Restriction map for
pF1KB7157
(Circular) (Six-base) MAPSORT of: pF1KB7157.seq Check: 3500 from: 1 to: 4193
pF1KB7157 4193 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 786 786
Size: 4193
AccI GT'mk_AC
Cuts at: 1231 1231
Size: 4193
Acc65I G'GTAC_C
Cuts at: 1209 1209
Size: 4193
AclI AA'CG_TT
Cuts at: 4033 4033
Size: 4193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 2018 2450 3197 3465 451
Size: 1567 432 747 268 1179
Fragments arranged by size:
1567 1179 747 432 268
AflIII A'CryG_T
Cuts at: 1545 2649 3626 1545
Size: 1104 977 2112
Fragments arranged by size:
2112 1104 977
AgeI A'CCGG_T
Cuts at: 2581 2581
Size: 4193
AhdI GACnn_n'nnGTC
Cuts at: 629 629
Size: 4193
AlwNI CAG_nnn'CTG
Cuts at: 466 641 1132 1498 3065 466
Size: 175 491 366 1567 1594
Fragments arranged by size:
1594 1567 491 366 175
ApaLI G'TGCA_C
Cuts at: 225 2963 225
Size: 2738 1455
ApoI r'AATT_y
Cuts at: 384 1197 3546 3802 384
Size: 813 2349 256 775
Fragments arranged by size:
2349 813 775 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 1104 1213 1104
Size: 109 4084
AvrII C'CTAG_G
Cuts at: 846 846
Size: 4193
BamHI G'GATC_C
Cuts at: 1218 1218
Size: 4193
BanI G'GyrC_C
Cuts at: 798 1209 1623 1871 1906 798
Size: 411 414 248 35 3085
Fragments arranged by size:
3085 414 411 248 35
BanII G_rGCy'C
Cuts at: 1207 2237 1207
Size: 1030 3163
BbeI G_GCGC'C
Cuts at: 1627 1875 1627
Size: 248 3945
BbsI GAAGACnn'nnnn_
Cuts at: 211 414 211
Size: 203 3990
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 488 522 488
Size: 34 4159
BciVI GTATCCnnnnn_n'
Cuts at: 2091 2852 4193 2091
Size: 761 1341 2091
Fragments arranged by size:
2091 1341 761
BclI T'GATC_A
Cuts at: 1713 1713
Size: 4193
BglI GCCn_nnn'nGGC
Cuts at: 901 901
Size: 4193
BglII A'GATC_T
Cuts at: 1167 1708 3745 1167
Size: 541 2037 1615
Fragments arranged by size:
2037 1615 541
BlpI GC'TnA_GC
Cuts at: 682 1305 682
Size: 623 3570
Bme1580I G_kGCm'C
Cuts at: 229 252 558 1818 1911 2967 229
Size: 23 306 1260 93 1056 1455
Fragments arranged by size:
1455 1260 1056 306 93 23
BmrI ACTGGGnnnn_n'
Cuts at: 880 1405 1486 1821 3965 880
Size: 525 81 335 2144 1108
Fragments arranged by size:
2144 1108 525 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1044 2560 1044
Size: 1516 2677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 274
Size: 4193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1369 2248 2740 3038 1369
Size: 879 492 298 2524
Fragments arranged by size:
2524 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3897 3897
Size: 4193
BsaAI yAC'GTr
Cuts at: 1546 1546
Size: 4193
BsaBI GATnn'nnATC
Cuts at: 6 1707 3744 6
Size: 1701 2037 455
Fragments arranged by size:
2037 1701 455
BsaHI Gr'CG_yC
Cuts at: 1624 1872 2574 4070 1624
Size: 248 702 1496 1747
Fragments arranged by size:
1747 1496 702 248
BsaWI w'CCGG_w
Cuts at: 715 1382 1903 2581 2855 3002 3413 715
Size: 667 521 678 274 147 411 1495
Fragments arranged by size:
1495 678 667 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 859 1020 859
Size: 161 4032
BseYI C'CCAG_C
Cuts at: 119 337 1618 2376 2953 3449 119
Size: 218 1281 758 577 496 863
Fragments arranged by size:
1281 863 758 577 496 218
BsiEI CG_ry'CG
Cuts at: 104 1735 1781 2989 104
Size: 1631 46 1208 1308
Fragments arranged by size:
1631 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 229 272 869 1207 1985 2175 2967 229
Size: 43 597 338 778 190 792 1455
Fragments arranged by size:
1455 792 778 597 338 190 43
BsmI GAATG_Cn'
Cuts at: 121 121
Size: 4193
Bsp1286I G_dGCh'C
Cuts at: 229 252 272 558 869 1207 1818 1911 1985
Size: 23 20 286 311 338 611 93 74
Cuts at: 1985 2175 2237 2967 229
Size: 190 62 730 1455
Fragments arranged by size:
1455 730 611 338 311 286 190 93
74 62 23 20
BspEI T'CCGG_A
Cuts at: 715 3413 715
Size: 2698 1495
BspHI T'CATG_A
Cuts at: 3369 3369
Size: 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 614 686 786 1243 1457 1759 2140 614
Size: 72 100 457 214 302 381 2667
Fragments arranged by size:
2667 457 381 302 214 100 72
BsrBI CCG'CTC
Cuts at: 50 1028 2485 2539 4023 4188 50
Size: 978 1457 54 1484 165 55
Fragments arranged by size:
1484 1457 978 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2105 2105
Size: 4193
BsrFI r'CCGG_y
Cuts at: 2191 2372 2581 2191
Size: 181 209 3803
Fragments arranged by size:
3803 209 181
BssSI C'ACGA_G
Cuts at: 2464 2822 2464
Size: 358 3835
BstAPI GCAn_nnn'nTGC
Cuts at: 1570 1570
Size: 4193
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstEII G'GTnAC_C
Cuts at: 561 561
Size: 4193
BstYI r'GATC_y
Cuts at: 1 1022 1108 1167 1218 1685 1708 2043 2289
Size: 1021 86 59 51 467 23 335 246
Cuts at: 2289 3301 3387 3399 3745 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1021 1012 467 449 346 335 246 86
86 59 51 23 12
BtgI C'CryG_G
Cuts at: 107 399 546 983 1150 107
Size: 292 147 437 167 3150
Fragments arranged by size:
3150 437 292 167 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 684 2143 2324 2487 684
Size: 1459 181 163 2390
Fragments arranged by size:
2390 1459 181 163
BtsI GCAGTG_nn'
Cuts at: 1041 1436 1041
Size: 395 3798
DraI TTT'AAA
Cuts at: 1192 1192
Size: 4193
DraIII CAC_nnn'GTG
Cuts at: 1151 1151
Size: 4193
DrdI GACnn_nn'nnGTC
Cuts at: 1899 2757 1899
Size: 858 3335
EaeI y'GGCC_r
Cuts at: 353 767 780 836 914 1153 1778 1952 2343
Size: 414 13 56 78 239 625 174 391
Cuts at: 2343 2370 3715 353
Size: 27 1345 831
Fragments arranged by size:
1345 831 625 414 391 239 174 78
56 27 13
EagI C'GGCC_G
Cuts at: 1778 1778
Size: 4193
EarI CTCTTCn'nnn_
Cuts at: 236 2216 2426 236
Size: 1980 210 2003
Fragments arranged by size:
2003 1980 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 289 2401 2711 2857 4003 289
Size: 2112 310 146 1146 479
Fragments arranged by size:
2112 1146 479 310 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 451 2018 2450 3197 3465 274
Size: 177 1567 432 747 268 1002
Fragments arranged by size:
1567 1002 747 432 268 177
EcoO109I rG'GnC_Cy
Cuts at: 582 638 1074 1332 582
Size: 56 436 258 3443
Fragments arranged by size:
3443 436 258 56
EcoRI G'AATT_C
Cuts at: 1197 1197
Size: 4193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 171 438 963 2212 171
Size: 267 525 1249 2152
Fragments arranged by size:
2152 1249 525 267
FspI TGC'GCA
Cuts at: 1974 1974
Size: 4193
HaeII r_GCGC'y
Cuts at: 1627 1875 2897 3876 1627
Size: 248 1022 979 1944
Fragments arranged by size:
1944 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 615 647 1100 1132 2035 2065 2067 2097 615
Size: 32 453 32 903 30 2 30 2711
Fragments arranged by size:
2711 903 453 32 32 30 30 2
HincII GTy'rAC
Cuts at: 990 1232 990
Size: 242 3951
KasI G'GCGC_C
Cuts at: 1623 1871 1623
Size: 248 3945
KpnI G_GTAC'C
Cuts at: 1213 1213
Size: 4193
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 188 2864 3048 188
Size: 2676 184 1333
Fragments arranged by size:
2676 1333 184
MscI TGG'CCA
Cuts at: 355 769 782 838 916 1155 1954 355
Size: 414 13 56 78 239 799 2594
Fragments arranged by size:
2594 799 414 239 78 56 13
MslI CAynn'nnrTG
Cuts at: 761 2182 2309 761
Size: 1421 127 2645
Fragments arranged by size:
2645 1421 127
MspA1I CmG'CkG
Cuts at: 211 1304 1618 1978 2991 3236 211
Size: 1093 314 360 1013 245 1168
Fragments arranged by size:
1168 1093 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 4193
NarI GG'CG_CC
Cuts at: 1624 1872 1624
Size: 248 3945
NcoI C'CATG_G
Cuts at: 107 399 983 107
Size: 292 584 3317
Fragments arranged by size:
3317 584 292
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 4193
NspI r_CATG'y
Cuts at: 374 742 766 1246 1746 2653 374
Size: 368 24 480 500 907 1914
Fragments arranged by size:
1914 907 500 480 368 24
PasI CC'CwG_GG
Cuts at: 567 567
Size: 4193
PciI A'CATG_T
Cuts at: 2649 2649
Size: 4193
PflMI CCAn_nnn'nTGG
Cuts at: 405 912 405
Size: 507 3686
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 4161
PpuMI rG'GwC_Cy
Cuts at: 582 582
Size: 4193
PstI C_TGCA'G
Cuts at: 1240 1925 1240
Size: 685 3508
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 211 1618 1978 211
Size: 1407 360 2426
Fragments arranged by size:
2426 1407 360
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SalI G'TCGA_C
Cuts at: 1230 1230
Size: 4193
SapI GCTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 3710 3710
Size: 4193
SexAI A'CCwGG_T
Cuts at: 932 932
Size: 4193
SfcI C'TryA_G
Cuts at: 33 1236 1921 2914 3105 33
Size: 1203 685 993 191 1121
Fragments arranged by size:
1203 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 901 901
Size: 4193
SfoI GGC'GCC
Cuts at: 1625 1873 1625
Size: 248 3945
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 1215 1215
Size: 4193
SmlI C'TyrA_G
Cuts at: 1348 2263 2755 3017 1348
Size: 915 492 262 2524
Fragments arranged by size:
2524 915 492 262
SpeI A'CTAG_T
Cuts at: 67 594 67
Size: 527 3666
SphI G_CATG'C
Cuts at: 374 1246 1746 374
Size: 872 500 2821
Fragments arranged by size:
2821 872 500
StuI AGG'CCT
Cuts at: 946 946
Size: 4193
StyI C'CwwG_G
Cuts at: 107 146 399 413 846 983 1077 1327 1681
Size: 39 253 14 433 137 94 250 354
Cuts at: 1681 107
Size: 2619
Fragments arranged by size:
2619 433 354 253 250 137 94 39
14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 111 512 1776 1909 2368 2577 111
Size: 401 1264 133 459 209 1727
Fragments arranged by size:
1727 1264 459 401 209 133
TatI w'GTAC_w
Cuts at: 3708 3708
Size: 4193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1050 1082 3710 3742 1050
Size: 32 2628 32 1501
Fragments arranged by size:
2628 1501 32 32
Tth111I GACn'n_nGTC
Cuts at: 934 1990 934
Size: 1056 3137
XbaI T'CTAG_A
Cuts at: 1170 1224 1170
Size: 54 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 153 397 1688 153
Size: 244 1291 2658
Fragments arranged by size:
2658 1291 244
XmaI C'CCGG_G
Cuts at: 1213 1213
Size: 4193
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI