Restriction map for
pF1KB7178
(Circular) (Six-base) MAPSORT of: pF1KB7178.seq Check: 8156 from: 1 to: 4391
pF1KB7178 4391 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 417 1429 417
Size: 1012 3379
Acc65I G'GTAC_C
Cuts at: 1407 1407
Size: 4391
AclI AA'CG_TT
Cuts at: 4231 4231
Size: 4391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 2216 2648 3395 3663 1006
Size: 1210 432 747 268 1734
Fragments arranged by size:
1734 1210 747 432 268
AflII C'TTAA_G
Cuts at: 519 519
Size: 4391
AflIII A'CryG_T
Cuts at: 1743 2847 3824 1743
Size: 1104 977 2310
Fragments arranged by size:
2310 1104 977
AgeI A'CCGG_T
Cuts at: 2779 2779
Size: 4391
AleI CACnn'nnGTG
Cuts at: 997 997
Size: 4391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 668 668
Size: 4391
AlwNI CAG_nnn'CTG
Cuts at: 1038 1696 3263 1038
Size: 658 1567 2166
Fragments arranged by size:
2166 1567 658
ApaI G_GGCC'C
Cuts at: 765 765
Size: 4391
ApaLI G'TGCA_C
Cuts at: 3161 3161
Size: 4391
ApoI r'AATT_y
Cuts at: 165 1395 3744 4000 165
Size: 1230 2349 256 556
Fragments arranged by size:
2349 1230 556 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4391
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
AvaI C'yCGr_G
Cuts at: 848 1411 848
Size: 563 3828
BamHI G'GATC_C
Cuts at: 1416 1416
Size: 4391
BanI G'GyrC_C
Cuts at: 115 931 1407 1821 2069 2104 115
Size: 816 476 414 248 35 2402
Fragments arranged by size:
2402 816 476 414 248 35
BanII G_rGCy'C
Cuts at: 765 1405 2435 765
Size: 640 1030 2721
Fragments arranged by size:
2721 1030 640
BbeI G_GCGC'C
Cuts at: 935 1825 2073 935
Size: 890 248 3253
Fragments arranged by size:
3253 890 248
BbsI GAAGACnn'nnnn_
Cuts at: 317 370 619 317
Size: 53 249 4089
Fragments arranged by size:
4089 249 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 826 860 826
Size: 34 4357
BciVI GTATCCnnnnn_n'
Cuts at: 2289 3050 4391 2289
Size: 761 1341 2289
Fragments arranged by size:
2289 1341 761
BclI T'GATC_A
Cuts at: 1911 1911
Size: 4391
BglI GCCn_nnn'nGGC
Cuts at: 760 760
Size: 4391
BglII A'GATC_T
Cuts at: 1022 1906 3943 1022
Size: 884 2037 1470
Fragments arranged by size:
2037 1470 884
BlpI GC'TnA_GC
Cuts at: 1503 1503
Size: 4391
Bme1580I G_kGCm'C
Cuts at: 765 2016 2109 3165 765
Size: 1251 93 1056 1991
Fragments arranged by size:
1991 1251 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 768 1215 1603 1684 2019 4163 768
Size: 447 388 81 335 2144 996
Fragments arranged by size:
2144 996 447 388 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 4391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 161
Size: 4391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1567 2446 2938 3236 193
Size: 1374 879 492 298 1348
Fragments arranged by size:
1374 1348 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 727 4095 727
Size: 3368 1023
BsaAI yAC'GTr
Cuts at: 444 690 903 981 1744 444
Size: 246 213 78 763 3091
Fragments arranged by size:
3091 763 246 213 78
BsaBI GATnn'nnATC
Cuts at: 6 1905 3942 6
Size: 1899 2037 455
Fragments arranged by size:
2037 1899 455
BsaHI Gr'CG_yC
Cuts at: 637 932 1822 2070 2772 4268 637
Size: 295 890 248 702 1496 760
Fragments arranged by size:
1496 890 760 702 295 248
BsaWI w'CCGG_w
Cuts at: 1580 2101 2779 3053 3200 3611 1580
Size: 521 678 274 147 411 2360
Fragments arranged by size:
2360 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 769 799 769
Size: 30 4361
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 646 646
Size: 4391
BseYI C'CCAG_C
Cuts at: 1013 1816 2574 3151 3647 1013
Size: 803 758 577 496 1757
Fragments arranged by size:
1757 803 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1933 1979 3187 104
Size: 1829 46 1208 1308
Fragments arranged by size:
1829 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 800 908 1405 2183 2373 3165 800
Size: 108 497 778 190 792 2026
Fragments arranged by size:
2026 792 778 497 190 108
BsmI GAATG_Cn'
Cuts at: 726 726
Size: 4391
BsmBI CGTCTCn'nnnn_
Cuts at: 628 628
Size: 4391
Bsp1286I G_dGCh'C
Cuts at: 765 800 908 1405 2016 2109 2183 2373 2435
Size: 35 108 497 611 93 74 190 62
Cuts at: 2435 3165 765
Size: 730 1991
Fragments arranged by size:
1991 730 611 497 190 108 93 74
62 35
BspEI T'CCGG_A
Cuts at: 3611 3611
Size: 4391
BspHI T'CATG_A
Cuts at: 3567 3567
Size: 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1441 1655 1957 2338 1441
Size: 214 302 381 3494
Fragments arranged by size:
3494 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2683 2737 4221 4386 50
Size: 2633 54 1484 165 55
Fragments arranged by size:
2633 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2303 2303
Size: 4391
BsrFI r'CCGG_y
Cuts at: 2389 2570 2779 2389
Size: 181 209 4001
Fragments arranged by size:
4001 209 181
BsrGI T'GTAC_A
Cuts at: 547 547
Size: 4391
BssSI C'ACGA_G
Cuts at: 2662 3020 2662
Size: 358 4033
BstAPI GCAn_nnn'nTGC
Cuts at: 918 1768 918
Size: 850 3541
BstBI TT'CG_AA
Cuts at: 2752 2752
Size: 4391
BstEII G'GTnAC_C
Cuts at: 990 990
Size: 4391
BstXI CCAn_nnnn'nTGG
Cuts at: 582 948 582
Size: 366 4025
BstYI r'GATC_y
Cuts at: 1 479 1022 1416 1883 1906 2241 2487 3499
Size: 478 543 394 467 23 335 246 1012
Cuts at: 3499 3585 3597 3943 1
Size: 86 12 346 449
Fragments arranged by size:
1012 543 478 467 449 394 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1047 107
Size: 940 3451
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 732 2341 2522 2685 732
Size: 1609 181 163 2438
Fragments arranged by size:
2438 1609 181 163
BtsI GCAGTG_nn'
Cuts at: 119 1634 119
Size: 1515 2876
DraI TTT'AAA
Cuts at: 864 1390 864
Size: 526 3865
DraIII CAC_nnn'GTG
Cuts at: 1166 1166
Size: 4391
DrdI GACnn_nn'nnGTC
Cuts at: 2097 2955 2097
Size: 858 3533
EaeI y'GGCC_r
Cuts at: 363 458 743 1976 2150 2541 2568 3913 363
Size: 95 285 1233 174 391 27 1345 841
Fragments arranged by size:
1345 1233 841 391 285 174 95 27
EagI C'GGCC_G
Cuts at: 1976 1976
Size: 4391
EarI CTCTTCn'nnn_
Cuts at: 443 1167 2414 2624 443
Size: 724 1247 210 2210
Fragments arranged by size:
2210 1247 724 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2599 2909 3055 4201 2599
Size: 310 146 1146 2789
Fragments arranged by size:
2789 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1403 1403
Size: 4391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 1006 2216 2648 3395 3663 161
Size: 845 1210 432 747 268 889
Fragments arranged by size:
1210 889 845 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 877 877
Size: 4391
EcoO109I rG'GnC_Cy
Cuts at: 762 1530 762
Size: 768 3623
EcoRI G'AATT_C
Cuts at: 1395 1395
Size: 4391
EcoRV GAT'ATC
Cuts at: 954 954
Size: 4391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4391
FspI TGC'GCA
Cuts at: 2172 2172
Size: 4391
HaeII r_GCGC'y
Cuts at: 181 935 1825 2073 3095 4074 181
Size: 754 890 248 1022 979 498
Fragments arranged by size:
1022 979 890 754 498 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 850 882 2233 2263 2265 2295 850
Size: 32 1351 30 2 30 2946
Fragments arranged by size:
2946 1351 32 30 30 2
HincII GTy'rAC
Cuts at: 1430 1430
Size: 4391
KasI G'GCGC_C
Cuts at: 931 1821 2069 931
Size: 890 248 3253
Fragments arranged by size:
3253 890 248
KpnI G_GTAC'C
Cuts at: 1411 1411
Size: 4391
MfeI C'AATT_G
Cuts at: 507 580 1118 507
Size: 73 538 3780
Fragments arranged by size:
3780 538 73
MluI A'CGCG_T
Cuts at: 3824 3824
Size: 4391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3062 3246 3062
Size: 184 4207
MscI TGG'CCA
Cuts at: 365 460 745 2152 365
Size: 95 285 1407 2604
Fragments arranged by size:
2604 1407 285 95
MslI CAynn'nnrTG
Cuts at: 913 997 2380 2507 913
Size: 84 1383 127 2797
Fragments arranged by size:
2797 1383 127 84
MspA1I CmG'CkG
Cuts at: 1502 1816 2176 3189 3434 1502
Size: 314 360 1013 245 2459
Fragments arranged by size:
2459 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2572 2572
Size: 4391
NarI GG'CG_CC
Cuts at: 932 1822 2070 932
Size: 890 248 3253
Fragments arranged by size:
3253 890 248
NcoI C'CATG_G
Cuts at: 107 1047 107
Size: 940 3451
NgoMIV G'CCGG_C
Cuts at: 2570 2570
Size: 4391
NspI r_CATG'y
Cuts at: 819 1444 1944 2851 819
Size: 625 500 907 2359
Fragments arranged by size:
2359 907 625 500
PciI A'CATG_T
Cuts at: 2847 2847
Size: 4391
PmeI GTTT'AAAC
Cuts at: 1390 1390
Size: 4391
PmlI CAC'GTG
Cuts at: 444 903 444
Size: 459 3932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 4359
PsiI TTA'TAA
Cuts at: 287 287
Size: 4391
PspOMI G'GGCC_C
Cuts at: 761 761
Size: 4391
PstI C_TGCA'G
Cuts at: 1438 2123 1438
Size: 685 3706
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4391
PvuII CAG'CTG
Cuts at: 1816 2176 1816
Size: 360 4031
RsrII CG'GwC_CG
Cuts at: 2586 2586
Size: 4391
SacI G_AGCT'C
Cuts at: 1405 1405
Size: 4391
SalI G'TCGA_C
Cuts at: 1428 1428
Size: 4391
SapI GCTCTTCn'nnn_
Cuts at: 1167 2414 2624 1167
Size: 1247 210 2934
Fragments arranged by size:
2934 1247 210
SbfI CC_TGCA'GG
Cuts at: 1438 1438
Size: 4391
ScaI AGT'ACT
Cuts at: 1354 3908 1354
Size: 2554 1837
SexAI A'CCwGG_T
Cuts at: 803 803
Size: 4391
SfcI C'TryA_G
Cuts at: 33 414 1434 2119 3112 3303 33
Size: 381 1020 685 993 191 1121
Fragments arranged by size:
1121 1020 993 685 381 191
SfoI GGC'GCC
Cuts at: 933 1823 2071 933
Size: 890 248 3253
Fragments arranged by size:
3253 890 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
SmaI CCC'GGG
Cuts at: 1413 1413
Size: 4391
SmlI C'TyrA_G
Cuts at: 172 519 1546 2461 2953 3215 172
Size: 347 1027 915 492 262 1348
Fragments arranged by size:
1348 1027 915 492 347 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4391
SphI G_CATG'C
Cuts at: 819 1444 1944 819
Size: 625 500 3266
Fragments arranged by size:
3266 625 500
StuI AGG'CCT
Cuts at: 341 1096 341
Size: 755 3636
StyI C'CwwG_G
Cuts at: 107 1047 1249 1525 1879 107
Size: 940 202 276 354 2619
Fragments arranged by size:
2619 940 354 276 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1974 2107 2566 2775 1974
Size: 133 459 209 3590
Fragments arranged by size:
3590 459 209 133
TatI w'GTAC_w
Cuts at: 547 785 1352 3906 547
Size: 238 567 2554 1032
Fragments arranged by size:
2554 1032 567 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3908 3940 3908
Size: 32 4359
Tth111I GACn'n_nGTC
Cuts at: 2188 2188
Size: 4391
XbaI T'CTAG_A
Cuts at: 1422 1422
Size: 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1886 1886
Size: 4391
XmaI C'CCGG_G
Cuts at: 1411 1411
Size: 4391
XmnI GAAnn'nnTTC
Cuts at: 377 377
Size: 4391
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI AscI AvrII BaeI
BbvCI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI BssHII
BstZ17I Bsu36I ClaI CspCI FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PpuMI PshAI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI