Restriction map for
pF1KB7134
(Circular) (Six-base) MAPSORT of: pF1KB7134.seq Check: 5496 from: 1 to: 4073
pF1KB7134 4073 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 1111 1111
Size: 4073
Acc65I G'GTAC_C
Cuts at: 1089 1089
Size: 4073
AclI AA'CG_TT
Cuts at: 3913 3913
Size: 4073
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1898 2330 3077 3345 1898
Size: 432 747 268 2626
Fragments arranged by size:
2626 747 432 268
AflIII A'CryG_T
Cuts at: 1425 2529 3506 1425
Size: 1104 977 1992
Fragments arranged by size:
1992 1104 977
AgeI A'CCGG_T
Cuts at: 2461 2461
Size: 4073
AleI CACnn'nnGTG
Cuts at: 139 839 139
Size: 700 3373
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 724 756 724
Size: 32 4041
AlwNI CAG_nnn'CTG
Cuts at: 1378 2945 1378
Size: 1567 2506
ApaLI G'TGCA_C
Cuts at: 2843 2843
Size: 4073
ApoI r'AATT_y
Cuts at: 1077 3426 3682 1077
Size: 2349 256 1468
Fragments arranged by size:
2349 1468 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4073
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
AvaI C'yCGr_G
Cuts at: 371 1093 371
Size: 722 3351
BamHI G'GATC_C
Cuts at: 559 1098 559
Size: 539 3534
BanI G'GyrC_C
Cuts at: 982 1089 1503 1751 1786 982
Size: 107 414 248 35 3269
Fragments arranged by size:
3269 414 248 107 35
BanII G_rGCy'C
Cuts at: 1087 2117 1087
Size: 1030 3043
BbeI G_GCGC'C
Cuts at: 1507 1755 1507
Size: 248 3825
BbsI GAAGACnn'nnnn_
Cuts at: 234 234
Size: 4073
BciVI GTATCCnnnnn_n'
Cuts at: 163 1971 2732 4073 163
Size: 1808 761 1341 163
Fragments arranged by size:
1808 1341 761 163
BclI T'GATC_A
Cuts at: 590 1593 590
Size: 1003 3070
BglII A'GATC_T
Cuts at: 1588 3625 1588
Size: 2037 2036
BlpI GC'TnA_GC
Cuts at: 1185 1185
Size: 4073
Bme1580I G_kGCm'C
Cuts at: 1698 1791 2847 1698
Size: 93 1056 2924
Fragments arranged by size:
2924 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1285 1366 1701 3845 1285
Size: 81 335 2144 1513
Fragments arranged by size:
2144 1513 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 2128 2620 2918 1249
Size: 879 492 298 2404
Fragments arranged by size:
2404 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3777 3777
Size: 4073
BsaAI yAC'GTr
Cuts at: 1426 1426
Size: 4073
BsaBI GATnn'nnATC
Cuts at: 6 538 1587 3624 6
Size: 532 1049 2037 455
Fragments arranged by size:
2037 1049 532 455
BsaHI Gr'CG_yC
Cuts at: 1504 1752 2454 3950 1504
Size: 248 702 1496 1627
Fragments arranged by size:
1627 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1262 1783 2461 2735 2882 3293 1262
Size: 521 678 274 147 411 2042
Fragments arranged by size:
2042 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1052 1082 1052
Size: 30 4043
BseYI C'CCAG_C
Cuts at: 1498 2256 2833 3329 1498
Size: 758 577 496 2242
Fragments arranged by size:
2242 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 253
Size: 4073
BsiEI CG_ry'CG
Cuts at: 104 1615 1661 2869 104
Size: 1511 46 1208 1308
Fragments arranged by size:
1511 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 512 1087 1865 2055 2847 512
Size: 575 778 190 792 1738
Fragments arranged by size:
1738 792 778 575 190
Bsp1286I G_dGCh'C
Cuts at: 512 1087 1698 1791 1865 2055 2117 2847 512
Size: 575 611 93 74 190 62 730 1738
Fragments arranged by size:
1738 730 611 575 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3293 3293
Size: 4073
BspHI T'CATG_A
Cuts at: 3249 3249
Size: 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 953 1123 1337 1639 2020 953
Size: 170 214 302 381 3006
Fragments arranged by size:
3006 381 302 214 170
BsrBI CCG'CTC
Cuts at: 50 804 2365 2419 3903 4068 50
Size: 754 1561 54 1484 165 55
Fragments arranged by size:
1561 1484 754 165 55 54
BsrDI GCAATG_nn'
Cuts at: 224 1985 224
Size: 1761 2312
BsrFI r'CCGG_y
Cuts at: 2071 2252 2461 2071
Size: 181 209 3683
Fragments arranged by size:
3683 209 181
BsrGI T'GTAC_A
Cuts at: 885 885
Size: 4073
BssSI C'ACGA_G
Cuts at: 2344 2702 2344
Size: 358 3715
BstAPI GCAn_nnn'nTGC
Cuts at: 1450 1450
Size: 4073
BstBI TT'CG_AA
Cuts at: 2434 2434
Size: 4073
BstXI CCAn_nnnn'nTGG
Cuts at: 315 450 717 315
Size: 135 267 3671
Fragments arranged by size:
3671 267 135
BstYI r'GATC_y
Cuts at: 1 344 559 1098 1565 1588 1923 2169 3181
Size: 343 215 539 467 23 335 246 1012
Cuts at: 3181 3267 3279 3625 1
Size: 86 12 346 449
Fragments arranged by size:
1012 539 467 449 346 343 335 246
215 86 23 12
BtgI C'CryG_G
Cuts at: 455 555 455
Size: 100 3973
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2023 2204 2367 2023
Size: 181 163 3729
Fragments arranged by size:
3729 181 163
BtsI GCAGTG_nn'
Cuts at: 490 512 577 1316 490
Size: 22 65 739 3247
Fragments arranged by size:
3247 739 65 22
DraI TTT'AAA
Cuts at: 1072 1072
Size: 4073
DrdI GACnn_nn'nnGTC
Cuts at: 1779 2637 1779
Size: 858 3215
EaeI y'GGCC_r
Cuts at: 452 476 1658 1832 2223 2250 3595 452
Size: 24 1182 174 391 27 1345 930
Fragments arranged by size:
1345 1182 930 391 174 27 24
EagI C'GGCC_G
Cuts at: 1658 1658
Size: 4073
EarI CTCTTCn'nnn_
Cuts at: 2096 2306 2096
Size: 210 3863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2281 2591 2737 3883 2281
Size: 310 146 1146 2471
Fragments arranged by size:
2471 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1085 1085
Size: 4073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1898 2330 3077 3345 1898
Size: 432 747 268 2626
Fragments arranged by size:
2626 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1212 1212
Size: 4073
EcoRI G'AATT_C
Cuts at: 1077 1077
Size: 4073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 4073
FspI TGC'GCA
Cuts at: 1854 1854
Size: 4073
HaeII r_GCGC'y
Cuts at: 1507 1755 2777 3756 1507
Size: 248 1022 979 1824
Fragments arranged by size:
1824 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1915 1945 1947 1977 1915
Size: 30 2 30 4011
Fragments arranged by size:
4011 30 30 2
HincII GTy'rAC
Cuts at: 1112 1112
Size: 4073
KasI G'GCGC_C
Cuts at: 1503 1751 1503
Size: 248 3825
KpnI G_GTAC'C
Cuts at: 1093 1093
Size: 4073
MluI A'CGCG_T
Cuts at: 3506 3506
Size: 4073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2744 2928 2744
Size: 184 3889
MscI TGG'CCA
Cuts at: 454 478 1834 454
Size: 24 1356 2693
Fragments arranged by size:
2693 1356 24
MslI CAynn'nnrTG
Cuts at: 139 617 650 727 839 841 2062 2189 139
Size: 478 33 77 112 2 1221 127 2023
Fragments arranged by size:
2023 1221 478 127 112 77 33 2
MspA1I CmG'CkG
Cuts at: 557 1034 1184 1498 1858 2871 3116 557
Size: 477 150 314 360 1013 245 1514
Fragments arranged by size:
1514 1013 477 360 314 245 150
NaeI GCC'GGC
Cuts at: 2254 2254
Size: 4073
NarI GG'CG_CC
Cuts at: 1504 1752 1504
Size: 248 3825
NcoI C'CATG_G
Cuts at: 455 455
Size: 4073
NgoMIV G'CCGG_C
Cuts at: 2252 2252
Size: 4073
NspI r_CATG'y
Cuts at: 133 300 1126 1626 2533 133
Size: 167 826 500 907 1673
Fragments arranged by size:
1673 907 826 500 167
PciI A'CATG_T
Cuts at: 2529 2529
Size: 4073
PflMI CCAn_nnn'nTGG
Cuts at: 871 871
Size: 4073
PmeI GTTT'AAAC
Cuts at: 1072 1072
Size: 4073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 4041
PstI C_TGCA'G
Cuts at: 507 1120 1805 507
Size: 613 685 2775
Fragments arranged by size:
2775 685 613
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4073
PvuII CAG'CTG
Cuts at: 1034 1498 1858 1034
Size: 464 360 3249
Fragments arranged by size:
3249 464 360
RsrII CG'GwC_CG
Cuts at: 2268 2268
Size: 4073
SacI G_AGCT'C
Cuts at: 1087 1087
Size: 4073
SacII CC_GC'GG
Cuts at: 558 558
Size: 4073
SalI G'TCGA_C
Cuts at: 1110 1110
Size: 4073
SapI GCTCTTCn'nnn_
Cuts at: 2096 2306 2096
Size: 210 3863
SbfI CC_TGCA'GG
Cuts at: 1120 1120
Size: 4073
ScaI AGT'ACT
Cuts at: 3590 3590
Size: 4073
SfcI C'TryA_G
Cuts at: 33 503 1116 1801 2794 2985 33
Size: 470 613 685 993 191 1121
Fragments arranged by size:
1121 993 685 613 470 191
SfoI GGC'GCC
Cuts at: 1505 1753 1505
Size: 248 3825
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
SmaI CCC'GGG
Cuts at: 1095 1095
Size: 4073
SmlI C'TyrA_G
Cuts at: 1228 2143 2635 2897 1228
Size: 915 492 262 2404
Fragments arranged by size:
2404 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4073
SphI G_CATG'C
Cuts at: 300 1126 1626 300
Size: 826 500 2747
Fragments arranged by size:
2747 826 500
StuI AGG'CCT
Cuts at: 391 814 391
Size: 423 3650
StyI C'CwwG_G
Cuts at: 455 809 1207 1561 455
Size: 354 398 354 2967
Fragments arranged by size:
2967 398 354 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 503 922 1656 1789 2248 2457 503
Size: 419 734 133 459 209 2119
Fragments arranged by size:
2119 734 459 419 209 133
TatI w'GTAC_w
Cuts at: 885 3588 885
Size: 2703 1370
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 812 844 3590 3622 812
Size: 32 2746 32 1263
Fragments arranged by size:
2746 1263 32 32
Tth111I GACn'n_nGTC
Cuts at: 1870 1870
Size: 4073
XbaI T'CTAG_A
Cuts at: 1104 1104
Size: 4073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 181 210 447 1568 181
Size: 29 237 1121 2686
Fragments arranged by size:
2686 1121 237 29
XmaI C'CCGG_G
Cuts at: 1093 1093
Size: 4073
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI