Restriction map for
pF1KB7196
(Circular) (Six-base) MAPSORT of: pF1KB7196.seq Check: 5134 from: 1 to: 4583
pF1KB7196 4583 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1621 1621
Size: 4583
Acc65I G'GTAC_C
Cuts at: 1599 1599
Size: 4583
AclI AA'CG_TT
Cuts at: 663 4423 663
Size: 3760 823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 2408 2840 3587 3855 1069
Size: 1339 432 747 268 1797
Fragments arranged by size:
1797 1339 747 432 268
AflIII A'CryG_T
Cuts at: 1092 1935 3039 4016 1092
Size: 843 1104 977 1659
Fragments arranged by size:
1659 1104 977 843
AgeI A'CCGG_T
Cuts at: 2971 2971
Size: 4583
AleI CACnn'nnGTG
Cuts at: 1285 1285
Size: 4583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 349 349
Size: 4583
AlwNI CAG_nnn'CTG
Cuts at: 1156 1888 3455 1156
Size: 732 1567 2284
Fragments arranged by size:
2284 1567 732
ApaLI G'TGCA_C
Cuts at: 1162 3353 1162
Size: 2191 2392
ApoI r'AATT_y
Cuts at: 504 949 1231 1306 1359 1587 3936 4192 504
Size: 445 282 75 53 228 2349 256 895
Fragments arranged by size:
2349 895 445 282 256 228 75 53
AseI AT'TA_AT
Cuts at: 20 20
Size: 4583
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
AvaI C'yCGr_G
Cuts at: 786 887 1603 786
Size: 101 716 3766
Fragments arranged by size:
3766 716 101
BamHI G'GATC_C
Cuts at: 111 743 1608 111
Size: 632 865 3086
Fragments arranged by size:
3086 865 632
BanI G'GyrC_C
Cuts at: 1599 2013 2261 2296 1599
Size: 414 248 35 3886
Fragments arranged by size:
3886 414 248 35
BanII G_rGCy'C
Cuts at: 178 1117 1597 2627 178
Size: 939 480 1030 2134
Fragments arranged by size:
2134 1030 939 480
BbeI G_GCGC'C
Cuts at: 2017 2265 2017
Size: 248 4335
BciVI GTATCCnnnnn_n'
Cuts at: 2481 3242 4583 2481
Size: 761 1341 2481
Fragments arranged by size:
2481 1341 761
BclI T'GATC_A
Cuts at: 911 2103 911
Size: 1192 3391
BglII A'GATC_T
Cuts at: 2098 4135 2098
Size: 2037 2546
BlpI GC'TnA_GC
Cuts at: 1695 1695
Size: 4583
Bme1580I G_kGCm'C
Cuts at: 1166 1261 2208 2301 3357 1166
Size: 95 947 93 1056 2392
Fragments arranged by size:
2392 1056 947 95 93
BmrI ACTGGGnnnn_n'
Cuts at: 211 1795 1876 2211 4355 211
Size: 1584 81 335 2144 439
Fragments arranged by size:
2144 1584 439 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 208 1049 2950 208
Size: 841 1901 1841
Fragments arranged by size:
1901 1841 841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 186
Size: 4583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1759 2638 3130 3428 1759
Size: 879 492 298 2914
Fragments arranged by size:
2914 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4287 4287
Size: 4583
BsaAI yAC'GTr
Cuts at: 1936 1936
Size: 4583
BsaBI GATnn'nnATC
Cuts at: 6 874 2097 4134 6
Size: 868 1223 2037 455
Fragments arranged by size:
2037 1223 868 455
BsaHI Gr'CG_yC
Cuts at: 2014 2262 2964 4460 2014
Size: 248 702 1496 2137
Fragments arranged by size:
2137 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1109 1772 2293 2971 3245 3392 3803 1109
Size: 663 521 678 274 147 411 1889
Fragments arranged by size:
1889 678 663 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 200 397 537 583 200
Size: 197 140 46 4200
Fragments arranged by size:
4200 197 140 46
BseYI C'CCAG_C
Cuts at: 997 2008 2766 3343 3839 997
Size: 1011 758 577 496 1741
Fragments arranged by size:
1741 1011 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2125 2171 3379 104
Size: 2021 46 1208 1308
Fragments arranged by size:
2021 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 134 178 627 1166 1597 2375 2565 3357 134
Size: 44 449 539 431 778 190 792 1360
Fragments arranged by size:
1360 792 778 539 449 431 190 44
Bsp1286I G_dGCh'C
Cuts at: 134 178 627 1117 1166 1261 1597 2208 2301
Size: 44 449 490 49 95 336 611 93
Cuts at: 2301 2375 2565 2627 3357 134
Size: 74 190 62 730 1360
Fragments arranged by size:
1360 730 611 490 449 336 190 95
93 74 62 49 44
BspEI T'CCGG_A
Cuts at: 3803 3803
Size: 4583
BspHI T'CATG_A
Cuts at: 491 3759 491
Size: 3268 1315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1633 1847 2149 2530 1633
Size: 214 302 381 3686
Fragments arranged by size:
3686 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2875 2929 4413 4578 50
Size: 2825 54 1484 165 55
Fragments arranged by size:
2825 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 459 639 2495 459
Size: 180 1856 2547
Fragments arranged by size:
2547 1856 180
BsrFI r'CCGG_y
Cuts at: 2581 2762 2971 2581
Size: 181 209 4193
Fragments arranged by size:
4193 209 181
BssSI C'ACGA_G
Cuts at: 1165 2854 3212 1165
Size: 1689 358 2536
Fragments arranged by size:
2536 1689 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1960 1960
Size: 4583
BstBI TT'CG_AA
Cuts at: 2944 2944
Size: 4583
BstYI r'GATC_y
Cuts at: 1 111 473 743 1608 2075 2098 2433 2679
Size: 110 362 270 865 467 23 335 246
Cuts at: 2679 3691 3777 3789 4135 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 865 467 449 362 346 335 270
246 110 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4583
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2533 2714 2877 2533
Size: 181 163 4239
Fragments arranged by size:
4239 181 163
BtsI GCAGTG_nn'
Cuts at: 974 1220 1826 974
Size: 246 606 3731
Fragments arranged by size:
3731 606 246
ClaI AT'CG_AT
Cuts at: 868 868
Size: 4583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 756 791 756
Size: 35 4548
DraI TTT'AAA
Cuts at: 1582 1582
Size: 4583
DraIII CAC_nnn'GTG
Cuts at: 598 598
Size: 4583
DrdI GACnn_nn'nnGTC
Cuts at: 2289 3147 2289
Size: 858 3725
EaeI y'GGCC_r
Cuts at: 2168 2342 2733 2760 4105 2168
Size: 174 391 27 1345 2646
Fragments arranged by size:
2646 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2168 2168
Size: 4583
EarI CTCTTCn'nnn_
Cuts at: 390 514 1076 2606 2816 390
Size: 124 562 1530 210 2157
Fragments arranged by size:
2157 1530 562 210 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2791 3101 3247 4393 2791
Size: 310 146 1146 2981
Fragments arranged by size:
2981 1146 310 146
EcoICRI GAG'CTC
Cuts at: 176 1595 176
Size: 1419 3164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 1069 2408 2840 3587 3855 186
Size: 883 1339 432 747 268 914
Fragments arranged by size:
1339 914 883 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 565 565
Size: 4583
EcoO109I rG'GnC_Cy
Cuts at: 1422 1503 1722 1422
Size: 81 219 4283
Fragments arranged by size:
4283 219 81
EcoRI G'AATT_C
Cuts at: 949 1587 949
Size: 638 3945
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4583
FspI TGC'GCA
Cuts at: 2364 2364
Size: 4583
HaeII r_GCGC'y
Cuts at: 2017 2265 3287 4266 2017
Size: 248 1022 979 2334
Fragments arranged by size:
2334 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 425 457 1019 1051 2425 2455 2457 2487 425
Size: 32 562 32 1374 30 2 30 2521
Fragments arranged by size:
2521 1374 562 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1622 1622
Size: 4583
KasI G'GCGC_C
Cuts at: 2013 2261 2013
Size: 248 4335
KpnI G_GTAC'C
Cuts at: 1603 1603
Size: 4583
MluI A'CGCG_T
Cuts at: 4016 4016
Size: 4583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3254 3438 3254
Size: 184 4399
MscI TGG'CCA
Cuts at: 2344 2344
Size: 4583
MslI CAynn'nnrTG
Cuts at: 331 344 1285 2572 2699 331
Size: 13 941 1287 127 2215
Fragments arranged by size:
2215 1287 941 127 13
MspA1I CmG'CkG
Cuts at: 557 982 1560 1694 2008 2368 3381 3626 557
Size: 425 578 134 314 360 1013 245 1514
Fragments arranged by size:
1514 1013 578 425 360 314 245 134
NaeI GCC'GGC
Cuts at: 2764 2764
Size: 4583
NarI GG'CG_CC
Cuts at: 2014 2262 2014
Size: 248 4335
NcoI C'CATG_G
Cuts at: 107 107
Size: 4583
NgoMIV G'CCGG_C
Cuts at: 2762 2762
Size: 4583
NspI r_CATG'y
Cuts at: 1125 1143 1636 2136 3043 1125
Size: 18 493 500 907 2665
Fragments arranged by size:
2665 907 500 493 18
PciI A'CATG_T
Cuts at: 3039 3039
Size: 4583
PflMI CCAn_nnn'nTGG
Cuts at: 223 223
Size: 4583
PfoI T'CCnGG_A
Cuts at: 1076 1076
Size: 4583
PmeI GTTT'AAAC
Cuts at: 1582 1582
Size: 4583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 4551
PpuMI rG'GwC_Cy
Cuts at: 1503 1503
Size: 4583
PshAI GACnn'nnGTC
Cuts at: 1334 1334
Size: 4583
PsiI TTA'TAA
Cuts at: 674 674
Size: 4583
PstI C_TGCA'G
Cuts at: 981 1630 2315 981
Size: 649 685 3249
Fragments arranged by size:
3249 685 649
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4583
PvuII CAG'CTG
Cuts at: 982 1560 2008 2368 982
Size: 578 448 360 3197
Fragments arranged by size:
3197 578 448 360
RsrII CG'GwC_CG
Cuts at: 2778 2778
Size: 4583
SacI G_AGCT'C
Cuts at: 178 1597 178
Size: 1419 3164
SalI G'TCGA_C
Cuts at: 1620 1620
Size: 4583
SapI GCTCTTCn'nnn_
Cuts at: 2606 2816 2606
Size: 210 4373
SbfI CC_TGCA'GG
Cuts at: 1630 1630
Size: 4583
ScaI AGT'ACT
Cuts at: 4100 4100
Size: 4583
SfcI C'TryA_G
Cuts at: 33 235 977 1626 2311 3304 3495 33
Size: 202 742 649 685 993 191 1121
Fragments arranged by size:
1121 993 742 685 649 202 191
SfoI GGC'GCC
Cuts at: 2015 2263 2015
Size: 248 4335
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
SmaI CCC'GGG
Cuts at: 788 1605 788
Size: 817 3766
SmlI C'TyrA_G
Cuts at: 1738 2653 3145 3407 1738
Size: 915 492 262 2914
Fragments arranged by size:
2914 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4583
SphI G_CATG'C
Cuts at: 1125 1636 2136 1125
Size: 511 500 3572
Fragments arranged by size:
3572 511 500
StyI C'CwwG_G
Cuts at: 107 584 1253 1717 2071 107
Size: 477 669 464 354 2619
Fragments arranged by size:
2619 669 477 464 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1070 2166 2299 2758 2967 1070
Size: 1096 133 459 209 2686
Fragments arranged by size:
2686 1096 459 209 133
TatI w'GTAC_w
Cuts at: 4098 4098
Size: 4583
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 335 367 4100 4132 335
Size: 32 3733 32 786
Fragments arranged by size:
3733 786 32 32
Tth111I GACn'n_nGTC
Cuts at: 2380 2380
Size: 4583
XbaI T'CTAG_A
Cuts at: 1614 1614
Size: 4583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 612 2078 612
Size: 1466 3117
XmaI C'CCGG_G
Cuts at: 786 1603 786
Size: 817 3766
XmnI GAAnn'nnTTC
Cuts at: 664 664
Size: 4583
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI