Restriction map for
pF1KB7183
(Circular) (Six-base) MAPSORT of: pF1KB7183.seq Check: 1316 from: 1 to: 4622
pF1KB7183 4622 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 305 305
Size: 4622
AatII G_ACGT'C
Cuts at: 671 671
Size: 4622
AccI GT'mk_AC
Cuts at: 1559 1660 1559
Size: 101 4521
Acc65I G'GTAC_C
Cuts at: 1638 1638
Size: 4622
AclI AA'CG_TT
Cuts at: 1616 4462 1616
Size: 2846 1776
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 1267 1531 2447 2879 3626 3894 643
Size: 624 264 916 432 747 268 1371
Fragments arranged by size:
1371 916 747 624 432 268 264
AfeI AGC'GCT
Cuts at: 1387 1387
Size: 4622
AflIII A'CryG_T
Cuts at: 1974 3078 4055 1974
Size: 1104 977 2541
Fragments arranged by size:
2541 1104 977
AgeI A'CCGG_T
Cuts at: 3010 3010
Size: 4622
AleI CACnn'nnGTG
Cuts at: 485 485
Size: 4622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 150 150
Size: 4622
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 781 813 781
Size: 32 4590
AlwNI CAG_nnn'CTG
Cuts at: 330 621 939 1125 1927 3494 330
Size: 291 318 186 802 1567 1458
Fragments arranged by size:
1567 1458 802 318 291 186
ApaI G_GGCC'C
Cuts at: 201 338 201
Size: 137 4485
ApaLI G'TGCA_C
Cuts at: 3392 3392
Size: 4622
ApoI r'AATT_y
Cuts at: 1626 3975 4231 1626
Size: 2349 256 2017
Fragments arranged by size:
2349 2017 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4622
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
AvaI C'yCGr_G
Cuts at: 194 647 1359 1459 1642 194
Size: 453 712 100 183 3174
Fragments arranged by size:
3174 712 453 183 100
AvrII C'CTAG_G
Cuts at: 202 202
Size: 4622
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 900 933 900
Size: 33 4589
BamHI G'GATC_C
Cuts at: 1647 1647
Size: 4622
BanI G'GyrC_C
Cuts at: 1466 1638 2052 2300 2335 1466
Size: 172 414 248 35 3753
Fragments arranged by size:
3753 414 248 172 35
BanII G_rGCy'C
Cuts at: 201 338 452 986 1270 1408 1636 2666 201
Size: 137 114 534 284 138 228 1030 2157
Fragments arranged by size:
2157 1030 534 284 228 138 137 114
BbeI G_GCGC'C
Cuts at: 1470 2056 2304 1470
Size: 586 248 3788
Fragments arranged by size:
3788 586 248
BbvCI CC'TCA_GC
Cuts at: 256 256
Size: 4622
BciVI GTATCCnnnnn_n'
Cuts at: 928 2520 3281 4622 928
Size: 1592 761 1341 928
Fragments arranged by size:
1592 1341 928 761
BclI T'GATC_A
Cuts at: 2142 2142
Size: 4622
BglI GCCn_nnn'nGGC
Cuts at: 196 516 743 1021 196
Size: 320 227 278 3797
Fragments arranged by size:
3797 320 278 227
BglII A'GATC_T
Cuts at: 269 2137 4174 269
Size: 1868 2037 717
Fragments arranged by size:
2037 1868 717
BlpI GC'TnA_GC
Cuts at: 1734 1734
Size: 4622
Bme1580I G_kGCm'C
Cuts at: 171 177 184 201 338 482 1433 2247 2340
Size: 6 7 17 137 144 951 814 93
Cuts at: 2340 3396 171
Size: 1056 1397
Fragments arranged by size:
1397 1056 951 814 144 137 93 17
7 6
BmgBI CAC'GTC
Cuts at: 1113 1586 1113
Size: 473 4149
BmrI ACTGGGnnnn_n'
Cuts at: 187 749 1834 1915 2250 4394 187
Size: 562 1085 81 335 2144 415
Fragments arranged by size:
2144 1085 562 415 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 857 2989 857
Size: 2132 2490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 325 466 808 294
Size: 31 141 342 4108
Fragments arranged by size:
4108 342 141 31
Bpu10I CC'TnA_GC
Cuts at: 256 1367 256
Size: 1111 3511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1198 1523 1798 2677 3169 3467 1198
Size: 325 275 879 492 298 2353
Fragments arranged by size:
2353 879 492 325 298 275
BsaI GGTCTCn'nnnn_
Cuts at: 1213 4326 1213
Size: 3113 1509
BsaAI yAC'GTr
Cuts at: 1428 1506 1975 1428
Size: 78 469 4075
Fragments arranged by size:
4075 469 78
BsaBI GATnn'nnATC
Cuts at: 6 2136 4173 6
Size: 2130 2037 455
Fragments arranged by size:
2130 2037 455
BsaHI Gr'CG_yC
Cuts at: 668 1467 2053 2301 3003 4499 668
Size: 799 586 248 702 1496 791
Fragments arranged by size:
1496 799 791 702 586 248
BsaWI w'CCGG_w
Cuts at: 1811 2332 3010 3284 3431 3842 1811
Size: 521 678 274 147 411 2591
Fragments arranged by size:
2591 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 781 811 973 1003 781
Size: 30 162 30 4400
Fragments arranged by size:
4400 162 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1585 1585
Size: 4622
BseYI C'CCAG_C
Cuts at: 233 330 676 732 1299 1303 1335 1382 2047
Size: 97 346 56 567 4 32 47 665
Cuts at: 2047 2805 3382 3878 233
Size: 758 577 496 977
Fragments arranged by size:
977 758 665 577 567 496 346 97
56 47 32 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 589 1135 1483 283
Size: 306 546 348 3422
Fragments arranged by size:
3422 546 348 306
BsiEI CG_ry'CG
Cuts at: 104 2164 2210 3418 104
Size: 2060 46 1208 1308
Fragments arranged by size:
2060 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 296 875 986 1270 1636 2414 2604 3396 296
Size: 579 111 284 366 778 190 792 1522
Fragments arranged by size:
1522 792 778 579 366 284 190 111
Bsp1286I G_dGCh'C
Cuts at: 171 177 184 201 296 338 452 482 875
Size: 6 7 17 95 42 114 30 393
Cuts at: 875 986 1270 1408 1433 1636 2247 2340 2414
Size: 111 284 138 25 203 611 93 74
Cuts at: 2414 2604 2666 3396 171
Size: 190 62 730 1397
Fragments arranged by size:
1397 730 611 393 284 203 190 138
114 111 95 93 74 62 42 30
25 17 7 6
BspEI T'CCGG_A
Cuts at: 3842 3842
Size: 4622
BspHI T'CATG_A
Cuts at: 3798 3798
Size: 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 305 1672 1886 2188 2569 305
Size: 1367 214 302 381 2358
Fragments arranged by size:
2358 1367 381 302 214
BsrBI CCG'CTC
Cuts at: 50 193 729 1371 2914 2968 4452 4617 50
Size: 143 536 642 1543 54 1484 165 55
Fragments arranged by size:
1543 1484 642 536 165 143 55 54
BsrDI GCAATG_nn'
Cuts at: 1350 2534 1350
Size: 1184 3438
BsrFI r'CCGG_y
Cuts at: 2620 2801 3010 2620
Size: 181 209 4232
Fragments arranged by size:
4232 209 181
BssSI C'ACGA_G
Cuts at: 2893 3251 2893
Size: 358 4264
BstAPI GCAn_nnn'nTGC
Cuts at: 1125 1506 1999 1125
Size: 381 493 3748
Fragments arranged by size:
3748 493 381
BstBI TT'CG_AA
Cuts at: 2983 2983
Size: 4622
BstXI CCAn_nnnn'nTGG
Cuts at: 1032 1032
Size: 4622
BstYI r'GATC_y
Cuts at: 1 269 1121 1647 2114 2137 2472 2718 3730
Size: 268 852 526 467 23 335 246 1012
Cuts at: 3730 3816 3828 4174 1
Size: 86 12 346 449
Fragments arranged by size:
1012 852 526 467 449 346 335 268
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4622
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2572 2753 2916 2572
Size: 181 163 4278
Fragments arranged by size:
4278 181 163
BtsI GCAGTG_nn'
Cuts at: 602 1040 1865 602
Size: 438 825 3359
Fragments arranged by size:
3359 825 438
DraI TTT'AAA
Cuts at: 1621 1621
Size: 4622
DraIII CAC_nnn'GTG
Cuts at: 1428 1506 1428
Size: 78 4544
DrdI GACnn_nn'nnGTC
Cuts at: 2328 3186 2328
Size: 858 3764
EaeI y'GGCC_r
Cuts at: 599 744 1142 2207 2381 2772 2799 4144 599
Size: 145 398 1065 174 391 27 1345 1077
Fragments arranged by size:
1345 1077 1065 398 391 174 145 27
EagI C'GGCC_G
Cuts at: 2207 2207
Size: 4622
EarI CTCTTCn'nnn_
Cuts at: 2645 2855 2645
Size: 210 4412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 994 1012 2830 3140 3286 4432 994
Size: 18 1818 310 146 1146 1184
Fragments arranged by size:
1818 1184 1146 310 146 18
EcoICRI GAG'CTC
Cuts at: 984 1268 1634 984
Size: 284 366 3972
Fragments arranged by size:
3972 366 284
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 325 466 643 808 1267 1531 2447 2879
Size: 31 141 177 165 459 264 916 432
Cuts at: 2879 3626 3894 294
Size: 747 268 1022
Fragments arranged by size:
1022 916 747 459 432 268 264 177
165 141 31
EcoNI CCTnn'n_nnAGG
Cuts at: 991 1129 1474 991
Size: 138 345 4139
Fragments arranged by size:
4139 345 138
EcoO109I rG'GnC_Cy
Cuts at: 198 334 618 1761 198
Size: 136 284 1143 3059
Fragments arranged by size:
3059 1143 284 136
EcoRI G'AATT_C
Cuts at: 1626 1626
Size: 4622
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 891 1239 2641 891
Size: 348 1402 2872
Fragments arranged by size:
2872 1402 348
FspI TGC'GCA
Cuts at: 2403 2403
Size: 4622
HaeII r_GCGC'y
Cuts at: 148 522 1389 1470 2056 2304 3326 4305 148
Size: 374 867 81 586 248 1022 979 465
Fragments arranged by size:
1022 979 867 586 465 374 248 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 538 570 1521 1553 2464 2494 2496 2526 538
Size: 32 951 32 911 30 2 30 2634
Fragments arranged by size:
2634 951 911 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1661 1661
Size: 4622
KasI G'GCGC_C
Cuts at: 1466 2052 2300 1466
Size: 586 248 3788
Fragments arranged by size:
3788 586 248
KpnI G_GTAC'C
Cuts at: 1642 1642
Size: 4622
MluI A'CGCG_T
Cuts at: 4055 4055
Size: 4622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3293 3477 3293
Size: 184 4438
MscI TGG'CCA
Cuts at: 601 746 2383 601
Size: 145 1637 2840
Fragments arranged by size:
2840 1637 145
MslI CAynn'nnrTG
Cuts at: 485 823 1552 2611 2738 485
Size: 338 729 1059 127 2369
Fragments arranged by size:
2369 1059 729 338 127
MspA1I CmG'CkG
Cuts at: 1303 1733 2047 2407 3420 3665 1303
Size: 430 314 360 1013 245 2260
Fragments arranged by size:
2260 1013 430 360 314 245
NaeI GCC'GGC
Cuts at: 2803 2803
Size: 4622
NarI GG'CG_CC
Cuts at: 1467 2053 2301 1467
Size: 586 248 3788
Fragments arranged by size:
3788 586 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4622
NgoMIV G'CCGG_C
Cuts at: 2801 2801
Size: 4622
NspI r_CATG'y
Cuts at: 1449 1675 2175 3082 1449
Size: 226 500 907 2989
Fragments arranged by size:
2989 907 500 226
PasI CC'CwG_GG
Cuts at: 452 452
Size: 4622
PciI A'CATG_T
Cuts at: 3078 3078
Size: 4622
PflMI CCAn_nnn'nTGG
Cuts at: 330 786 863 939 330
Size: 456 77 76 4013
Fragments arranged by size:
4013 456 77 76
PfoI T'CCnGG_A
Cuts at: 323 443 323
Size: 120 4502
PmeI GTTT'AAAC
Cuts at: 1621 1621
Size: 4622
PmlI CAC'GTG
Cuts at: 1428 1506 1428
Size: 78 4544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 781 813 3761 3793 781
Size: 32 2948 32 1610
Fragments arranged by size:
2948 1610 32 32
PspOMI G'GGCC_C
Cuts at: 197 334 197
Size: 137 4485
PstI C_TGCA'G
Cuts at: 269 436 641 1047 1669 2354 269
Size: 167 205 406 622 685 2537
Fragments arranged by size:
2537 685 622 406 205 167
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4622
PvuII CAG'CTG
Cuts at: 1303 2047 2407 1303
Size: 744 360 3518
Fragments arranged by size:
3518 744 360
RsrII CG'GwC_CG
Cuts at: 2817 2817
Size: 4622
SacI G_AGCT'C
Cuts at: 986 1270 1636 986
Size: 284 366 3972
Fragments arranged by size:
3972 366 284
SalI G'TCGA_C
Cuts at: 1659 1659
Size: 4622
SapI GCTCTTCn'nnn_
Cuts at: 2645 2855 2645
Size: 210 4412
SbfI CC_TGCA'GG
Cuts at: 436 1669 436
Size: 1233 3389
ScaI AGT'ACT
Cuts at: 4139 4139
Size: 4622
SfcI C'TryA_G
Cuts at: 33 265 432 462 637 1043 1560 1665 2350
Size: 232 167 30 175 406 517 105 685
Cuts at: 2350 3343 3534 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 517 406 232 191 175
167 105 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 743 743
Size: 4622
SfoI GGC'GCC
Cuts at: 1468 2054 2302 1468
Size: 586 248 3788
Fragments arranged by size:
3788 586 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
SmaI CCC'GGG
Cuts at: 649 1644 649
Size: 995 3627
SmlI C'TyrA_G
Cuts at: 1213 1538 1777 2692 3184 3446 1213
Size: 325 239 915 492 262 2389
Fragments arranged by size:
2389 915 492 325 262 239
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4622
SphI G_CATG'C
Cuts at: 1449 1675 2175 1449
Size: 226 500 3896
Fragments arranged by size:
3896 500 226
StuI AGG'CCT
Cuts at: 883 1365 1578 883
Size: 482 213 3927
Fragments arranged by size:
3927 482 213
StyI C'CwwG_G
Cuts at: 107 202 560 857 1756 2110 107
Size: 95 358 297 899 354 2619
Fragments arranged by size:
2619 899 358 354 297 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 444 2205 2338 2797 3006 444
Size: 1761 133 459 209 2060
Fragments arranged by size:
2060 1761 459 209 133
TatI w'GTAC_w
Cuts at: 4137 4137
Size: 4622
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4139 4171 4139
Size: 32 4590
Tth111I GACn'n_nGTC
Cuts at: 2419 2419
Size: 4622
XbaI T'CTAG_A
Cuts at: 1653 1653
Size: 4622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 248 1300 1432 2117 248
Size: 1052 132 685 2753
Fragments arranged by size:
2753 1052 685 132
XmaI C'CCGG_G
Cuts at: 647 1642 647
Size: 995 3627
XmnI GAAnn'nnTTC
Cuts at: 1516 1516
Size: 4622
ZraI GAC'GTC
Cuts at: 669 669
Size: 4622
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AhdI AscI BbsI BcgI BfrBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI SwaI XhoI