Restriction map for
pF1KB7199
(Circular) (Six-base) MAPSORT of: pF1KB7199.seq Check: 4681 from: 1 to: 4514
pF1KB7199 4514 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1552 1552
Size: 4514
Acc65I G'GTAC_C
Cuts at: 1530 1530
Size: 4514
AclI AA'CG_TT
Cuts at: 4354 4354
Size: 4514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 906 2339 2771 3518 3786 293
Size: 613 1433 432 747 268 1021
Fragments arranged by size:
1433 1021 747 613 432 268
AflIII A'CryG_T
Cuts at: 948 1866 2970 3947 948
Size: 918 1104 977 1515
Fragments arranged by size:
1515 1104 977 918
AgeI A'CCGG_T
Cuts at: 2902 2902
Size: 4514
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4514
AlwNI CAG_nnn'CTG
Cuts at: 858 1819 3386 858
Size: 961 1567 1986
Fragments arranged by size:
1986 1567 961
ApaI G_GGCC'C
Cuts at: 815 976 815
Size: 161 4353
ApaLI G'TGCA_C
Cuts at: 1069 3284 1069
Size: 2215 2299
ApoI r'AATT_y
Cuts at: 1189 1518 3867 4123 1189
Size: 329 2349 256 1580
Fragments arranged by size:
2349 1580 329 256
AscI GG'CGCG_CC
Cuts at: 167 167
Size: 4514
AseI AT'TA_AT
Cuts at: 20 20
Size: 4514
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
AvaI C'yCGr_G
Cuts at: 501 568 595 1016 1104 1305 1534 501
Size: 67 27 421 88 201 229 3481
Fragments arranged by size:
3481 421 229 201 88 67 27
BamHI G'GATC_C
Cuts at: 1539 1539
Size: 4514
BanI G'GyrC_C
Cuts at: 408 620 817 1530 1944 2192 2227 408
Size: 212 197 713 414 248 35 2695
Fragments arranged by size:
2695 713 414 248 212 197 35
BanII G_rGCy'C
Cuts at: 286 322 488 524 575 815 976 1312 1528
Size: 36 166 36 51 240 161 336 216
Cuts at: 1528 2558 286
Size: 1030 2242
Fragments arranged by size:
2242 1030 336 240 216 166 161 51
36 36
BbeI G_GCGC'C
Cuts at: 821 1948 2196 821
Size: 1127 248 3139
Fragments arranged by size:
3139 1127 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 528 562 855 889 1296 1330 528
Size: 34 293 34 407 34 3712
Fragments arranged by size:
3712 407 293 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2412 3173 4514 2412
Size: 761 1341 2412
Fragments arranged by size:
2412 1341 761
BclI T'GATC_A
Cuts at: 2034 2034
Size: 4514
BfrBI ATG'CAT
Cuts at: 933 1245 933
Size: 312 4202
BglI GCCn_nnn'nGGC
Cuts at: 535 636 535
Size: 101 4413
BglII A'GATC_T
Cuts at: 1118 2029 4066 1118
Size: 911 2037 1566
Fragments arranged by size:
2037 1566 911
BlpI GC'TnA_GC
Cuts at: 280 884 889 1626 280
Size: 604 5 737 3168
Fragments arranged by size:
3168 737 604 5
Bme1580I G_kGCm'C
Cuts at: 413 623 641 815 976 1015 1073 2139 2232
Size: 210 18 174 161 39 58 1066 93
Cuts at: 2232 3288 413
Size: 1056 1639
Fragments arranged by size:
1639 1066 1056 210 174 161 93 58
39 18
BmgBI CAC'GTC
Cuts at: 993 993
Size: 4514
BmrI ACTGGGnnnn_n'
Cuts at: 703 1011 1726 1807 2142 4286 703
Size: 308 715 81 335 2144 931
Fragments arranged by size:
2144 931 715 335 308 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 516 2881 516
Size: 2365 2149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 539
Size: 4514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 1328 1690 2569 3061 3359 1126
Size: 202 362 879 492 298 2281
Fragments arranged by size:
2281 879 492 362 298 202
BsaI GGTCTCn'nnnn_
Cuts at: 4218 4218
Size: 4514
BsaAI yAC'GTr
Cuts at: 642 1867 642
Size: 1225 3289
BsaBI GATnn'nnATC
Cuts at: 6 2028 4065 6
Size: 2022 2037 455
Fragments arranged by size:
2037 2022 455
BsaHI Gr'CG_yC
Cuts at: 818 1945 2193 2895 4391 818
Size: 1127 248 702 1496 941
Fragments arranged by size:
1496 1127 941 702 248
BsaWI w'CCGG_w
Cuts at: 914 1703 2224 2902 3176 3323 3734 914
Size: 789 521 678 274 147 411 1694
Fragments arranged by size:
1694 789 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 779 809 847 877 1012 1042 1294 1324 779
Size: 30 38 30 135 30 252 30 3969
Fragments arranged by size:
3969 252 135 38 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1021 1021
Size: 4514
BseYI C'CCAG_C
Cuts at: 147 412 616 1939 2697 3274 3770 147
Size: 265 204 1323 758 577 496 891
Fragments arranged by size:
1323 891 758 577 496 265 204
BsiEI CG_ry'CG
Cuts at: 104 690 743 915 1230 2056 2102 3310 104
Size: 586 53 172 315 826 46 1208 1308
Fragments arranged by size:
1308 1208 826 586 315 172 53 46
BsiHKAI G_wGCw'C
Cuts at: 322 1073 1312 1528 2306 2496 3288 322
Size: 751 239 216 778 190 792 1548
Fragments arranged by size:
1548 792 778 751 239 216 190
Bsp1286I G_dGCh'C
Cuts at: 286 322 413 488 524 575 623 641 815
Size: 36 91 75 36 51 48 18 174
Cuts at: 815 976 1015 1073 1312 1528 2139 2232 2306
Size: 161 39 58 239 216 611 93 74
Cuts at: 2306 2496 2558 3288 286
Size: 190 62 730 1512
Fragments arranged by size:
1512 730 611 239 216 190 174 161
93 91 75 74 62 58 51 48
39 36 36 18
BspEI T'CCGG_A
Cuts at: 3734 3734
Size: 4514
BspHI T'CATG_A
Cuts at: 1335 3690 1335
Size: 2355 2159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1564 1778 2080 2461 1564
Size: 214 302 381 3617
Fragments arranged by size:
3617 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2806 2860 4344 4509 50
Size: 2756 54 1484 165 55
Fragments arranged by size:
2756 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2426 2426
Size: 4514
BsrFI r'CCGG_y
Cuts at: 201 529 742 893 2512 2693 2902 201
Size: 328 213 151 1619 181 209 1813
Fragments arranged by size:
1813 1619 328 213 209 181 151
BssHII G'CGCG_C
Cuts at: 167 167
Size: 4514
BssSI C'ACGA_G
Cuts at: 181 2785 3143 181
Size: 2604 358 1552
Fragments arranged by size:
2604 1552 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1283 1891 1283
Size: 608 3906
BstBI TT'CG_AA
Cuts at: 2875 2875
Size: 4514
BstXI CCAn_nnnn'nTGG
Cuts at: 360 1474 360
Size: 1114 3400
BstYI r'GATC_y
Cuts at: 1 1118 1145 1539 2006 2029 2364 2610 3622
Size: 1117 27 394 467 23 335 246 1012
Cuts at: 3622 3708 3720 4066 1
Size: 86 12 346 449
Fragments arranged by size:
1117 1012 467 449 394 346 335 246
86 27 23 12
BtgI C'CryG_G
Cuts at: 290 807 956 290
Size: 517 149 3848
Fragments arranged by size:
3848 517 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2464 2645 2808 2464
Size: 181 163 4170
Fragments arranged by size:
4170 181 163
BtsI GCAGTG_nn'
Cuts at: 1757 1757
Size: 4514
DraI TTT'AAA
Cuts at: 1513 1513
Size: 4514
DrdI GACnn_nn'nnGTC
Cuts at: 2220 3078 2220
Size: 858 3656
EaeI y'GGCC_r
Cuts at: 687 713 740 2099 2273 2664 2691 4036 687
Size: 26 27 1359 174 391 27 1345 1165
Fragments arranged by size:
1359 1345 1165 391 174 27 27 26
EagI C'GGCC_G
Cuts at: 687 740 2099 687
Size: 53 1359 3102
Fragments arranged by size:
3102 1359 53
EarI CTCTTCn'nnn_
Cuts at: 529 898 2537 2747 529
Size: 369 1639 210 2296
Fragments arranged by size:
2296 1639 369 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 457 2722 3032 3178 4324 457
Size: 2265 310 146 1146 647
Fragments arranged by size:
2265 1146 647 310 146
EcoICRI GAG'CTC
Cuts at: 320 1310 1526 320
Size: 990 216 3308
Fragments arranged by size:
3308 990 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 539 906 2339 2771 3518 3786 293
Size: 246 367 1433 432 747 268 1021
Fragments arranged by size:
1433 1021 747 432 367 268 246
EcoO109I rG'GnC_Cy
Cuts at: 972 1157 1653 972
Size: 185 496 3833
Fragments arranged by size:
3833 496 185
EcoRI G'AATT_C
Cuts at: 1518 1518
Size: 4514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4514
FspI TGC'GCA
Cuts at: 1318 2295 1318
Size: 977 3537
HaeII r_GCGC'y
Cuts at: 428 617 749 821 1948 2196 3218 4197 428
Size: 189 132 72 1127 248 1022 979 745
Fragments arranged by size:
1127 1022 979 745 248 189 132 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1012 1044 2356 2386 2388 2418 1012
Size: 32 1312 30 2 30 3108
Fragments arranged by size:
3108 1312 32 30 30 2
HincII GTy'rAC
Cuts at: 1056 1553 1056
Size: 497 4017
KasI G'GCGC_C
Cuts at: 817 1944 2192 817
Size: 1127 248 3139
Fragments arranged by size:
3139 1127 248
KpnI G_GTAC'C
Cuts at: 1534 1534
Size: 4514
MluI A'CGCG_T
Cuts at: 3947 3947
Size: 4514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3185 3369 3185
Size: 184 4330
MscI TGG'CCA
Cuts at: 715 2275 715
Size: 1560 2954
MslI CAynn'nnrTG
Cuts at: 112 775 781 787 1066 1166 1244 1246 2503
Size: 663 6 6 279 100 78 2 1257
Cuts at: 2503 2630 112
Size: 127 1996
Fragments arranged by size:
1996 1257 663 279 127 100 78 6
6 2
MspA1I CmG'CkG
Cuts at: 268 292 559 888 1625 1939 2299 3312 3557
Size: 24 267 329 737 314 360 1013 245
Cuts at: 3557 268
Size: 1225
Fragments arranged by size:
1225 1013 737 360 329 314 267 245
24
NaeI GCC'GGC
Cuts at: 203 531 744 895 2695 203
Size: 328 213 151 1800 2022
Fragments arranged by size:
2022 1800 328 213 151
NarI GG'CG_CC
Cuts at: 818 1945 2193 818
Size: 1127 248 3139
Fragments arranged by size:
3139 1127 248
NcoI C'CATG_G
Cuts at: 956 956
Size: 4514
NgoMIV G'CCGG_C
Cuts at: 201 529 742 893 2693 201
Size: 328 213 151 1800 2022
Fragments arranged by size:
2022 1800 328 213 151
NsiI A_TGCA'T
Cuts at: 935 1247 935
Size: 312 4202
NspI r_CATG'y
Cuts at: 553 952 967 1245 1249 1567 2067 2974 553
Size: 399 15 278 4 318 500 907 2093
Fragments arranged by size:
2093 907 500 399 318 278 15 4
PasI CC'CwG_GG
Cuts at: 142 360 142
Size: 218 4296
PciI A'CATG_T
Cuts at: 948 2970 948
Size: 2022 2492
PfoI T'CCnGG_A
Cuts at: 119 119
Size: 4514
PmeI GTTT'AAAC
Cuts at: 1513 1513
Size: 4514
PmlI CAC'GTG
Cuts at: 642 642
Size: 4514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 448 480 3653 3685 448
Size: 32 3173 32 1277
Fragments arranged by size:
3173 1277 32 32
PpuMI rG'GwC_Cy
Cuts at: 1157 1157
Size: 4514
PshAI GACnn'nnGTC
Cuts at: 949 949
Size: 4514
PspOMI G'GGCC_C
Cuts at: 811 972 811
Size: 161 4353
PstI C_TGCA'G
Cuts at: 1449 1561 2246 1449
Size: 112 685 3717
Fragments arranged by size:
3717 685 112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4514
PvuII CAG'CTG
Cuts at: 559 888 1939 2299 559
Size: 329 1051 360 2774
Fragments arranged by size:
2774 1051 360 329
RsrII CG'GwC_CG
Cuts at: 2709 2709
Size: 4514
SacI G_AGCT'C
Cuts at: 322 1312 1528 322
Size: 990 216 3308
Fragments arranged by size:
3308 990 216
SacII CC_GC'GG
Cuts at: 293 293
Size: 4514
SalI G'TCGA_C
Cuts at: 1551 1551
Size: 4514
SapI GCTCTTCn'nnn_
Cuts at: 529 898 2537 2747 529
Size: 369 1639 210 2296
Fragments arranged by size:
2296 1639 369 210
SbfI CC_TGCA'GG
Cuts at: 1561 1561
Size: 4514
ScaI AGT'ACT
Cuts at: 4031 4031
Size: 4514
SexAI A'CCwGG_T
Cuts at: 653 653
Size: 4514
SfcI C'TryA_G
Cuts at: 33 762 1403 1445 1557 2242 3235 3426 33
Size: 729 641 42 112 685 993 191 1121
Fragments arranged by size:
1121 993 729 685 641 191 112 42
SfoI GGC'GCC
Cuts at: 819 1946 2194 819
Size: 1127 248 3139
Fragments arranged by size:
3139 1127 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
SmaI CCC'GGG
Cuts at: 597 1018 1106 1536 597
Size: 421 88 430 3575
Fragments arranged by size:
3575 430 421 88
SmlI C'TyrA_G
Cuts at: 1141 1343 1669 2584 3076 3338 1141
Size: 202 326 915 492 262 2317
Fragments arranged by size:
2317 915 492 326 262 202
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4514
SphI G_CATG'C
Cuts at: 553 1567 2067 553
Size: 1014 500 3000
Fragments arranged by size:
3000 1014 500
StyI C'CwwG_G
Cuts at: 956 1648 2002 956
Size: 692 354 3468
Fragments arranged by size:
3468 692 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2097 2230 2689 2898 2097
Size: 133 459 209 3713
Fragments arranged by size:
3713 459 209 133
TatI w'GTAC_w
Cuts at: 1043 4029 1043
Size: 2986 1528
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 779 811 847 879 1294 1326 4031 4063 779
Size: 32 36 32 415 32 2705 32 1230
Fragments arranged by size:
2705 1230 415 36 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2311 2311
Size: 4514
XbaI T'CTAG_A
Cuts at: 1545 1545
Size: 4514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 639 936 963 2009 639
Size: 297 27 1046 3144
Fragments arranged by size:
3144 1046 297 27
XmaI C'CCGG_G
Cuts at: 595 1016 1104 1534 595
Size: 421 88 430 3575
Fragments arranged by size:
3575 430 421 88
XmnI GAAnn'nnTTC
Cuts at: 223 1087 1177 223
Size: 864 90 3560
Fragments arranged by size:
3560 864 90
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AvrII
BaeI BbsI BbvCI BmtI Bpu10I BsgI BsiWI BsmI
BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PflMI PsiI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI