Restriction map for
pF1KB7177
(Circular) (Six-base) MAPSORT of: pF1KB7177.seq Check: 3706 from: 1 to: 4559
pF1KB7177 4559 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1597 1597
Size: 4559
Acc65I G'GTAC_C
Cuts at: 1575 1575
Size: 4559
AclI AA'CG_TT
Cuts at: 4399 4399
Size: 4559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2816 3563 3831 2384
Size: 432 747 268 3112
Fragments arranged by size:
3112 747 432 268
AflIII A'CryG_T
Cuts at: 1911 3015 3992 1911
Size: 1104 977 2478
Fragments arranged by size:
2478 1104 977
AgeI A'CCGG_T
Cuts at: 2947 2947
Size: 4559
AlwNI CAG_nnn'CTG
Cuts at: 1864 3431 1864
Size: 1567 2992
ApaI G_GGCC'C
Cuts at: 124 1454 124
Size: 1330 3229
ApaLI G'TGCA_C
Cuts at: 3329 3329
Size: 4559
ApoI r'AATT_y
Cuts at: 790 1563 3912 4168 790
Size: 773 2349 256 1181
Fragments arranged by size:
2349 1181 773 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
AvaI C'yCGr_G
Cuts at: 124 237 372 1579 124
Size: 113 135 1207 3104
Fragments arranged by size:
3104 1207 135 113
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 412 445 412
Size: 33 4526
BamHI G'GATC_C
Cuts at: 1584 1584
Size: 4559
BanI G'GyrC_C
Cuts at: 241 327 441 1575 1989 2237 2272 241
Size: 86 114 1134 414 248 35 2528
Fragments arranged by size:
2528 1134 414 248 114 86 35
BanII G_rGCy'C
Cuts at: 124 379 1454 1573 2603 124
Size: 255 1075 119 1030 2080
Fragments arranged by size:
2080 1075 1030 255 119
BbeI G_GCGC'C
Cuts at: 245 331 1993 2241 245
Size: 86 1662 248 2563
Fragments arranged by size:
2563 1662 248 86
BbsI GAAGACnn'nnnn_
Cuts at: 817 1517 817
Size: 700 3859
BbvCI CC'TCA_GC
Cuts at: 255 276 255
Size: 21 4538
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 399 433 399
Size: 34 4525
BciVI GTATCCnnnnn_n'
Cuts at: 2457 3218 4559 2457
Size: 761 1341 2457
Fragments arranged by size:
2457 1341 761
BclI T'GATC_A
Cuts at: 930 2079 930
Size: 1149 3410
BfrBI ATG'CAT
Cuts at: 1543 1543
Size: 4559
BglI GCCn_nnn'nGGC
Cuts at: 202 466 202
Size: 264 4295
BglII A'GATC_T
Cuts at: 2074 4111 2074
Size: 2037 2522
BlpI GC'TnA_GC
Cuts at: 1671 1671
Size: 4559
Bme1580I G_kGCm'C
Cuts at: 124 446 1454 2184 2277 3333 124
Size: 322 1008 730 93 1056 1350
Fragments arranged by size:
1350 1056 1008 730 322 93
BmgBI CAC'GTC
Cuts at: 1329 1329
Size: 4559
BmrI ACTGGGnnnn_n'
Cuts at: 420 1448 1771 1852 2187 4331 420
Size: 1028 323 81 335 2144 648
Fragments arranged by size:
2144 1028 648 335 323 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2926 2926
Size: 4559
Bpu10I CC'TnA_GC
Cuts at: 255 276 255
Size: 21 4538
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1043 1735 2614 3106 3404 1043
Size: 692 879 492 298 2198
Fragments arranged by size:
2198 879 692 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 135 1435 4263 135
Size: 1300 2828 431
Fragments arranged by size:
2828 1300 431
BsaAI yAC'GTr
Cuts at: 1912 1912
Size: 4559
BsaBI GATnn'nnATC
Cuts at: 6 1542 2073 4110 6
Size: 1536 531 2037 455
Fragments arranged by size:
2037 1536 531 455
BsaHI Gr'CG_yC
Cuts at: 242 328 1383 1990 2238 2940 4436 242
Size: 86 1055 607 248 702 1496 365
Fragments arranged by size:
1496 1055 702 607 365 248 86
BsaWI w'CCGG_w
Cuts at: 1748 2269 2947 3221 3368 3779 1748
Size: 521 678 274 147 411 2528
Fragments arranged by size:
2528 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 243 406 886 243
Size: 163 480 3916
Fragments arranged by size:
3916 480 163
BseYI C'CCAG_C
Cuts at: 1984 2742 3319 3815 1984
Size: 758 577 496 2728
Fragments arranged by size:
2728 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 164 206 266 2101 2147 3355 104
Size: 60 42 60 1835 46 1208 1308
Fragments arranged by size:
1835 1308 1208 60 60 46 42
BsiHKAI G_wGCw'C
Cuts at: 398 1182 1573 2351 2541 3333 398
Size: 784 391 778 190 792 1624
Fragments arranged by size:
1624 792 784 778 391 190
Bsp1286I G_dGCh'C
Cuts at: 124 379 398 446 1182 1454 1573 2184 2277
Size: 255 19 48 736 272 119 611 93
Cuts at: 2277 2351 2541 2603 3333 124
Size: 74 190 62 730 1350
Fragments arranged by size:
1350 736 730 611 272 255 190 119
93 74 62 48 19
BspEI T'CCGG_A
Cuts at: 3779 3779
Size: 4559
BspHI T'CATG_A
Cuts at: 3735 3735
Size: 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1609 1823 2125 2506 1609
Size: 214 302 381 3662
Fragments arranged by size:
3662 381 302 214
BsrBI CCG'CTC
Cuts at: 50 867 2851 2905 4389 4554 50
Size: 817 1984 54 1484 165 55
Fragments arranged by size:
1984 1484 817 165 55 54
BsrDI GCAATG_nn'
Cuts at: 554 560 2471 554
Size: 6 1911 2642
Fragments arranged by size:
2642 1911 6
BsrFI r'CCGG_y
Cuts at: 2557 2738 2947 2557
Size: 181 209 4169
Fragments arranged by size:
4169 209 181
BssSI C'ACGA_G
Cuts at: 2830 3188 2830
Size: 358 4201
BstAPI GCAn_nnn'nTGC
Cuts at: 1936 1936
Size: 4559
BstBI TT'CG_AA
Cuts at: 2920 2920
Size: 4559
BstEII G'GTnAC_C
Cuts at: 512 512
Size: 4559
BstXI CCAn_nnnn'nTGG
Cuts at: 680 680
Size: 4559
BstYI r'GATC_y
Cuts at: 1 1151 1584 2051 2074 2409 2655 3667 3753
Size: 1150 433 467 23 335 246 1012 86
Cuts at: 3753 3765 4111 1
Size: 12 346 449
Fragments arranged by size:
1150 1012 467 449 433 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 980 980
Size: 4559
BtgI C'CryG_G
Cuts at: 107 1103 107
Size: 996 3563
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2509 2690 2853 2509
Size: 181 163 4215
Fragments arranged by size:
4215 181 163
BtsI GCAGTG_nn'
Cuts at: 227 1091 1802 227
Size: 864 711 2984
Fragments arranged by size:
2984 864 711
DraI TTT'AAA
Cuts at: 1558 1558
Size: 4559
DrdI GACnn_nn'nnGTC
Cuts at: 2265 3123 2265
Size: 858 3701
EaeI y'GGCC_r
Cuts at: 161 203 263 300 1302 2144 2318 2709 2736
Size: 42 60 37 1002 842 174 391 27
Cuts at: 2736 4081 161
Size: 1345 639
Fragments arranged by size:
1345 1002 842 639 391 174 60 42
37 27
EagI C'GGCC_G
Cuts at: 161 203 263 2144 161
Size: 42 60 1881 2576
Fragments arranged by size:
2576 1881 60 42
EarI CTCTTCn'nnn_
Cuts at: 616 887 1259 1433 1499 2582 2792 616
Size: 271 372 174 66 1083 210 2383
Fragments arranged by size:
2383 1083 372 271 210 174 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 159 165 180 2767 3077 3223 4369 159
Size: 6 15 2587 310 146 1146 349
Fragments arranged by size:
2587 1146 349 310 146 15 6
EcoICRI GAG'CTC
Cuts at: 1571 1571
Size: 4559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2816 3563 3831 2384
Size: 432 747 268 3112
Fragments arranged by size:
3112 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1040 1040
Size: 4559
EcoO109I rG'GnC_Cy
Cuts at: 120 121 1698 120
Size: 1 1577 2981
Fragments arranged by size:
2981 1577 1
EcoRI G'AATT_C
Cuts at: 1563 1563
Size: 4559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1210 1288 2578 1210
Size: 78 1290 3191
Fragments arranged by size:
3191 1290 78
FspI TGC'GCA
Cuts at: 2340 2340
Size: 4559
HaeII r_GCGC'y
Cuts at: 245 331 1993 2241 3263 4242 245
Size: 86 1662 248 1022 979 562
Fragments arranged by size:
1662 1022 979 562 248 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 963 995 2401 2431 2433 2463 963
Size: 32 1406 30 2 30 3059
Fragments arranged by size:
3059 1406 32 30 30 2
HincII GTy'rAC
Cuts at: 1598 1598
Size: 4559
KasI G'GCGC_C
Cuts at: 241 327 1989 2237 241
Size: 86 1662 248 2563
Fragments arranged by size:
2563 1662 248 86
KpnI G_GTAC'C
Cuts at: 1579 1579
Size: 4559
MluI A'CGCG_T
Cuts at: 3992 3992
Size: 4559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 399 524 774 1234 3230 3414 399
Size: 125 250 460 1996 184 1544
Fragments arranged by size:
1996 1544 460 250 184 125
MscI TGG'CCA
Cuts at: 302 2320 302
Size: 2018 2541
MslI CAynn'nnrTG
Cuts at: 1495 2548 2675 1495
Size: 1053 127 3379
Fragments arranged by size:
3379 1053 127
MspA1I CmG'CkG
Cuts at: 1670 1984 2344 3357 3602 1670
Size: 314 360 1013 245 2627
Fragments arranged by size:
2627 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2740 2740
Size: 4559
NarI GG'CG_CC
Cuts at: 242 328 1990 2238 242
Size: 86 1662 248 2563
Fragments arranged by size:
2563 1662 248 86
NcoI C'CATG_G
Cuts at: 107 107
Size: 4559
NgoMIV G'CCGG_C
Cuts at: 2738 2738
Size: 4559
NotI GC'GGCC_GC
Cuts at: 161 161
Size: 4559
NsiI A_TGCA'T
Cuts at: 1545 1545
Size: 4559
NspI r_CATG'y
Cuts at: 504 1612 2112 3019 504
Size: 1108 500 907 2044
Fragments arranged by size:
2044 1108 907 500
PciI A'CATG_T
Cuts at: 3015 3015
Size: 4559
PflMI CCAn_nnn'nTGG
Cuts at: 438 438
Size: 4559
PmeI GTTT'AAAC
Cuts at: 1558 1558
Size: 4559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 4527
PspOMI G'GGCC_C
Cuts at: 120 1450 120
Size: 1330 3229
PstI C_TGCA'G
Cuts at: 1606 2291 1606
Size: 685 3874
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4559
PvuII CAG'CTG
Cuts at: 1984 2344 1984
Size: 360 4199
RsrII CG'GwC_CG
Cuts at: 2754 2754
Size: 4559
SacI G_AGCT'C
Cuts at: 1573 1573
Size: 4559
SalI G'TCGA_C
Cuts at: 1596 1596
Size: 4559
SapI GCTCTTCn'nnn_
Cuts at: 616 1259 2582 2792 616
Size: 643 1323 210 2383
Fragments arranged by size:
2383 1323 643 210
SbfI CC_TGCA'GG
Cuts at: 1606 1606
Size: 4559
ScaI AGT'ACT
Cuts at: 4076 4076
Size: 4559
SfcI C'TryA_G
Cuts at: 33 773 1259 1602 2287 3280 3471 33
Size: 740 486 343 685 993 191 1121
Fragments arranged by size:
1121 993 740 685 486 343 191
SfoI GGC'GCC
Cuts at: 243 329 1991 2239 243
Size: 86 1662 248 2563
Fragments arranged by size:
2563 1662 248 86
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
SmaI CCC'GGG
Cuts at: 1581 1581
Size: 4559
SmlI C'TyrA_G
Cuts at: 1058 1714 2629 3121 3383 1058
Size: 656 915 492 262 2234
Fragments arranged by size:
2234 915 656 492 262
SpeI A'CTAG_T
Cuts at: 67 826 67
Size: 759 3800
SphI G_CATG'C
Cuts at: 1612 2112 1612
Size: 500 4059
SspI AAT'ATT
Cuts at: 1161 1161
Size: 4559
StyI C'CwwG_G
Cuts at: 107 1693 2047 107
Size: 1586 354 2619
Fragments arranged by size:
2619 1586 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 531 2142 2275 2734 2943 531
Size: 1611 133 459 209 2147
Fragments arranged by size:
2147 1611 459 209 133
TatI w'GTAC_w
Cuts at: 4074 4074
Size: 4559
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4076 4108 4076
Size: 32 4527
Tth111I GACn'n_nGTC
Cuts at: 476 2356 476
Size: 1880 2679
XbaI T'CTAG_A
Cuts at: 1590 1590
Size: 4559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2054 2054
Size: 4559
XmaI C'CCGG_G
Cuts at: 1579 1579
Size: 4559
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NsiI
NspI PciI PflMI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BmtI BpmI BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI