Restriction map for
pF1KB7173
(Circular) (Six-base) MAPSORT of: pF1KB7173.seq Check: 7859 from: 1 to: 4703
pF1KB7173 4703 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 381 381
Size: 4703
AccI GT'mk_AC
Cuts at: 1741 1741
Size: 4703
Acc65I G'GTAC_C
Cuts at: 1719 1719
Size: 4703
AclI AA'CG_TT
Cuts at: 4543 4543
Size: 4703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 991 1516 2528 2960 3707 3975 340
Size: 651 525 1012 432 747 268 1068
Fragments arranged by size:
1068 1012 747 651 525 432 268
AflIII A'CryG_T
Cuts at: 2055 3159 4136 2055
Size: 1104 977 2622
Fragments arranged by size:
2622 1104 977
AgeI A'CCGG_T
Cuts at: 3091 3091
Size: 4703
AhdI GACnn_n'nnGTC
Cuts at: 120 120
Size: 4703
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 214 246 214
Size: 32 4671
AlwNI CAG_nnn'CTG
Cuts at: 348 612 2008 3575 348
Size: 264 1396 1567 1476
Fragments arranged by size:
1567 1476 1396 264
ApaI G_GGCC'C
Cuts at: 463 707 463
Size: 244 4459
ApaLI G'TGCA_C
Cuts at: 3473 3473
Size: 4703
ApoI r'AATT_y
Cuts at: 675 1256 1707 4056 4312 675
Size: 581 451 2349 256 1066
Fragments arranged by size:
2349 1066 581 451 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4703
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
AvaI C'yCGr_G
Cuts at: 355 592 700 1723 355
Size: 237 108 1023 3335
Fragments arranged by size:
3335 1023 237 108
BamHI G'GATC_C
Cuts at: 623 1728 623
Size: 1105 3598
BanI G'GyrC_C
Cuts at: 801 861 940 1719 2133 2381 2416 801
Size: 60 79 779 414 248 35 3088
Fragments arranged by size:
3088 779 414 248 79 60 35
BanII G_rGCy'C
Cuts at: 367 419 463 647 707 1586 1717 2747 367
Size: 52 44 184 60 879 131 1030 2323
Fragments arranged by size:
2323 1030 879 184 131 60 52 44
BbeI G_GCGC'C
Cuts at: 2137 2385 2137
Size: 248 4455
BbsI GAAGACnn'nnnn_
Cuts at: 1526 1526
Size: 4703
BciVI GTATCCnnnnn_n'
Cuts at: 818 2601 3362 4703 818
Size: 1783 761 1341 818
Fragments arranged by size:
1783 1341 818 761
BclI T'GATC_A
Cuts at: 203 2223 203
Size: 2020 2683
BglII A'GATC_T
Cuts at: 2218 4255 2218
Size: 2037 2666
BlpI GC'TnA_GC
Cuts at: 197 1012 1342 1815 197
Size: 815 330 473 3085
Fragments arranged by size:
3085 815 473 330
Bme1580I G_kGCm'C
Cuts at: 463 697 707 746 1082 2328 2421 3477 463
Size: 234 10 39 336 1246 93 1056 1689
Fragments arranged by size:
1689 1246 1056 336 234 93 39 10
BmgBI CAC'GTC
Cuts at: 495 1277 495
Size: 782 3921
BmrI ACTGGGnnnn_n'
Cuts at: 361 1238 1915 1996 2331 4475 361
Size: 877 677 81 335 2144 589
Fragments arranged by size:
2144 877 677 589 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 420 3070 420
Size: 2650 2053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 505 534 655 876 973 202
Size: 303 29 121 221 97 3932
Fragments arranged by size:
3932 303 221 121 97 29
Bpu10I CC'TnA_GC
Cuts at: 1215 1215
Size: 4703
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1879 2758 3250 3548 1072
Size: 807 879 492 298 2227
Fragments arranged by size:
2227 879 807 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4407 4407
Size: 4703
BsaAI yAC'GTr
Cuts at: 2056 2056
Size: 4703
BsaBI GATnn'nnATC
Cuts at: 6 2217 4254 6
Size: 2211 2037 455
Fragments arranged by size:
2211 2037 455
BsaHI Gr'CG_yC
Cuts at: 2134 2382 3084 4580 2134
Size: 248 702 1496 2257
Fragments arranged by size:
2257 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1157 1607 1892 2413 3091 3365 3512 3923 1157
Size: 450 285 521 678 274 147 411 1937
Fragments arranged by size:
1937 678 521 450 411 285 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 214 244 496 526 1637 1667 214
Size: 30 252 30 1111 30 3250
Fragments arranged by size:
3250 1111 252 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 537 713 537
Size: 176 4527
BseYI C'CCAG_C
Cuts at: 599 605 628 1290 1360 2128 2886 3463 3959
Size: 6 23 662 70 768 758 577 496
Cuts at: 3959 599
Size: 1343
Fragments arranged by size:
1343 768 758 662 577 496 70 23
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 910
Size: 4703
BsiEI CG_ry'CG
Cuts at: 104 2245 2291 3499 104
Size: 2141 46 1208 1308
Fragments arranged by size:
2141 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 419 671 1717 2495 2685 3477 419
Size: 252 1046 778 190 792 1645
Fragments arranged by size:
1645 1046 792 778 252 190
Bsp1286I G_dGCh'C
Cuts at: 367 419 463 647 671 697 707 746 1082
Size: 52 44 184 24 26 10 39 336
Cuts at: 1082 1586 1717 2328 2421 2495 2685 2747 3477
Size: 504 131 611 93 74 190 62 730
Cuts at: 3477 367
Size: 1593
Fragments arranged by size:
1593 730 611 504 336 190 184 131
93 74 62 52 44 39 26 24
10
BspEI T'CCGG_A
Cuts at: 3923 3923
Size: 4703
BspHI T'CATG_A
Cuts at: 446 3879 446
Size: 3433 1270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 125 381 1753 1967 2269 2650 125
Size: 256 1372 214 302 381 2178
Fragments arranged by size:
2178 1372 381 302 256 214
BsrBI CCG'CTC
Cuts at: 50 583 2995 3049 4533 4698 50
Size: 533 2412 54 1484 165 55
Fragments arranged by size:
2412 1484 533 165 55 54
BsrDI GCAATG_nn'
Cuts at: 438 2615 438
Size: 2177 2526
BsrFI r'CCGG_y
Cuts at: 2701 2882 3091 2701
Size: 181 209 4313
Fragments arranged by size:
4313 209 181
BssSI C'ACGA_G
Cuts at: 2974 3332 2974
Size: 358 4345
BstAPI GCAn_nnn'nTGC
Cuts at: 2080 2080
Size: 4703
BstBI TT'CG_AA
Cuts at: 3064 3064
Size: 4703
BstEII G'GTnAC_C
Cuts at: 258 258
Size: 4703
BstXI CCAn_nnnn'nTGG
Cuts at: 240 798 1251 240
Size: 558 453 3692
Fragments arranged by size:
3692 558 453
BstYI r'GATC_y
Cuts at: 1 623 1059 1728 2195 2218 2553 2799 3811
Size: 622 436 669 467 23 335 246 1012
Cuts at: 3811 3897 3909 4255 1
Size: 86 12 346 449
Fragments arranged by size:
1012 669 622 467 449 436 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 619 995 1588 619
Size: 376 593 3734
Fragments arranged by size:
3734 593 376
BtgI C'CryG_G
Cuts at: 107 137 476 1568 107
Size: 30 339 1092 3242
Fragments arranged by size:
3242 1092 339 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2653 2834 2997 2653
Size: 181 163 4359
Fragments arranged by size:
4359 181 163
BtsI GCAGTG_nn'
Cuts at: 1326 1946 1326
Size: 620 4083
DraI TTT'AAA
Cuts at: 936 1702 936
Size: 766 3937
DraIII CAC_nnn'GTG
Cuts at: 1227 1227
Size: 4703
DrdI GACnn_nn'nnGTC
Cuts at: 2409 3267 2409
Size: 858 3845
EaeI y'GGCC_r
Cuts at: 329 1415 1676 2288 2462 2853 2880 4225 329
Size: 1086 261 612 174 391 27 1345 807
Fragments arranged by size:
1345 1086 807 612 391 261 174 27
EagI C'GGCC_G
Cuts at: 2288 2288
Size: 4703
EarI CTCTTCn'nnn_
Cuts at: 577 1556 2726 2936 577
Size: 979 1170 210 2344
Fragments arranged by size:
2344 1170 979 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2911 3221 3367 4513 2911
Size: 310 146 1146 3101
Fragments arranged by size:
3101 1146 310 146
EcoICRI GAG'CTC
Cuts at: 417 1715 417
Size: 1298 3405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 340 505 534 655 876 973 991 1516
Size: 138 165 29 121 221 97 18 525
Cuts at: 1516 2528 2960 3707 3975 202
Size: 1012 432 747 268 930
Fragments arranged by size:
1012 930 747 525 432 268 221 165
138 121 97 29 18
EcoNI CCTnn'n_nnAGG
Cuts at: 529 574 529
Size: 45 4658
EcoO109I rG'GnC_Cy
Cuts at: 252 459 460 537 588 704 1053 1287 1842
Size: 207 1 77 51 116 349 234 555
Cuts at: 1842 252
Size: 3113
Fragments arranged by size:
3113 555 349 234 207 116 77 51
1
EcoRI G'AATT_C
Cuts at: 1707 1707
Size: 4703
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1020 2722 1020
Size: 1702 3001
FspI TGC'GCA
Cuts at: 2484 2484
Size: 4703
HaeII r_GCGC'y
Cuts at: 2137 2385 3407 4386 2137
Size: 248 1022 979 2454
Fragments arranged by size:
2454 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 1290 1322 2545 2575 2577 2607 106
Size: 32 1152 32 1223 30 2 30 2202
Fragments arranged by size:
2202 1223 1152 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1742 1742
Size: 4703
KasI G'GCGC_C
Cuts at: 2133 2381 2133
Size: 248 4455
KpnI G_GTAC'C
Cuts at: 1723 1723
Size: 4703
MluI A'CGCG_T
Cuts at: 4136 4136
Size: 4703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3374 3558 3374
Size: 184 4519
MscI TGG'CCA
Cuts at: 1678 2464 1678
Size: 786 3917
MslI CAynn'nnrTG
Cuts at: 142 385 391 859 1033 1228 1393 2692 2819
Size: 243 6 468 174 195 165 1299 127
Cuts at: 2819 142
Size: 2026
Fragments arranged by size:
2026 1299 468 243 195 174 165 127
6
MspA1I CmG'CkG
Cuts at: 201 1364 1814 2128 2488 3501 3746 201
Size: 1163 450 314 360 1013 245 1158
Fragments arranged by size:
1163 1158 1013 450 360 314 245
NaeI GCC'GGC
Cuts at: 2884 2884
Size: 4703
NarI GG'CG_CC
Cuts at: 2134 2382 2134
Size: 248 4455
NcoI C'CATG_G
Cuts at: 107 137 1568 107
Size: 30 1431 3242
Fragments arranged by size:
3242 1431 30
NgoMIV G'CCGG_C
Cuts at: 2882 2882
Size: 4703
NspI r_CATG'y
Cuts at: 390 1653 1756 2256 3163 390
Size: 1263 103 500 907 1930
Fragments arranged by size:
1930 1263 907 500 103
PasI CC'CwG_GG
Cuts at: 456 690 456
Size: 234 4469
PciI A'CATG_T
Cuts at: 3159 3159
Size: 4703
PflMI CCAn_nnn'nTGG
Cuts at: 612 612
Size: 4703
PfoI T'CCnGG_A
Cuts at: 611 1118 611
Size: 507 4196
PmeI GTTT'AAAC
Cuts at: 1702 1702
Size: 4703
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 214 246 3842 3874 214
Size: 32 3596 32 1043
Fragments arranged by size:
3596 1043 32 32
PpuMI rG'GwC_Cy
Cuts at: 588 588
Size: 4703
PspOMI G'GGCC_C
Cuts at: 459 703 459
Size: 244 4459
PstI C_TGCA'G
Cuts at: 122 1750 2435 122
Size: 1628 685 2390
Fragments arranged by size:
2390 1628 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4703
PvuII CAG'CTG
Cuts at: 201 2128 2488 201
Size: 1927 360 2416
Fragments arranged by size:
2416 1927 360
RsrII CG'GwC_CG
Cuts at: 2898 2898
Size: 4703
SacI G_AGCT'C
Cuts at: 419 1717 419
Size: 1298 3405
SalI G'TCGA_C
Cuts at: 1740 1740
Size: 4703
SapI GCTCTTCn'nnn_
Cuts at: 2726 2936 2726
Size: 210 4493
SbfI CC_TGCA'GG
Cuts at: 1750 1750
Size: 4703
ScaI AGT'ACT
Cuts at: 1003 4220 1003
Size: 3217 1486
SexAI A'CCwGG_T
Cuts at: 239 239
Size: 4703
SfcI C'TryA_G
Cuts at: 33 118 1746 2431 3424 3615 33
Size: 85 1628 685 993 191 1121
Fragments arranged by size:
1628 1121 993 685 191 85
SfoI GGC'GCC
Cuts at: 2135 2383 2135
Size: 248 4455
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
SmaI CCC'GGG
Cuts at: 1725 1725
Size: 4703
SmlI C'TyrA_G
Cuts at: 1087 1858 2773 3265 3527 1087
Size: 771 915 492 262 2263
Fragments arranged by size:
2263 915 771 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4703
SphI G_CATG'C
Cuts at: 1756 2256 1756
Size: 500 4203
StuI AGG'CCT
Cuts at: 217 1478 217
Size: 1261 3442
StyI C'CwwG_G
Cuts at: 107 137 164 1418 1554 1568 1837 2191 107
Size: 30 27 1254 136 14 269 354 2619
Fragments arranged by size:
2619 1254 354 269 136 30 27 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 469 958 2286 2419 2878 3087 469
Size: 489 1328 133 459 209 2085
Fragments arranged by size:
2085 1328 489 459 209 133
TatI w'GTAC_w
Cuts at: 1001 4218 1001
Size: 3217 1486
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 496 528 1147 1179 1671 1703 4220 4252 496
Size: 32 619 32 492 32 2517 32 947
Fragments arranged by size:
2517 947 619 492 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2500 2500
Size: 4703
XbaI T'CTAG_A
Cuts at: 1734 1734
Size: 4703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2198 2198
Size: 4703
XmaI C'CCGG_G
Cuts at: 1723 1723
Size: 4703
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI