Restriction map for
pF1KB7192
(Circular) (Six-base) MAPSORT of: pF1KB7192.seq Check: 1485 from: 1 to: 4064
pF1KB7192 4064 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 478 1102 478
Size: 624 3440
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 1889 2321 3068 3336 221
Size: 1668 432 747 268 949
Fragments arranged by size:
1668 949 747 432 268
AflIII A'CryG_T
Cuts at: 1416 2520 3497 1416
Size: 1104 977 1983
Fragments arranged by size:
1983 1104 977
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 1369 2936 1369
Size: 1567 2497
ApaLI G'TGCA_C
Cuts at: 2834 2834
Size: 4064
ApoI r'AATT_y
Cuts at: 168 1068 3417 3673 168
Size: 900 2349 256 559
Fragments arranged by size:
2349 900 559 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 1084 1084
Size: 4064
BamHI G'GATC_C
Cuts at: 1089 1089
Size: 4064
BanI G'GyrC_C
Cuts at: 640 775 907 1080 1494 1742 1777 640
Size: 135 132 173 414 248 35 2927
Fragments arranged by size:
2927 414 248 173 135 132 35
BanII G_rGCy'C
Cuts at: 1078 2108 1078
Size: 1030 3034
BbeI G_GCGC'C
Cuts at: 911 1498 1746 911
Size: 587 248 3229
Fragments arranged by size:
3229 587 248
BbsI GAAGACnn'nnnn_
Cuts at: 118 407 749 790 118
Size: 289 342 41 3392
Fragments arranged by size:
3392 342 289 41
BciVI GTATCCnnnnn_n'
Cuts at: 1962 2723 4064 1962
Size: 761 1341 1962
Fragments arranged by size:
1962 1341 761
BclI T'GATC_A
Cuts at: 1584 1584
Size: 4064
BglII A'GATC_T
Cuts at: 1579 3616 1579
Size: 2037 2027
BlpI GC'TnA_GC
Cuts at: 130 1176 130
Size: 1046 3018
Bme1580I G_kGCm'C
Cuts at: 778 1689 1782 2838 778
Size: 911 93 1056 2004
Fragments arranged by size:
2004 1056 911 93
BmrI ACTGGGnnnn_n'
Cuts at: 451 1276 1357 1692 3836 451
Size: 825 81 335 2144 679
Fragments arranged by size:
2144 825 679 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 4064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 749
Size: 4064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2119 2611 2909 1240
Size: 879 492 298 2395
Fragments arranged by size:
2395 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 748 3768 748
Size: 3020 1044
BsaAI yAC'GTr
Cuts at: 1417 1417
Size: 4064
BsaBI GATnn'nnATC
Cuts at: 6 1578 3615 6
Size: 1572 2037 455
Fragments arranged by size:
2037 1572 455
BsaHI Gr'CG_yC
Cuts at: 458 908 1495 1743 2445 3941 458
Size: 450 587 248 702 1496 581
Fragments arranged by size:
1496 702 587 581 450 248
BsaWI w'CCGG_w
Cuts at: 757 1253 1774 2452 2726 2873 3284 757
Size: 496 521 678 274 147 411 1537
Fragments arranged by size:
1537 678 521 496 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 780 810 780
Size: 30 4034
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 355 367 583 704 352
Size: 3 12 216 121 3712
Fragments arranged by size:
3712 216 121 12 3
BseYI C'CCAG_C
Cuts at: 514 1489 2247 2824 3320 514
Size: 975 758 577 496 1258
Fragments arranged by size:
1258 975 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1606 1652 2860 104
Size: 1502 46 1208 1308
Fragments arranged by size:
1502 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1078 1856 2046 2838 1078
Size: 778 190 792 2304
Fragments arranged by size:
2304 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 778 1078 1689 1782 1856 2046 2108 2838 778
Size: 300 611 93 74 190 62 730 2004
Fragments arranged by size:
2004 730 611 300 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3284 3284
Size: 4064
BspHI T'CATG_A
Cuts at: 3240 3240
Size: 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1114 1328 1630 2011 1114
Size: 214 302 381 3167
Fragments arranged by size:
3167 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2356 2410 3894 4059 50
Size: 2306 54 1484 165 55
Fragments arranged by size:
2306 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1976 1976
Size: 4064
BsrFI r'CCGG_y
Cuts at: 2062 2243 2452 2062
Size: 181 209 3674
Fragments arranged by size:
3674 209 181
BssSI C'ACGA_G
Cuts at: 962 2335 2693 962
Size: 1373 358 2333
Fragments arranged by size:
2333 1373 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1441 1441
Size: 4064
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstYI r'GATC_y
Cuts at: 1 1089 1556 1579 1914 2160 3172 3258 3270
Size: 1088 467 23 335 246 1012 86 12
Cuts at: 3270 3616 1
Size: 346 449
Fragments arranged by size:
1088 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 479 479
Size: 4064
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2014 2195 2358 2014
Size: 181 163 3720
Fragments arranged by size:
3720 181 163
BtsI GCAGTG_nn'
Cuts at: 1307 1307
Size: 4064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 858 893 858
Size: 35 4029
DraI TTT'AAA
Cuts at: 272 1008 1063 272
Size: 736 55 3273
Fragments arranged by size:
3273 736 55
DrdI GACnn_nn'nnGTC
Cuts at: 1770 2628 1770
Size: 858 3206
EaeI y'GGCC_r
Cuts at: 1649 1823 2214 2241 3586 1649
Size: 174 391 27 1345 2127
Fragments arranged by size:
2127 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1649 1649
Size: 4064
EarI CTCTTCn'nnn_
Cuts at: 174 329 344 560 569 2087 2297 174
Size: 155 15 216 9 1518 210 1941
Fragments arranged by size:
1941 1518 216 210 155 15 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2272 2582 2728 3874 2272
Size: 310 146 1146 2462
Fragments arranged by size:
2462 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1076 1076
Size: 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 749 1889 2321 3068 3336 221
Size: 528 1140 432 747 268 949
Fragments arranged by size:
1140 949 747 528 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 470 470
Size: 4064
EcoO109I rG'GnC_Cy
Cuts at: 1203 1203
Size: 4064
EcoRI G'AATT_C
Cuts at: 1068 1068
Size: 4064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 4064
FspI TGC'GCA
Cuts at: 1845 1845
Size: 4064
HaeII r_GCGC'y
Cuts at: 911 1498 1746 2768 3747 911
Size: 587 248 1022 979 1228
Fragments arranged by size:
1228 1022 979 587 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 388 420 920 952 1906 1936 1938 1968 388
Size: 32 500 32 954 30 2 30 2484
Fragments arranged by size:
2484 954 500 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1103 1103
Size: 4064
KasI G'GCGC_C
Cuts at: 907 1494 1742 907
Size: 587 248 3229
Fragments arranged by size:
3229 587 248
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2735 2919 2735
Size: 184 3880
MscI TGG'CCA
Cuts at: 1825 1825
Size: 4064
MslI CAynn'nnrTG
Cuts at: 2053 2180 2053
Size: 127 3937
MspA1I CmG'CkG
Cuts at: 686 1175 1489 1849 2862 3107 686
Size: 489 314 360 1013 245 1643
Fragments arranged by size:
1643 1013 489 360 314 245
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 4064
NarI GG'CG_CC
Cuts at: 908 1495 1743 908
Size: 587 248 3229
Fragments arranged by size:
3229 587 248
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 4064
NspI r_CATG'y
Cuts at: 253 1117 1617 2524 253
Size: 864 500 907 1793
Fragments arranged by size:
1793 907 864 500
PciI A'CATG_T
Cuts at: 2520 2520
Size: 4064
PflMI CCAn_nnn'nTGG
Cuts at: 805 859 805
Size: 54 4010
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 4032
PstI C_TGCA'G
Cuts at: 1111 1796 1111
Size: 685 3379
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 1489 1849 1489
Size: 360 3704
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 1078 1078
Size: 4064
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SexAI A'CCwGG_T
Cuts at: 1055 1055
Size: 4064
SfcI C'TryA_G
Cuts at: 33 837 1107 1792 2785 2976 33
Size: 804 270 685 993 191 1121
Fragments arranged by size:
1121 993 804 685 270 191
SfoI GGC'GCC
Cuts at: 909 1496 1744 909
Size: 587 248 3229
Fragments arranged by size:
3229 587 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 1219 2134 2626 2888 1219
Size: 915 492 262 2395
Fragments arranged by size:
2395 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 253 1117 1617 253
Size: 864 500 2700
Fragments arranged by size:
2700 864 500
StyI C'CwwG_G
Cuts at: 1198 1552 1198
Size: 354 3710
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1647 1780 2239 2448 1647
Size: 133 459 209 3263
Fragments arranged by size:
3263 459 209 133
TatI w'GTAC_w
Cuts at: 221 995 3579 221
Size: 774 2584 706
Fragments arranged by size:
2584 774 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4032
Tth111I GACn'n_nGTC
Cuts at: 1861 1861
Size: 4064
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1559 1559
Size: 4064
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
XmnI GAAnn'nnTTC
Cuts at: 846 846
Size: 4064
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI Bsu36I BtgI ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI