Restriction map for
pF1KB7139
(Circular) (Six-base) MAPSORT of: pF1KB7139.seq Check: 8955 from: 1 to: 4112
pF1KB7139 4112 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 806 806
Size: 4112
AccI GT'mk_AC
Cuts at: 380 1150 380
Size: 770 3342
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1937 2369 3116 3384 1937
Size: 432 747 268 2665
Fragments arranged by size:
2665 747 432 268
AflIII A'CryG_T
Cuts at: 1464 2568 3545 1464
Size: 1104 977 2031
Fragments arranged by size:
2031 1104 977
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AhdI GACnn_n'nnGTC
Cuts at: 252 252
Size: 4112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 613 613
Size: 4112
AlwNI CAG_nnn'CTG
Cuts at: 1417 2984 1417
Size: 1567 2545
ApaI G_GGCC'C
Cuts at: 926 926
Size: 4112
ApaLI G'TGCA_C
Cuts at: 793 2882 793
Size: 2089 2023
ApoI r'AATT_y
Cuts at: 1116 3465 3721 1116
Size: 2349 256 1507
Fragments arranged by size:
2349 1507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 1132 1132
Size: 4112
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 565 629 865 1128 1542 1790 1825 565
Size: 64 236 263 414 248 35 2852
Fragments arranged by size:
2852 414 263 248 236 64 35
BanII G_rGCy'C
Cuts at: 656 926 959 1126 2156 656
Size: 270 33 167 1030 2612
Fragments arranged by size:
2612 1030 270 167 33
BbeI G_GCGC'C
Cuts at: 633 1546 1794 633
Size: 913 248 2951
Fragments arranged by size:
2951 913 248
BbsI GAAGACnn'nnnn_
Cuts at: 730 888 1004 730
Size: 158 116 3838
Fragments arranged by size:
3838 158 116
BbvCI CC'TCA_GC
Cuts at: 814 814
Size: 4112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 555 589 662 696 555
Size: 34 73 34 3971
Fragments arranged by size:
3971 73 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 1632 1632
Size: 4112
BglI GCCn_nnn'nGGC
Cuts at: 227 638 227
Size: 411 3701
BglII A'GATC_T
Cuts at: 176 515 1627 3664 176
Size: 339 1112 2037 624
Fragments arranged by size:
2037 1112 624 339
BlpI GC'TnA_GC
Cuts at: 1224 1224
Size: 4112
Bme1580I G_kGCm'C
Cuts at: 453 568 797 834 868 926 1737 1830 2886
Size: 115 229 37 34 58 811 93 1056
Cuts at: 2886 453
Size: 1679
Fragments arranged by size:
1679 1056 811 229 115 93 58 37
34
BmrI ACTGGGnnnn_n'
Cuts at: 126 348 987 1324 1405 1740 3884 126
Size: 222 639 337 81 335 2144 354
Fragments arranged by size:
2144 639 354 337 335 222 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 805 148
Size: 657 3455
Bpu10I CC'TnA_GC
Cuts at: 814 814
Size: 4112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 2167 2659 2957 1288
Size: 879 492 298 2443
Fragments arranged by size:
2443 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3816 3816
Size: 4112
BsaAI yAC'GTr
Cuts at: 1465 1465
Size: 4112
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 630 1009 1543 1791 2493 3989 630
Size: 379 534 248 702 1496 753
Fragments arranged by size:
1496 753 702 534 379 248
BsaWI w'CCGG_w
Cuts at: 1301 1822 2500 2774 2921 3332 1301
Size: 521 678 274 147 411 2081
Fragments arranged by size:
2081 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 643 673 643
Size: 30 4082
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 861 1012 861
Size: 151 3961
BseYI C'CCAG_C
Cuts at: 278 1075 1537 2295 2872 3368 278
Size: 797 462 758 577 496 1022
Fragments arranged by size:
1022 797 758 577 496 462
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 273
Size: 4112
BsiEI CG_ry'CG
Cuts at: 104 164 494 1654 1700 2908 104
Size: 60 330 1160 46 1208 1308
Fragments arranged by size:
1308 1208 1160 330 60 46
BsiHKAI G_wGCw'C
Cuts at: 158 194 558 587 678 797 1126 1904 2094
Size: 36 364 29 91 119 329 778 190
Cuts at: 2094 2886 158
Size: 792 1384
Fragments arranged by size:
1384 792 778 364 329 190 119 91
36 29
Bsp1286I G_dGCh'C
Cuts at: 158 194 453 558 568 587 656 678 797
Size: 36 259 105 10 19 69 22 119
Cuts at: 797 834 868 926 959 1126 1737 1830 1904
Size: 37 34 58 33 167 611 93 74
Cuts at: 1904 2094 2156 2886 158
Size: 190 62 730 1384
Fragments arranged by size:
1384 730 611 259 190 167 119 105
93 74 69 62 58 37 36 34
33 22 19 10
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 3288 3288
Size: 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 788 806 1162 1376 1678 2059 788
Size: 18 356 214 302 381 2841
Fragments arranged by size:
2841 381 356 302 214 18
BsrBI CCG'CTC
Cuts at: 50 420 480 2404 2458 3942 4107 50
Size: 370 60 1924 54 1484 165 55
Fragments arranged by size:
1924 1484 370 165 60 55 54
BsrDI GCAATG_nn'
Cuts at: 2024 2024
Size: 4112
BsrFI r'CCGG_y
Cuts at: 226 456 2110 2291 2500 226
Size: 230 1654 181 209 1838
Fragments arranged by size:
1838 1654 230 209 181
BssSI C'ACGA_G
Cuts at: 691 2383 2741 691
Size: 1692 358 2062
Fragments arranged by size:
2062 1692 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1489 1489
Size: 4112
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstYI r'GATC_y
Cuts at: 1 176 515 1137 1604 1627 1962 2208 3220
Size: 175 339 622 467 23 335 246 1012
Cuts at: 3220 3306 3318 3664 1
Size: 86 12 346 449
Fragments arranged by size:
1012 622 467 449 346 339 335 246
175 86 23 12
BtgI C'CryG_G
Cuts at: 107 164 549 647 716 911 107
Size: 57 385 98 69 195 3308
Fragments arranged by size:
3308 385 195 98 69 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 451 1355 451
Size: 904 3208
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 703 1818 2676 703
Size: 1115 858 2139
Fragments arranged by size:
2139 1115 858
EaeI y'GGCC_r
Cuts at: 224 458 491 509 621 1053 1697 1871 2262
Size: 234 33 18 112 432 644 174 391
Cuts at: 2262 2289 3634 224
Size: 27 1345 702
Fragments arranged by size:
1345 702 644 432 391 234 174 112
33 27 18
EagI C'GGCC_G
Cuts at: 491 1697 491
Size: 1206 2906
EarI CTCTTCn'nnn_
Cuts at: 199 361 410 1033 2135 2345 199
Size: 162 49 623 1102 210 1966
Fragments arranged by size:
1966 1102 623 210 162 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2320 2630 2776 3922 2320
Size: 310 146 1146 2510
Fragments arranged by size:
2510 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 805 1937 2369 3116 3384 148
Size: 657 1132 432 747 268 876
Fragments arranged by size:
1132 876 747 657 432 268
EcoO109I rG'GnC_Cy
Cuts at: 465 922 923 1019 1251 465
Size: 457 1 96 232 3326
Fragments arranged by size:
3326 457 232 96 1
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 747 2131 747
Size: 1384 2728
FspI TGC'GCA
Cuts at: 544 1893 544
Size: 1349 2763
HaeII r_GCGC'y
Cuts at: 487 633 1546 1794 2816 3795 487
Size: 146 913 248 1022 979 804
Fragments arranged by size:
1022 979 913 804 248 146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 1954 1984 1986 2016 238
Size: 32 1684 30 2 30 2334
Fragments arranged by size:
2334 1684 32 30 30 2
HincII GTy'rAC
Cuts at: 1151 1151
Size: 4112
KasI G'GCGC_C
Cuts at: 629 1542 1790 629
Size: 913 248 2951
Fragments arranged by size:
2951 913 248
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 735 2783 2967 735
Size: 2048 184 1880
Fragments arranged by size:
2048 1880 184
MscI TGG'CCA
Cuts at: 511 623 1055 1873 511
Size: 112 432 818 2750
Fragments arranged by size:
2750 818 432 112
MslI CAynn'nnrTG
Cuts at: 742 748 2101 2228 742
Size: 6 1353 127 2626
Fragments arranged by size:
2626 1353 127 6
MspA1I CmG'CkG
Cuts at: 551 1223 1537 1897 2910 3155 551
Size: 672 314 360 1013 245 1508
Fragments arranged by size:
1508 1013 672 360 314 245
NaeI GCC'GGC
Cuts at: 228 458 2293 228
Size: 230 1835 2047
Fragments arranged by size:
2047 1835 230
NarI GG'CG_CC
Cuts at: 630 1543 1791 630
Size: 913 248 2951
Fragments arranged by size:
2951 913 248
NcoI C'CATG_G
Cuts at: 107 647 107
Size: 540 3572
NgoMIV G'CCGG_C
Cuts at: 226 456 2291 226
Size: 230 1835 2047
Fragments arranged by size:
2047 1835 230
NspI r_CATG'y
Cuts at: 1165 1665 2572 1165
Size: 500 907 2705
Fragments arranged by size:
2705 907 500
PasI CC'CwG_GG
Cuts at: 861 861
Size: 4112
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PfoI T'CCnGG_A
Cuts at: 125 149 1031 125
Size: 24 882 3206
Fragments arranged by size:
3206 882 24
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PpuMI rG'GwC_Cy
Cuts at: 465 465
Size: 4112
PspOMI G'GGCC_C
Cuts at: 922 922
Size: 4112
PstI C_TGCA'G
Cuts at: 350 1159 1844 350
Size: 809 685 2618
Fragments arranged by size:
2618 809 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 1537 1897 1537
Size: 360 3752
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SacII CC_GC'GG
Cuts at: 552 552
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 199 410 2135 2345 199
Size: 211 1725 210 1966
Fragments arranged by size:
1966 1725 211 210
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 3629 3629
Size: 4112
SexAI A'CCwGG_T
Cuts at: 143 890 143
Size: 747 3365
SfcI C'TryA_G
Cuts at: 33 346 1155 1840 2833 3024 33
Size: 313 809 685 993 191 1121
Fragments arranged by size:
1121 993 809 685 313 191
SfoI GGC'GCC
Cuts at: 631 1544 1792 631
Size: 913 248 2951
Fragments arranged by size:
2951 913 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 1134 1134
Size: 4112
SmlI C'TyrA_G
Cuts at: 1267 2182 2674 2936 1267
Size: 915 492 262 2443
Fragments arranged by size:
2443 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
StuI AGG'CCT
Cuts at: 313 313
Size: 4112
StyI C'CwwG_G
Cuts at: 107 461 647 1246 1600 107
Size: 354 186 599 354 2619
Fragments arranged by size:
2619 599 354 354 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 330 384 1695 1828 2287 2496 100
Size: 230 54 1311 133 459 209 1716
Fragments arranged by size:
1716 1311 459 230 209 133 54
TatI w'GTAC_w
Cuts at: 3627 3627
Size: 4112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4080
Tth111I GACn'n_nGTC
Cuts at: 145 892 1909 145
Size: 747 1017 2348
Fragments arranged by size:
2348 1017 747
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 519 1607 519
Size: 1088 3024
XmaI C'CCGG_G
Cuts at: 1132 1132
Size: 4112
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI