Restriction map for
pF1KB7162
(Circular) (Six-base) MAPSORT of: pF1KB7162.seq Check: 1468 from: 1 to: 4238
pF1KB7162 4238 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1072 1072
Size: 4238
AccI GT'mk_AC
Cuts at: 1276 1276
Size: 4238
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 562 2063 2495 3242 3510 110
Size: 452 1501 432 747 268 838
Fragments arranged by size:
1501 838 747 452 432 268
AflIII A'CryG_T
Cuts at: 872 1590 2694 3671 872
Size: 718 1104 977 1439
Fragments arranged by size:
1439 1104 977 718
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AhdI GACnn_n'nnGTC
Cuts at: 396 396
Size: 4238
AlwNI CAG_nnn'CTG
Cuts at: 204 822 896 1543 3110 204
Size: 618 74 647 1567 1332
Fragments arranged by size:
1567 1332 647 618 74
ApaI G_GGCC'C
Cuts at: 1137 1137
Size: 4238
ApaLI G'TGCA_C
Cuts at: 3008 3008
Size: 4238
ApoI r'AATT_y
Cuts at: 1242 3591 3847 1242
Size: 2349 256 1633
Fragments arranged by size:
2349 1633 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 1111 1150 1258 1111
Size: 39 108 4091
Fragments arranged by size:
4091 108 39
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 787 1105 1254 1668 1916 1951 787
Size: 318 149 414 248 35 3074
Fragments arranged by size:
3074 414 318 248 149 35
BanII G_rGCy'C
Cuts at: 1137 1151 1252 2282 1137
Size: 14 101 1030 3093
Fragments arranged by size:
3093 1030 101 14
BbeI G_GCGC'C
Cuts at: 1672 1920 1672
Size: 248 3990
BciVI GTATCCnnnnn_n'
Cuts at: 2136 2897 4238 2136
Size: 761 1341 2136
Fragments arranged by size:
2136 1341 761
BclI T'GATC_A
Cuts at: 1758 1758
Size: 4238
BglI GCCn_nnn'nGGC
Cuts at: 366 1051 1090 1114 366
Size: 685 39 24 3490
Fragments arranged by size:
3490 685 39 24
BglII A'GATC_T
Cuts at: 1753 3790 1753
Size: 2037 2201
BlpI GC'TnA_GC
Cuts at: 829 1350 829
Size: 521 3717
Bme1580I G_kGCm'C
Cuts at: 1110 1137 1863 1956 3012 1110
Size: 27 726 93 1056 2336
Fragments arranged by size:
2336 1056 726 93 27
BmrI ACTGGGnnnn_n'
Cuts at: 1450 1531 1866 4010 1450
Size: 81 335 2144 1678
Fragments arranged by size:
2144 1678 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1053 1053
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 2293 2785 3083 1414
Size: 879 492 298 2569
Fragments arranged by size:
2569 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 404 1003 3942 404
Size: 599 2939 700
Fragments arranged by size:
2939 700 599
BsaAI yAC'GTr
Cuts at: 378 873 1591 378
Size: 495 718 3025
Fragments arranged by size:
3025 718 495
BsaBI GATnn'nnATC
Cuts at: 6 1752 3789 6
Size: 1746 2037 455
Fragments arranged by size:
2037 1746 455
BsaHI Gr'CG_yC
Cuts at: 557 745 1669 1917 2619 4115 557
Size: 188 924 248 702 1496 680
Fragments arranged by size:
1496 924 702 680 248 188
BsaWI w'CCGG_w
Cuts at: 533 1427 1948 2626 2900 3047 3458 533
Size: 894 521 678 274 147 411 1313
Fragments arranged by size:
1313 894 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 931 961 931
Size: 30 4208
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 153 631 1159 126
Size: 27 478 528 3205
Fragments arranged by size:
3205 528 478 27
BseYI C'CCAG_C
Cuts at: 303 1101 1663 2421 2998 3494 303
Size: 798 562 758 577 496 1047
Fragments arranged by size:
1047 798 758 577 562 496
BsiEI CG_ry'CG
Cuts at: 104 551 1080 1780 1826 3034 104
Size: 447 529 700 46 1208 1308
Fragments arranged by size:
1308 1208 700 529 447 46
BsiHKAI G_wGCw'C
Cuts at: 662 830 992 1252 2030 2220 3012 662
Size: 168 162 260 778 190 792 1888
Fragments arranged by size:
1888 792 778 260 190 168 162
Bsp1286I G_dGCh'C
Cuts at: 662 830 992 1110 1137 1151 1252 1863 1956
Size: 168 162 118 27 14 101 611 93
Cuts at: 1956 2030 2220 2282 3012 662
Size: 74 190 62 730 1888
Fragments arranged by size:
1888 730 611 190 168 162 118 101
93 74 62 27 14
BspEI T'CCGG_A
Cuts at: 533 3458 533
Size: 2925 1313
BspHI T'CATG_A
Cuts at: 3414 3414
Size: 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 287 1072 1214 1288 1502 1804 2185 287
Size: 785 142 74 214 302 381 2340
Fragments arranged by size:
2340 785 381 302 214 142 74
BsrBI CCG'CTC
Cuts at: 50 2530 2584 4068 4233 50
Size: 2480 54 1484 165 55
Fragments arranged by size:
2480 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2150 2150
Size: 4238
BsrFI r'CCGG_y
Cuts at: 1075 2236 2417 2626 1075
Size: 1161 181 209 2687
Fragments arranged by size:
2687 1161 209 181
BssHII G'CGCG_C
Cuts at: 684 765 684
Size: 81 4157
BssSI C'ACGA_G
Cuts at: 613 2509 2867 613
Size: 1896 358 1984
Fragments arranged by size:
1984 1896 358
BstAPI GCAn_nnn'nTGC
Cuts at: 378 1615 378
Size: 1237 3001
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstYI r'GATC_y
Cuts at: 1 812 1263 1730 1753 2088 2334 3346 3432
Size: 811 451 467 23 335 246 1012 86
Cuts at: 3432 3444 3790 1
Size: 12 346 449
Fragments arranged by size:
1012 811 467 451 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 485 783 836 1038 1217 485
Size: 298 53 202 179 3506
Fragments arranged by size:
3506 298 202 179 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2188 2369 2532 2188
Size: 181 163 3894
Fragments arranged by size:
3894 181 163
BtsI GCAGTG_nn'
Cuts at: 149 1481 149
Size: 1332 2906
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DraIII CAC_nnn'GTG
Cuts at: 648 648
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 1944 2802 1944
Size: 858 3380
EaeI y'GGCC_r
Cuts at: 248 332 548 1077 1229 1823 1997 2388 2415
Size: 84 216 529 152 594 174 391 27
Cuts at: 2415 3760 248
Size: 1345 726
Fragments arranged by size:
1345 726 594 529 391 216 174 152
84 27
EagI C'GGCC_G
Cuts at: 548 1077 1823 548
Size: 529 746 2963
Fragments arranged by size:
2963 746 529
EarI CTCTTCn'nnn_
Cuts at: 346 727 790 2261 2471 346
Size: 381 63 1471 210 2113
Fragments arranged by size:
2113 1471 381 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2446 2756 2902 4048 2446
Size: 310 146 1146 2636
Fragments arranged by size:
2636 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1250 1250
Size: 4238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 562 1053 2063 2495 3242 3510 110
Size: 452 491 1010 432 747 268 838
Fragments arranged by size:
1010 838 747 491 452 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1068 1068
Size: 4238
EcoO109I rG'GnC_Cy
Cuts at: 1023 1043 1133 1377 1023
Size: 20 90 244 3884
Fragments arranged by size:
3884 244 90 20
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 4238
FspI TGC'GCA
Cuts at: 889 2019 889
Size: 1130 3108
HaeII r_GCGC'y
Cuts at: 1672 1920 2942 3921 1672
Size: 248 1022 979 1989
Fragments arranged by size:
1989 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 148 180 382 414 2080 2110 2112 2142 148
Size: 32 202 32 1666 30 2 30 2244
Fragments arranged by size:
2244 1666 202 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1160 1277 1160
Size: 117 4121
KasI G'GCGC_C
Cuts at: 1668 1916 1668
Size: 248 3990
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2909 3093 2909
Size: 184 4054
MscI TGG'CCA
Cuts at: 1999 1999
Size: 4238
MslI CAynn'nnrTG
Cuts at: 460 484 2227 2354 460
Size: 24 1743 127 2344
Fragments arranged by size:
2344 1743 127 24
MspA1I CmG'CkG
Cuts at: 819 908 1040 1101 1349 1663 2023 3036 3281
Size: 89 132 61 248 314 360 1013 245
Cuts at: 3281 819
Size: 1776
Fragments arranged by size:
1776 1013 360 314 248 245 132 89
61
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 4238
NarI GG'CG_CC
Cuts at: 1669 1917 1669
Size: 248 3990
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 4238
NspI r_CATG'y
Cuts at: 1291 1791 2698 1291
Size: 500 907 2831
Fragments arranged by size:
2831 907 500
PasI CC'CwG_GG
Cuts at: 1137 1137
Size: 4238
PciI A'CATG_T
Cuts at: 2694 2694
Size: 4238
PflMI CCAn_nnn'nTGG
Cuts at: 204 822 204
Size: 618 3620
PfoI T'CCnGG_A
Cuts at: 1069 1120 1069
Size: 51 4187
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PmlI CAC'GTG
Cuts at: 873 873
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4206
PpuMI rG'GwC_Cy
Cuts at: 1023 1023
Size: 4238
PspOMI G'GGCC_C
Cuts at: 1133 1133
Size: 4238
PstI C_TGCA'G
Cuts at: 156 284 374 386 1285 1970 156
Size: 128 90 12 899 685 2424
Fragments arranged by size:
2424 899 685 128 90 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 908 1663 2023 908
Size: 755 360 3123
Fragments arranged by size:
3123 755 360
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 1252 1252
Size: 4238
SacII CC_GC'GG
Cuts at: 1041 1041
Size: 4238
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 790 2261 2471 790
Size: 1471 210 2557
Fragments arranged by size:
2557 1471 210
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SfcI C'TryA_G
Cuts at: 33 152 280 370 382 1281 1966 2959 3150
Size: 119 128 90 12 899 685 993 191
Cuts at: 3150 33
Size: 1121
Fragments arranged by size:
1121 993 899 685 191 128 119 90
12
SfiI GGCCn_nnn'nGGCC
Cuts at: 1051 1090 1051
Size: 39 4199
SfoI GGC'GCC
Cuts at: 1670 1918 1670
Size: 248 3990
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 1260 1260
Size: 4238
SmlI C'TyrA_G
Cuts at: 1393 2308 2800 3062 1393
Size: 915 492 262 2569
Fragments arranged by size:
2569 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 1291 1791 1291
Size: 500 3738
StuI AGG'CCT
Cuts at: 1093 1117 1093
Size: 24 4214
StyI C'CwwG_G
Cuts at: 578 920 1372 1726 578
Size: 342 452 354 3090
Fragments arranged by size:
3090 452 354 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 805 1030 1093 1821 1954 2413 2622 805
Size: 225 63 728 133 459 209 2421
Fragments arranged by size:
2421 728 459 225 209 133 63
TatI w'GTAC_w
Cuts at: 3753 3753
Size: 4238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4206
Tth111I GACn'n_nGTC
Cuts at: 2035 2035
Size: 4238
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1224 1733 114
Size: 1110 509 2619
Fragments arranged by size:
2619 1110 509
XmaI C'CCGG_G
Cuts at: 1258 1258
Size: 4238
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PspXI PsrI SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI