Restriction map for
pF1KB7150
(Circular) (Six-base) MAPSORT of: pF1KB7150.seq Check: 4249 from: 1 to: 4154
pF1KB7150 4154 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1027 1192 1027
Size: 165 3989
Acc65I G'GTAC_C
Cuts at: 1170 1170
Size: 4154
AclI AA'CG_TT
Cuts at: 3994 3994
Size: 4154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 1979 2411 3158 3426 631
Size: 1348 432 747 268 1359
Fragments arranged by size:
1359 1348 747 432 268
AflIII A'CryG_T
Cuts at: 1506 2610 3587 1506
Size: 1104 977 2073
Fragments arranged by size:
2073 1104 977
AgeI A'CCGG_T
Cuts at: 2542 2542
Size: 4154
AlwNI CAG_nnn'CTG
Cuts at: 1459 3026 1459
Size: 1567 2587
ApaI G_GGCC'C
Cuts at: 937 937
Size: 4154
ApaLI G'TGCA_C
Cuts at: 1106 2924 1106
Size: 1818 2336
ApoI r'AATT_y
Cuts at: 1012 1158 3507 3763 1012
Size: 146 2349 256 1403
Fragments arranged by size:
2349 1403 256 146
AseI AT'TA_AT
Cuts at: 20 20
Size: 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
AvaI C'yCGr_G
Cuts at: 113 161 1174 113
Size: 48 1013 3093
Fragments arranged by size:
3093 1013 48
BamHI G'GATC_C
Cuts at: 1179 1179
Size: 4154
BanI G'GyrC_C
Cuts at: 1170 1584 1832 1867 1170
Size: 414 248 35 3457
Fragments arranged by size:
3457 414 248 35
BanII G_rGCy'C
Cuts at: 500 839 937 945 1168 2198 500
Size: 339 98 8 223 1030 2456
Fragments arranged by size:
2456 1030 339 223 98 8
BbeI G_GCGC'C
Cuts at: 1588 1836 1588
Size: 248 3906
BbvCI CC'TCA_GC
Cuts at: 764 764
Size: 4154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 741 775 90
Size: 34 617 34 3469
Fragments arranged by size:
3469 617 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2052 2813 4154 2052
Size: 761 1341 2052
Fragments arranged by size:
2052 1341 761
BclI T'GATC_A
Cuts at: 1674 1674
Size: 4154
BglII A'GATC_T
Cuts at: 402 660 1669 3706 402
Size: 258 1009 2037 850
Fragments arranged by size:
2037 1009 850 258
BlpI GC'TnA_GC
Cuts at: 1266 1266
Size: 4154
Bme1580I G_kGCm'C
Cuts at: 704 899 937 1110 1779 1872 2928 704
Size: 195 38 173 669 93 1056 1930
Fragments arranged by size:
1930 1056 669 195 173 93 38
BmrI ACTGGGnnnn_n'
Cuts at: 1001 1366 1447 1782 3926 1001
Size: 365 81 335 2144 1229
Fragments arranged by size:
2144 1229 365 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2521 2521
Size: 4154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 901 607
Size: 294 3860
Bpu10I CC'TnA_GC
Cuts at: 565 764 565
Size: 199 3955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 1330 2209 2701 2999 994
Size: 336 879 492 298 2149
Fragments arranged by size:
2149 879 492 336 298
BsaI GGTCTCn'nnnn_
Cuts at: 3858 3858
Size: 4154
BsaAI yAC'GTr
Cuts at: 1507 1507
Size: 4154
BsaBI GATnn'nnATC
Cuts at: 6 1668 3705 6
Size: 1662 2037 455
Fragments arranged by size:
2037 1662 455
BsaHI Gr'CG_yC
Cuts at: 1585 1833 2535 4031 1585
Size: 248 702 1496 1708
Fragments arranged by size:
1708 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1343 1864 2542 2816 2963 3374 1343
Size: 521 678 274 147 411 2123
Fragments arranged by size:
2123 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 179 223 781 179
Size: 44 558 3552
Fragments arranged by size:
3552 558 44
BseYI C'CCAG_C
Cuts at: 1579 2337 2914 3410 1579
Size: 758 577 496 2323
Fragments arranged by size:
2323 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1696 1742 2950 104
Size: 1592 46 1208 1308
Fragments arranged by size:
1592 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 584 821 1110 1168 1946 2136 2928 584
Size: 237 289 58 778 190 792 1810
Fragments arranged by size:
1810 792 778 289 237 190 58
Bsp1286I G_dGCh'C
Cuts at: 500 584 704 821 839 899 937 945 1110
Size: 84 120 117 18 60 38 8 165
Cuts at: 1110 1168 1779 1872 1946 2136 2198 2928 500
Size: 58 611 93 74 190 62 730 1726
Fragments arranged by size:
1726 730 611 190 165 120 117 93
84 74 62 60 58 38 18 8
BspEI T'CCGG_A
Cuts at: 3374 3374
Size: 4154
BspHI T'CATG_A
Cuts at: 3330 3330
Size: 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1137 1204 1418 1720 2101 1137
Size: 67 214 302 381 3190
Fragments arranged by size:
3190 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2446 2500 3984 4149 50
Size: 2396 54 1484 165 55
Fragments arranged by size:
2396 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2066 2066
Size: 4154
BsrFI r'CCGG_y
Cuts at: 1102 2152 2333 2542 1102
Size: 1050 181 209 2714
Fragments arranged by size:
2714 1050 209 181
BsrGI T'GTAC_A
Cuts at: 476 476
Size: 4154
BssSI C'ACGA_G
Cuts at: 2425 2783 2425
Size: 358 3796
BstAPI GCAn_nnn'nTGC
Cuts at: 471 1531 471
Size: 1060 3094
BstBI TT'CG_AA
Cuts at: 2515 2515
Size: 4154
BstXI CCAn_nnnn'nTGG
Cuts at: 861 861
Size: 4154
BstYI r'GATC_y
Cuts at: 1 402 660 1179 1646 1669 2004 2250 3262
Size: 401 258 519 467 23 335 246 1012
Cuts at: 3262 3348 3360 3706 1
Size: 86 12 346 449
Fragments arranged by size:
1012 519 467 449 401 346 335 258
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1028 1028
Size: 4154
BtgI C'CryG_G
Cuts at: 291 291
Size: 4154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2104 2285 2448 2104
Size: 181 163 3810
Fragments arranged by size:
3810 181 163
BtsI GCAGTG_nn'
Cuts at: 1397 1397
Size: 4154
DraI TTT'AAA
Cuts at: 1153 1153
Size: 4154
DrdI GACnn_nn'nnGTC
Cuts at: 1860 2718 1860
Size: 858 3296
EaeI y'GGCC_r
Cuts at: 983 1739 1913 2304 2331 3676 983
Size: 756 174 391 27 1345 1461
Fragments arranged by size:
1461 1345 756 391 174 27
EagI C'GGCC_G
Cuts at: 1739 1739
Size: 4154
EarI CTCTTCn'nnn_
Cuts at: 118 200 1046 2177 2387 118
Size: 82 846 1131 210 1885
Fragments arranged by size:
1885 1131 846 210 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2362 2672 2818 3964 2362
Size: 310 146 1146 2552
Fragments arranged by size:
2552 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1166 1166
Size: 4154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 631 901 1979 2411 3158 3426 607
Size: 24 270 1078 432 747 268 1335
Fragments arranged by size:
1335 1078 747 432 270 268 24
EcoO109I rG'GnC_Cy
Cuts at: 433 933 1293 433
Size: 500 360 3294
Fragments arranged by size:
3294 500 360
EcoRI G'AATT_C
Cuts at: 1012 1158 1012
Size: 146 4008
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 4154
FspI TGC'GCA
Cuts at: 1935 1935
Size: 4154
HaeII r_GCGC'y
Cuts at: 1588 1836 2858 3837 1588
Size: 248 1022 979 1905
Fragments arranged by size:
1905 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 174 202 206 234 262 294 585 617 1996
Size: 28 4 28 28 32 291 32 1379
Cuts at: 1996 2026 2028 2058 174
Size: 30 2 30 2270
Fragments arranged by size:
2270 1379 291 32 32 30 30 28
28 28 4 2
HincII GTy'rAC
Cuts at: 366 651 1193 366
Size: 285 542 3327
Fragments arranged by size:
3327 542 285
KasI G'GCGC_C
Cuts at: 1584 1832 1584
Size: 248 3906
KpnI G_GTAC'C
Cuts at: 1174 1174
Size: 4154
MluI A'CGCG_T
Cuts at: 3587 3587
Size: 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 305 2825 3009 305
Size: 2520 184 1450
Fragments arranged by size:
2520 1450 184
MscI TGG'CCA
Cuts at: 985 1915 985
Size: 930 3224
MslI CAynn'nnrTG
Cuts at: 638 2143 2270 638
Size: 1505 127 2522
Fragments arranged by size:
2522 1505 127
MspA1I CmG'CkG
Cuts at: 334 1265 1579 1939 2952 3197 334
Size: 931 314 360 1013 245 1291
Fragments arranged by size:
1291 1013 931 360 314 245
NaeI GCC'GGC
Cuts at: 2335 2335
Size: 4154
NarI GG'CG_CC
Cuts at: 1585 1833 1585
Size: 248 3906
NcoI C'CATG_G
Cuts at: 291 291
Size: 4154
NgoMIV G'CCGG_C
Cuts at: 2333 2333
Size: 4154
NspI r_CATG'y
Cuts at: 1207 1707 2614 1207
Size: 500 907 2747
Fragments arranged by size:
2747 907 500
PciI A'CATG_T
Cuts at: 2610 2610
Size: 4154
PmeI GTTT'AAAC
Cuts at: 1153 1153
Size: 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 4122
PspOMI G'GGCC_C
Cuts at: 933 933
Size: 4154
PspXI vC'TCGA_Gb
Cuts at: 113 113
Size: 4154
PstI C_TGCA'G
Cuts at: 1148 1201 1886 1148
Size: 53 685 3416
Fragments arranged by size:
3416 685 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4154
PvuII CAG'CTG
Cuts at: 334 1579 1939 334
Size: 1245 360 2549
Fragments arranged by size:
2549 1245 360
RsrII CG'GwC_CG
Cuts at: 2349 2349
Size: 4154
SacI G_AGCT'C
Cuts at: 1168 1168
Size: 4154
SalI G'TCGA_C
Cuts at: 1191 1191
Size: 4154
SapI GCTCTTCn'nnn_
Cuts at: 2177 2387 2177
Size: 210 3944
SbfI CC_TGCA'GG
Cuts at: 1201 1201
Size: 4154
ScaI AGT'ACT
Cuts at: 3671 3671
Size: 4154
SfcI C'TryA_G
Cuts at: 33 329 437 1144 1197 1882 2875 3066 33
Size: 296 108 707 53 685 993 191 1121
Fragments arranged by size:
1121 993 707 685 296 191 108 53
SfoI GGC'GCC
Cuts at: 1586 1834 1586
Size: 248 3906
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
SmaI CCC'GGG
Cuts at: 1176 1176
Size: 4154
SmlI C'TyrA_G
Cuts at: 113 973 1309 2224 2716 2978 113
Size: 860 336 915 492 262 1289
Fragments arranged by size:
1289 915 860 492 336 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4154
SphI G_CATG'C
Cuts at: 1207 1707 1207
Size: 500 3654
StyI C'CwwG_G
Cuts at: 291 986 1079 1115 1288 1642 291
Size: 695 93 36 173 354 2803
Fragments arranged by size:
2803 695 354 173 93 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1737 1870 2329 2538 1737
Size: 133 459 209 3353
Fragments arranged by size:
3353 459 209 133
TatI w'GTAC_w
Cuts at: 476 3669 476
Size: 3193 961
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 336 368 3671 3703 336
Size: 32 3303 32 787
Fragments arranged by size:
3303 787 32 32
Tth111I GACn'n_nGTC
Cuts at: 1951 1951
Size: 4154
XbaI T'CTAG_A
Cuts at: 405 1185 405
Size: 780 3374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1649 1649
Size: 4154
XhoI C'TCGA_G
Cuts at: 113 113
Size: 4154
XmaI C'CCGG_G
Cuts at: 1174 1174
Size: 4154
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BfrBI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI