Restriction map for
pF1KB7154
(Circular) (Six-base) MAPSORT of: pF1KB7154.seq Check: 6713 from: 1 to: 4172
pF1KB7154 4172 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 446 1210 446
Size: 764 3408
Acc65I G'GTAC_C
Cuts at: 1188 1188
Size: 4172
AclI AA'CG_TT
Cuts at: 4012 4012
Size: 4172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 720 1997 2429 3176 3444 720
Size: 1277 432 747 268 1448
Fragments arranged by size:
1448 1277 747 432 268
AflIII A'CryG_T
Cuts at: 683 828 1524 2628 3605 683
Size: 145 696 1104 977 1250
Fragments arranged by size:
1250 1104 977 696 145
AgeI A'CCGG_T
Cuts at: 2560 2560
Size: 4172
AlwNI CAG_nnn'CTG
Cuts at: 1477 3044 1477
Size: 1567 2605
ApaI G_GGCC'C
Cuts at: 507 1080 507
Size: 573 3599
ApaLI G'TGCA_C
Cuts at: 2942 2942
Size: 4172
ApoI r'AATT_y
Cuts at: 585 788 1176 3525 3781 585
Size: 203 388 2349 256 976
Fragments arranged by size:
2349 976 388 256 203
AseI AT'TA_AT
Cuts at: 20 20
Size: 4172
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4172
AvaI C'yCGr_G
Cuts at: 1192 1192
Size: 4172
BamHI G'GATC_C
Cuts at: 1197 1197
Size: 4172
BanI G'GyrC_C
Cuts at: 1188 1602 1850 1885 1188
Size: 414 248 35 3475
Fragments arranged by size:
3475 414 248 35
BanII G_rGCy'C
Cuts at: 507 1080 1186 2216 507
Size: 573 106 1030 2463
Fragments arranged by size:
2463 1030 573 106
BbeI G_GCGC'C
Cuts at: 1606 1854 1606
Size: 248 3924
BbsI GAAGACnn'nnnn_
Cuts at: 213 825 213
Size: 612 3560
BciVI GTATCCnnnnn_n'
Cuts at: 2070 2831 4172 2070
Size: 761 1341 2070
Fragments arranged by size:
2070 1341 761
BclI T'GATC_A
Cuts at: 452 1692 452
Size: 1240 2932
BglII A'GATC_T
Cuts at: 1687 3724 1687
Size: 2037 2135
BlpI GC'TnA_GC
Cuts at: 1284 1284
Size: 4172
Bme1580I G_kGCm'C
Cuts at: 507 1080 1797 1890 2946 507
Size: 573 717 93 1056 1733
Fragments arranged by size:
1733 1056 717 573 93
BmrI ACTGGGnnnn_n'
Cuts at: 1384 1465 1800 3944 1384
Size: 81 335 2144 1612
Fragments arranged by size:
2144 1612 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2539 2539
Size: 4172
Bpu10I CC'TnA_GC
Cuts at: 158 158
Size: 4172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1348 2227 2719 3017 1348
Size: 879 492 298 2503
Fragments arranged by size:
2503 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 360 3876 360
Size: 3516 656
BsaAI yAC'GTr
Cuts at: 829 1525 829
Size: 696 3476
BsaBI GATnn'nnATC
Cuts at: 6 424 457 1686 3723 6
Size: 418 33 1229 2037 455
Fragments arranged by size:
2037 1229 455 418 33
BsaHI Gr'CG_yC
Cuts at: 1603 1851 2553 4049 1603
Size: 248 702 1496 1726
Fragments arranged by size:
1726 1496 702 248
BsaWI w'CCGG_w
Cuts at: 266 1361 1882 2560 2834 2981 3392 266
Size: 1095 521 678 274 147 411 1046
Fragments arranged by size:
1095 1046 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1138 1168 1138
Size: 30 4142
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 1159 160
Size: 999 3173
BseYI C'CCAG_C
Cuts at: 1597 2355 2932 3428 1597
Size: 758 577 496 2341
Fragments arranged by size:
2341 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1714 1760 2968 104
Size: 1610 46 1208 1308
Fragments arranged by size:
1610 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 155 1186 1964 2154 2946 155
Size: 1031 778 190 792 1381
Fragments arranged by size:
1381 1031 792 778 190
BsmI GAATG_Cn'
Cuts at: 649 890 649
Size: 241 3931
BsmBI CGTCTCn'nnnn_
Cuts at: 1141 1141
Size: 4172
Bsp1286I G_dGCh'C
Cuts at: 155 507 1080 1186 1797 1890 1964 2154 2216
Size: 352 573 106 611 93 74 190 62
Cuts at: 2216 2946 155
Size: 730 1381
Fragments arranged by size:
1381 730 611 573 352 190 106 93
74 62
BspEI T'CCGG_A
Cuts at: 266 3392 266
Size: 3126 1046
BspHI T'CATG_A
Cuts at: 3348 3348
Size: 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1222 1436 1738 2119 1222
Size: 214 302 381 3275
Fragments arranged by size:
3275 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2464 2518 4002 4167 50
Size: 2414 54 1484 165 55
Fragments arranged by size:
2414 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 193 243 461 1169 2084 193
Size: 50 218 708 915 2281
Fragments arranged by size:
2281 915 708 218 50
BsrFI r'CCGG_y
Cuts at: 2170 2351 2560 2170
Size: 181 209 3782
Fragments arranged by size:
3782 209 181
BssSI C'ACGA_G
Cuts at: 2443 2801 2443
Size: 358 3814
BstAPI GCAn_nnn'nTGC
Cuts at: 1549 1549
Size: 4172
BstBI TT'CG_AA
Cuts at: 2533 2533
Size: 4172
BstXI CCAn_nnnn'nTGG
Cuts at: 528 1068 528
Size: 540 3632
BstYI r'GATC_y
Cuts at: 1 185 1197 1664 1687 2022 2268 3280 3366
Size: 184 1012 467 23 335 246 1012 86
Cuts at: 3366 3378 3724 1
Size: 12 346 449
Fragments arranged by size:
1012 1012 467 449 346 335 246 184
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1142 1142
Size: 4172
BtgI C'CryG_G
Cuts at: 107 204 695 107
Size: 97 491 3584
Fragments arranged by size:
3584 491 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 499 762 2122 2303 2466 499
Size: 263 1360 181 163 2205
Fragments arranged by size:
2205 1360 263 181 163
BtsI GCAGTG_nn'
Cuts at: 662 1415 662
Size: 753 3419
DraI TTT'AAA
Cuts at: 1171 1171
Size: 4172
DrdI GACnn_nn'nnGTC
Cuts at: 1878 2736 1878
Size: 858 3314
EaeI y'GGCC_r
Cuts at: 168 530 698 1757 1931 2322 2349 3694 168
Size: 362 168 1059 174 391 27 1345 646
Fragments arranged by size:
1345 1059 646 391 362 174 168 27
EagI C'GGCC_G
Cuts at: 1757 1757
Size: 4172
EarI CTCTTCn'nnn_
Cuts at: 2195 2405 2195
Size: 210 3962
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2380 2690 2836 3982 2380
Size: 310 146 1146 2570
Fragments arranged by size:
2570 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1184 1184
Size: 4172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 720 1997 2429 3176 3444 720
Size: 1277 432 747 268 1448
Fragments arranged by size:
1448 1277 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1084 1084
Size: 4172
EcoO109I rG'GnC_Cy
Cuts at: 228 1076 1077 1089 1311 228
Size: 848 1 12 222 3089
Fragments arranged by size:
3089 848 222 12 1
EcoRI G'AATT_C
Cuts at: 1176 1176
Size: 4172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 4172
FspI TGC'GCA
Cuts at: 1953 1953
Size: 4172
HaeII r_GCGC'y
Cuts at: 1606 1854 2876 3855 1606
Size: 248 1022 979 1923
Fragments arranged by size:
1923 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2014 2044 2046 2076 2014
Size: 30 2 30 4110
Fragments arranged by size:
4110 30 30 2
HincII GTy'rAC
Cuts at: 285 1211 285
Size: 926 3246
KasI G'GCGC_C
Cuts at: 1602 1850 1602
Size: 248 3924
KpnI G_GTAC'C
Cuts at: 1192 1192
Size: 4172
MluI A'CGCG_T
Cuts at: 3605 3605
Size: 4172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2843 3027 2843
Size: 184 3988
MscI TGG'CCA
Cuts at: 170 532 700 1933 170
Size: 362 168 1233 2409
Fragments arranged by size:
2409 1233 362 168
MslI CAynn'nnrTG
Cuts at: 396 694 895 2161 2288 396
Size: 298 201 1266 127 2280
Fragments arranged by size:
2280 1266 298 201 127
MspA1I CmG'CkG
Cuts at: 476 992 1283 1597 1957 2970 3215 476
Size: 516 291 314 360 1013 245 1433
Fragments arranged by size:
1433 1013 516 360 314 291 245
NaeI GCC'GGC
Cuts at: 2353 2353
Size: 4172
NarI GG'CG_CC
Cuts at: 1603 1851 1603
Size: 248 3924
NcoI C'CATG_G
Cuts at: 107 695 107
Size: 588 3584
NgoMIV G'CCGG_C
Cuts at: 2351 2351
Size: 4172
NspI r_CATG'y
Cuts at: 1225 1725 2632 1225
Size: 500 907 2765
Fragments arranged by size:
2765 907 500
PciI A'CATG_T
Cuts at: 2628 2628
Size: 4172
PmeI GTTT'AAAC
Cuts at: 1171 1171
Size: 4172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3311 3343 3311
Size: 32 4140
PpuMI rG'GwC_Cy
Cuts at: 228 1089 228
Size: 861 3311
PshAI GACnn'nnGTC
Cuts at: 1087 1087
Size: 4172
PspOMI G'GGCC_C
Cuts at: 503 1076 503
Size: 573 3599
PstI C_TGCA'G
Cuts at: 1219 1904 1219
Size: 685 3487
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4172
PvuII CAG'CTG
Cuts at: 992 1597 1957 992
Size: 605 360 3207
Fragments arranged by size:
3207 605 360
RsrII CG'GwC_CG
Cuts at: 2367 2367
Size: 4172
SacI G_AGCT'C
Cuts at: 1186 1186
Size: 4172
SalI G'TCGA_C
Cuts at: 1209 1209
Size: 4172
SapI GCTCTTCn'nnn_
Cuts at: 2195 2405 2195
Size: 210 3962
SbfI CC_TGCA'GG
Cuts at: 1219 1219
Size: 4172
ScaI AGT'ACT
Cuts at: 612 3689 612
Size: 3077 1095
SfcI C'TryA_G
Cuts at: 33 668 1215 1900 2893 3084 33
Size: 635 547 685 993 191 1121
Fragments arranged by size:
1121 993 685 635 547 191
SfoI GGC'GCC
Cuts at: 1604 1852 1604
Size: 248 3924
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4172
SmaI CCC'GGG
Cuts at: 1194 1194
Size: 4172
SmlI C'TyrA_G
Cuts at: 1327 2242 2734 2996 1327
Size: 915 492 262 2503
Fragments arranged by size:
2503 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1063 67
Size: 996 3176
SphI G_CATG'C
Cuts at: 1225 1725 1225
Size: 500 3672
StyI C'CwwG_G
Cuts at: 107 695 701 974 1306 1660 107
Size: 588 6 273 332 354 2619
Fragments arranged by size:
2619 588 354 332 273 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 243 1755 1888 2347 2556 243
Size: 1512 133 459 209 1859
Fragments arranged by size:
1859 1512 459 209 133
TatI w'GTAC_w
Cuts at: 610 3687 610
Size: 3077 1095
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3689 3721 3689
Size: 32 4140
Tth111I GACn'n_nGTC
Cuts at: 1969 1969
Size: 4172
XbaI T'CTAG_A
Cuts at: 1203 1203
Size: 4172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 465 727 1667 465
Size: 262 940 2970
Fragments arranged by size:
2970 940 262
XmaI C'CCGG_G
Cuts at: 1192 1192
Size: 4172
XmnI GAAnn'nnTTC
Cuts at: 685 685
Size: 4172
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BsgI BsiWI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI