Restriction map for
pF1KB7135
(Circular) (Six-base) MAPSORT of: pF1KB7135.seq Check: 2053 from: 1 to: 4076
pF1KB7135 4076 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 278 278
Size: 4076
AccI GT'mk_AC
Cuts at: 1114 1114
Size: 4076
Acc65I G'GTAC_C
Cuts at: 1092 1092
Size: 4076
AclI AA'CG_TT
Cuts at: 3916 3916
Size: 4076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1901 2333 3080 3348 1901
Size: 432 747 268 2629
Fragments arranged by size:
2629 747 432 268
AflIII A'CryG_T
Cuts at: 826 1428 2532 3509 826
Size: 602 1104 977 1393
Fragments arranged by size:
1393 1104 977 602
AgeI A'CCGG_T
Cuts at: 2464 2464
Size: 4076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 564 564
Size: 4076
AlwNI CAG_nnn'CTG
Cuts at: 1381 2948 1381
Size: 1567 2509
ApaLI G'TGCA_C
Cuts at: 355 2846 355
Size: 2491 1585
ApoI r'AATT_y
Cuts at: 139 1080 3429 3685 139
Size: 941 2349 256 530
Fragments arranged by size:
2349 941 530 256
AseI AT'TA_AT
Cuts at: 20 238 629 20
Size: 218 391 3467
Fragments arranged by size:
3467 391 218
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
AvaI C'yCGr_G
Cuts at: 1096 1096
Size: 4076
BamHI G'GATC_C
Cuts at: 788 1101 788
Size: 313 3763
BanI G'GyrC_C
Cuts at: 1092 1506 1754 1789 1092
Size: 414 248 35 3379
Fragments arranged by size:
3379 414 248 35
BanII G_rGCy'C
Cuts at: 1090 2120 1090
Size: 1030 3046
BbeI G_GCGC'C
Cuts at: 1510 1758 1510
Size: 248 3828
BbsI GAAGACnn'nnnn_
Cuts at: 711 813 711
Size: 102 3974
BciVI GTATCCnnnnn_n'
Cuts at: 1974 2735 4076 1974
Size: 761 1341 1974
Fragments arranged by size:
1974 1341 761
BclI T'GATC_A
Cuts at: 1596 1596
Size: 4076
BglII A'GATC_T
Cuts at: 1591 3628 1591
Size: 2037 2039
BlpI GC'TnA_GC
Cuts at: 1188 1188
Size: 4076
Bme1580I G_kGCm'C
Cuts at: 359 1701 1794 2850 359
Size: 1342 93 1056 1585
Fragments arranged by size:
1585 1342 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 722 1288 1369 1704 3848 722
Size: 566 81 335 2144 950
Fragments arranged by size:
2144 950 566 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 1252 2131 2623 2921 804
Size: 448 879 492 298 1959
Fragments arranged by size:
1959 879 492 448 298
BsaI GGTCTCn'nnnn_
Cuts at: 3780 3780
Size: 4076
BsaAI yAC'GTr
Cuts at: 1429 1429
Size: 4076
BsaBI GATnn'nnATC
Cuts at: 6 1590 3627 6
Size: 1584 2037 455
Fragments arranged by size:
2037 1584 455
BsaHI Gr'CG_yC
Cuts at: 1507 1755 2457 3953 1507
Size: 248 702 1496 1630
Fragments arranged by size:
1630 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1265 1786 2464 2738 2885 3296 1265
Size: 521 678 274 147 411 2045
Fragments arranged by size:
2045 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 352
Size: 4076
BseYI C'CCAG_C
Cuts at: 551 1501 2259 2836 3332 551
Size: 950 758 577 496 1295
Fragments arranged by size:
1295 950 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1618 1664 2872 104
Size: 1514 46 1208 1308
Fragments arranged by size:
1514 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 359 1090 1868 2058 2850 359
Size: 731 778 190 792 1585
Fragments arranged by size:
1585 792 778 731 190
Bsp1286I G_dGCh'C
Cuts at: 359 1090 1701 1794 1868 2058 2120 2850 359
Size: 731 611 93 74 190 62 730 1585
Fragments arranged by size:
1585 731 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3296 3296
Size: 4076
BspHI T'CATG_A
Cuts at: 3252 3252
Size: 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 1126 1340 1642 2023 278
Size: 848 214 302 381 2331
Fragments arranged by size:
2331 848 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2368 2422 3906 4071 50
Size: 2318 54 1484 165 55
Fragments arranged by size:
2318 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 342 464 476 1988 342
Size: 122 12 1512 2430
Fragments arranged by size:
2430 1512 122 12
BsrFI r'CCGG_y
Cuts at: 2074 2255 2464 2074
Size: 181 209 3686
Fragments arranged by size:
3686 209 181
BsrGI T'GTAC_A
Cuts at: 938 938
Size: 4076
BssSI C'ACGA_G
Cuts at: 2347 2705 2347
Size: 358 3718
BstAPI GCAn_nnn'nTGC
Cuts at: 1453 1453
Size: 4076
BstBI TT'CG_AA
Cuts at: 2437 2437
Size: 4076
BstYI r'GATC_y
Cuts at: 1 788 1101 1568 1591 1926 2172 3184 3270
Size: 787 313 467 23 335 246 1012 86
Cuts at: 3270 3282 3628 1
Size: 12 346 449
Fragments arranged by size:
1012 787 467 449 346 335 313 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4076
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2026 2207 2370 2026
Size: 181 163 3732
Fragments arranged by size:
3732 181 163
BtsI GCAGTG_nn'
Cuts at: 700 1319 700
Size: 619 3457
DraI TTT'AAA
Cuts at: 1075 1075
Size: 4076
DrdI GACnn_nn'nnGTC
Cuts at: 1782 2640 1782
Size: 858 3218
EaeI y'GGCC_r
Cuts at: 842 1661 1835 2226 2253 3598 842
Size: 819 174 391 27 1345 1320
Fragments arranged by size:
1345 1320 819 391 174 27
EagI C'GGCC_G
Cuts at: 1661 1661
Size: 4076
EarI CTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2284 2594 2740 3886 2284
Size: 310 146 1146 2474
Fragments arranged by size:
2474 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1088 1088
Size: 4076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1901 2333 3080 3348 1901
Size: 432 747 268 2629
Fragments arranged by size:
2629 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1215 1215
Size: 4076
EcoRI G'AATT_C
Cuts at: 139 1080 139
Size: 941 3135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 401 2095 401
Size: 1694 2382
FspI TGC'GCA
Cuts at: 1857 1857
Size: 4076
HaeII r_GCGC'y
Cuts at: 872 1510 1758 2780 3759 872
Size: 638 248 1022 979 1189
Fragments arranged by size:
1189 1022 979 638 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1918 1948 1950 1980 1918
Size: 30 2 30 4014
Fragments arranged by size:
4014 30 30 2
HincII GTy'rAC
Cuts at: 593 1115 593
Size: 522 3554
KasI G'GCGC_C
Cuts at: 1506 1754 1506
Size: 248 3828
KpnI G_GTAC'C
Cuts at: 1096 1096
Size: 4076
MluI A'CGCG_T
Cuts at: 3509 3509
Size: 4076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 185 709 2747 2931 185
Size: 524 2038 184 1330
Fragments arranged by size:
2038 1330 524 184
MscI TGG'CCA
Cuts at: 844 1837 844
Size: 993 3083
MslI CAynn'nnrTG
Cuts at: 2065 2192 2065
Size: 127 3949
MspA1I CmG'CkG
Cuts at: 1187 1501 1861 2874 3119 1187
Size: 314 360 1013 245 2144
Fragments arranged by size:
2144 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 4076
NarI GG'CG_CC
Cuts at: 1507 1755 1507
Size: 248 3828
NcoI C'CATG_G
Cuts at: 107 107
Size: 4076
NdeI CA'TA_TG
Cuts at: 465 882 465
Size: 417 3659
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 4076
NspI r_CATG'y
Cuts at: 830 1129 1629 2536 830
Size: 299 500 907 2370
Fragments arranged by size:
2370 907 500 299
PciI A'CATG_T
Cuts at: 826 2532 826
Size: 1706 2370
PflMI CCAn_nnn'nTGG
Cuts at: 351 351
Size: 4076
PmeI GTTT'AAAC
Cuts at: 1075 1075
Size: 4076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 4044
PstI C_TGCA'G
Cuts at: 1123 1808 1123
Size: 685 3391
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4076
PvuII CAG'CTG
Cuts at: 1501 1861 1501
Size: 360 3716
RsrII CG'GwC_CG
Cuts at: 2271 2271
Size: 4076
SacI G_AGCT'C
Cuts at: 1090 1090
Size: 4076
SalI G'TCGA_C
Cuts at: 1113 1113
Size: 4076
SapI GCTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
SbfI CC_TGCA'GG
Cuts at: 1123 1123
Size: 4076
ScaI AGT'ACT
Cuts at: 3593 3593
Size: 4076
SfcI C'TryA_G
Cuts at: 33 1016 1119 1804 2797 2988 33
Size: 983 103 685 993 191 1121
Fragments arranged by size:
1121 993 983 685 191 103
SfoI GGC'GCC
Cuts at: 1508 1756 1508
Size: 248 3828
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
SmaI CCC'GGG
Cuts at: 1098 1098
Size: 4076
SmlI C'TyrA_G
Cuts at: 783 1231 2146 2638 2900 783
Size: 448 915 492 262 1959
Fragments arranged by size:
1959 915 492 448 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4076
SphI G_CATG'C
Cuts at: 1129 1629 1129
Size: 500 3576
StyI C'CwwG_G
Cuts at: 107 1210 1564 107
Size: 1103 354 2619
Fragments arranged by size:
2619 1103 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1659 1792 2251 2460 1659
Size: 133 459 209 3275
Fragments arranged by size:
3275 459 209 133
TatI w'GTAC_w
Cuts at: 217 938 3591 217
Size: 721 2653 702
Fragments arranged by size:
2653 721 702
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4044
Tth111I GACn'n_nGTC
Cuts at: 1873 1873
Size: 4076
XbaI T'CTAG_A
Cuts at: 1107 1107
Size: 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1571 1571
Size: 4076
XmaI C'CCGG_G
Cuts at: 1096 1096
Size: 4076
XmnI GAAnn'nnTTC
Cuts at: 681 681
Size: 4076
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI