Restriction map for
pF1KB7163
(Circular) (Six-base) MAPSORT of: pF1KB7163.seq Check: 4323 from: 1 to: 4277
pF1KB7163 4277 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 979 1315 979
Size: 336 3941
Acc65I G'GTAC_C
Cuts at: 1293 1293
Size: 4277
AclI AA'CG_TT
Cuts at: 4117 4117
Size: 4277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 624 703 808 846 1004 1153 2102 2534
Size: 441 79 105 38 158 149 949 432
Cuts at: 2534 3281 3549 183
Size: 747 268 911
Fragments arranged by size:
949 911 747 441 432 268 158 149
105 79 38
AflIII A'CryG_T
Cuts at: 1629 2733 3710 1629
Size: 1104 977 2196
Fragments arranged by size:
2196 1104 977
AgeI A'CCGG_T
Cuts at: 721 2665 721
Size: 1944 2333
AleI CACnn'nnGTG
Cuts at: 724 724
Size: 4277
AlwNI CAG_nnn'CTG
Cuts at: 1582 3149 1582
Size: 1567 2710
ApaLI G'TGCA_C
Cuts at: 3047 3047
Size: 4277
ApoI r'AATT_y
Cuts at: 421 886 1281 3630 3886 421
Size: 465 395 2349 256 812
Fragments arranged by size:
2349 812 465 395 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4277
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
AvaI C'yCGr_G
Cuts at: 1297 1297
Size: 4277
BamHI G'GATC_C
Cuts at: 1302 1302
Size: 4277
BanI G'GyrC_C
Cuts at: 1212 1293 1707 1955 1990 1212
Size: 81 414 248 35 3499
Fragments arranged by size:
3499 414 248 81 35
BanII G_rGCy'C
Cuts at: 140 254 311 1291 2321 140
Size: 114 57 980 1030 2096
Fragments arranged by size:
2096 1030 980 114 57
BbeI G_GCGC'C
Cuts at: 1711 1959 1711
Size: 248 4029
BbsI GAAGACnn'nnnn_
Cuts at: 796 796
Size: 4277
BciVI GTATCCnnnnn_n'
Cuts at: 2175 2936 4277 2175
Size: 761 1341 2175
Fragments arranged by size:
2175 1341 761
BclI T'GATC_A
Cuts at: 1797 1797
Size: 4277
BglII A'GATC_T
Cuts at: 1792 3829 1792
Size: 2037 2240
BlpI GC'TnA_GC
Cuts at: 1389 1389
Size: 4277
Bme1580I G_kGCm'C
Cuts at: 215 1902 1995 3051 215
Size: 1687 93 1056 1441
Fragments arranged by size:
1687 1441 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 209 616 964 1017 1489 1570 1905 4049 209
Size: 407 348 53 472 81 335 2144 437
Fragments arranged by size:
2144 472 437 407 348 335 81 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 469 2644 469
Size: 2175 2102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 781 799 238
Size: 543 18 3716
Fragments arranged by size:
3716 543 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 1453 2332 2824 3122 103
Size: 1350 879 492 298 1258
Fragments arranged by size:
1350 1258 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 199 3981 199
Size: 3782 495
BsaAI yAC'GTr
Cuts at: 1630 1630
Size: 4277
BsaBI GATnn'nnATC
Cuts at: 6 1791 3828 6
Size: 1785 2037 455
Fragments arranged by size:
2037 1785 455
BsaHI Gr'CG_yC
Cuts at: 1708 1956 2658 4154 1708
Size: 248 702 1496 1831
Fragments arranged by size:
1831 1496 702 248
BsaWI w'CCGG_w
Cuts at: 721 1466 1987 2665 2939 3086 3497 721
Size: 745 521 678 274 147 411 1501
Fragments arranged by size:
1501 745 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 653 683 653
Size: 30 4247
BseYI C'CCAG_C
Cuts at: 1702 2460 3037 3533 1702
Size: 758 577 496 2446
Fragments arranged by size:
2446 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1058 650
Size: 408 3869
BsiEI CG_ry'CG
Cuts at: 104 1100 1819 1865 3073 104
Size: 996 719 46 1208 1308
Fragments arranged by size:
1308 1208 996 719 46
BsiHKAI G_wGCw'C
Cuts at: 140 254 1291 2069 2259 3051 140
Size: 114 1037 778 190 792 1366
Fragments arranged by size:
1366 1037 792 778 190 114
Bsp1286I G_dGCh'C
Cuts at: 140 215 254 311 1291 1902 1995 2069 2259
Size: 75 39 57 980 611 93 74 190
Cuts at: 2259 2321 3051 140
Size: 62 730 1366
Fragments arranged by size:
1366 980 730 611 190 93 75 74
62 57 39
BspEI T'CCGG_A
Cuts at: 3497 3497
Size: 4277
BspHI T'CATG_A
Cuts at: 3453 3453
Size: 4277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1327 1541 1843 2224 1327
Size: 214 302 381 3380
Fragments arranged by size:
3380 381 302 214
BsrBI CCG'CTC
Cuts at: 50 664 2569 2623 4107 4272 50
Size: 614 1905 54 1484 165 55
Fragments arranged by size:
1905 1484 614 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2189 2189
Size: 4277
BsrFI r'CCGG_y
Cuts at: 721 2275 2456 2665 721
Size: 1554 181 209 2333
Fragments arranged by size:
2333 1554 209 181
BsrGI T'GTAC_A
Cuts at: 944 944
Size: 4277
BssSI C'ACGA_G
Cuts at: 149 2548 2906 149
Size: 2399 358 1520
Fragments arranged by size:
2399 1520 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1654 1654
Size: 4277
BstBI TT'CG_AA
Cuts at: 2638 2638
Size: 4277
BstXI CCAn_nnnn'nTGG
Cuts at: 726 759 1195 726
Size: 33 436 3808
Fragments arranged by size:
3808 436 33
BstYI r'GATC_y
Cuts at: 1 122 987 1302 1769 1792 2127 2373 3385
Size: 121 865 315 467 23 335 246 1012
Cuts at: 3385 3471 3483 3829 1
Size: 86 12 346 449
Fragments arranged by size:
1012 865 467 449 346 335 315 246
121 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 492 2227 2408 2571 492
Size: 1735 181 163 2198
Fragments arranged by size:
2198 1735 181 163
BtsI GCAGTG_nn'
Cuts at: 1520 1520
Size: 4277
DraI TTT'AAA
Cuts at: 1276 1276
Size: 4277
DrdI GACnn_nn'nnGTC
Cuts at: 1983 2841 1983
Size: 858 3419
EaeI y'GGCC_r
Cuts at: 596 1031 1097 1862 2036 2427 2454 3799 596
Size: 435 66 765 174 391 27 1345 1074
Fragments arranged by size:
1345 1074 765 435 391 174 66 27
EagI C'GGCC_G
Cuts at: 1097 1862 1097
Size: 765 3512
EarI CTCTTCn'nnn_
Cuts at: 184 202 2300 2510 184
Size: 18 2098 210 1951
Fragments arranged by size:
2098 1951 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2485 2795 2941 4087 2485
Size: 310 146 1146 2675
Fragments arranged by size:
2675 1146 310 146
EcoICRI GAG'CTC
Cuts at: 138 252 1289 138
Size: 114 1037 3126
Fragments arranged by size:
3126 1037 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 238 624 703 781 799 808 846 1004
Size: 55 386 79 78 18 9 38 158
Cuts at: 1004 1153 2102 2534 3281 3549 183
Size: 149 949 432 747 268 911
Fragments arranged by size:
949 911 747 432 386 268 158 149
79 78 55 38 18 9
EcoO109I rG'GnC_Cy
Cuts at: 243 1416 243
Size: 1173 3104
EcoRI G'AATT_C
Cuts at: 886 1281 886
Size: 395 3882
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 4277
FspI TGC'GCA
Cuts at: 2058 2058
Size: 4277
HaeII r_GCGC'y
Cuts at: 1711 1959 2981 3960 1711
Size: 248 1022 979 2028
Fragments arranged by size:
2028 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2119 2149 2151 2181 2119
Size: 30 2 30 4215
Fragments arranged by size:
4215 30 30 2
HincII GTy'rAC
Cuts at: 1316 1316
Size: 4277
KasI G'GCGC_C
Cuts at: 1707 1955 1707
Size: 248 4029
KpnI G_GTAC'C
Cuts at: 1297 1297
Size: 4277
MluI A'CGCG_T
Cuts at: 3710 3710
Size: 4277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2948 3132 2948
Size: 184 4093
MscI TGG'CCA
Cuts at: 598 2038 598
Size: 1440 2837
MslI CAynn'nnrTG
Cuts at: 724 2266 2393 724
Size: 1542 127 2608
Fragments arranged by size:
2608 1542 127
MspA1I CmG'CkG
Cuts at: 1388 1702 2062 3075 3320 1388
Size: 314 360 1013 245 2345
Fragments arranged by size:
2345 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2458 2458
Size: 4277
NarI GG'CG_CC
Cuts at: 1708 1956 1708
Size: 248 4029
NgoMIV G'CCGG_C
Cuts at: 2456 2456
Size: 4277
NspI r_CATG'y
Cuts at: 1330 1830 2737 1330
Size: 500 907 2870
Fragments arranged by size:
2870 907 500
PciI A'CATG_T
Cuts at: 2733 2733
Size: 4277
PflMI CCAn_nnn'nTGG
Cuts at: 1053 1199 1053
Size: 146 4131
PfoI T'CCnGG_A
Cuts at: 254 758 254
Size: 504 3773
PmeI GTTT'AAAC
Cuts at: 1276 1276
Size: 4277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 4245
PpuMI rG'GwC_Cy
Cuts at: 243 243
Size: 4277
PstI C_TGCA'G
Cuts at: 1324 2009 1324
Size: 685 3592
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4277
PvuII CAG'CTG
Cuts at: 1702 2062 1702
Size: 360 3917
RsrII CG'GwC_CG
Cuts at: 2472 2472
Size: 4277
SacI G_AGCT'C
Cuts at: 140 254 1291 140
Size: 114 1037 3126
Fragments arranged by size:
3126 1037 114
SalI G'TCGA_C
Cuts at: 1314 1314
Size: 4277
SapI GCTCTTCn'nnn_
Cuts at: 2300 2510 2300
Size: 210 4067
SbfI CC_TGCA'GG
Cuts at: 1324 1324
Size: 4277
ScaI AGT'ACT
Cuts at: 3794 3794
Size: 4277
SexAI A'CCwGG_T
Cuts at: 910 1256 910
Size: 346 3931
SfcI C'TryA_G
Cuts at: 33 1320 2005 2998 3189 33
Size: 1287 685 993 191 1121
Fragments arranged by size:
1287 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1709 1957 1709
Size: 248 4029
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
SgrAI Cr'CCGG_yG
Cuts at: 721 721
Size: 4277
SmaI CCC'GGG
Cuts at: 1299 1299
Size: 4277
SmlI C'TyrA_G
Cuts at: 118 1432 2347 2839 3101 118
Size: 1314 915 492 262 1294
Fragments arranged by size:
1314 1294 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4277
SphI G_CATG'C
Cuts at: 1330 1830 1330
Size: 500 3777
StuI AGG'CCT
Cuts at: 776 850 776
Size: 74 4203
StyI C'CwwG_G
Cuts at: 1411 1765 1411
Size: 354 3923
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1860 1993 2452 2661 1860
Size: 133 459 209 3476
Fragments arranged by size:
3476 459 209 133
TatI w'GTAC_w
Cuts at: 944 3792 944
Size: 2848 1429
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3794 3826 3794
Size: 32 4245
Tth111I GACn'n_nGTC
Cuts at: 1161 2074 1161
Size: 913 3364
XbaI T'CTAG_A
Cuts at: 1308 1308
Size: 4277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1772 1772
Size: 4277
XmaI C'CCGG_G
Cuts at: 1297 1297
Size: 4277
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI