Restriction map for
pF1KB7159
(Circular) (Six-base) MAPSORT of: pF1KB7159.seq Check: 2995 from: 1 to: 4208
pF1KB7159 4208 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 436 436
Size: 4208
AccI GT'mk_AC
Cuts at: 1246 1246
Size: 4208
Acc65I G'GTAC_C
Cuts at: 1224 1224
Size: 4208
AclI AA'CG_TT
Cuts at: 4048 4048
Size: 4208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 2465 3212 3480 2033
Size: 432 747 268 2761
Fragments arranged by size:
2761 747 432 268
AflII C'TTAA_G
Cuts at: 771 771
Size: 4208
AflIII A'CryG_T
Cuts at: 1560 2664 3641 1560
Size: 1104 977 2127
Fragments arranged by size:
2127 1104 977
AgeI A'CCGG_T
Cuts at: 2596 2596
Size: 4208
AleI CACnn'nnGTG
Cuts at: 446 446
Size: 4208
AlwNI CAG_nnn'CTG
Cuts at: 470 1513 3080 470
Size: 1043 1567 1598
Fragments arranged by size:
1598 1567 1043
ApaLI G'TGCA_C
Cuts at: 624 2978 624
Size: 2354 1854
ApoI r'AATT_y
Cuts at: 798 1113 1212 3561 3817 798
Size: 315 99 2349 256 1189
Fragments arranged by size:
2349 1189 315 256 99
AseI AT'TA_AT
Cuts at: 20 20
Size: 4208
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
AvaI C'yCGr_G
Cuts at: 1228 1228
Size: 4208
BamHI G'GATC_C
Cuts at: 899 1233 899
Size: 334 3874
BanI G'GyrC_C
Cuts at: 250 1224 1638 1886 1921 250
Size: 974 414 248 35 2537
Fragments arranged by size:
2537 974 414 248 35
BanII G_rGCy'C
Cuts at: 1222 2252 1222
Size: 1030 3178
BbeI G_GCGC'C
Cuts at: 1642 1890 1642
Size: 248 3960
BbsI GAAGACnn'nnnn_
Cuts at: 1160 1160
Size: 4208
BciVI GTATCCnnnnn_n'
Cuts at: 2106 2867 4208 2106
Size: 761 1341 2106
Fragments arranged by size:
2106 1341 761
BclI T'GATC_A
Cuts at: 236 1121 1728 236
Size: 885 607 2716
Fragments arranged by size:
2716 885 607
BglI GCCn_nnn'nGGC
Cuts at: 142 520 142
Size: 378 3830
BglII A'GATC_T
Cuts at: 947 1723 3760 947
Size: 776 2037 1395
Fragments arranged by size:
2037 1395 776
BlpI GC'TnA_GC
Cuts at: 1320 1320
Size: 4208
Bme1580I G_kGCm'C
Cuts at: 628 1833 1926 2982 628
Size: 1205 93 1056 1854
Fragments arranged by size:
1854 1205 1056 93
BmgBI CAC'GTC
Cuts at: 1097 1097
Size: 4208
BmrI ACTGGGnnnn_n'
Cuts at: 480 1420 1501 1836 3980 480
Size: 940 81 335 2144 708
Fragments arranged by size:
2144 940 708 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 4208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 606
Size: 4208
Bpu10I CC'TnA_GC
Cuts at: 340 604 340
Size: 264 3944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1384 2263 2755 3053 1384
Size: 879 492 298 2539
Fragments arranged by size:
2539 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3912 3912
Size: 4208
BsaAI yAC'GTr
Cuts at: 1561 1561
Size: 4208
BsaBI GATnn'nnATC
Cuts at: 6 923 1722 3759 6
Size: 917 799 2037 455
Fragments arranged by size:
2037 917 799 455
BsaHI Gr'CG_yC
Cuts at: 1639 1887 2589 4085 1639
Size: 248 702 1496 1762
Fragments arranged by size:
1762 1496 702 248
BsaWI w'CCGG_w
Cuts at: 716 1397 1918 2596 2870 3017 3428 716
Size: 681 521 678 274 147 411 1496
Fragments arranged by size:
1496 681 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 669 1633 2391 2968 3464 669
Size: 964 758 577 496 1413
Fragments arranged by size:
1413 964 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 408
Size: 4208
BsiEI CG_ry'CG
Cuts at: 104 1750 1796 3004 104
Size: 1646 46 1208 1308
Fragments arranged by size:
1646 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 329 628 1222 2000 2190 2982 329
Size: 299 594 778 190 792 1555
Fragments arranged by size:
1555 792 778 594 299 190
BsmI GAATG_Cn'
Cuts at: 1003 1003
Size: 4208
Bsp1286I G_dGCh'C
Cuts at: 329 628 1222 1833 1926 2000 2190 2252 2982
Size: 299 594 611 93 74 190 62 730
Cuts at: 2982 329
Size: 1555
Fragments arranged by size:
1555 730 611 594 299 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3428 3428
Size: 4208
BspHI T'CATG_A
Cuts at: 3384 3384
Size: 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 427 436 1258 1472 1774 2155 427
Size: 9 822 214 302 381 2480
Fragments arranged by size:
2480 822 381 302 214 9
BsrBI CCG'CTC
Cuts at: 50 2500 2554 4038 4203 50
Size: 2450 54 1484 165 55
Fragments arranged by size:
2450 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 452 2120 452
Size: 1668 2540
BsrFI r'CCGG_y
Cuts at: 2206 2387 2596 2206
Size: 181 209 3818
Fragments arranged by size:
3818 209 181
BssSI C'ACGA_G
Cuts at: 2479 2837 2479
Size: 358 3850
BstAPI GCAn_nnn'nTGC
Cuts at: 1585 1585
Size: 4208
BstBI TT'CG_AA
Cuts at: 2569 2569
Size: 4208
BstYI r'GATC_y
Cuts at: 1 899 947 1233 1700 1723 2058 2304 3316
Size: 898 48 286 467 23 335 246 1012
Cuts at: 3316 3402 3414 3760 1
Size: 86 12 346 449
Fragments arranged by size:
1012 898 467 449 346 335 286 246
86 48 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2158 2339 2502 2158
Size: 181 163 3864
Fragments arranged by size:
3864 181 163
BtsI GCAGTG_nn'
Cuts at: 329 1451 329
Size: 1122 3086
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 855 890 855
Size: 35 4173
DraI TTT'AAA
Cuts at: 1173 1207 1173
Size: 34 4174
DrdI GACnn_nn'nnGTC
Cuts at: 1914 2772 1914
Size: 858 3350
EaeI y'GGCC_r
Cuts at: 299 512 731 1082 1793 1967 2358 2385 3730
Size: 213 219 351 711 174 391 27 1345
Cuts at: 3730 299
Size: 777
Fragments arranged by size:
1345 777 711 391 351 219 213 174
27
EagI C'GGCC_G
Cuts at: 1793 1793
Size: 4208
EarI CTCTTCn'nnn_
Cuts at: 499 2231 2441 499
Size: 1732 210 2266
Fragments arranged by size:
2266 1732 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2416 2726 2872 4018 2416
Size: 310 146 1146 2606
Fragments arranged by size:
2606 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1220 1220
Size: 4208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 2033 2465 3212 3480 606
Size: 1427 432 747 268 1334
Fragments arranged by size:
1427 1334 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 391 894 391
Size: 503 3705
EcoO109I rG'GnC_Cy
Cuts at: 1347 1347
Size: 4208
EcoRI G'AATT_C
Cuts at: 798 1113 1212 798
Size: 315 99 3794
Fragments arranged by size:
3794 315 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 4208
FspI TGC'GCA
Cuts at: 1989 1989
Size: 4208
HaeII r_GCGC'y
Cuts at: 1642 1890 2912 3891 1642
Size: 248 1022 979 1959
Fragments arranged by size:
1959 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1035 1067 1113 1145 2050 2080 2082 2112 1035
Size: 32 46 32 905 30 2 30 3131
Fragments arranged by size:
3131 905 46 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1247 1247
Size: 4208
HindIII A'AGCT_T
Cuts at: 373 373
Size: 4208
KasI G'GCGC_C
Cuts at: 1638 1886 1638
Size: 248 3960
KpnI G_GTAC'C
Cuts at: 1228 1228
Size: 4208
MluI A'CGCG_T
Cuts at: 3641 3641
Size: 4208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2879 3063 2879
Size: 184 4024
MscI TGG'CCA
Cuts at: 1969 1969
Size: 4208
MslI CAynn'nnrTG
Cuts at: 184 358 446 568 641 2197 2324 184
Size: 174 88 122 73 1556 127 2068
Fragments arranged by size:
2068 1556 174 127 122 88 73
MspA1I CmG'CkG
Cuts at: 1319 1633 1993 3006 3251 1319
Size: 314 360 1013 245 2276
Fragments arranged by size:
2276 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2389 2389
Size: 4208
NarI GG'CG_CC
Cuts at: 1639 1887 1639
Size: 248 3960
NgoMIV G'CCGG_C
Cuts at: 2387 2387
Size: 4208
NspI r_CATG'y
Cuts at: 1261 1761 2668 1261
Size: 500 907 2801
Fragments arranged by size:
2801 907 500
PciI A'CATG_T
Cuts at: 2664 2664
Size: 4208
PmeI GTTT'AAAC
Cuts at: 1207 1207
Size: 4208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 4176
PstI C_TGCA'G
Cuts at: 1255 1940 1255
Size: 685 3523
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4208
PvuII CAG'CTG
Cuts at: 1633 1993 1633
Size: 360 3848
RsrII CG'GwC_CG
Cuts at: 2403 2403
Size: 4208
SacI G_AGCT'C
Cuts at: 1222 1222
Size: 4208
SalI G'TCGA_C
Cuts at: 1245 1245
Size: 4208
SapI GCTCTTCn'nnn_
Cuts at: 2231 2441 2231
Size: 210 3998
SbfI CC_TGCA'GG
Cuts at: 1255 1255
Size: 4208
ScaI AGT'ACT
Cuts at: 3725 3725
Size: 4208
SfcI C'TryA_G
Cuts at: 33 1251 1936 2929 3120 33
Size: 1218 685 993 191 1121
Fragments arranged by size:
1218 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 520 520
Size: 4208
SfoI GGC'GCC
Cuts at: 1640 1888 1640
Size: 248 3960
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
SmaI CCC'GGG
Cuts at: 1230 1230
Size: 4208
SmlI C'TyrA_G
Cuts at: 771 1363 2278 2770 3032 771
Size: 592 915 492 262 1947
Fragments arranged by size:
1947 915 592 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4208
SphI G_CATG'C
Cuts at: 1261 1761 1261
Size: 500 3708
StyI C'CwwG_G
Cuts at: 1342 1696 1342
Size: 354 3854
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 661 944 988 1791 1924 2383 2592 661
Size: 283 44 803 133 459 209 2277
Fragments arranged by size:
2277 803 459 283 209 133 44
TatI w'GTAC_w
Cuts at: 3723 3723
Size: 4208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3725 3757 3725
Size: 32 4176
Tth111I GACn'n_nGTC
Cuts at: 2005 2005
Size: 4208
XbaI T'CTAG_A
Cuts at: 921 1239 921
Size: 318 3890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1703 1703
Size: 4208
XmaI C'CCGG_G
Cuts at: 1228 1228
Size: 4208
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BsaXI BseRI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI DraIII EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI