Restriction map for
pF1KB7146
(Circular) (Six-base) MAPSORT of: pF1KB7146.seq Check: 903 from: 1 to: 4151
pF1KB7146 4151 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1189 1189
Size: 4151
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1976 2408 3155 3423 1976
Size: 432 747 268 2704
Fragments arranged by size:
2704 747 432 268
AflIII A'CryG_T
Cuts at: 1503 2607 3584 1503
Size: 1104 977 2070
Fragments arranged by size:
2070 1104 977
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AhdI GACnn_n'nnGTC
Cuts at: 164 164
Size: 4151
AleI CACnn'nnGTG
Cuts at: 185 185
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 471 1456 3023 471
Size: 985 1567 1599
Fragments arranged by size:
1599 1567 985
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 781 922 1155 3504 3760 781
Size: 141 233 2349 256 1172
Fragments arranged by size:
2349 1172 256 233 141
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 113 161 1171 113
Size: 48 1010 3093
Fragments arranged by size:
3093 1010 48
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 1167 1581 1829 1864 1167
Size: 414 248 35 3454
Fragments arranged by size:
3454 414 248 35
BanII G_rGCy'C
Cuts at: 839 945 1165 2195 839
Size: 106 220 1030 2795
Fragments arranged by size:
2795 1030 220 106
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BbsI GAAGACnn'nnnn_
Cuts at: 454 454
Size: 4151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4117
BciVI GTATCCnnnnn_n'
Cuts at: 231 2049 2810 4151 231
Size: 1818 761 1341 231
Fragments arranged by size:
1818 1341 761 231
BclI T'GATC_A
Cuts at: 1671 1671
Size: 4151
BglII A'GATC_T
Cuts at: 773 1666 3703 773
Size: 893 2037 1221
Fragments arranged by size:
2037 1221 893
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 899 1776 1869 2925 899
Size: 877 93 1056 2125
Fragments arranged by size:
2125 1056 877 93
BmrI ACTGGGnnnn_n'
Cuts at: 1001 1363 1444 1779 3923 1001
Size: 362 81 335 2144 1229
Fragments arranged by size:
2144 1229 362 335 81
BmtI G_CTAG'C
Cuts at: 1130 1130
Size: 4151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 994 901
Size: 93 4058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1327 2206 2698 2996 277
Size: 1050 879 492 298 1432
Fragments arranged by size:
1432 1050 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 718 3855 718
Size: 3137 1014
BsaAI yAC'GTr
Cuts at: 142 283 1504 142
Size: 141 1221 2789
Fragments arranged by size:
2789 1221 141
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 1582 1830 2532 4028 1582
Size: 248 702 1496 1705
Fragments arranged by size:
1705 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2813 2960 3371 1340
Size: 521 678 274 147 411 2120
Fragments arranged by size:
2120 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 580 610 580
Size: 30 4121
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 781 781
Size: 4151
BseYI C'CCAG_C
Cuts at: 1576 2334 2911 3407 1576
Size: 758 577 496 2320
Fragments arranged by size:
2320 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 484 896 439
Size: 45 412 3694
Fragments arranged by size:
3694 412 45
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2947 104
Size: 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 192 291 312 1165 1943 2133 2925 192
Size: 99 21 853 778 190 792 1418
Fragments arranged by size:
1418 853 792 778 190 99 21
Bsp1286I G_dGCh'C
Cuts at: 192 291 312 839 899 945 1165 1776 1869
Size: 99 21 527 60 46 220 611 93
Cuts at: 1869 1943 2133 2195 2925 192
Size: 74 190 62 730 1418
Fragments arranged by size:
1418 730 611 527 220 190 99 93
74 62 60 46 21
BspEI T'CCGG_A
Cuts at: 3371 3371
Size: 4151
BspHI T'CATG_A
Cuts at: 743 3327 743
Size: 2584 1567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1201 1415 1717 2098 1201
Size: 214 302 381 3254
Fragments arranged by size:
3254 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2443 2497 3981 4146 50
Size: 2393 54 1484 165 55
Fragments arranged by size:
2393 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2063 2063
Size: 4151
BsrFI r'CCGG_y
Cuts at: 2149 2330 2539 2149
Size: 181 209 3761
Fragments arranged by size:
3761 209 181
BsrGI T'GTAC_A
Cuts at: 482 482
Size: 4151
BssSI C'ACGA_G
Cuts at: 686 2422 2780 686
Size: 1736 358 2057
Fragments arranged by size:
2057 1736 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1528 1528
Size: 4151
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstYI r'GATC_y
Cuts at: 1 560 660 773 1176 1643 1666 2001 2247
Size: 559 100 113 403 467 23 335 246
Cuts at: 2247 3259 3345 3357 3703 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 559 467 449 403 346 335 246
113 100 86 23 12
BtgI C'CryG_G
Cuts at: 1097 1097
Size: 4151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 197 1394 197
Size: 1197 2954
DraI TTT'AAA
Cuts at: 611 1150 611
Size: 539 3612
DraIII CAC_nnn'GTG
Cuts at: 286 286
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 640 1857 2715 640
Size: 1217 858 2076
Fragments arranged by size:
2076 1217 858
EaeI y'GGCC_r
Cuts at: 1736 1910 2301 2328 3673 1736
Size: 174 391 27 1345 2214
Fragments arranged by size:
2214 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 118 1025 1140 2174 2384 118
Size: 907 115 1034 210 1885
Fragments arranged by size:
1885 1034 907 210 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2359 2669 2815 3961 2359
Size: 310 146 1146 2549
Fragments arranged by size:
2549 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1163 1163
Size: 4151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 994 1976 2408 3155 3423 901
Size: 93 982 432 747 268 1629
Fragments arranged by size:
1629 982 747 432 268 93
EcoO109I rG'GnC_Cy
Cuts at: 969 1290 969
Size: 321 3830
EcoRI G'AATT_C
Cuts at: 781 922 1155 781
Size: 141 233 3777
Fragments arranged by size:
3777 233 141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 408 567 837 2170 408
Size: 159 270 1333 2389
Fragments arranged by size:
2389 1333 270 159
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 1585 1833 2855 3834 1585
Size: 248 1022 979 1902
Fragments arranged by size:
1902 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 150 162 182 194 669 701 1993 2023 2025
Size: 12 20 12 475 32 1292 30 2
Cuts at: 2025 2055 150
Size: 30 2246
Fragments arranged by size:
2246 1292 475 32 30 30 20 12
12 2
HincII GTy'rAC
Cuts at: 1190 1190
Size: 4151
HindIII A'AGCT_T
Cuts at: 390 572 868 390
Size: 182 296 3673
Fragments arranged by size:
3673 296 182
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2822 3006 2822
Size: 184 3967
MscI TGG'CCA
Cuts at: 1912 1912
Size: 4151
MslI CAynn'nnrTG
Cuts at: 140 185 589 2140 2267 140
Size: 45 404 1551 127 2024
Fragments arranged by size:
2024 1551 404 127 45
MspA1I CmG'CkG
Cuts at: 1262 1576 1936 2949 3194 1262
Size: 314 360 1013 245 2219
Fragments arranged by size:
2219 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NcoI C'CATG_G
Cuts at: 1097 1097
Size: 4151
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NheI G'CTAG_C
Cuts at: 1126 1126
Size: 4151
NspI r_CATG'y
Cuts at: 1204 1704 2611 1204
Size: 500 907 2744
Fragments arranged by size:
2744 907 500
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PflMI CCAn_nnn'nTGG
Cuts at: 861 861
Size: 4151
PfoI T'CCnGG_A
Cuts at: 971 971
Size: 4151
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PmlI CAC'GTG
Cuts at: 142 142
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PpuMI rG'GwC_Cy
Cuts at: 969 969
Size: 4151
PstI C_TGCA'G
Cuts at: 1071 1198 1883 1071
Size: 127 685 3339
Fragments arranged by size:
3339 685 127
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 1576 1936 1576
Size: 360 3791
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 1165 1165
Size: 4151
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 1140 2174 2384 1140
Size: 1034 210 2907
Fragments arranged by size:
2907 1034 210
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 3668 3668
Size: 4151
SfcI C'TryA_G
Cuts at: 33 329 1067 1194 1879 2872 3063 33
Size: 296 738 127 685 993 191 1121
Fragments arranged by size:
1121 993 738 685 296 191 127
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 163 1173 163
Size: 1010 3141
SmlI C'TyrA_G
Cuts at: 292 1306 2221 2713 2975 292
Size: 1014 915 492 262 1468
Fragments arranged by size:
1468 1014 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
StyI C'CwwG_G
Cuts at: 629 986 1097 1115 1121 1285 1639 629
Size: 357 111 18 6 164 354 3141
Fragments arranged by size:
3141 357 354 164 111 18 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 868 1734 1867 2326 2535 868
Size: 866 133 459 209 2484
Fragments arranged by size:
2484 866 459 209 133
TatI w'GTAC_w
Cuts at: 482 3666 482
Size: 3184 967
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4119
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1646 1646
Size: 4151
XmaI C'CCGG_G
Cuts at: 161 1171 161
Size: 1010 3141
XmnI GAAnn'nnTTC
Cuts at: 550 820 1110 550
Size: 270 290 3591
Fragments arranged by size:
3591 290 270
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI Bpu10I BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI