Restriction map for
pF1KB7137
(Circular) (Six-base) MAPSORT of: pF1KB7137.seq Check: 963 from: 1 to: 4097
pF1KB7137 4097 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 889 889
Size: 4097
AccI GT'mk_AC
Cuts at: 1135 1135
Size: 4097
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1068 1922 2354 3101 3369 859
Size: 209 854 432 747 268 1587
Fragments arranged by size:
1587 854 747 432 268 209
AflIII A'CryG_T
Cuts at: 1449 2553 3530 1449
Size: 1104 977 2016
Fragments arranged by size:
2016 1104 977
AgeI A'CCGG_T
Cuts at: 524 2485 524
Size: 1961 2136
AleI CACnn'nnGTG
Cuts at: 601 901 601
Size: 300 3797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 555 555
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 1402 2969 1402
Size: 1567 2530
ApaI G_GGCC'C
Cuts at: 276 276
Size: 4097
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 1101 3450 3706 1101
Size: 2349 256 1492
Fragments arranged by size:
2349 1492 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 1117 1117
Size: 4097
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 1113 1527 1775 1810 1113
Size: 414 248 35 3400
Fragments arranged by size:
3400 414 248 35
BanII G_rGCy'C
Cuts at: 276 326 433 1111 2141 276
Size: 50 107 678 1030 2232
Fragments arranged by size:
2232 1030 678 107 50
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BbsI GAAGACnn'nnnn_
Cuts at: 853 918 853
Size: 65 4032
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 1209 1209
Size: 4097
Bme1580I G_kGCm'C
Cuts at: 276 1722 1815 2871 276
Size: 1446 93 1056 1502
Fragments arranged by size:
1502 1446 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1309 1390 1725 3869 1309
Size: 81 335 2144 1537
Fragments arranged by size:
2144 1537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1028 2464 1028
Size: 1436 2661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 813
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 2152 2644 2942 1273
Size: 879 492 298 2428
Fragments arranged by size:
2428 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 954 3801 954
Size: 2847 1250
BsaAI yAC'GTr
Cuts at: 476 1450 476
Size: 974 3123
BsaBI GATnn'nnATC
Cuts at: 6 802 1611 3648 6
Size: 796 809 2037 455
Fragments arranged by size:
2037 809 796 455
BsaHI Gr'CG_yC
Cuts at: 1528 1776 2478 3974 1528
Size: 248 702 1496 1651
Fragments arranged by size:
1651 1496 702 248
BsaWI w'CCGG_w
Cuts at: 524 1286 1807 2485 2759 2906 3317 524
Size: 762 521 678 274 147 411 1304
Fragments arranged by size:
1304 762 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 493 493
Size: 4097
BseYI C'CCAG_C
Cuts at: 154 458 1522 2280 2857 3353 154
Size: 304 1064 758 577 496 898
Fragments arranged by size:
1064 898 758 577 496 304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 363
Size: 4097
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 221 433 1111 1889 2079 2871 221
Size: 212 678 778 190 792 1447
Fragments arranged by size:
1447 792 778 678 212 190
BsmBI CGTCTCn'nnnn_
Cuts at: 831 831
Size: 4097
Bsp1286I G_dGCh'C
Cuts at: 221 276 326 433 1111 1722 1815 1889 2079
Size: 55 50 107 678 611 93 74 190
Cuts at: 2079 2141 2871 221
Size: 62 730 1447
Fragments arranged by size:
1447 730 678 611 190 107 93 74
62 55 50
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 752 3273 752
Size: 2521 1576
BspMI ACCTGCnnnn'nnnn_
Cuts at: 889 1147 1361 1663 2044 889
Size: 258 214 302 381 2942
Fragments arranged by size:
2942 381 302 258 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 2009
Size: 4097
BsrFI r'CCGG_y
Cuts at: 524 2095 2276 2485 524
Size: 1571 181 209 2136
Fragments arranged by size:
2136 1571 209 181
BsrGI T'GTAC_A
Cuts at: 992 992
Size: 4097
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstEII G'GTnAC_C
Cuts at: 186 186
Size: 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 405 405
Size: 4097
BstYI r'GATC_y
Cuts at: 1 1122 1589 1612 1947 2193 3205 3291 3303
Size: 1121 467 23 335 246 1012 86 12
Cuts at: 3303 3649 1
Size: 346 449
Fragments arranged by size:
1121 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1063 1063
Size: 4097
BtgI C'CryG_G
Cuts at: 899 899
Size: 4097
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 789 2047 2228 2391 789
Size: 1258 181 163 2495
Fragments arranged by size:
2495 1258 181 163
BtsI GCAGTG_nn'
Cuts at: 1340 1340
Size: 4097
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 807 1803 2661 807
Size: 996 858 2243
Fragments arranged by size:
2243 996 858
EaeI y'GGCC_r
Cuts at: 302 407 593 629 1682 1856 2247 2274 3619
Size: 105 186 36 1053 174 391 27 1345
Cuts at: 3619 302
Size: 780
Fragments arranged by size:
1345 1053 780 391 186 174 105 36
27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 262 390 419 891 964 2120 2330 262
Size: 128 29 472 73 1156 210 2029
Fragments arranged by size:
2029 1156 472 210 128 73 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 781 2305 2615 2761 3907 781
Size: 1524 310 146 1146 971
Fragments arranged by size:
1524 1146 971 310 146
EcoICRI GAG'CTC
Cuts at: 431 1109 431
Size: 678 3419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 859 1068 1922 2354 3101 3369 813
Size: 46 209 854 432 747 268 1541
Fragments arranged by size:
1541 854 747 432 268 209 46
EcoNI CCTnn'n_nnAGG
Cuts at: 858 858
Size: 4097
EcoO109I rG'GnC_Cy
Cuts at: 272 273 702 1236 272
Size: 1 429 534 3133
Fragments arranged by size:
3133 534 429 1
EcoRI G'AATT_C
Cuts at: 1101 1101
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 406 1531 1779 2801 3780 406
Size: 1125 248 1022 979 723
Fragments arranged by size:
1125 1022 979 723 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 111 143 1939 1969 1971 2001 111
Size: 32 1796 30 2 30 2207
Fragments arranged by size:
2207 1796 32 30 30 2
HincII GTy'rAC
Cuts at: 624 744 1136 624
Size: 120 392 3585
Fragments arranged by size:
3585 392 120
HindIII A'AGCT_T
Cuts at: 1069 1069
Size: 4097
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 2768 2952 401
Size: 2367 184 1546
Fragments arranged by size:
2367 1546 184
MscI TGG'CCA
Cuts at: 304 409 595 1858 304
Size: 105 186 1263 2543
Fragments arranged by size:
2543 1263 186 105
MslI CAynn'nnrTG
Cuts at: 511 601 901 2086 2213 511
Size: 90 300 1185 127 2395
Fragments arranged by size:
2395 1185 300 127 90
MspA1I CmG'CkG
Cuts at: 248 549 587 922 1208 1522 1882 2895 3140
Size: 301 38 335 286 314 360 1013 245
Cuts at: 3140 248
Size: 1205
Fragments arranged by size:
1205 1013 360 335 314 301 286 245
38
NaeI GCC'GGC
Cuts at: 2278 2278
Size: 4097
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NgoMIV G'CCGG_C
Cuts at: 2276 2276
Size: 4097
NspI r_CATG'y
Cuts at: 912 1150 1650 2557 912
Size: 238 500 907 2452
Fragments arranged by size:
2452 907 500 238
PasI CC'CwG_GG
Cuts at: 319 319
Size: 4097
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PflMI CCAn_nnn'nTGG
Cuts at: 183 183
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PmlI CAC'GTG
Cuts at: 476 476
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PpuMI rG'GwC_Cy
Cuts at: 702 702
Size: 4097
PspOMI G'GGCC_C
Cuts at: 272 272
Size: 4097
PstI C_TGCA'G
Cuts at: 206 586 730 1144 1829 206
Size: 380 144 414 685 2474
Fragments arranged by size:
2474 685 414 380 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 248 1522 1882 248
Size: 1274 360 2463
Fragments arranged by size:
2463 1274 360
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 433 1111 433
Size: 678 3419
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 891 964 2120 2330 891
Size: 73 1156 210 2658
Fragments arranged by size:
2658 1156 210 73
SbfI CC_TGCA'GG
Cuts at: 206 1144 206
Size: 938 3159
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SfcI C'TryA_G
Cuts at: 33 202 582 726 957 1140 1825 2818 3009
Size: 169 380 144 231 183 685 993 191
Cuts at: 3009 33
Size: 1121
Fragments arranged by size:
1121 993 685 380 231 191 183 169
144
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 1119 1119
Size: 4097
SmlI C'TyrA_G
Cuts at: 1252 2167 2659 2921 1252
Size: 915 492 262 2428
Fragments arranged by size:
2428 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StyI C'CwwG_G
Cuts at: 160 299 398 1231 1585 160
Size: 139 99 833 354 2672
Fragments arranged by size:
2672 833 354 139 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 236 1680 1813 2272 2481 236
Size: 1444 133 459 209 1852
Fragments arranged by size:
1852 1444 459 209 133
TatI w'GTAC_w
Cuts at: 338 992 3612 338
Size: 654 2620 823
Fragments arranged by size:
2620 823 654
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4065
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1592 1592
Size: 4097
XmaI C'CCGG_G
Cuts at: 1117 1117
Size: 4097
XmnI GAAnn'nnTTC
Cuts at: 871 1072 871
Size: 201 3896
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BssHII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PfoI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI