Restriction map for
pF1KB7161
(Circular) (Six-base) MAPSORT of: pF1KB7161.seq Check: 4212 from: 1 to: 4232
pF1KB7161 4232 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1270 1270
Size: 4232
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 140 4072 140
Size: 3932 300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 2489 3236 3504 2057
Size: 432 747 268 2785
Fragments arranged by size:
2785 747 432 268
AflIII A'CryG_T
Cuts at: 1584 2688 3665 1584
Size: 1104 977 2151
Fragments arranged by size:
2151 1104 977
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AhdI GACnn_n'nnGTC
Cuts at: 507 507
Size: 4232
AleI CACnn'nnGTG
Cuts at: 722 722
Size: 4232
AlwNI CAG_nnn'CTG
Cuts at: 495 1537 3104 495
Size: 1042 1567 1623
Fragments arranged by size:
1623 1567 1042
ApaI G_GGCC'C
Cuts at: 712 712
Size: 4232
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 1000 1236 3585 3841 1000
Size: 236 2349 256 1391
Fragments arranged by size:
2349 1391 256 236
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 781 1252 781
Size: 471 3761
AvrII C'CTAG_G
Cuts at: 916 916
Size: 4232
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 318 481 972 1248 1662 1910 1945 318
Size: 163 491 276 414 248 35 2605
Fragments arranged by size:
2605 491 414 276 248 163 35
BanII G_rGCy'C
Cuts at: 412 439 663 712 926 1023 1246 2276 412
Size: 27 224 49 214 97 223 1030 2368
Fragments arranged by size:
2368 1030 224 223 214 97 49 27
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BbsI GAAGACnn'nnnn_
Cuts at: 182 182
Size: 4232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4198
BciVI GTATCCnnnnn_n'
Cuts at: 468 2130 2891 4232 468
Size: 1662 761 1341 468
Fragments arranged by size:
1662 1341 761 468
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 1344 1344
Size: 4232
Bme1580I G_kGCm'C
Cuts at: 323 712 977 1857 1950 3006 323
Size: 389 265 880 93 1056 1549
Fragments arranged by size:
1549 1056 880 389 265 93
BmrI ACTGGGnnnn_n'
Cuts at: 189 353 1444 1525 1860 4004 189
Size: 164 1091 81 335 2144 417
Fragments arranged by size:
2144 1091 417 335 164 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 240 339 2599 240
Size: 99 2260 1873
Fragments arranged by size:
2260 1873 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 979
Size: 4232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 1408 2287 2779 3077 637
Size: 771 879 492 298 1792
Fragments arranged by size:
1792 879 771 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3936 3936
Size: 4232
BsaAI yAC'GTr
Cuts at: 1585 1585
Size: 4232
BsaBI GATnn'nnATC
Cuts at: 6 619 1746 3783 6
Size: 613 1127 2037 455
Fragments arranged by size:
2037 1127 613 455
BsaHI Gr'CG_yC
Cuts at: 1663 1911 2613 4109 1663
Size: 248 702 1496 1786
Fragments arranged by size:
1786 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1421 1942 2620 2894 3041 3452 1421
Size: 521 678 274 147 411 2201
Fragments arranged by size:
2201 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 136 166 136
Size: 30 4202
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 216 229 322 325 328 411 463 556 598
Size: 13 93 3 3 83 52 93 42
Cuts at: 598 859 916 977 216
Size: 261 57 61 3471
Fragments arranged by size:
3471 261 93 93 83 61 57 52
42 13 3 3
BseYI C'CCAG_C
Cuts at: 886 1657 2415 2992 3488 886
Size: 771 758 577 496 1630
Fragments arranged by size:
1630 771 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 964
Size: 4232
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3028 104
Size: 1670 46 1208 1308
Fragments arranged by size:
1670 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 412 439 899 926 1246 2024 2214 3006 412
Size: 27 460 27 320 778 190 792 1638
Fragments arranged by size:
1638 792 778 460 320 190 27 27
BsmI GAATG_Cn'
Cuts at: 353 873 353
Size: 520 3712
BsmBI CGTCTCn'nnnn_
Cuts at: 1218 1218
Size: 4232
Bsp1286I G_dGCh'C
Cuts at: 323 412 439 663 712 899 926 977 1023
Size: 89 27 224 49 187 27 51 46
Cuts at: 1023 1246 1857 1950 2024 2214 2276 3006 323
Size: 223 611 93 74 190 62 730 1549
Fragments arranged by size:
1549 730 611 224 223 190 187 93
89 74 62 51 49 46 27 27
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1282 1496 1798 2179 1282
Size: 214 302 381 3335
Fragments arranged by size:
3335 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2524 2578 4062 4227 50
Size: 2474 54 1484 165 55
Fragments arranged by size:
2474 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2144 2144
Size: 4232
BsrFI r'CCGG_y
Cuts at: 2230 2411 2620 2230
Size: 181 209 3842
Fragments arranged by size:
3842 209 181
BssSI C'ACGA_G
Cuts at: 2503 2861 2503
Size: 358 3874
BstAPI GCAn_nnn'nTGC
Cuts at: 1165 1609 1165
Size: 444 3788
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstXI CCAn_nnnn'nTGG
Cuts at: 1176 1176
Size: 4232
BstYI r'GATC_y
Cuts at: 1 1257 1724 1747 2082 2328 3340 3426 3438
Size: 1256 467 23 335 246 1012 86 12
Cuts at: 3438 3784 1
Size: 346 449
Fragments arranged by size:
1256 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 305 305
Size: 4232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2182 2363 2526 2182
Size: 181 163 3888
Fragments arranged by size:
3888 181 163
BtsI GCAGTG_nn'
Cuts at: 1475 1475
Size: 4232
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 1938 2796 1938
Size: 858 3374
EaeI y'GGCC_r
Cuts at: 542 1178 1817 1991 2382 2409 3754 542
Size: 636 639 174 391 27 1345 1020
Fragments arranged by size:
1345 1020 639 636 391 174 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 206 240 270 288 1124 2255 2465 206
Size: 34 30 18 836 1131 210 1973
Fragments arranged by size:
1973 1131 836 210 34 30 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 219 2440 2750 2896 4042 219
Size: 2221 310 146 1146 409
Fragments arranged by size:
2221 1146 409 310 146
EcoICRI GAG'CTC
Cuts at: 410 437 924 1244 410
Size: 27 487 320 3398
Fragments arranged by size:
3398 487 320 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 2057 2489 3236 3504 979
Size: 1078 432 747 268 1707
Fragments arranged by size:
1707 1078 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 376 388 492 709 1371 376
Size: 12 104 217 662 3237
Fragments arranged by size:
3237 662 217 104 12
EcoRI G'AATT_C
Cuts at: 1000 1236 1000
Size: 236 3996
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 4232
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 1666 1914 2936 3915 1666
Size: 248 1022 979 1983
Fragments arranged by size:
1983 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 324 356 493 525 2074 2104 2106
Size: 32 156 32 137 32 1549 30 2
Cuts at: 2106 2136 136
Size: 30 2232
Fragments arranged by size:
2232 1549 156 137 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 144 1271 144
Size: 1127 3105
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MfeI C'AATT_G
Cuts at: 1148 1148
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3087 2903
Size: 184 4048
MscI TGG'CCA
Cuts at: 1180 1993 1180
Size: 813 3419
MslI CAynn'nnrTG
Cuts at: 722 1174 2221 2348 722
Size: 452 1047 127 2606
Fragments arranged by size:
2606 1047 452 127
MspA1I CmG'CkG
Cuts at: 1343 1657 2017 3030 3275 1343
Size: 314 360 1013 245 2300
Fragments arranged by size:
2300 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2413 2413
Size: 4232
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NgoMIV G'CCGG_C
Cuts at: 2411 2411
Size: 4232
NspI r_CATG'y
Cuts at: 780 1285 1785 2692 780
Size: 505 500 907 2320
Fragments arranged by size:
2320 907 505 500
PciI A'CATG_T
Cuts at: 2688 2688
Size: 4232
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PpuMI rG'GwC_Cy
Cuts at: 376 388 376
Size: 12 4220
PspOMI G'GGCC_C
Cuts at: 708 708
Size: 4232
PstI C_TGCA'G
Cuts at: 1279 1964 1279
Size: 685 3547
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 1657 2017 1657
Size: 360 3872
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 412 439 926 1246 412
Size: 27 487 320 3398
Fragments arranged by size:
3398 487 320 27
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 1049 3749 1049
Size: 2700 1532
SfcI C'TryA_G
Cuts at: 33 1275 1960 2953 3144 33
Size: 1242 685 993 191 1121
Fragments arranged by size:
1242 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 1254 1254
Size: 4232
SmlI C'TyrA_G
Cuts at: 652 1387 2302 2794 3056 652
Size: 735 915 492 262 1828
Fragments arranged by size:
1828 915 735 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 780 1285 1785 780
Size: 505 500 3227
Fragments arranged by size:
3227 505 500
StuI AGG'CCT
Cuts at: 746 746
Size: 4232
StyI C'CwwG_G
Cuts at: 916 1366 1720 916
Size: 450 354 3428
Fragments arranged by size:
3428 450 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 930 1815 1948 2407 2616 930
Size: 885 133 459 209 2546
Fragments arranged by size:
2546 885 459 209 133
TatI w'GTAC_w
Cuts at: 245 626 1047 3747 245
Size: 381 421 2700 730
Fragments arranged by size:
2700 730 421 381
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4200
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1727 1727
Size: 4232
XmaI C'CCGG_G
Cuts at: 1252 1252
Size: 4232
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI BaeI
BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsrGI
BssHII BstEII BstZ17I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI