Restriction map for
pF1KB7172
(Circular) (Six-base) MAPSORT of: pF1KB7172.seq Check: 4720 from: 1 to: 4586
pF1KB7172 4586 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 923 1624 923
Size: 701 3885
Acc65I G'GTAC_C
Cuts at: 1602 1602
Size: 4586
AclI AA'CG_TT
Cuts at: 4426 4426
Size: 4586
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 2411 2843 3590 3858 275
Size: 2136 432 747 268 1003
Fragments arranged by size:
2136 1003 747 432 268
AflIII A'CryG_T
Cuts at: 1938 3042 4019 1938
Size: 1104 977 2505
Fragments arranged by size:
2505 1104 977
AgeI A'CCGG_T
Cuts at: 2974 2974
Size: 4586
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 223 223
Size: 4586
AlwNI CAG_nnn'CTG
Cuts at: 1891 3458 1891
Size: 1567 3019
ApaLI G'TGCA_C
Cuts at: 3356 3356
Size: 4586
ApoI r'AATT_y
Cuts at: 348 853 1004 1590 3939 4195 348
Size: 505 151 586 2349 256 739
Fragments arranged by size:
2349 739 586 505 256 151
AseI AT'TA_AT
Cuts at: 20 20
Size: 4586
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
AvaI C'yCGr_G
Cuts at: 1606 1606
Size: 4586
BamHI G'GATC_C
Cuts at: 1611 1611
Size: 4586
BanI G'GyrC_C
Cuts at: 1048 1602 2016 2264 2299 1048
Size: 554 414 248 35 3335
Fragments arranged by size:
3335 554 414 248 35
BanII G_rGCy'C
Cuts at: 440 1600 2630 440
Size: 1160 1030 2396
Fragments arranged by size:
2396 1160 1030
BbeI G_GCGC'C
Cuts at: 2020 2268 2020
Size: 248 4338
BbsI GAAGACnn'nnnn_
Cuts at: 1535 1535
Size: 4586
BciVI GTATCCnnnnn_n'
Cuts at: 2484 3245 4586 2484
Size: 761 1341 2484
Fragments arranged by size:
2484 1341 761
BclI T'GATC_A
Cuts at: 2106 2106
Size: 4586
BfrBI ATG'CAT
Cuts at: 1277 1277
Size: 4586
BglI GCCn_nnn'nGGC
Cuts at: 916 916
Size: 4586
BglII A'GATC_T
Cuts at: 2101 4138 2101
Size: 2037 2549
BlpI GC'TnA_GC
Cuts at: 659 1698 659
Size: 1039 3547
Bme1580I G_kGCm'C
Cuts at: 2211 2304 3360 2211
Size: 93 1056 3437
Fragments arranged by size:
3437 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 238 432 513 1798 1879 2214 4358 238
Size: 194 81 1285 81 335 2144 466
Fragments arranged by size:
2144 1285 466 335 194 81 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 4586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 595 289
Size: 306 4280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 702 1762 2641 3133 3431 702
Size: 1060 879 492 298 1857
Fragments arranged by size:
1857 1060 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1046 4290 1046
Size: 3244 1342
BsaAI yAC'GTr
Cuts at: 1939 1939
Size: 4586
BsaBI GATnn'nnATC
Cuts at: 6 320 2100 4137 6
Size: 314 1780 2037 455
Fragments arranged by size:
2037 1780 455 314
BsaHI Gr'CG_yC
Cuts at: 2017 2265 2967 4463 2017
Size: 248 702 1496 2140
Fragments arranged by size:
2140 1496 702 248
BsaWI w'CCGG_w
Cuts at: 608 765 1775 2296 2974 3248 3395 3806 608
Size: 157 1010 521 678 274 147 411 1388
Fragments arranged by size:
1388 1010 678 521 411 274 157 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 433 775 805 1540 1570 373
Size: 30 30 342 30 735 30 3389
Fragments arranged by size:
3389 735 342 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 397 528 666 397
Size: 131 138 4317
Fragments arranged by size:
4317 138 131
BseYI C'CCAG_C
Cuts at: 2011 2769 3346 3842 2011
Size: 758 577 496 2755
Fragments arranged by size:
2755 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1398 1398
Size: 4586
BsiEI CG_ry'CG
Cuts at: 104 2128 2174 3382 104
Size: 2024 46 1208 1308
Fragments arranged by size:
2024 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 440 1600 2378 2568 3360 440
Size: 1160 778 190 792 1666
Fragments arranged by size:
1666 1160 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 560 560
Size: 4586
Bsp1286I G_dGCh'C
Cuts at: 440 1600 2211 2304 2378 2568 2630 3360 440
Size: 1160 611 93 74 190 62 730 1666
Fragments arranged by size:
1666 1160 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 608 3806 608
Size: 3198 1388
BspHI T'CATG_A
Cuts at: 1404 3762 1404
Size: 2358 2228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1636 1850 2152 2533 1636
Size: 214 302 381 3689
Fragments arranged by size:
3689 381 302 214
BsrBI CCG'CTC
Cuts at: 50 838 2878 2932 4416 4581 50
Size: 788 2040 54 1484 165 55
Fragments arranged by size:
2040 1484 788 165 55 54
BsrDI GCAATG_nn'
Cuts at: 794 2498 794
Size: 1704 2882
BsrFI r'CCGG_y
Cuts at: 2584 2765 2974 2584
Size: 181 209 4196
Fragments arranged by size:
4196 209 181
BssSI C'ACGA_G
Cuts at: 493 2857 3215 493
Size: 2364 358 1864
Fragments arranged by size:
2364 1864 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1963 1963
Size: 4586
BstBI TT'CG_AA
Cuts at: 2947 2947
Size: 4586
BstEII G'GTnAC_C
Cuts at: 1242 1242
Size: 4586
BstXI CCAn_nnnn'nTGG
Cuts at: 136 1078 1560 136
Size: 942 482 3162
Fragments arranged by size:
3162 942 482
BstYI r'GATC_y
Cuts at: 1 977 1611 2078 2101 2436 2682 3694 3780
Size: 976 634 467 23 335 246 1012 86
Cuts at: 3780 3792 4138 1
Size: 12 346 449
Fragments arranged by size:
1012 976 634 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1103 1139 107
Size: 996 36 3554
Fragments arranged by size:
3554 996 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 115 1248 2536 2717 2880 115
Size: 1133 1288 181 163 1821
Fragments arranged by size:
1821 1288 1133 181 163
BtsI GCAGTG_nn'
Cuts at: 589 1386 1829 589
Size: 797 443 3346
Fragments arranged by size:
3346 797 443
DraI TTT'AAA
Cuts at: 1585 1585
Size: 4586
DrdI GACnn_nn'nnGTC
Cuts at: 2292 3150 2292
Size: 858 3728
EaeI y'GGCC_r
Cuts at: 293 707 2171 2345 2736 2763 4108 293
Size: 414 1464 174 391 27 1345 771
Fragments arranged by size:
1464 1345 771 414 391 174 27
EagI C'GGCC_G
Cuts at: 2171 2171
Size: 4586
EarI CTCTTCn'nnn_
Cuts at: 251 445 542 932 1099 1479 2609 2819 251
Size: 194 97 390 167 380 1130 210 2018
Fragments arranged by size:
2018 1130 390 380 210 194 167 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2794 3104 3250 4396 2794
Size: 310 146 1146 2984
Fragments arranged by size:
2984 1146 310 146
EcoICRI GAG'CTC
Cuts at: 438 1598 438
Size: 1160 3426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 289 595 2411 2843 3590 3858 275
Size: 14 306 1816 432 747 268 1003
Fragments arranged by size:
1816 1003 747 432 306 268 14
EcoO109I rG'GnC_Cy
Cuts at: 1725 1725
Size: 4586
EcoRI G'AATT_C
Cuts at: 1004 1590 1004
Size: 586 4000
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1099 2605 1099
Size: 1506 3080
FspI TGC'GCA
Cuts at: 2367 2367
Size: 4586
HaeII r_GCGC'y
Cuts at: 2020 2268 3290 4269 2020
Size: 248 1022 979 2337
Fragments arranged by size:
2337 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 770 802 874 906 1144 1176 1489 1521 2428
Size: 32 72 32 238 32 313 32 907
Cuts at: 2428 2458 2460 2490 770
Size: 30 2 30 2866
Fragments arranged by size:
2866 907 313 238 72 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 159 192 1625 159
Size: 33 1433 3120
Fragments arranged by size:
3120 1433 33
HindIII A'AGCT_T
Cuts at: 661 1012 661
Size: 351 4235
KasI G'GCGC_C
Cuts at: 2016 2264 2016
Size: 248 4338
KpnI G_GTAC'C
Cuts at: 1606 1606
Size: 4586
MluI A'CGCG_T
Cuts at: 4019 4019
Size: 4586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 710 3257 3441 710
Size: 2547 184 1855
Fragments arranged by size:
2547 1855 184
MscI TGG'CCA
Cuts at: 709 2347 709
Size: 1638 2948
MslI CAynn'nnrTG
Cuts at: 1207 1294 1366 1489 2575 2702 1207
Size: 87 72 123 1086 127 3091
Fragments arranged by size:
3091 1086 127 123 87 72
MspA1I CmG'CkG
Cuts at: 1446 1697 2011 2371 3384 3629 1446
Size: 251 314 360 1013 245 2403
Fragments arranged by size:
2403 1013 360 314 251 245
NaeI GCC'GGC
Cuts at: 2767 2767
Size: 4586
NarI GG'CG_CC
Cuts at: 2017 2265 2017
Size: 248 4338
NcoI C'CATG_G
Cuts at: 107 107
Size: 4586
NgoMIV G'CCGG_C
Cuts at: 2765 2765
Size: 4586
NsiI A_TGCA'T
Cuts at: 1279 1279
Size: 4586
NspI r_CATG'y
Cuts at: 376 729 1277 1639 2139 3046 376
Size: 353 548 362 500 907 1916
Fragments arranged by size:
1916 907 548 500 362 353
PciI A'CATG_T
Cuts at: 3042 3042
Size: 4586
PflMI CCAn_nnn'nTGG
Cuts at: 1187 1187
Size: 4586
PmeI GTTT'AAAC
Cuts at: 1585 1585
Size: 4586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 3725
Size: 32 4554
PshAI GACnn'nnGTC
Cuts at: 1038 1038
Size: 4586
PstI C_TGCA'G
Cuts at: 460 1633 2318 460
Size: 1173 685 2728
Fragments arranged by size:
2728 1173 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4586
PvuII CAG'CTG
Cuts at: 2011 2371 2011
Size: 360 4226
RsrII CG'GwC_CG
Cuts at: 2781 2781
Size: 4586
SacI G_AGCT'C
Cuts at: 440 1600 440
Size: 1160 3426
SalI G'TCGA_C
Cuts at: 1623 1623
Size: 4586
SapI GCTCTTCn'nnn_
Cuts at: 445 2609 2819 445
Size: 2164 210 2212
Fragments arranged by size:
2212 2164 210
SbfI CC_TGCA'GG
Cuts at: 1633 1633
Size: 4586
ScaI AGT'ACT
Cuts at: 421 1342 4103 421
Size: 921 2761 904
Fragments arranged by size:
2761 921 904
SfcI C'TryA_G
Cuts at: 33 456 920 1478 1629 2314 3307 3498 33
Size: 423 464 558 151 685 993 191 1121
Fragments arranged by size:
1121 993 685 558 464 423 191 151
SfoI GGC'GCC
Cuts at: 2018 2266 2018
Size: 248 4338
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
SmaI CCC'GGG
Cuts at: 1608 1608
Size: 4586
SmlI C'TyrA_G
Cuts at: 681 1741 2656 3148 3410 681
Size: 1060 915 492 262 1857
Fragments arranged by size:
1857 1060 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4586
SphI G_CATG'C
Cuts at: 1639 2139 1639
Size: 500 4086
StyI C'CwwG_G
Cuts at: 107 929 1247 1559 1720 2074 107
Size: 822 318 312 161 354 2619
Fragments arranged by size:
2619 822 354 318 312 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1028 1249 2169 2302 2761 2970 1028
Size: 221 920 133 459 209 2644
Fragments arranged by size:
2644 920 459 221 209 133
TatI w'GTAC_w
Cuts at: 419 1340 4101 419
Size: 921 2761 904
Fragments arranged by size:
2761 921 904
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 373 405 775 807 4103 4135 373
Size: 32 370 32 3296 32 824
Fragments arranged by size:
3296 824 370 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 886 2383 886
Size: 1497 3089
XbaI T'CTAG_A
Cuts at: 1617 1617
Size: 4586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 745 2081 745
Size: 1336 3250
XmaI C'CCGG_G
Cuts at: 1606 1606
Size: 4586
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I
BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI PacI PasI PfoI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI