gr'CG_yC
Cuts at: 285 609 1267 1515 2217 3713 285
Size: 324 658 248 702 1496 408
Fragments arranged by size:
1496 702 658 408 324 248
BsaWI w'CCGG_w
Cuts at: 1025 1546 2224 2498 2645 3056 1025
Size: 521 678 274 147 411 1805
Fragments arranged by size:
1805 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 315 345 315
Size: 30 3806
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 230 385 230
Size: 155 3681
BseYI C'CCAG_C
Cuts at: 236 250 474 530 549 556 1261 2019 2596
Size: 14 224 56 19 7 705 758 577
Cuts at: 2596 3092 236
Size: 496 980
Fragments arranged by size:
980 758 705 577 496 224 56 19
14 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 586
Size: 3836
BsiEI CG_ry'CG
Cuts at: 104 183 292 354 1378 1424 2632 104
Size: 79 109 62 1024 46 1208 1308
Fragments arranged by size:
1308 1208 1024 109 79 62 46
BsiHKAI G_wGCw'C
Cuts at: 395 569 850 1628 1818 2610 395
Size: 174 281 778 190 792 1621
Fragments arranged by size:
1621 792 778 281 190 174
Bsp1286I G_dGCh'C
Cuts at: 153 249 395 421 569 671 698 850 1461
Size: 96 146 26 148 102 27 152 611
Cuts at: 1461 1554 1628 1818 1880 2610 153
Size: 93 74 190 62 730 1379
Fragments arranged by size:
1379 730 611 190 152 148 146 102
96 93 74 62 27 26
BspEI T'CCGG_A
Cuts at: 3056 3056
Size: 3836
BspHI T'CATG_A
Cuts at: 3012 3012
Size: 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 886 1100 1402 1783 886
Size: 214 302 381 2939
Fragments arranged by size:
2939 381 302 214
BsrBI CCG'CTC
Cuts at: 50 314 2128 2182 3666 3831 50
Size: 264 1814 54 1484 165 55
Fragments arranged by size:
1814 1484 264 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1748 1748
Size: 3836
BsrFI r'CCGG_y
Cuts at: 776 1834 2015 2224 776
Size: 1058 181 209 2388
Fragments arranged by size:
2388 1058 209 181
BssSI C'ACGA_G
Cuts at: 442 2107 2465 442
Size: 1665 358 1813
Fragments arranged by size:
1813 1665 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1213 1213
Size: 3836
BstBI TT'CG_AA
Cuts at: 2197 2197
Size: 3836
BstYI r'GATC_y
Cuts at: 1 744 861 1328 1351 1686 1932 2944 3030
Size: 743 117 467 23 335 246 1012 86
Cuts at: 3030 3042 3388 1
Size: 12 346 449
Fragments arranged by size:
1012 743 467 449 346 335 246 117
86 23 12
BtgI C'CryG_G
Cuts at: 107 513 107
Size: 406 3430
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 1967 2130 1786
Size: 181 163 3492
Fragments arranged by size:
3492 181 163
BtsI GCAGTG_nn'
Cuts at: 1079 1079
Size: 3836
DraI TTT'AAA
Cuts at: 835 835
Size: 3836
DrdI GACnn_nn'nnGTC
Cuts at: 1542 2400 1542
Size: 858 2978
EaeI y'GGCC_r
Cuts at: 180 351 1421 1595 1986 2013 3358 180
Size: 171 1070 174 391 27 1345 658
Fragments arranged by size:
1345 1070 658 391 174 171 27
EagI C'GGCC_G
Cuts at: 180 351 1421 180
Size: 171 1070 2595
Fragments arranged by size:
2595 1070 171
EarI CTCTTCn'nnn_
Cuts at: 312 686 1859 2069 312
Size: 374 1173 210 2079
Fragments arranged by size:
2079 1173 374 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2044 2354 2500 3646 2044
Size: 310 146 1146 2234
Fragments arranged by size:
2234 1146 310 146
EcoICRI GAG'CTC
Cuts at: 393 848 393
Size: 455 3381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 409 1661 2093 2840 3108 322
Size: 87 1252 432 747 268 1050
Fragments arranged by size:
1252 1050 747 432 268 87
EcoNI CCTnn'n_nnAGG
Cuts at: 718 718
Size: 3836
EcoO109I rG'GnC_Cy
Cuts at: 149 270 317 485 498 711 770 975 149
Size: 121 47 168 13 213 59 205 3010
Fragments arranged by size:
3010 213 205 168 121 59 47 13
EcoRI G'AATT_C
Cuts at: 840 840
Size: 3836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3836
FspI TGC'GCA
Cuts at: 1617 1617
Size: 3836
HaeII r_GCGC'y
Cuts at: 557 612 1270 1518 2540 3519 557
Size: 55 658 248 1022 979 874
Fragments arranged by size:
1022 979 874 658 248 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1678 1708 1710 1740 1678
Size: 30 2 30 3774
Fragments arranged by size:
3774 30 30 2
HincII GTy'rAC
Cuts at: 875 875
Size: 3836
KasI G'GCGC_C
Cuts at: 608 1266 1514 608
Size: 658 248 2930
Fragments arranged by size:
2930 658 248
KpnI G_GTAC'C
Cuts at: 856 856
Size: 3836
MluI A'CGCG_T
Cuts at: 3269 3269
Size: 3836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2507 2691 2507
Size: 184 3652
MscI TGG'CCA
Cuts at: 1597 1597
Size: 3836
MslI CAynn'nnrTG
Cuts at: 112 1825 1952 112
Size: 1713 127 1996
Fragments arranged by size:
1996 1713 127
MspA1I CmG'CkG
Cuts at: 126 135 236 546 790 947 1261 1621 2634
Size: 9 101 310 244 157 314 360 1013
Cuts at: 2634 2879 126
Size: 245 1083
Fragments arranged by size:
1083 1013 360 314 310 245 244 157
101 9
NaeI GCC'GGC
Cuts at: 778 2017 778
Size: 1239 2597
NarI GG'CG_CC
Cuts at: 609 1267 1515 609
Size: 658 248 2930
Fragments arranged by size:
2930 658 248
NcoI C'CATG_G
Cuts at: 107 513 107
Size: 406 3430
NgoMIV G'CCGG_C
Cuts at: 776 2015 776
Size: 1239 2597
NspI r_CATG'y
Cuts at: 889 1389 2296 889
Size: 500 907 2429
Fragments arranged by size:
2429 907 500
PasI CC'CwG_GG
Cuts at: 163 187 163
Size: 24 3812
PciI A'CATG_T
Cuts at: 2292 2292
Size: 3836
PflMI CCAn_nnn'nTGG
Cuts at: 236 236
Size: 3836
PmeI GTTT'AAAC
Cuts at: 835 835
Size: 3836
PmlI CAC'GTG
Cuts at: 471 471
Size: 3836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3804
PpuMI rG'GwC_Cy
Cuts at: 317 485 711 317
Size: 168 226 3442
Fragments arranged by size:
3442 226 168
PspOMI G'GGCC_C
Cuts at: 149 149
Size: 3836
PstI C_TGCA'G
Cuts at: 125 883 1568 125
Size: 758 685 2393
Fragments arranged by size:
2393 758 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3836
PvuII CAG'CTG
Cuts at: 126 1261 1621 126
Size: 1135 360 2341
Fragments arranged by size:
2341 1135 360
RsrII CG'GwC_CG
Cuts at: 2031 2031
Size: 3836
SacI G_AGCT'C
Cuts at: 395 850 395
Size: 455 3381
SalI G'TCGA_C
Cuts at: 873 873
Size: 3836
SanDI GG'GwC_CC
Cuts at: 317 317
Size: 3836
SapI GCTCTTCn'nnn_
Cuts at: 686 1859 2069 686
Size: 1173 210 2453
Fragments arranged by size:
2453 1173 210
SbfI CC_TGCA'GG
Cuts at: 883 883
Size: 3836
ScaI AGT'ACT
Cuts at: 3353 3353
Size: 3836
SfcI C'TryA_G
Cuts at: 33 121 879 1564 2557 2748 33
Size: 88 758 685 993 191 1121
Fragments arranged by size:
1121 993 758 685 191 88
SfoI GGC'GCC
Cuts at: 610 1268 1516 610
Size: 658 248 2930
Fragments arranged by size:
2930 658 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
SmaI CCC'GGG
Cuts at: 504 858 504
Size: 354 3482
SmlI C'TyrA_G
Cuts at: 144 399 991 1906 2398 2660 144
Size: 255 592 915 492 262 1320
Fragments arranged by size:
1320 915 592 492 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3836
SphI G_CATG'C
Cuts at: 889 1389 889
Size: 500 3336
StyI C'CwwG_G
Cuts at: 107 153 513 970 1324 107
Size: 46 360 457 354 2619
Fragments arranged by size:
2619 457 360 354 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 264 306 1419 1552 2011 2220 264
Size: 42 1113 133 459 209 1880
Fragments arranged by size:
1880 1113 459 209 133 42
TatI w'GTAC_w
Cuts at: 3351 3351
Size: 3836
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 239 271 315 347 3353 3385 239
Size: 32 44 32 3006 32 690
Fragments arranged by size:
3006 690 44 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1633 1633
Size: 3836
XbaI T'CTAG_A
Cuts at: 867 867
Size: 3836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 160 1331 160
Size: 1171 2665
XhoI C'TCGA_G
Cuts at: 144 144
Size: 3836
XmaI C'CCGG_G
Cuts at: 502 856 502
Size: 354 3482
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbsI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI ZraI