Restriction map for
pF1KB7189
(Circular) (Six-base) MAPSORT of: pF1KB7189.seq Check: 3031 from: 1 to: 3506
pF1KB7189 3506 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 544 544
Size: 3506
Acc65I G'GTAC_C
Cuts at: 522 522
Size: 3506
AclI AA'CG_TT
Cuts at: 3346 3346
Size: 3506
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1331 1763 2510 2778 1331
Size: 432 747 268 2059
Fragments arranged by size:
2059 747 432 268
AflIII A'CryG_T
Cuts at: 858 1962 2939 858
Size: 1104 977 1425
Fragments arranged by size:
1425 1104 977
AgeI A'CCGG_T
Cuts at: 1894 1894
Size: 3506
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 103
Size: 32 3474
AlwNI CAG_nnn'CTG
Cuts at: 213 811 2378 213
Size: 598 1567 1341
Fragments arranged by size:
1567 1341 598
ApaI G_GGCC'C
Cuts at: 326 326
Size: 3506
ApaLI G'TGCA_C
Cuts at: 2276 2276
Size: 3506
ApoI r'AATT_y
Cuts at: 510 2859 3115 510
Size: 2349 256 901
Fragments arranged by size:
2349 901 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3506
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3506
AvaI C'yCGr_G
Cuts at: 327 439 526 327
Size: 112 87 3307
Fragments arranged by size:
3307 112 87
BamHI G'GATC_C
Cuts at: 531 531
Size: 3506
BanI G'GyrC_C
Cuts at: 443 522 936 1184 1219 443
Size: 79 414 248 35 2730
Fragments arranged by size:
2730 414 248 79 35
BanII G_rGCy'C
Cuts at: 326 520 1550 326
Size: 194 1030 2282
Fragments arranged by size:
2282 1030 194
BbeI G_GCGC'C
Cuts at: 447 940 1188 447
Size: 493 248 2765
Fragments arranged by size:
2765 493 248
BciVI GTATCCnnnnn_n'
Cuts at: 1404 2165 3506 1404
Size: 761 1341 1404
Fragments arranged by size:
1404 1341 761
BclI T'GATC_A
Cuts at: 1026 1026
Size: 3506
BglI GCCn_nnn'nGGC
Cuts at: 410 410
Size: 3506
BglII A'GATC_T
Cuts at: 1021 3058 1021
Size: 2037 1469
BlpI GC'TnA_GC
Cuts at: 618 618
Size: 3506
Bme1580I G_kGCm'C
Cuts at: 326 1131 1224 2280 326
Size: 805 93 1056 1552
Fragments arranged by size:
1552 1056 805 93
BmrI ACTGGGnnnn_n'
Cuts at: 718 799 1134 3278 718
Size: 81 335 2144 946
Fragments arranged by size:
2144 946 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1873 1873
Size: 3506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 319
Size: 3506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1561 2053 2351 682
Size: 879 492 298 1837
Fragments arranged by size:
1837 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 135 3210 135
Size: 3075 431
BsaAI yAC'GTr
Cuts at: 859 859
Size: 3506
BsaBI GATnn'nnATC
Cuts at: 6 1020 3057 6
Size: 1014 2037 455
Fragments arranged by size:
2037 1014 455
BsaHI Gr'CG_yC
Cuts at: 444 937 1185 1887 3383 444
Size: 493 248 702 1496 567
Fragments arranged by size:
1496 702 567 493 248
BsaWI w'CCGG_w
Cuts at: 695 1216 1894 2168 2315 2726 695
Size: 521 678 274 147 411 1475
Fragments arranged by size:
1475 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 303 931 1689 2266 2762 303
Size: 628 758 577 496 1047
Fragments arranged by size:
1047 758 628 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 250 188
Size: 62 3444
BsiEI CG_ry'CG
Cuts at: 104 290 464 1048 1094 2302 104
Size: 186 174 584 46 1208 1308
Fragments arranged by size:
1308 1208 584 186 174 46
BsiHKAI G_wGCw'C
Cuts at: 520 1298 1488 2280 520
Size: 778 190 792 1746
Fragments arranged by size:
1746 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 326 520 1131 1224 1298 1488 1550 2280 326
Size: 194 611 93 74 190 62 730 1552
Fragments arranged by size:
1552 730 611 194 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2726 2726
Size: 3506
BspHI T'CATG_A
Cuts at: 2682 2682
Size: 3506
BspMI ACCTGCnnnn'nnnn_
Cuts at: 556 770 1072 1453 556
Size: 214 302 381 2609
Fragments arranged by size:
2609 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1798 1852 3336 3501 50
Size: 1748 54 1484 165 55
Fragments arranged by size:
1748 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1418 1418
Size: 3506
BsrFI r'CCGG_y
Cuts at: 289 1504 1685 1894 289
Size: 1215 181 209 1901
Fragments arranged by size:
1901 1215 209 181
BssSI C'ACGA_G
Cuts at: 1777 2135 1777
Size: 358 3148
BstAPI GCAn_nnn'nTGC
Cuts at: 883 883
Size: 3506
BstBI TT'CG_AA
Cuts at: 1867 1867
Size: 3506
BstYI r'GATC_y
Cuts at: 1 531 998 1021 1356 1602 2614 2700 2712
Size: 530 467 23 335 246 1012 86 12
Cuts at: 2712 3058 1
Size: 346 449
Fragments arranged by size:
1012 530 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 380 395 380
Size: 15 3491
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1456 1637 1800 1456
Size: 181 163 3162
Fragments arranged by size:
3162 181 163
BtsI GCAGTG_nn'
Cuts at: 749 749
Size: 3506
DraI TTT'AAA
Cuts at: 505 505
Size: 3506
DrdI GACnn_nn'nnGTC
Cuts at: 1212 2070 1212
Size: 858 2648
EaeI y'GGCC_r
Cuts at: 287 461 1091 1265 1656 1683 3028 287
Size: 174 630 174 391 27 1345 765
Fragments arranged by size:
1345 765 630 391 174 174 27
EagI C'GGCC_G
Cuts at: 287 461 1091 287
Size: 174 630 2702
Fragments arranged by size:
2702 630 174
EarI CTCTTCn'nnn_
Cuts at: 1529 1739 1529
Size: 210 3296
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 444 1714 2024 2170 3316 444
Size: 1270 310 146 1146 634
Fragments arranged by size:
1270 1146 634 310 146
EcoICRI GAG'CTC
Cuts at: 518 518
Size: 3506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 1331 1763 2510 2778 319
Size: 1012 432 747 268 1047
Fragments arranged by size:
1047 1012 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 358 358
Size: 3506
EcoO109I rG'GnC_Cy
Cuts at: 323 645 323
Size: 322 3184
EcoRI G'AATT_C
Cuts at: 510 510
Size: 3506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1525 1525
Size: 3506
FspI TGC'GCA
Cuts at: 1287 1287
Size: 3506
HaeII r_GCGC'y
Cuts at: 354 427 447 940 1188 2210 3189 354
Size: 73 20 493 248 1022 979 671
Fragments arranged by size:
1022 979 671 493 248 73 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1348 1378 1380 1410 1348
Size: 30 2 30 3444
Fragments arranged by size:
3444 30 30 2
HincII GTy'rAC
Cuts at: 545 545
Size: 3506
KasI G'GCGC_C
Cuts at: 443 936 1184 443
Size: 493 248 2765
Fragments arranged by size:
2765 493 248
KpnI G_GTAC'C
Cuts at: 526 526
Size: 3506
MluI A'CGCG_T
Cuts at: 2939 2939
Size: 3506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2177 2361 2177
Size: 184 3322
MscI TGG'CCA
Cuts at: 1267 1267
Size: 3506
MslI CAynn'nnrTG
Cuts at: 1495 1622 1495
Size: 127 3379
MspA1I CmG'CkG
Cuts at: 368 382 449 617 931 1291 2304 2549 368
Size: 14 67 168 314 360 1013 245 1325
Fragments arranged by size:
1325 1013 360 314 245 168 67 14
NaeI GCC'GGC
Cuts at: 291 1687 291
Size: 1396 2110
NarI GG'CG_CC
Cuts at: 444 937 1185 444
Size: 493 248 2765
Fragments arranged by size:
2765 493 248
NcoI C'CATG_G
Cuts at: 395 395
Size: 3506
NgoMIV G'CCGG_C
Cuts at: 289 1685 289
Size: 1396 2110
NspI r_CATG'y
Cuts at: 559 1059 1966 559
Size: 500 907 2099
Fragments arranged by size:
2099 907 500
PciI A'CATG_T
Cuts at: 1962 1962
Size: 3506
PfoI T'CCnGG_A
Cuts at: 493 493
Size: 3506
PmeI GTTT'AAAC
Cuts at: 505 505
Size: 3506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 3474
PshAI GACnn'nnGTC
Cuts at: 224 224
Size: 3506
PspOMI G'GGCC_C
Cuts at: 322 322
Size: 3506
PstI C_TGCA'G
Cuts at: 350 362 553 1238 350
Size: 12 191 685 2618
Fragments arranged by size:
2618 685 191 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3506
PvuII CAG'CTG
Cuts at: 931 1291 931
Size: 360 3146
RsrII CG'GwC_CG
Cuts at: 1701 1701
Size: 3506
SacI G_AGCT'C
Cuts at: 520 520
Size: 3506
SacII CC_GC'GG
Cuts at: 383 383
Size: 3506
SalI G'TCGA_C
Cuts at: 543 543
Size: 3506
SapI GCTCTTCn'nnn_
Cuts at: 1529 1739 1529
Size: 210 3296
SbfI CC_TGCA'GG
Cuts at: 362 553 362
Size: 191 3315
ScaI AGT'ACT
Cuts at: 3023 3023
Size: 3506
SfcI C'TryA_G
Cuts at: 33 346 358 549 1234 2227 2418 33
Size: 313 12 191 685 993 191 1121
Fragments arranged by size:
1121 993 685 313 191 191 12
SfoI GGC'GCC
Cuts at: 445 938 1186 445
Size: 493 248 2765
Fragments arranged by size:
2765 493 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3506
SmaI CCC'GGG
Cuts at: 329 528 329
Size: 199 3307
SmlI C'TyrA_G
Cuts at: 661 1576 2068 2330 661
Size: 915 492 262 1837
Fragments arranged by size:
1837 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3506
SphI G_CATG'C
Cuts at: 559 1059 559
Size: 500 3006
StyI C'CwwG_G
Cuts at: 395 640 994 395
Size: 245 354 2907
Fragments arranged by size:
2907 354 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1089 1222 1681 1890 1089
Size: 133 459 209 2705
Fragments arranged by size:
2705 459 209 133
TatI w'GTAC_w
Cuts at: 3021 3021
Size: 3506
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 3474
Tth111I GACn'n_nGTC
Cuts at: 1303 1303
Size: 3506
XbaI T'CTAG_A
Cuts at: 537 537
Size: 3506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1001 1001
Size: 3506
XmaI C'CCGG_G
Cuts at: 327 526 327
Size: 199 3307
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI