Restriction map for
pF1KB7168
(Circular) (Six-base) MAPSORT of: pF1KB7168.seq Check: 3920 from: 1 to: 4349
pF1KB7168 4349 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 929 1387 929
Size: 458 3891
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 705 2174 2606 3353 3621 705
Size: 1469 432 747 268 1433
Fragments arranged by size:
1469 1433 747 432 268
AflIII A'CryG_T
Cuts at: 1701 2805 3782 1701
Size: 1104 977 2268
Fragments arranged by size:
2268 1104 977
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1022 1054 1022
Size: 32 4317
AlwNI CAG_nnn'CTG
Cuts at: 1654 3221 1654
Size: 1567 2782
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 246 1129 1296 1353 3702 3958 246
Size: 883 167 57 2349 256 637
Fragments arranged by size:
2349 883 637 256 167 57
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 1369 1369
Size: 4349
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 1365 1779 2027 2062 1365
Size: 414 248 35 3652
Fragments arranged by size:
3652 414 248 35
BanII G_rGCy'C
Cuts at: 1363 2393 1363
Size: 1030 3319
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BbsI GAAGACnn'nnnn_
Cuts at: 353 711 845 353
Size: 358 134 3857
Fragments arranged by size:
3857 358 134
BciVI GTATCCnnnnn_n'
Cuts at: 105 318 589 2247 3008 4349 105
Size: 213 271 1658 761 1341 105
Fragments arranged by size:
1658 1341 761 271 213 105
BclI T'GATC_A
Cuts at: 356 1869 356
Size: 1513 2836
BglI GCCn_nnn'nGGC
Cuts at: 733 733
Size: 4349
BglII A'GATC_T
Cuts at: 1864 3901 1864
Size: 2037 2312
BlpI GC'TnA_GC
Cuts at: 1461 1461
Size: 4349
Bme1580I G_kGCm'C
Cuts at: 1974 2067 3123 1974
Size: 93 1056 3200
Fragments arranged by size:
3200 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 584 998 1561 1642 1977 4121 584
Size: 414 563 81 335 2144 812
Fragments arranged by size:
2144 812 563 414 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 4349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 1080 1311 108
Size: 972 231 3146
Fragments arranged by size:
3146 972 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1525 2404 2896 3194 1525
Size: 879 492 298 2680
Fragments arranged by size:
2680 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4053 4053
Size: 4349
BsaAI yAC'GTr
Cuts at: 1702 1702
Size: 4349
BsaBI GATnn'nnATC
Cuts at: 6 520 1863 3900 6
Size: 514 1343 2037 455
Fragments arranged by size:
2037 1343 514 455
BsaHI Gr'CG_yC
Cuts at: 1780 2028 2730 4226 1780
Size: 248 702 1496 1903
Fragments arranged by size:
1903 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1538 2059 2737 3011 3158 3569 1538
Size: 521 678 274 147 411 2318
Fragments arranged by size:
2318 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 318 348 318
Size: 30 4319
BseYI C'CCAG_C
Cuts at: 200 679 1774 2532 3109 3605 200
Size: 479 1095 758 577 496 944
Fragments arranged by size:
1095 944 758 577 496 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 950 1189 950
Size: 239 4110
BsiEI CG_ry'CG
Cuts at: 104 1891 1937 3145 104
Size: 1787 46 1208 1308
Fragments arranged by size:
1787 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1363 2141 2331 3123 1363
Size: 778 190 792 2589
Fragments arranged by size:
2589 792 778 190
BsmI GAATG_Cn'
Cuts at: 1099 1099
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 1363 1974 2067 2141 2331 2393 3123 1363
Size: 611 93 74 190 62 730 2589
Fragments arranged by size:
2589 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 815 3525 815
Size: 2710 1639
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1399 1613 1915 2296 1399
Size: 214 302 381 3452
Fragments arranged by size:
3452 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2641 2695 4179 4344 50
Size: 2591 54 1484 165 55
Fragments arranged by size:
2591 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 636 690 1070 2261 636
Size: 54 380 1191 2724
Fragments arranged by size:
2724 1191 380 54
BsrFI r'CCGG_y
Cuts at: 2347 2528 2737 2347
Size: 181 209 3959
Fragments arranged by size:
3959 209 181
BssSI C'ACGA_G
Cuts at: 1198 2620 2978 1198
Size: 1422 358 2569
Fragments arranged by size:
2569 1422 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1726 1726
Size: 4349
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstYI r'GATC_y
Cuts at: 1 436 934 1374 1841 1864 2199 2445 3457
Size: 435 498 440 467 23 335 246 1012
Cuts at: 3457 3543 3555 3901 1
Size: 86 12 346 449
Fragments arranged by size:
1012 498 467 449 440 435 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 930 930
Size: 4349
BtgI C'CryG_G
Cuts at: 107 320 107
Size: 213 4136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 224 2299 2480 2643 224
Size: 2075 181 163 1930
Fragments arranged by size:
2075 1930 181 163
BtsI GCAGTG_nn'
Cuts at: 908 1592 908
Size: 684 3665
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DraIII CAC_nnn'GTG
Cuts at: 698 698
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 1934 2108 2499 2526 3871 1934
Size: 174 391 27 1345 2412
Fragments arranged by size:
2412 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1934 1934
Size: 4349
EarI CTCTTCn'nnn_
Cuts at: 616 2372 2582 616
Size: 1756 210 2383
Fragments arranged by size:
2383 1756 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2557 2867 3013 4159 2557
Size: 310 146 1146 2747
Fragments arranged by size:
2747 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1361 1361
Size: 4349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 705 1080 1311 2174 2606 3353 3621 108
Size: 597 375 231 863 432 747 268 836
Fragments arranged by size:
863 836 747 597 432 375 268 231
EcoO109I rG'GnC_Cy
Cuts at: 576 1488 576
Size: 912 3437
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4349
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 1783 2031 3053 4032 1783
Size: 248 1022 979 2100
Fragments arranged by size:
2100 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 593 625 897 929 1229 1261 2191 2221 2223
Size: 32 272 32 300 32 930 30 2
Cuts at: 2223 2253 593
Size: 30 2689
Fragments arranged by size:
2689 930 300 272 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 771 1388 771
Size: 617 3732
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 964 3020 3204 964
Size: 2056 184 2109
Fragments arranged by size:
2109 2056 184
MscI TGG'CCA
Cuts at: 2110 2110
Size: 4349
MslI CAynn'nnrTG
Cuts at: 172 455 889 2338 2465 172
Size: 283 434 1449 127 2056
Fragments arranged by size:
2056 1449 434 283 127
MspA1I CmG'CkG
Cuts at: 1460 1774 2134 3147 3392 1460
Size: 314 360 1013 245 2417
Fragments arranged by size:
2417 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 4349
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NcoI C'CATG_G
Cuts at: 107 320 107
Size: 213 4136
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 4349
NspI r_CATG'y
Cuts at: 960 1402 1902 2809 960
Size: 442 500 907 2500
Fragments arranged by size:
2500 907 500 442
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PflMI CCAn_nnn'nTGG
Cuts at: 698 1048 698
Size: 350 3999
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 309 341 309
Size: 32 4317
PstI C_TGCA'G
Cuts at: 1098 1396 2081 1098
Size: 298 685 3366
Fragments arranged by size:
3366 685 298
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 1774 2134 1774
Size: 360 3989
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 1363 1363
Size: 4349
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SfcI C'TryA_G
Cuts at: 33 713 779 1094 1392 2077 3070 3261 33
Size: 680 66 315 298 685 993 191 1121
Fragments arranged by size:
1121 993 685 680 315 298 191 66
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 1371 1371
Size: 4349
SmlI C'TyrA_G
Cuts at: 1504 2419 2911 3173 1504
Size: 915 492 262 2680
Fragments arranged by size:
2680 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StyI C'CwwG_G
Cuts at: 107 320 1121 1483 1837 107
Size: 213 801 362 354 2619
Fragments arranged by size:
2619 801 362 354 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 479 1932 2065 2524 2733 479
Size: 1453 133 459 209 2095
Fragments arranged by size:
2095 1453 459 209 133
TatI w'GTAC_w
Cuts at: 1155 3864 1155
Size: 2709 1640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4317
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 507 1380 507
Size: 873 3476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 171 891 1844 171
Size: 720 953 2676
Fragments arranged by size:
2676 953 720
XmaI C'CCGG_G
Cuts at: 1369 1369
Size: 4349
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BseRI BsiWI BsmBI BsrGI BssHII BstEII BstXI
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI