Restriction map for
pF1KB7191
(Circular) (Six-base) MAPSORT of: pF1KB7191.seq Check: 1518 from: 1 to: 4736
pF1KB7191 4736 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1774 1774
Size: 4736
Acc65I G'GTAC_C
Cuts at: 1752 1752
Size: 4736
AclI AA'CG_TT
Cuts at: 458 4576 458
Size: 4118 618
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2561 2993 3740 4008 2561
Size: 432 747 268 3289
Fragments arranged by size:
3289 747 432 268
AfeI AGC'GCT
Cuts at: 1080 1080
Size: 4736
AflII C'TTAA_G
Cuts at: 1170 1170
Size: 4736
AflIII A'CryG_T
Cuts at: 2088 3192 4169 2088
Size: 1104 977 2655
Fragments arranged by size:
2655 1104 977
AgeI A'CCGG_T
Cuts at: 3124 3124
Size: 4736
AhdI GACnn_n'nnGTC
Cuts at: 669 669
Size: 4736
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1120 1120
Size: 4736
AlwNI CAG_nnn'CTG
Cuts at: 406 754 2041 3608 406
Size: 348 1287 1567 1534
Fragments arranged by size:
1567 1534 1287 348
ApaLI G'TGCA_C
Cuts at: 3506 3506
Size: 4736
ApoI r'AATT_y
Cuts at: 323 513 1740 4089 4345 323
Size: 190 1227 2349 256 714
Fragments arranged by size:
2349 1227 714 256 190
AseI AT'TA_AT
Cuts at: 20 802 20
Size: 782 3954
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4736
AvaI C'yCGr_G
Cuts at: 1756 1756
Size: 4736
BamHI G'GATC_C
Cuts at: 1761 1761
Size: 4736
BanI G'GyrC_C
Cuts at: 1752 2166 2414 2449 1752
Size: 414 248 35 4039
Fragments arranged by size:
4039 414 248 35
BanII G_rGCy'C
Cuts at: 1675 1750 2780 1675
Size: 75 1030 3631
Fragments arranged by size:
3631 1030 75
BbeI G_GCGC'C
Cuts at: 2170 2418 2170
Size: 248 4488
BbsI GAAGACnn'nnnn_
Cuts at: 624 624
Size: 4736
BbvCI CC'TCA_GC
Cuts at: 1657 1657
Size: 4736
BciVI GTATCCnnnnn_n'
Cuts at: 2634 3395 4736 2634
Size: 761 1341 2634
Fragments arranged by size:
2634 1341 761
BclI T'GATC_A
Cuts at: 2256 2256
Size: 4736
BfrBI ATG'CAT
Cuts at: 840 840
Size: 4736
BglII A'GATC_T
Cuts at: 358 1664 2251 4288 358
Size: 1306 587 2037 806
Fragments arranged by size:
2037 1306 806 587
BlpI GC'TnA_GC
Cuts at: 1674 1848 1674
Size: 174 4562
Bme1580I G_kGCm'C
Cuts at: 2361 2454 3510 2361
Size: 93 1056 3587
Fragments arranged by size:
3587 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1948 2029 2364 4508 1948
Size: 81 335 2144 2176
Fragments arranged by size:
2176 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1575 3103 1575
Size: 1528 3208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 1689 383
Size: 1306 3430
Bpu10I CC'TnA_GC
Cuts at: 1657 1657
Size: 4736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1912 2791 3283 3581 607
Size: 1305 879 492 298 1762
Fragments arranged by size:
1762 1305 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 520 1500 4440 520
Size: 980 2940 816
Fragments arranged by size:
2940 980 816
BsaAI yAC'GTr
Cuts at: 2089 2089
Size: 4736
BsaBI GATnn'nnATC
Cuts at: 6 2250 4287 6
Size: 2244 2037 455
Fragments arranged by size:
2244 2037 455
BsaHI Gr'CG_yC
Cuts at: 2167 2415 3117 4613 2167
Size: 248 702 1496 2290
Fragments arranged by size:
2290 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1925 2446 3124 3398 3545 3956 1925
Size: 521 678 274 147 411 2705
Fragments arranged by size:
2705 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 646 646
Size: 4736
BseYI C'CCAG_C
Cuts at: 1450 2161 2919 3496 3992 1450
Size: 711 758 577 496 2194
Fragments arranged by size:
2194 758 711 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 1136 704
Size: 432 4304
BsiEI CG_ry'CG
Cuts at: 104 2278 2324 3532 104
Size: 2174 46 1208 1308
Fragments arranged by size:
2174 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1675 1750 2528 2718 3510 1675
Size: 75 778 190 792 2901
Fragments arranged by size:
2901 792 778 190 75
Bsp1286I G_dGCh'C
Cuts at: 1675 1750 2361 2454 2528 2718 2780 3510 1675
Size: 75 611 93 74 190 62 730 2901
Fragments arranged by size:
2901 730 611 190 93 75 74 62
BspEI T'CCGG_A
Cuts at: 3956 3956
Size: 4736
BspHI T'CATG_A
Cuts at: 239 3912 239
Size: 3673 1063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1786 2000 2302 2683 1786
Size: 214 302 381 3839
Fragments arranged by size:
3839 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3028 3082 4566 4731 50
Size: 2978 54 1484 165 55
Fragments arranged by size:
2978 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2648 2648
Size: 4736
BsrFI r'CCGG_y
Cuts at: 2734 2915 3124 2734
Size: 181 209 4346
Fragments arranged by size:
4346 209 181
BsrGI T'GTAC_A
Cuts at: 258 258
Size: 4736
BssSI C'ACGA_G
Cuts at: 1102 1321 3007 3365 1102
Size: 219 1686 358 2473
Fragments arranged by size:
2473 1686 358 219
BstAPI GCAn_nnn'nTGC
Cuts at: 2113 2113
Size: 4736
BstBI TT'CG_AA
Cuts at: 3097 3097
Size: 4736
BstXI CCAn_nnnn'nTGG
Cuts at: 1416 1491 1416
Size: 75 4661
BstYI r'GATC_y
Cuts at: 1 358 1664 1761 2228 2251 2586 2832 3844
Size: 357 1306 97 467 23 335 246 1012
Cuts at: 3844 3930 3942 4288 1
Size: 86 12 346 449
Fragments arranged by size:
1306 1012 467 449 357 346 335 246
97 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4736
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2686 2867 3030 2686
Size: 181 163 4392
Fragments arranged by size:
4392 181 163
BtsI GCAGTG_nn'
Cuts at: 1979 1979
Size: 4736
DraI TTT'AAA
Cuts at: 1248 1735 1248
Size: 487 4249
DrdI GACnn_nn'nnGTC
Cuts at: 628 2442 3300 628
Size: 1814 858 2064
Fragments arranged by size:
2064 1814 858
EaeI y'GGCC_r
Cuts at: 764 2321 2495 2886 2913 4258 764
Size: 1557 174 391 27 1345 1242
Fragments arranged by size:
1557 1345 1242 391 174 27
EagI C'GGCC_G
Cuts at: 2321 2321
Size: 4736
EarI CTCTTCn'nnn_
Cuts at: 2759 2969 2759
Size: 210 4526
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2944 3254 3400 4546 2944
Size: 310 146 1146 3134
Fragments arranged by size:
3134 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1673 1748 1673
Size: 75 4661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 1689 2561 2993 3740 4008 383
Size: 1306 872 432 747 268 1111
Fragments arranged by size:
1306 1111 872 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 663 1593 1875 663
Size: 930 282 3524
Fragments arranged by size:
3524 930 282
EcoRI G'AATT_C
Cuts at: 1740 1740
Size: 4736
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 368 939 2755 368
Size: 571 1816 2349
Fragments arranged by size:
2349 1816 571
FspI TGC'GCA
Cuts at: 2517 2517
Size: 4736
HaeII r_GCGC'y
Cuts at: 1082 2170 2418 3440 4419 1082
Size: 1088 248 1022 979 1399
Fragments arranged by size:
1399 1088 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 655 687 2578 2608 2610 2640 655
Size: 32 1891 30 2 30 2751
Fragments arranged by size:
2751 1891 32 30 30 2
HincII GTy'rAC
Cuts at: 144 1775 144
Size: 1631 3105
KasI G'GCGC_C
Cuts at: 2166 2414 2166
Size: 248 4488
KpnI G_GTAC'C
Cuts at: 1756 1756
Size: 4736
MfeI C'AATT_G
Cuts at: 1571 1571
Size: 4736
MluI A'CGCG_T
Cuts at: 4169 4169
Size: 4736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 512 1598 1604 3407 3591 512
Size: 1086 6 1803 184 1657
Fragments arranged by size:
1803 1657 1086 184 6
MscI TGG'CCA
Cuts at: 766 2497 766
Size: 1731 3005
MslI CAynn'nnrTG
Cuts at: 680 970 2725 2852 680
Size: 290 1755 127 2564
Fragments arranged by size:
2564 1755 290 127
MspA1I CmG'CkG
Cuts at: 1120 1847 2161 2521 3534 3779 1120
Size: 727 314 360 1013 245 2077
Fragments arranged by size:
2077 1013 727 360 314 245
NaeI GCC'GGC
Cuts at: 2917 2917
Size: 4736
NarI GG'CG_CC
Cuts at: 2167 2415 2167
Size: 248 4488
NcoI C'CATG_G
Cuts at: 107 107
Size: 4736
NgoMIV G'CCGG_C
Cuts at: 2915 2915
Size: 4736
NsiI A_TGCA'T
Cuts at: 842 842
Size: 4736
NspI r_CATG'y
Cuts at: 840 1481 1789 2289 3196 840
Size: 641 308 500 907 2380
Fragments arranged by size:
2380 907 641 500 308
PciI A'CATG_T
Cuts at: 3192 3192
Size: 4736
PfoI T'CCnGG_A
Cuts at: 659 659
Size: 4736
PmeI GTTT'AAAC
Cuts at: 1735 1735
Size: 4736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 3875
Size: 32 4704
PpuMI rG'GwC_Cy
Cuts at: 663 663
Size: 4736
PstI C_TGCA'G
Cuts at: 1089 1783 2468 1089
Size: 694 685 3357
Fragments arranged by size:
3357 694 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4736
PvuII CAG'CTG
Cuts at: 1120 2161 2521 1120
Size: 1041 360 3335
Fragments arranged by size:
3335 1041 360
RsrII CG'GwC_CG
Cuts at: 2931 2931
Size: 4736
SacI G_AGCT'C
Cuts at: 1675 1750 1675
Size: 75 4661
SalI G'TCGA_C
Cuts at: 1773 1773
Size: 4736
SanDI GG'GwC_CC
Cuts at: 663 663
Size: 4736
SapI GCTCTTCn'nnn_
Cuts at: 2759 2969 2759
Size: 210 4526
SbfI CC_TGCA'GG
Cuts at: 1783 1783
Size: 4736
ScaI AGT'ACT
Cuts at: 4253 4253
Size: 4736
SfcI C'TryA_G
Cuts at: 33 1085 1779 2464 3457 3648 33
Size: 1052 694 685 993 191 1121
Fragments arranged by size:
1121 1052 993 694 685 191
SfoI GGC'GCC
Cuts at: 2168 2416 2168
Size: 248 4488
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4736
SmaI CCC'GGG
Cuts at: 1758 1758
Size: 4736
SmlI C'TyrA_G
Cuts at: 586 1170 1891 2806 3298 3560 586
Size: 584 721 915 492 262 1762
Fragments arranged by size:
1762 915 721 584 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4736
SphI G_CATG'C
Cuts at: 1481 1789 2289 1481
Size: 308 500 3928
Fragments arranged by size:
3928 500 308
SspI AAT'ATT
Cuts at: 879 1611 879
Size: 732 4004
StuI AGG'CCT
Cuts at: 1490 1490
Size: 4736
StyI C'CwwG_G
Cuts at: 107 1680 1870 2224 107
Size: 1573 190 354 2619
Fragments arranged by size:
2619 1573 354 190
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 737 1009 1540 2319 2452 2911 3120 737
Size: 272 531 779 133 459 209 2353
Fragments arranged by size:
2353 779 531 459 272 209 133
TatI w'GTAC_w
Cuts at: 258 1575 4251 258
Size: 1317 2676 743
Fragments arranged by size:
2676 1317 743
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 406 438 4253 4285 406
Size: 32 3815 32 857
Fragments arranged by size:
3815 857 32 32
Tth111I GACn'n_nGTC
Cuts at: 1376 2533 1376
Size: 1157 3579
XbaI T'CTAG_A
Cuts at: 1767 1767
Size: 4736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2231 2231
Size: 4736
XmaI C'CCGG_G
Cuts at: 1756 1756
Size: 4736
XmnI GAAnn'nnTTC
Cuts at: 327 351 327
Size: 24 4712
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AleI AloI ApaI AscI AvrII BaeI
BcgI BglI BmgBI BmtI BsaXI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI