Restriction map for
pF1KB7180
(Circular) (Six-base) MAPSORT of: pF1KB7180.seq Check: 6255 from: 1 to: 4373
pF1KB7180 4373 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1333 1333
Size: 4373
AccI GT'mk_AC
Cuts at: 1411 1411
Size: 4373
Acc65I G'GTAC_C
Cuts at: 1389 1389
Size: 4373
AclI AA'CG_TT
Cuts at: 4213 4213
Size: 4373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 767 2198 2630 3377 3645 767
Size: 1431 432 747 268 1495
Fragments arranged by size:
1495 1431 747 432 268
AfeI AGC'GCT
Cuts at: 904 904
Size: 4373
AflIII A'CryG_T
Cuts at: 367 1725 2829 3806 367
Size: 1358 1104 977 934
Fragments arranged by size:
1358 1104 977 934
AgeI A'CCGG_T
Cuts at: 2761 2761
Size: 4373
AlwNI CAG_nnn'CTG
Cuts at: 302 428 1678 3245 302
Size: 126 1250 1567 1430
Fragments arranged by size:
1567 1430 1250 126
ApaI G_GGCC'C
Cuts at: 334 612 1146 334
Size: 278 534 3561
Fragments arranged by size:
3561 534 278
ApaLI G'TGCA_C
Cuts at: 3143 3143
Size: 4373
ApoI r'AATT_y
Cuts at: 1377 3726 3982 1377
Size: 2349 256 1768
Fragments arranged by size:
2349 1768 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4373
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
AvaI C'yCGr_G
Cuts at: 322 333 1393 322
Size: 11 1060 3302
Fragments arranged by size:
3302 1060 11
BamHI G'GATC_C
Cuts at: 1398 1398
Size: 4373
BanI G'GyrC_C
Cuts at: 475 1152 1281 1389 1803 2051 2086 475
Size: 677 129 108 414 248 35 2762
Fragments arranged by size:
2762 677 414 248 129 108 35
BanII G_rGCy'C
Cuts at: 334 388 612 1146 1187 1387 2417 334
Size: 54 224 534 41 200 1030 2290
Fragments arranged by size:
2290 1030 534 224 200 54 41
BbeI G_GCGC'C
Cuts at: 1285 1807 2055 1285
Size: 522 248 3603
Fragments arranged by size:
3603 522 248
BbsI GAAGACnn'nnnn_
Cuts at: 691 691
Size: 4373
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1318 1352 1318
Size: 34 4339
BciVI GTATCCnnnnn_n'
Cuts at: 2271 3032 4373 2271
Size: 761 1341 2271
Fragments arranged by size:
2271 1341 761
BclI T'GATC_A
Cuts at: 1893 1893
Size: 4373
BglI GCCn_nnn'nGGC
Cuts at: 1111 1130 1150 1279 1111
Size: 19 20 129 4205
Fragments arranged by size:
4205 129 20 19
BglII A'GATC_T
Cuts at: 1888 3925 1888
Size: 2037 2336
BlpI GC'TnA_GC
Cuts at: 599 1485 599
Size: 886 3487
Bme1580I G_kGCm'C
Cuts at: 334 612 1096 1146 1998 2091 3147 334
Size: 278 484 50 852 93 1056 1560
Fragments arranged by size:
1560 1056 852 484 278 93 50
BmgBI CAC'GTC
Cuts at: 368 368
Size: 4373
BmrI ACTGGGnnnn_n'
Cuts at: 1227 1585 1666 2001 4145 1227
Size: 358 81 335 2144 1455
Fragments arranged by size:
2144 1455 358 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 4373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 373
Size: 4373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1549 2428 2920 3218 1549
Size: 879 492 298 2704
Fragments arranged by size:
2704 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4077 4077
Size: 4373
BsaAI yAC'GTr
Cuts at: 1726 1726
Size: 4373
BsaBI GATnn'nnATC
Cuts at: 6 1887 3924 6
Size: 1881 2037 455
Fragments arranged by size:
2037 1881 455
BsaHI Gr'CG_yC
Cuts at: 292 624 877 983 1282 1804 2052 2754 4250
Size: 332 253 106 299 522 248 702 1496
Cuts at: 4250 292
Size: 415
Fragments arranged by size:
1496 702 522 415 332 299 253 248
106
BsaWI w'CCGG_w
Cuts at: 1562 2083 2761 3035 3182 3593 1562
Size: 521 678 274 147 411 2342
Fragments arranged by size:
2342 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 973 1003 973
Size: 30 4343
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1042 1042
Size: 4373
BseYI C'CCAG_C
Cuts at: 327 452 918 927 962 1170 1798 2556 3133
Size: 125 466 9 35 208 628 758 577
Cuts at: 3133 3629 327
Size: 496 1071
Fragments arranged by size:
1071 758 628 577 496 466 208 125
35 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1305
Size: 4373
BsiEI CG_ry'CG
Cuts at: 104 473 846 954 1055 1134 1251 1915 1961
Size: 369 373 108 101 79 117 664 46
Cuts at: 1961 3169 104
Size: 1208 1308
Fragments arranged by size:
1308 1208 664 373 369 117 108 101
79 46
BsiHKAI G_wGCw'C
Cuts at: 422 1341 1387 2165 2355 3147 422
Size: 919 46 778 190 792 1648
Fragments arranged by size:
1648 919 792 778 190 46
BsmI GAATG_Cn'
Cuts at: 1199 1199
Size: 4373
BsmBI CGTCTCn'nnnn_
Cuts at: 615 687 615
Size: 72 4301
Bsp1286I G_dGCh'C
Cuts at: 334 388 422 612 1096 1146 1187 1341 1387
Size: 54 34 190 484 50 41 154 46
Cuts at: 1387 1998 2091 2165 2355 2417 3147 334
Size: 611 93 74 190 62 730 1560
Fragments arranged by size:
1560 730 611 484 190 190 154 93
74 62 54 50 46 41 34
BspEI T'CCGG_A
Cuts at: 3593 3593
Size: 4373
BspHI T'CATG_A
Cuts at: 3549 3549
Size: 4373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 671 1271 1333 1423 1637 1939 2320 671
Size: 600 62 90 214 302 381 2724
Fragments arranged by size:
2724 600 381 302 214 90 62
BsrBI CCG'CTC
Cuts at: 50 1224 1253 1344 2665 2719 4203 4368 50
Size: 1174 29 91 1321 54 1484 165 55
Fragments arranged by size:
1484 1321 1174 165 91 55 54 29
BsrDI GCAATG_nn'
Cuts at: 2285 2285
Size: 4373
BsrFI r'CCGG_y
Cuts at: 465 472 949 2371 2552 2761 465
Size: 7 477 1422 181 209 2077
Fragments arranged by size:
2077 1422 477 209 181 7
BssHII G'CGCG_C
Cuts at: 253 253
Size: 4373
BssSI C'ACGA_G
Cuts at: 1334 2644 3002 1334
Size: 1310 358 2705
Fragments arranged by size:
2705 1310 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1079 1750 1079
Size: 671 3702
BstBI TT'CG_AA
Cuts at: 2734 2734
Size: 4373
BstXI CCAn_nnnn'nTGG
Cuts at: 1170 1285 1170
Size: 115 4258
BstYI r'GATC_y
Cuts at: 1 298 1398 1865 1888 2223 2469 3481 3567
Size: 297 1100 467 23 335 246 1012 86
Cuts at: 3567 3579 3925 1
Size: 12 346 449
Fragments arranged by size:
1100 1012 467 449 346 335 297 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 535 535
Size: 4373
BtgI C'CryG_G
Cuts at: 311 647 1115 1125 1192 311
Size: 336 468 10 67 3492
Fragments arranged by size:
3492 468 336 67 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2323 2504 2667 2323
Size: 181 163 4029
Fragments arranged by size:
4029 181 163
BtsI GCAGTG_nn'
Cuts at: 957 1049 1616 957
Size: 92 567 3714
Fragments arranged by size:
3714 567 92
DraI TTT'AAA
Cuts at: 1372 1372
Size: 4373
DrdI GACnn_nn'nnGTC
Cuts at: 2079 2937 2079
Size: 858 3515
EaeI y'GGCC_r
Cuts at: 951 1052 1131 1248 1958 2132 2523 2550 3895
Size: 101 79 117 710 174 391 27 1345
Cuts at: 3895 951
Size: 1429
Fragments arranged by size:
1429 1345 710 391 174 117 101 79
27
EagI C'GGCC_G
Cuts at: 951 1052 1131 1248 1958 951
Size: 101 79 117 710 3366
Fragments arranged by size:
3366 710 117 101 79
EarI CTCTTCn'nnn_
Cuts at: 142 1008 2396 2606 142
Size: 866 1388 210 1909
Fragments arranged by size:
1909 1388 866 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 189 452 828 2581 2891 3037 4183 189
Size: 263 376 1753 310 146 1146 379
Fragments arranged by size:
1753 1146 379 376 310 263 146
EcoICRI GAG'CTC
Cuts at: 1385 1385
Size: 4373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 767 2198 2630 3377 3645 373
Size: 394 1431 432 747 268 1101
Fragments arranged by size:
1431 1101 747 432 394 268
EcoO109I rG'GnC_Cy
Cuts at: 396 608 1143 1512 396
Size: 212 535 369 3257
Fragments arranged by size:
3257 535 369 212
EcoRI G'AATT_C
Cuts at: 1377 1377
Size: 4373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2392 2392
Size: 4373
FseI GG_CCGG'CC
Cuts at: 953 953
Size: 4373
FspI TGC'GCA
Cuts at: 935 2154 935
Size: 1219 3154
HaeII r_GCGC'y
Cuts at: 906 1285 1807 2055 3077 4056 906
Size: 379 522 248 1022 979 1223
Fragments arranged by size:
1223 1022 979 522 379 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 122 143 154 175 357 389 689 721 973
Size: 21 11 21 182 32 300 32 252
Cuts at: 973 1005 1237 1269 2215 2245 2247 2277 122
Size: 32 232 32 946 30 2 30 2218
Fragments arranged by size:
2218 946 300 252 232 182 32 32
32 32 30 30 21 21 11 2
HincII GTy'rAC
Cuts at: 1412 1412
Size: 4373
KasI G'GCGC_C
Cuts at: 1281 1803 2051 1281
Size: 522 248 3603
Fragments arranged by size:
3603 522 248
KpnI G_GTAC'C
Cuts at: 1393 1393
Size: 4373
MluI A'CGCG_T
Cuts at: 3806 3806
Size: 4373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 183 3044 3228 183
Size: 2861 184 1328
Fragments arranged by size:
2861 1328 184
MscI TGG'CCA
Cuts at: 2134 2134
Size: 4373
MslI CAynn'nnrTG
Cuts at: 1204 2362 2489 1204
Size: 1158 127 3088
Fragments arranged by size:
3088 1158 127
MspA1I CmG'CkG
Cuts at: 360 452 922 1127 1194 1314 1360 1484 1798
Size: 92 470 205 67 120 46 124 314
Cuts at: 1798 2158 3171 3416 360
Size: 360 1013 245 1317
Fragments arranged by size:
1317 1013 470 360 314 245 205 124
120 92 67 46
NaeI GCC'GGC
Cuts at: 467 951 2554 467
Size: 484 1603 2286
Fragments arranged by size:
2286 1603 484
NarI GG'CG_CC
Cuts at: 1282 1804 2052 1282
Size: 522 248 3603
Fragments arranged by size:
3603 522 248
NcoI C'CATG_G
Cuts at: 311 647 311
Size: 336 4037
NgoMIV G'CCGG_C
Cuts at: 465 949 2552 465
Size: 484 1603 2286
Fragments arranged by size:
2286 1603 484
NotI GC'GGCC_GC
Cuts at: 1131 1131
Size: 4373
NspI r_CATG'y
Cuts at: 1426 1926 2833 1426
Size: 500 907 2966
Fragments arranged by size:
2966 907 500
PasI CC'CwG_GG
Cuts at: 808 1293 808
Size: 485 3888
PciI A'CATG_T
Cuts at: 2829 2829
Size: 4373
PflMI CCAn_nnn'nTGG
Cuts at: 113 302 113
Size: 189 4184
PmeI GTTT'AAAC
Cuts at: 1372 1372
Size: 4373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 3512
Size: 32 4341
PpuMI rG'GwC_Cy
Cuts at: 396 396
Size: 4373
PspOMI G'GGCC_C
Cuts at: 330 608 1142 330
Size: 278 534 3561
Fragments arranged by size:
3561 534 278
PstI C_TGCA'G
Cuts at: 997 1420 2105 997
Size: 423 685 3265
Fragments arranged by size:
3265 685 423
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4373
PvuII CAG'CTG
Cuts at: 360 922 1798 2158 360
Size: 562 876 360 2575
Fragments arranged by size:
2575 876 562 360
RsrII CG'GwC_CG
Cuts at: 2568 2568
Size: 4373
SacI G_AGCT'C
Cuts at: 1387 1387
Size: 4373
SacII CC_GC'GG
Cuts at: 1128 1195 1128
Size: 67 4306
SalI G'TCGA_C
Cuts at: 1410 1410
Size: 4373
SapI GCTCTTCn'nnn_
Cuts at: 2396 2606 2396
Size: 210 4163
SbfI CC_TGCA'GG
Cuts at: 1420 1420
Size: 4373
ScaI AGT'ACT
Cuts at: 3890 3890
Size: 4373
SfcI C'TryA_G
Cuts at: 33 993 1416 2101 3094 3285 33
Size: 960 423 685 993 191 1121
Fragments arranged by size:
1121 993 960 685 423 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1111 1130 1111
Size: 19 4354
SfoI GGC'GCC
Cuts at: 1283 1805 2053 1283
Size: 522 248 3603
Fragments arranged by size:
3603 522 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
SgrAI Cr'CCGG_yG
Cuts at: 465 465
Size: 4373
SmaI CCC'GGG
Cuts at: 335 1395 335
Size: 1060 3313
SmlI C'TyrA_G
Cuts at: 1528 2443 2935 3197 1528
Size: 915 492 262 2704
Fragments arranged by size:
2704 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4373
SphI G_CATG'C
Cuts at: 1426 1926 1426
Size: 500 3873
StyI C'CwwG_G
Cuts at: 311 647 1507 1861 311
Size: 336 860 354 2823
Fragments arranged by size:
2823 860 354 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 919 1956 2089 2548 2757 919
Size: 1037 133 459 209 2535
Fragments arranged by size:
2535 1037 459 209 133
TatI w'GTAC_w
Cuts at: 3888 3888
Size: 4373
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 483 515 3890 3922 483
Size: 32 3375 32 934
Fragments arranged by size:
3375 934 32 32
Tth111I GACn'n_nGTC
Cuts at: 2170 2170
Size: 4373
XbaI T'CTAG_A
Cuts at: 1404 1404
Size: 4373
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 654 1868 654
Size: 1214 3159
XmaI C'CCGG_G
Cuts at: 333 1393 333
Size: 1060 3313
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmtI Bpu10I BsiWI BsrGI BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspXI PsrI SanDI SexAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI