Restriction map for
pF1KB7147
(Circular) (Six-base) MAPSORT of: pF1KB7147.seq Check: 8566 from: 1 to: 4151
pF1KB7147 4151 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 557 920 932 1189 557
Size: 363 12 257 3519
Fragments arranged by size:
3519 363 257 12
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1976 2408 3155 3423 1054
Size: 922 432 747 268 1782
Fragments arranged by size:
1782 922 747 432 268
AflIII A'CryG_T
Cuts at: 833 1503 2607 3584 833
Size: 670 1104 977 1400
Fragments arranged by size:
1400 1104 977 670
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 713 1456 3023 713
Size: 743 1567 1841
Fragments arranged by size:
1841 1567 743
ApaI G_GGCC'C
Cuts at: 827 827
Size: 4151
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 568 1155 3504 3760 568
Size: 587 2349 256 959
Fragments arranged by size:
2349 959 587 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 1171 1171
Size: 4151
BamHI G'GATC_C
Cuts at: 734 1176 734
Size: 442 3709
BanI G'GyrC_C
Cuts at: 598 1167 1581 1829 1864 598
Size: 569 414 248 35 2885
Fragments arranged by size:
2885 569 414 248 35
BanII G_rGCy'C
Cuts at: 194 827 998 1165 2195 194
Size: 633 171 167 1030 2150
Fragments arranged by size:
2150 1030 633 171 167
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BbsI GAAGACnn'nnnn_
Cuts at: 150 213 150
Size: 63 4088
BbvCI CC'TCA_GC
Cuts at: 979 979
Size: 4151
BciVI GTATCCnnnnn_n'
Cuts at: 105 2049 2810 4151 105
Size: 1944 761 1341 105
Fragments arranged by size:
1944 1341 761 105
BclI T'GATC_A
Cuts at: 698 1671 698
Size: 973 3178
BglI GCCn_nnn'nGGC
Cuts at: 660 660
Size: 4151
BglII A'GATC_T
Cuts at: 1666 3703 1666
Size: 2037 2114
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 601 827 1776 1869 2925 601
Size: 226 949 93 1056 1827
Fragments arranged by size:
1827 1056 949 226 93
BmgBI CAC'GTC
Cuts at: 261 261
Size: 4151
BmrI ACTGGGnnnn_n'
Cuts at: 1363 1444 1779 3923 1363
Size: 81 335 2144 1591
Fragments arranged by size:
2144 1591 335 81
BmtI G_CTAG'C
Cuts at: 643 643
Size: 4151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 685 640
Size: 45 4106
Bpu10I CC'TnA_GC
Cuts at: 979 979
Size: 4151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 2206 2698 2996 1327
Size: 879 492 298 2482
Fragments arranged by size:
2482 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3855 3855
Size: 4151
BsaAI yAC'GTr
Cuts at: 888 1504 888
Size: 616 3535
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 1582 1830 2532 4028 1582
Size: 248 702 1496 1705
Fragments arranged by size:
1705 1496 702 248
BsaWI w'CCGG_w
Cuts at: 626 1340 1861 2539 2813 2960 3371 626
Size: 714 521 678 274 147 411 1406
Fragments arranged by size:
1406 714 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 925 928 955 958 975 1005 925
Size: 3 27 3 17 30 4071
Fragments arranged by size:
4071 30 27 17 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 783 933 936 996 783
Size: 150 3 60 3938
Fragments arranged by size:
3938 150 60 3
BseYI C'CCAG_C
Cuts at: 492 1576 2334 2911 3407 492
Size: 1084 758 577 496 1236
Fragments arranged by size:
1236 1084 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 487 1693 1739 2947 104
Size: 383 1206 46 1208 1308
Fragments arranged by size:
1308 1208 1206 383 46
BsiHKAI G_wGCw'C
Cuts at: 266 1165 1943 2133 2925 266
Size: 899 778 190 792 1492
Fragments arranged by size:
1492 899 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 194 266 601 827 998 1165 1776 1869 1943
Size: 72 335 226 171 167 611 93 74
Cuts at: 1943 2133 2195 2925 194
Size: 190 62 730 1420
Fragments arranged by size:
1420 730 611 335 226 190 171 167
93 74 72 62
BspEI T'CCGG_A
Cuts at: 626 3371 626
Size: 2745 1406
BspHI T'CATG_A
Cuts at: 969 3327 969
Size: 2358 1793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 212 299 493 619 1201 1415 1717 2098 212
Size: 87 194 126 582 214 302 381 2265
Fragments arranged by size:
2265 582 381 302 214 194 126 87
BsrBI CCG'CTC
Cuts at: 50 1066 2443 2497 3981 4146 50
Size: 1016 1377 54 1484 165 55
Fragments arranged by size:
1484 1377 1016 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2063 2063
Size: 4151
BsrFI r'CCGG_y
Cuts at: 761 2149 2330 2539 761
Size: 1388 181 209 2373
Fragments arranged by size:
2373 1388 209 181
BssSI C'ACGA_G
Cuts at: 2422 2780 2422
Size: 358 3793
BstAPI GCAn_nnn'nTGC
Cuts at: 1528 1528
Size: 4151
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstYI r'GATC_y
Cuts at: 1 734 881 1176 1643 1666 2001 2247 3259
Size: 733 147 295 467 23 335 246 1012
Cuts at: 3259 3345 3357 3703 1
Size: 86 12 346 449
Fragments arranged by size:
1012 733 467 449 346 335 295 246
147 86 23 12
BtgI C'CryG_G
Cuts at: 107 254 107
Size: 147 4004
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 543 1394 543
Size: 851 3300
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 928 1857 2715 928
Size: 929 858 2364
Fragments arranged by size:
2364 929 858
EaeI y'GGCC_r
Cuts at: 251 484 791 1122 1736 1910 2301 2328 3673
Size: 233 307 331 614 174 391 27 1345
Cuts at: 3673 251
Size: 729
Fragments arranged by size:
1345 729 614 391 331 307 233 174
27
EagI C'GGCC_G
Cuts at: 484 1736 484
Size: 1252 2899
EarI CTCTTCn'nnn_
Cuts at: 424 1049 2174 2384 424
Size: 625 1125 210 2191
Fragments arranged by size:
2191 1125 625 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2359 2669 2815 3961 2359
Size: 310 146 1146 2549
Fragments arranged by size:
2549 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1163 1163
Size: 4151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 685 1054 1976 2408 3155 3423 640
Size: 45 369 922 432 747 268 1368
Fragments arranged by size:
1368 922 747 432 369 268 45
EcoO109I rG'GnC_Cy
Cuts at: 118 608 824 1290 118
Size: 490 216 466 2979
Fragments arranged by size:
2979 490 466 216
EcoRI G'AATT_C
Cuts at: 568 1155 568
Size: 587 3564
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 4151
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 243 1585 1833 2855 3834 243
Size: 1342 248 1022 979 560
Fragments arranged by size:
1342 1022 979 560 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1993 2023 2025 2055 1993
Size: 30 2 30 4089
Fragments arranged by size:
4089 30 30 2
HincII GTy'rAC
Cuts at: 921 1190 921
Size: 269 3882
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 833 3584 833
Size: 2751 1400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2822 3006 2822
Size: 184 3967
MscI TGG'CCA
Cuts at: 1124 1912 1124
Size: 788 3363
MslI CAynn'nnrTG
Cuts at: 2140 2267 2140
Size: 127 4024
MspA1I CmG'CkG
Cuts at: 430 696 1078 1262 1576 1936 2949 3194 430
Size: 266 382 184 314 360 1013 245 1387
Fragments arranged by size:
1387 1013 382 360 314 266 245 184
NaeI GCC'GGC
Cuts at: 763 2332 763
Size: 1569 2582
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NcoI C'CATG_G
Cuts at: 107 107
Size: 4151
NgoMIV G'CCGG_C
Cuts at: 761 2330 761
Size: 1569 2582
NheI G'CTAG_C
Cuts at: 639 639
Size: 4151
NspI r_CATG'y
Cuts at: 345 1204 1704 2611 345
Size: 859 500 907 1885
Fragments arranged by size:
1885 907 859 500
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PflMI CCAn_nnn'nTGG
Cuts at: 379 846 379
Size: 467 3684
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PpuMI rG'GwC_Cy
Cuts at: 608 608
Size: 4151
PspOMI G'GGCC_C
Cuts at: 823 823
Size: 4151
PstI C_TGCA'G
Cuts at: 239 550 1198 1883 239
Size: 311 648 685 2507
Fragments arranged by size:
2507 685 648 311
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 430 1078 1576 1936 430
Size: 648 498 360 2645
Fragments arranged by size:
2645 648 498 360
RsrII CG'GwC_CG
Cuts at: 1068 2346 1068
Size: 1278 2873
SacI G_AGCT'C
Cuts at: 1165 1165
Size: 4151
SalI G'TCGA_C
Cuts at: 919 1188 919
Size: 269 3882
SapI GCTCTTCn'nnn_
Cuts at: 1049 2174 2384 1049
Size: 1125 210 2816
Fragments arranged by size:
2816 1125 210
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 3668 3668
Size: 4151
SfcI C'TryA_G
Cuts at: 33 235 546 717 1194 1879 2872 3063 33
Size: 202 311 171 477 685 993 191 1121
Fragments arranged by size:
1121 993 685 477 311 202 191 171
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1173 1173
Size: 4151
SmlI C'TyrA_G
Cuts at: 1306 2221 2713 2975 1306
Size: 915 492 262 2482
Fragments arranged by size:
2482 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 345 1204 1704 345
Size: 859 500 2792
Fragments arranged by size:
2792 859 500
StyI C'CwwG_G
Cuts at: 107 1285 1639 107
Size: 1178 354 2619
Fragments arranged by size:
2619 1178 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 479 1085 1734 1867 2326 2535 479
Size: 606 649 133 459 209 2095
Fragments arranged by size:
2095 649 606 459 209 133
TatI w'GTAC_w
Cuts at: 3666 3666
Size: 4151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 975 1007 3668 3700 975
Size: 32 2661 32 1426
Fragments arranged by size:
2661 1426 32 32
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 601 1646 601
Size: 1045 3106
XmaI C'CCGG_G
Cuts at: 1171 1171
Size: 4151
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI