Restriction map for
pF1KB7142
(Circular) (Six-base) MAPSORT of: pF1KB7142.seq Check: 7734 from: 1 to: 4124
pF1KB7142 4124 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1162 1162
Size: 4124
Acc65I G'GTAC_C
Cuts at: 1140 1140
Size: 4124
AclI AA'CG_TT
Cuts at: 3964 3964
Size: 4124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 1949 2381 3128 3396 423
Size: 1526 432 747 268 1151
Fragments arranged by size:
1526 1151 747 432 268
AflII C'TTAA_G
Cuts at: 811 811
Size: 4124
AflIII A'CryG_T
Cuts at: 1476 2580 3557 1476
Size: 1104 977 2043
Fragments arranged by size:
2043 1104 977
AgeI A'CCGG_T
Cuts at: 2512 2512
Size: 4124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 361 361
Size: 4124
AlwNI CAG_nnn'CTG
Cuts at: 1429 2996 1429
Size: 1567 2557
ApaLI G'TGCA_C
Cuts at: 2894 2894
Size: 4124
ApoI r'AATT_y
Cuts at: 1128 3477 3733 1128
Size: 2349 256 1519
Fragments arranged by size:
2349 1519 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
AvaI C'yCGr_G
Cuts at: 1144 1144
Size: 4124
BamHI G'GATC_C
Cuts at: 1149 1149
Size: 4124
BanI G'GyrC_C
Cuts at: 1140 1554 1802 1837 1140
Size: 414 248 35 3427
Fragments arranged by size:
3427 414 248 35
BanII G_rGCy'C
Cuts at: 1138 2168 1138
Size: 1030 3094
BbeI G_GCGC'C
Cuts at: 1558 1806 1558
Size: 248 3876
BciVI GTATCCnnnnn_n'
Cuts at: 2022 2783 4124 2022
Size: 761 1341 2022
Fragments arranged by size:
2022 1341 761
BclI T'GATC_A
Cuts at: 509 620 1644 509
Size: 111 1024 2989
Fragments arranged by size:
2989 1024 111
BglII A'GATC_T
Cuts at: 342 1639 3676 342
Size: 1297 2037 790
Fragments arranged by size:
2037 1297 790
BlpI GC'TnA_GC
Cuts at: 1236 1236
Size: 4124
Bme1580I G_kGCm'C
Cuts at: 1749 1842 2898 1749
Size: 93 1056 2975
Fragments arranged by size:
2975 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1336 1417 1752 3896 1336
Size: 81 335 2144 1564
Fragments arranged by size:
2144 1564 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 4124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 906 1102 1300 2179 2671 2969 184
Size: 722 196 198 879 492 298 1339
Fragments arranged by size:
1339 879 722 492 298 198 196
BsaI GGTCTCn'nnnn_
Cuts at: 288 3828 288
Size: 3540 584
BsaAI yAC'GTr
Cuts at: 861 1477 861
Size: 616 3508
BsaBI GATnn'nnATC
Cuts at: 6 1638 3675 6
Size: 1632 2037 455
Fragments arranged by size:
2037 1632 455
BsaHI Gr'CG_yC
Cuts at: 1555 1803 2505 4001 1555
Size: 248 702 1496 1678
Fragments arranged by size:
1678 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1313 1834 2512 2786 2933 3344 1313
Size: 521 678 274 147 411 2093
Fragments arranged by size:
2093 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1549 2307 2884 3380 1549
Size: 758 577 496 2293
Fragments arranged by size:
2293 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 575 321
Size: 254 3870
BsiEI CG_ry'CG
Cuts at: 104 1666 1712 2920 104
Size: 1562 46 1208 1308
Fragments arranged by size:
1562 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1138 1916 2106 2898 1138
Size: 778 190 792 2364
Fragments arranged by size:
2364 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1138 1749 1842 1916 2106 2168 2898 1138
Size: 611 93 74 190 62 730 2364
Fragments arranged by size:
2364 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3344 3344
Size: 4124
BspHI T'CATG_A
Cuts at: 3300 3300
Size: 4124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1174 1388 1690 2071 1174
Size: 214 302 381 3227
Fragments arranged by size:
3227 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2416 2470 3954 4119 50
Size: 2366 54 1484 165 55
Fragments arranged by size:
2366 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 264 2036 264
Size: 1772 2352
BsrFI r'CCGG_y
Cuts at: 2122 2303 2512 2122
Size: 181 209 3734
Fragments arranged by size:
3734 209 181
BssSI C'ACGA_G
Cuts at: 241 2395 2753 241
Size: 2154 358 1612
Fragments arranged by size:
2154 1612 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1501 1501
Size: 4124
BstBI TT'CG_AA
Cuts at: 2485 2485
Size: 4124
BstEII G'GTnAC_C
Cuts at: 997 997
Size: 4124
BstYI r'GATC_y
Cuts at: 1 342 404 944 1149 1616 1639 1974 2220
Size: 341 62 540 205 467 23 335 246
Cuts at: 2220 3232 3318 3330 3676 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 540 467 449 346 341 335 246
205 86 62 23 12
BtgI C'CryG_G
Cuts at: 786 786
Size: 4124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2074 2255 2418 2074
Size: 181 163 3780
Fragments arranged by size:
3780 181 163
BtsI GCAGTG_nn'
Cuts at: 1367 1367
Size: 4124
DraI TTT'AAA
Cuts at: 1123 1123
Size: 4124
DrdI GACnn_nn'nnGTC
Cuts at: 1830 2688 1830
Size: 858 3266
EaeI y'GGCC_r
Cuts at: 1709 1883 2274 2301 3646 1709
Size: 174 391 27 1345 2187
Fragments arranged by size:
2187 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1709 1709
Size: 4124
EarI CTCTTCn'nnn_
Cuts at: 298 478 2147 2357 298
Size: 180 1669 210 2065
Fragments arranged by size:
2065 1669 210 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2332 2642 2788 3934 2332
Size: 310 146 1146 2522
Fragments arranged by size:
2522 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1136 1136
Size: 4124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 1949 2381 3128 3396 423
Size: 1526 432 747 268 1151
Fragments arranged by size:
1526 1151 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1263 1263
Size: 4124
EcoRI G'AATT_C
Cuts at: 1128 1128
Size: 4124
EcoRV GAT'ATC
Cuts at: 272 272
Size: 4124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 4124
FspI TGC'GCA
Cuts at: 1905 1905
Size: 4124
HaeII r_GCGC'y
Cuts at: 1558 1806 2828 3807 1558
Size: 248 1022 979 1875
Fragments arranged by size:
1875 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 891 923 1966 1996 1998 2028 891
Size: 32 1043 30 2 30 2987
Fragments arranged by size:
2987 1043 32 30 30 2
HincII GTy'rAC
Cuts at: 1163 1163
Size: 4124
HindIII A'AGCT_T
Cuts at: 953 953
Size: 4124
KasI G'GCGC_C
Cuts at: 1554 1802 1554
Size: 248 3876
KpnI G_GTAC'C
Cuts at: 1144 1144
Size: 4124
MluI A'CGCG_T
Cuts at: 3557 3557
Size: 4124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2795 2979 2795
Size: 184 3940
MscI TGG'CCA
Cuts at: 1885 1885
Size: 4124
MslI CAynn'nnrTG
Cuts at: 319 383 607 2113 2240 319
Size: 64 224 1506 127 2203
Fragments arranged by size:
2203 1506 224 127 64
MspA1I CmG'CkG
Cuts at: 163 806 1235 1549 1909 2922 3167 163
Size: 643 429 314 360 1013 245 1120
Fragments arranged by size:
1120 1013 643 429 360 314 245
NaeI GCC'GGC
Cuts at: 2305 2305
Size: 4124
NarI GG'CG_CC
Cuts at: 1555 1803 1555
Size: 248 3876
NcoI C'CATG_G
Cuts at: 786 786
Size: 4124
NgoMIV G'CCGG_C
Cuts at: 2303 2303
Size: 4124
NspI r_CATG'y
Cuts at: 1177 1677 2584 1177
Size: 500 907 2717
Fragments arranged by size:
2717 907 500
PciI A'CATG_T
Cuts at: 2580 2580
Size: 4124
PmeI GTTT'AAAC
Cuts at: 1123 1123
Size: 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 4092
PstI C_TGCA'G
Cuts at: 1171 1856 1171
Size: 685 3439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4124
PvuII CAG'CTG
Cuts at: 163 806 1549 1909 163
Size: 643 743 360 2378
Fragments arranged by size:
2378 743 643 360
RsrII CG'GwC_CG
Cuts at: 2319 2319
Size: 4124
SacI G_AGCT'C
Cuts at: 1138 1138
Size: 4124
SalI G'TCGA_C
Cuts at: 1161 1161
Size: 4124
SapI GCTCTTCn'nnn_
Cuts at: 298 2147 2357 298
Size: 1849 210 2065
Fragments arranged by size:
2065 1849 210
SbfI CC_TGCA'GG
Cuts at: 1171 1171
Size: 4124
ScaI AGT'ACT
Cuts at: 3641 3641
Size: 4124
SfcI C'TryA_G
Cuts at: 33 1167 1852 2845 3036 33
Size: 1134 685 993 191 1121
Fragments arranged by size:
1134 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1556 1804 1556
Size: 248 3876
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
SmaI CCC'GGG
Cuts at: 1146 1146
Size: 4124
SmlI C'TyrA_G
Cuts at: 199 811 885 1081 1279 2194 2686 2948 199
Size: 612 74 196 198 915 492 262 1375
Fragments arranged by size:
1375 915 612 492 262 198 196 74
SnaBI TAC'GTA
Cuts at: 861 861
Size: 4124
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4124
SphI G_CATG'C
Cuts at: 1177 1677 1177
Size: 500 3624
StyI C'CwwG_G
Cuts at: 298 737 786 1258 1612 298
Size: 439 49 472 354 2810
Fragments arranged by size:
2810 472 439 354 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 533 1707 1840 2299 2508 533
Size: 1174 133 459 209 2149
Fragments arranged by size:
2149 1174 459 209 133
TatI w'GTAC_w
Cuts at: 3639 3639
Size: 4124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4092
Tth111I GACn'n_nGTC
Cuts at: 1921 1921
Size: 4124
XbaI T'CTAG_A
Cuts at: 967 1155 967
Size: 188 3936
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1619 1619
Size: 4124
XmaI C'CCGG_G
Cuts at: 1144 1144
Size: 4124
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI
ZraI