Restriction map for
pF1KB7158
(Circular) (Six-base) MAPSORT of: pF1KB7158.seq Check: 4792 from: 1 to: 4202
pF1KB7158 4202 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 523 523
Size: 4202
AatII G_ACGT'C
Cuts at: 659 659
Size: 4202
AccI GT'mk_AC
Cuts at: 199 1240 199
Size: 1041 3161
Acc65I G'GTAC_C
Cuts at: 533 1218 533
Size: 685 3517
AclI AA'CG_TT
Cuts at: 4042 4042
Size: 4202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 892 2027 2459 3206 3474 844
Size: 48 1135 432 747 268 1572
Fragments arranged by size:
1572 1135 747 432 268 48
AflIII A'CryG_T
Cuts at: 488 1554 2658 3635 488
Size: 1066 1104 977 1055
Fragments arranged by size:
1104 1066 1055 977
AgeI A'CCGG_T
Cuts at: 1076 2590 1076
Size: 1514 2688
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 257 257
Size: 4202
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1149 1181 1149
Size: 32 4170
AlwNI CAG_nnn'CTG
Cuts at: 886 1507 3074 886
Size: 621 1567 2014
Fragments arranged by size:
2014 1567 621
ApaLI G'TGCA_C
Cuts at: 2972 2972
Size: 4202
ApoI r'AATT_y
Cuts at: 1206 3555 3811 1206
Size: 2349 256 1597
Fragments arranged by size:
2349 1597 256
AseI AT'TA_AT
Cuts at: 20 968 20
Size: 948 3254
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
AvaI C'yCGr_G
Cuts at: 1222 1222
Size: 4202
BamHI G'GATC_C
Cuts at: 1227 1227
Size: 4202
BanI G'GyrC_C
Cuts at: 533 1218 1632 1880 1915 533
Size: 685 414 248 35 2820
Fragments arranged by size:
2820 685 414 248 35
BanII G_rGCy'C
Cuts at: 1216 2246 1216
Size: 1030 3172
BbeI G_GCGC'C
Cuts at: 1636 1884 1636
Size: 248 3954
BbsI GAAGACnn'nnnn_
Cuts at: 151 832 151
Size: 681 3521
BciVI GTATCCnnnnn_n'
Cuts at: 2100 2861 4202 2100
Size: 761 1341 2100
Fragments arranged by size:
2100 1341 761
BclI T'GATC_A
Cuts at: 1722 1722
Size: 4202
BglII A'GATC_T
Cuts at: 1717 3754 1717
Size: 2037 2165
BlpI GC'TnA_GC
Cuts at: 1314 1314
Size: 4202
Bme1580I G_kGCm'C
Cuts at: 1827 1920 2976 1827
Size: 93 1056 3053
Fragments arranged by size:
3053 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1414 1495 1830 3974 1414
Size: 81 335 2144 1642
Fragments arranged by size:
2144 1642 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 4202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1378 2257 2749 3047 1378
Size: 879 492 298 2533
Fragments arranged by size:
2533 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1177 3906 1177
Size: 2729 1473
BsaAI yAC'GTr
Cuts at: 1555 1555
Size: 4202
BsaBI GATnn'nnATC
Cuts at: 6 1716 3753 6
Size: 1710 2037 455
Fragments arranged by size:
2037 1710 455
BsaHI Gr'CG_yC
Cuts at: 656 1633 1881 2583 4079 656
Size: 977 248 702 1496 779
Fragments arranged by size:
1496 977 779 702 248
BsaWI w'CCGG_w
Cuts at: 1076 1391 1912 2590 2864 3011 3422 1076
Size: 315 521 678 274 147 411 1856
Fragments arranged by size:
1856 678 521 411 315 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1076 1106 1076
Size: 30 4172
BseYI C'CCAG_C
Cuts at: 733 1627 2385 2962 3458 733
Size: 894 758 577 496 1477
Fragments arranged by size:
1477 894 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1744 1790 2998 104
Size: 1640 46 1208 1308
Fragments arranged by size:
1640 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1216 1994 2184 2976 1216
Size: 778 190 792 2442
Fragments arranged by size:
2442 792 778 190
BsmI GAATG_Cn'
Cuts at: 690 690
Size: 4202
BsmBI CGTCTCn'nnnn_
Cuts at: 647 647
Size: 4202
Bsp1286I G_dGCh'C
Cuts at: 1216 1827 1920 1994 2184 2246 2976 1216
Size: 611 93 74 190 62 730 2442
Fragments arranged by size:
2442 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3422 3422
Size: 4202
BspHI T'CATG_A
Cuts at: 3378 3378
Size: 4202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 523 1252 1466 1768 2149 523
Size: 729 214 302 381 2576
Fragments arranged by size:
2576 729 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2494 2548 4032 4197 50
Size: 2444 54 1484 165 55
Fragments arranged by size:
2444 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 631 2114 631
Size: 1483 2719
BsrFI r'CCGG_y
Cuts at: 1076 2200 2381 2590 1076
Size: 1124 181 209 2688
Fragments arranged by size:
2688 1124 209 181
BssSI C'ACGA_G
Cuts at: 2473 2831 2473
Size: 358 3844
BstAPI GCAn_nnn'nTGC
Cuts at: 1579 1579
Size: 4202
BstBI TT'CG_AA
Cuts at: 2563 2563
Size: 4202
BstXI CCAn_nnnn'nTGG
Cuts at: 1023 1121 1023
Size: 98 4104
BstYI r'GATC_y
Cuts at: 1 1227 1694 1717 2052 2298 3310 3396 3408
Size: 1226 467 23 335 246 1012 86 12
Cuts at: 3408 3754 1
Size: 346 449
Fragments arranged by size:
1226 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2152 2333 2496 2152
Size: 181 163 3858
Fragments arranged by size:
3858 181 163
BtsI GCAGTG_nn'
Cuts at: 1104 1445 1104
Size: 341 3861
DraI TTT'AAA
Cuts at: 1201 1201
Size: 4202
DrdI GACnn_nn'nnGTC
Cuts at: 1191 1908 2766 1191
Size: 717 858 2627
Fragments arranged by size:
2627 858 717
EaeI y'GGCC_r
Cuts at: 1787 1961 2352 2379 3724 1787
Size: 174 391 27 1345 2265
Fragments arranged by size:
2265 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1787 1787
Size: 4202
EarI CTCTTCn'nnn_
Cuts at: 401 2225 2435 401
Size: 1824 210 2168
Fragments arranged by size:
2168 1824 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2410 2720 2866 4012 2410
Size: 310 146 1146 2600
Fragments arranged by size:
2600 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1214 1214
Size: 4202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 892 2027 2459 3206 3474 844
Size: 48 1135 432 747 268 1572
Fragments arranged by size:
1572 1135 747 432 268 48
EcoO109I rG'GnC_Cy
Cuts at: 328 1341 328
Size: 1013 3189
EcoRI G'AATT_C
Cuts at: 1206 1206
Size: 4202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 4202
FspI TGC'GCA
Cuts at: 1983 1983
Size: 4202
HaeII r_GCGC'y
Cuts at: 1636 1884 2906 3885 1636
Size: 248 1022 979 1953
Fragments arranged by size:
1953 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 272 304 1132 1164 2044 2074 2076 2106 272
Size: 32 828 32 880 30 2 30 2368
Fragments arranged by size:
2368 880 828 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1241 1241
Size: 4202
KasI G'GCGC_C
Cuts at: 1632 1880 1632
Size: 248 3954
KpnI G_GTAC'C
Cuts at: 537 1222 537
Size: 685 3517
MluI A'CGCG_T
Cuts at: 3635 3635
Size: 4202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1050 1107 2873 3057 1050
Size: 57 1766 184 2195
Fragments arranged by size:
2195 1766 184 57
MscI TGG'CCA
Cuts at: 1963 1963
Size: 4202
MslI CAynn'nnrTG
Cuts at: 239 517 2191 2318 239
Size: 278 1674 127 2123
Fragments arranged by size:
2123 1674 278 127
MspA1I CmG'CkG
Cuts at: 155 733 883 1040 1313 1627 1987 3000 3245
Size: 578 150 157 273 314 360 1013 245
Cuts at: 3245 155
Size: 1112
Fragments arranged by size:
1112 1013 578 360 314 273 245 157
150
NaeI GCC'GGC
Cuts at: 2383 2383
Size: 4202
NarI GG'CG_CC
Cuts at: 1633 1881 1633
Size: 248 3954
NgoMIV G'CCGG_C
Cuts at: 2381 2381
Size: 4202
NspI r_CATG'y
Cuts at: 492 797 1255 1755 2662 492
Size: 305 458 500 907 2032
Fragments arranged by size:
2032 907 500 458 305
PciI A'CATG_T
Cuts at: 488 2658 488
Size: 2170 2032
PfoI T'CCnGG_A
Cuts at: 938 938
Size: 4202
PmeI GTTT'AAAC
Cuts at: 1201 1201
Size: 4202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 4170
PpuMI rG'GwC_Cy
Cuts at: 328 328
Size: 4202
PstI C_TGCA'G
Cuts at: 1249 1934 1249
Size: 685 3517
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4202
PvuII CAG'CTG
Cuts at: 733 883 1040 1627 1987 733
Size: 150 157 587 360 2948
Fragments arranged by size:
2948 587 360 157 150
RsrII CG'GwC_CG
Cuts at: 2397 2397
Size: 4202
SacI G_AGCT'C
Cuts at: 1216 1216
Size: 4202
SalI G'TCGA_C
Cuts at: 1239 1239
Size: 4202
SapI GCTCTTCn'nnn_
Cuts at: 2225 2435 2225
Size: 210 3992
SbfI CC_TGCA'GG
Cuts at: 1249 1249
Size: 4202
ScaI AGT'ACT
Cuts at: 3719 3719
Size: 4202
SfcI C'TryA_G
Cuts at: 33 1245 1930 2923 3114 33
Size: 1212 685 993 191 1121
Fragments arranged by size:
1212 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1634 1882 1634
Size: 248 3954
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
SmaI CCC'GGG
Cuts at: 1224 1224
Size: 4202
SmlI C'TyrA_G
Cuts at: 1357 2272 2764 3026 1357
Size: 915 492 262 2533
Fragments arranged by size:
2533 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4202
SphI G_CATG'C
Cuts at: 1255 1755 1255
Size: 500 3702
SspI AAT'ATT
Cuts at: 294 782 294
Size: 488 3714
StyI C'CwwG_G
Cuts at: 759 1128 1336 1690 759
Size: 369 208 354 3271
Fragments arranged by size:
3271 369 354 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 949 1785 1918 2377 2586 949
Size: 836 133 459 209 2565
Fragments arranged by size:
2565 836 459 209 133
TatI w'GTAC_w
Cuts at: 3717 3717
Size: 4202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 843 875 3719 3751 843
Size: 32 2844 32 1294
Fragments arranged by size:
2844 1294 32 32
Tth111I GACn'n_nGTC
Cuts at: 865 1999 865
Size: 1134 3068
XbaI T'CTAG_A
Cuts at: 1233 1233
Size: 4202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 730 1697 730
Size: 967 3235
XmaI C'CCGG_G
Cuts at: 1222 1222
Size: 4202
ZraI GAC'GTC
Cuts at: 657 657
Size: 4202
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I
BseRI BsgI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI