Restriction map for
pF1KB7194
(Circular) (Six-base) MAPSORT of: pF1KB7194.seq Check: 5666 from: 1 to: 5141
pF1KB7194 5141 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 756 756
Size: 5141
AatII G_ACGT'C
Cuts at: 1730 1730
Size: 5141
AccI GT'mk_AC
Cuts at: 437 830 1481 2051 2179 437
Size: 393 651 570 128 3399
Fragments arranged by size:
3399 651 570 393 128
Acc65I G'GTAC_C
Cuts at: 2157 2157
Size: 5141
AclI AA'CG_TT
Cuts at: 4981 4981
Size: 5141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 507 1186 2966 3398 4145 4413 507
Size: 679 1780 432 747 268 1235
Fragments arranged by size:
1780 1235 747 679 432 268
AfeI AGC'GCT
Cuts at: 148 148
Size: 5141
AflIII A'CryG_T
Cuts at: 825 2493 3597 4574 825
Size: 1668 1104 977 1392
Fragments arranged by size:
1668 1392 1104 977
AgeI A'CCGG_T
Cuts at: 3529 3529
Size: 5141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 788 1263 2098 788
Size: 475 835 3831
Fragments arranged by size:
3831 835 475
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1255 1287 1255
Size: 32 5109
AlwNI CAG_nnn'CTG
Cuts at: 813 891 1923 2446 4013 813
Size: 78 1032 523 1567 1941
Fragments arranged by size:
1941 1567 1032 523 78
ApaI G_GGCC'C
Cuts at: 2016 2122 2016
Size: 106 5035
ApaLI G'TGCA_C
Cuts at: 3911 3911
Size: 5141
ApoI r'AATT_y
Cuts at: 1690 2145 4494 4750 1690
Size: 455 2349 256 2081
Fragments arranged by size:
2349 2081 455 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5141
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5141
AvaI C'yCGr_G
Cuts at: 448 1152 2161 448
Size: 704 1009 3428
Fragments arranged by size:
3428 1009 704
BamHI G'GATC_C
Cuts at: 2166 2166
Size: 5141
BanI G'GyrC_C
Cuts at: 172 277 844 1062 1873 2157 2571 2819 2854
Size: 105 567 218 811 284 414 248 35
Cuts at: 2854 172
Size: 2459
Fragments arranged by size:
2459 811 567 414 284 248 218 105
35
BanII G_rGCy'C
Cuts at: 757 865 1513 2016 2063 2122 2155 3185 757
Size: 108 648 503 47 59 33 1030 2713
Fragments arranged by size:
2713 1030 648 503 108 59 47 33
BbeI G_GCGC'C
Cuts at: 176 281 1877 2575 2823 176
Size: 105 1596 698 248 2494
Fragments arranged by size:
2494 1596 698 248 105
BbsI GAAGACnn'nnnn_
Cuts at: 486 864 1090 1901 486
Size: 378 226 811 3726
Fragments arranged by size:
3726 811 378 226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 532 566 1851 1885 532
Size: 34 1285 34 3788
Fragments arranged by size:
3788 1285 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3039 3800 5141 3039
Size: 761 1341 3039
Fragments arranged by size:
3039 1341 761
BclI T'GATC_A
Cuts at: 2661 2661
Size: 5141
BglI GCCn_nnn'nGGC
Cuts at: 170 245 559 781 842 170
Size: 75 314 222 61 4469
Fragments arranged by size:
4469 314 222 75 61
BglII A'GATC_T
Cuts at: 1286 2656 4693 1286
Size: 1370 2037 1734
Fragments arranged by size:
2037 1734 1370
BlpI GC'TnA_GC
Cuts at: 2253 2253
Size: 5141
Bme1580I G_kGCm'C
Cuts at: 211 1067 2016 2111 2122 2766 2859 3915 211
Size: 856 949 95 11 644 93 1056 1437
Fragments arranged by size:
1437 1056 949 856 644 95 93 11
BmgBI CAC'GTC
Cuts at: 423 779 828 1073 423
Size: 356 49 245 4491
Fragments arranged by size:
4491 356 245 49
BmrI ACTGGGnnnn_n'
Cuts at: 322 2353 2434 2769 4913 322
Size: 2031 81 335 2144 550
Fragments arranged by size:
2144 2031 550 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1334 1758 3508 1334
Size: 424 1750 2967
Fragments arranged by size:
2967 1750 424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 870 1427 241
Size: 629 557 3955
Fragments arranged by size:
3955 629 557
Bpu10I CC'TnA_GC
Cuts at: 2057 2057
Size: 5141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2317 3196 3688 3986 2317
Size: 879 492 298 3472
Fragments arranged by size:
3472 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4845 4845
Size: 5141
BsaAI yAC'GTr
Cuts at: 621 660 1439 2494 621
Size: 39 779 1055 3268
Fragments arranged by size:
3268 1055 779 39
BsaBI GATnn'nnATC
Cuts at: 6 586 1291 2655 4692 6
Size: 580 705 1364 2037 455
Fragments arranged by size:
2037 1364 705 580 455
BsaHI Gr'CG_yC
Cuts at: 173 278 1727 1874 2572 2820 3522 5018 173
Size: 105 1449 147 698 248 702 1496 296
Fragments arranged by size:
1496 1449 702 698 296 248 147 105
BsaWI w'CCGG_w
Cuts at: 1295 2330 2851 3529 3803 3950 4361 1295
Size: 1035 521 678 274 147 411 2075
Fragments arranged by size:
2075 1035 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1255 1285 1255
Size: 30 5111
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 111 924 1263 1803 111
Size: 813 339 540 3449
Fragments arranged by size:
3449 813 540 339
BseYI C'CCAG_C
Cuts at: 149 1134 1330 2024 2071 2566 3324 3901 4397
Size: 985 196 694 47 495 758 577 496
Cuts at: 4397 149
Size: 893
Fragments arranged by size:
985 893 758 694 577 496 495 196
47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 1192
Size: 5141
BsiEI CG_ry'CG
Cuts at: 104 311 355 834 1871 2683 2729 3937 104
Size: 207 44 479 1037 812 46 1208 1308
Fragments arranged by size:
1308 1208 1037 812 479 207 46 44
BsiHKAI G_wGCw'C
Cuts at: 2063 2155 2933 3123 3915 2063
Size: 92 778 190 792 3289
Fragments arranged by size:
3289 792 778 190 92
BsmBI CGTCTCn'nnnn_
Cuts at: 384 1839 384
Size: 1455 3686
Bsp1286I G_dGCh'C
Cuts at: 211 757 865 1067 1513 2016 2063 2111 2122
Size: 546 108 202 446 503 47 48 11
Cuts at: 2122 2155 2766 2859 2933 3123 3185 3915 211
Size: 33 611 93 74 190 62 730 1437
Fragments arranged by size:
1437 730 611 546 503 446 202 190
108 93 74 62 48 47 33 11
BspEI T'CCGG_A
Cuts at: 1295 4361 1295
Size: 3066 2075
BspHI T'CATG_A
Cuts at: 1544 4317 1544
Size: 2773 2368
BspMI ACCTGCnnnn'nnnn_
Cuts at: 756 2191 2405 2707 3088 756
Size: 1435 214 302 381 2809
Fragments arranged by size:
2809 1435 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1458 3433 3487 4971 5136 50
Size: 1408 1975 54 1484 165 55
Fragments arranged by size:
1975 1484 1408 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3053 3053
Size: 5141
BsrFI r'CCGG_y
Cuts at: 357 460 640 1202 3139 3320 3529 357
Size: 103 180 562 1937 181 209 1969
Fragments arranged by size:
1969 1937 562 209 181 180 103
BsrGI T'GTAC_A
Cuts at: 905 905
Size: 5141
BssHII G'CGCG_C
Cuts at: 251 412 251
Size: 161 4980
BssSI C'ACGA_G
Cuts at: 3412 3770 3412
Size: 358 4783
BstAPI GCAn_nnn'nTGC
Cuts at: 2518 2518
Size: 5141
BstBI TT'CG_AA
Cuts at: 3502 3502
Size: 5141
BstXI CCAn_nnnn'nTGG
Cuts at: 1807 1807
Size: 5141
BstYI r'GATC_y
Cuts at: 1 809 1208 1286 2166 2633 2656 2991 3237
Size: 808 399 78 880 467 23 335 246
Cuts at: 3237 4249 4335 4347 4693 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 880 808 467 449 399 346 335
246 86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 814 814
Size: 5141
BtgI C'CryG_G
Cuts at: 107 113 349 563 734 866 107
Size: 6 236 214 171 132 4382
Fragments arranged by size:
4382 236 214 171 132 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 252 1074 3091 3272 3435 252
Size: 822 2017 181 163 1958
Fragments arranged by size:
2017 1958 822 181 163
BtsI GCAGTG_nn'
Cuts at: 1516 2043 2384 1516
Size: 527 341 4273
Fragments arranged by size:
4273 527 341
DraI TTT'AAA
Cuts at: 2140 2140
Size: 5141
DrdI GACnn_nn'nnGTC
Cuts at: 376 2847 3705 376
Size: 2471 858 1812
Fragments arranged by size:
2471 1812 858
EaeI y'GGCC_r
Cuts at: 110 197 308 352 560 782 1679 2726 2900
Size: 87 111 44 208 222 897 1047 174
Cuts at: 2900 3291 3318 4663 110
Size: 391 27 1345 588
Fragments arranged by size:
1345 1047 897 588 391 222 208 174
111 87 44 27
EagI C'GGCC_G
Cuts at: 308 352 2726 308
Size: 44 2374 2723
Fragments arranged by size:
2723 2374 44
EarI CTCTTCn'nnn_
Cuts at: 580 1448 1501 2068 3164 3374 580
Size: 868 53 567 1096 210 2347
Fragments arranged by size:
2347 1096 868 567 210 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 244 616 627 3349 3659 3805 4951 244
Size: 372 11 2722 310 146 1146 434
Fragments arranged by size:
2722 1146 434 372 310 146 11
EcoICRI GAG'CTC
Cuts at: 2061 2153 2061
Size: 92 5049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 507 870 1186 1427 2966 3398 4145 4413
Size: 266 363 316 241 1539 432 747 268
Cuts at: 4413 241
Size: 969
Fragments arranged by size:
1539 969 747 432 363 316 268 266
241
EcoO109I rG'GnC_Cy
Cuts at: 286 1302 1569 2012 2118 2119 2280 286
Size: 1016 267 443 106 1 161 3147
Fragments arranged by size:
3147 1016 443 267 161 106 1
EcoRI G'AATT_C
Cuts at: 2145 2145
Size: 5141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 228 3160 228
Size: 2932 2209
FspI TGC'GCA
Cuts at: 1771 2922 1771
Size: 1151 3990
FspAI rTGC'GCAy
Cuts at: 1771 1771
Size: 5141
HaeII r_GCGC'y
Cuts at: 150 176 281 1877 2575 2823 3845 4824 150
Size: 26 105 1596 698 248 1022 979 467
Fragments arranged by size:
1596 1022 979 698 467 248 105 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1459 1491 1836 1868 2116 2148 2983 3013 3015
Size: 32 345 32 248 32 835 30 2
Cuts at: 3015 3045 1459
Size: 30 3555
Fragments arranged by size:
3555 835 345 248 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 831 1482 2052 2180 831
Size: 651 570 128 3792
Fragments arranged by size:
3792 651 570 128
KasI G'GCGC_C
Cuts at: 172 277 1873 2571 2819 172
Size: 105 1596 698 248 2494
Fragments arranged by size:
2494 1596 698 248 105
KpnI G_GTAC'C
Cuts at: 2161 2161
Size: 5141
MluI A'CGCG_T
Cuts at: 4574 4574
Size: 5141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 362 3812 3996 362
Size: 3450 184 1507
Fragments arranged by size:
3450 1507 184
MscI TGG'CCA
Cuts at: 199 562 1681 2902 199
Size: 363 1119 1221 2438
Fragments arranged by size:
2438 1221 1119 363
MslI CAynn'nnrTG
Cuts at: 1245 1939 3130 3257 1245
Size: 694 1191 127 3129
Fragments arranged by size:
3129 1191 694 127
MspA1I CmG'CkG
Cuts at: 115 351 1138 1623 1911 1953 1988 2100 2252
Size: 236 787 485 288 42 35 112 152
Cuts at: 2252 2566 2926 3939 4184 115
Size: 314 360 1013 245 1072
Fragments arranged by size:
1072 1013 787 485 360 314 288 245
236 152 112 42 35
NaeI GCC'GGC
Cuts at: 642 3322 642
Size: 2680 2461
NarI GG'CG_CC
Cuts at: 173 278 1874 2572 2820 173
Size: 105 1596 698 248 2494
Fragments arranged by size:
2494 1596 698 248 105
NcoI C'CATG_G
Cuts at: 107 734 107
Size: 627 4514
NgoMIV G'CCGG_C
Cuts at: 640 3320 640
Size: 2680 2461
NotI GC'GGCC_GC
Cuts at: 352 352
Size: 5141
NspI r_CATG'y
Cuts at: 1536 1668 1944 2194 2694 3601 1536
Size: 132 276 250 500 907 3076
Fragments arranged by size:
3076 907 500 276 250 132
PasI CC'CwG_GG
Cuts at: 131 131
Size: 5141
PciI A'CATG_T
Cuts at: 3597 3597
Size: 5141
PflMI CCAn_nnn'nTGG
Cuts at: 464 1923 464
Size: 1459 3682
PfoI T'CCnGG_A
Cuts at: 1404 1404
Size: 5141
PmeI GTTT'AAAC
Cuts at: 2140 2140
Size: 5141
PmlI CAC'GTG
Cuts at: 1439 1439
Size: 5141
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1255 1287 4280 4312 1255
Size: 32 2993 32 2084
Fragments arranged by size:
2993 2084 32 32
PpuMI rG'GwC_Cy
Cuts at: 286 1569 286
Size: 1283 3858
PshAI GACnn'nnGTC
Cuts at: 1075 1075
Size: 5141
PspOMI G'GGCC_C
Cuts at: 2012 2118 2012
Size: 106 5035
PspXI vC'TCGA_Gb
Cuts at: 448 448
Size: 5141
PstI C_TGCA'G
Cuts at: 809 1787 2188 2873 809
Size: 978 401 685 3077
Fragments arranged by size:
3077 978 685 401
PvuI CG_AT'CG
Cuts at: 104 1871 104
Size: 1767 3374
PvuII CAG'CTG
Cuts at: 1623 1911 1953 2100 2566 2926 1623
Size: 288 42 147 466 360 3838
Fragments arranged by size:
3838 466 360 288 147 42
RsrII CG'GwC_CG
Cuts at: 3336 3336
Size: 5141
SacI G_AGCT'C
Cuts at: 2063 2155 2063
Size: 92 5049
SacII CC_GC'GG
Cuts at: 116 352 116
Size: 236 4905
SalI G'TCGA_C
Cuts at: 829 1480 2050 2178 829
Size: 651 570 128 3792
Fragments arranged by size:
3792 651 570 128
SapI GCTCTTCn'nnn_
Cuts at: 1448 1501 2068 3164 3374 1448
Size: 53 567 1096 210 3215
Fragments arranged by size:
3215 1096 567 210 53
SbfI CC_TGCA'GG
Cuts at: 2188 2188
Size: 5141
ScaI AGT'ACT
Cuts at: 1672 1741 4658 1672
Size: 69 2917 2155
Fragments arranged by size:
2917 2155 69
SfcI C'TryA_G
Cuts at: 33 387 805 1783 2184 2869 3862 4053 33
Size: 354 418 978 401 685 993 191 1121
Fragments arranged by size:
1121 993 978 685 418 401 354 191
SfoI GGC'GCC
Cuts at: 174 279 1875 2573 2821 174
Size: 105 1596 698 248 2494
Fragments arranged by size:
2494 1596 698 248 105
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5141
SgrAI Cr'CCGG_yG
Cuts at: 357 357
Size: 5141
SmaI CCC'GGG
Cuts at: 2163 2163
Size: 5141
SmlI C'TyrA_G
Cuts at: 448 2296 3211 3703 3965 448
Size: 1848 915 492 262 1624
Fragments arranged by size:
1848 1624 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5141
SphI G_CATG'C
Cuts at: 1536 1668 2194 2694 1536
Size: 132 526 500 3983
Fragments arranged by size:
3983 526 500 132
StyI C'CwwG_G
Cuts at: 107 342 734 758 2275 2629 107
Size: 235 392 24 1517 354 2619
Fragments arranged by size:
2619 1517 392 354 235 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 433 1344 1617 1624 2038 2724 2857 3316 3525
Size: 911 273 7 414 686 133 459 209
Cuts at: 3525 433
Size: 2049
Fragments arranged by size:
2049 911 686 459 414 273 209 133
7
TatI w'GTAC_w
Cuts at: 905 1670 1739 4656 905
Size: 765 69 2917 1390
Fragments arranged by size:
2917 1390 765 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4658 4690 4658
Size: 32 5109
Tth111I GACn'n_nGTC
Cuts at: 2938 2938
Size: 5141
XbaI T'CTAG_A
Cuts at: 2172 2172
Size: 5141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1350 2636 1350
Size: 1286 3855
XhoI C'TCGA_G
Cuts at: 448 448
Size: 5141
XmaI C'CCGG_G
Cuts at: 2161 2161
Size: 5141
XmnI GAAnn'nnTTC
Cuts at: 472 1195 1690 472
Size: 723 495 3923
Fragments arranged by size:
3923 723 495
ZraI GAC'GTC
Cuts at: 1728 1728
Size: 5141
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AleI AscI AvrII BaeI BbvCI BfrBI
BmtI BsiWI BsmI BstEII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PsiI PsrI SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI